Reviewed, UniProtKB/Swiss-Prot P51913 (ENOA_CHICK)

Names and origin

Protein names

Recommended name:
    Alpha-enolase
    EC=4.2.1.11
Alternative name(s):
    2-phospho-D-glycerate hydro-lyase
    Phosphopyruvate hydratase

Gene names

Name:

ENO1

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	434 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O.
Cofactor	Magnesium. Required for catalysis and for stabilizing the dimer By similarity.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the enolase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Lyase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro
Cellular component	phosphopyruvate hydratase complex Inferred from electronic annotation. Source: InterPro
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphopyruvate hydratase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 434

433

Alpha-enolase

PRO_0000134101

Regions

Region

370 – 373

4

Substrate binding By similarity

Sites

Active site

210

1

Proton donor By similarity

Active site

343

1

Proton acceptor By similarity

Metal binding

245

1

Magnesium By similarity

Metal binding

293

1

Magnesium By similarity

Metal binding

318

1

Magnesium By similarity

Binding site

158

1

Substrate By similarity

Binding site

167

1

Substrate By similarity

Binding site

293

1

Substrate By similarity

Binding site

318

1

Substrate By similarity

Binding site

394

1

Substrate By similarity

Secondary structure

1

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434

Helix Strand Turn

Details...

Beta strand

4 – 11

8

Beta strand

17 – 25

9

Beta strand

28 – 33

6

Turn

53 – 55

3

Helix

56 – 58

3

Helix

62 – 70

9

Helix

72 – 79

8

Helix

86 – 97

12

Turn

103 – 105

3

Helix

107 – 124

18

Helix

129 – 137

9

Beta strand

149 – 153

5

Helix

155 – 157

3

Beta strand

158 – 161

4

Beta strand

166 – 170

5

Helix

177 – 199

23

Helix

201 – 203

3

Helix

219 – 233

15

Turn

236 – 238

3

Beta strand

240 – 244

5

Helix

247 – 250

4

Turn

258 – 261

4

Helix

266 – 268

3

Helix

272 – 285

14

Beta strand

288 – 292

5

Helix

300 – 308

9

Beta strand

311 – 317

7

Turn

318 – 322

5

Helix

324 – 332

9

Beta strand

337 – 341

5

Helix

343 – 346

4

Helix

349 – 361

13

Beta strand

365 – 369

5

Helix

379 – 386

8

Beta strand

390 – 393

4

Helix

400 – 416

17

Helix

417 – 419

3

Helix

424 – 426

3

Helix

430 – 432

3

Sequences

	Sequence		Length	Mass (Da)
	P51913-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 81428BB175FD7400	FASTA	434	47,305
	10 20 30 40 50 60 MSILKIHARE IFDSRGNPTV EVDLYTNKGL FRAAVPSGAS TGIYEALELR DNDKTRYLGK 70 80 90 100 110 120 GVSKAVEHVN KTIAPALISK NVNVVEQEKI DKLMLEMDGT ENKSKFGANA ILGVSLAVCK 130 140 150 160 170 180 AGAAEKGVPL YRHIADLAGN PEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGADTFKE 190 200 210 220 230 240 AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEALELLKTA IGKAGYSDKV 250 260 270 280 290 300 VIGMDVAASE FYRDGKYDLD FKSPDDPSRY ISPDQLADLY LGFVKNYPVV SIEDPFDQDD 310 320 330 340 350 360 WAAWKKFTAS VGIQVVGDDL TVTNPKRIAK AVEEKSCNCL LLKVNQIGSV TESLQACKLA 370 380 390 400 410 420 QSNGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEELGSK 430 ARFAGRNFRN PRIN

References

[1]

"Chicken alpha-enolase but not beta-enolase has a Src-dependent tyrosine-phosphorylation site: cDNA cloning and nucleotide sequence analysis." Tanaka M., Maeda K., Nakashima K. J. Biochem. 117:554-559(1995) [PubMed: 7629021] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: White leghorn. Tissue: Kidney.

Cross-references

Sequence databases

EMBLGenBankDDBJ

D37900 mRNA. Translation: BAA07132.1.

PIR

JC4186.

RefSeq

NP_990451.1.

UniGene

Gga.1383

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2GUA	model	-	A	1-434	[»]

SMR

P51913. Positions 2-433.

ModBase

Search...

Genome annotation databases

Ensembl

ENSGALG00000002377. Gallus gallus. [Contig view]

GeneID

396017.

KEGG

gga:396017.

Phylogenomic databases

HOVERGEN

P51913.

Family and domain databases

InterPro

IPR000941. Enolase.
[Graphical view]

PANTHER

PTHR11902. Enolase. 1 hit.

Pfam

PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
[Graphical view]

PIRSF

PIRSF001400. Enolase. 1 hit.

PRINTS

PR00148. ENOLASE.

ProDom

PD000902. Enolase. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR01060. eno. 1 hit.

PROSITE

PS00164. ENOLASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ENOA_CHICK

Accession

Primary (citable) accession number: P51913

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)