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P51912

- AAAT_MOUSE

UniProt

P51912 - AAAT_MOUSE

Protein

Neutral amino acid transporter B(0)

Gene

Slc1a5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 2 (15 Jul 1998)
      Previous versions | rss
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    Functioni

    Sodium-dependent amino acids transporter that has a broad substrate specificity, with a preference for zwitterionic amino acids. It accepts as substrates all neutral amino acids, including glutamine, asparagine, and branched-chain and aromatic amino acids, and excludes methylated, anionic, and cationic amino acids. May also be activated by insulin.1 Publication

    GO - Molecular functioni

    1. neutral amino acid transmembrane transporter activity Source: MGI
    2. sodium:dicarboxylate symporter activity Source: InterPro

    GO - Biological processi

    1. neutral amino acid transport Source: GOC

    Keywords - Biological processi

    Symport, Transport

    Protein family/group databases

    TCDBi2.A.23.3.2. the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neutral amino acid transporter B(0)
    Short name:
    ATB(0)
    Alternative name(s):
    ASC-like Na(+)-dependent neutral amino acid transporter ASCT2
    Insulin-activated amino acid transporter
    Sodium-dependent neutral amino acid transporter type 2
    Solute carrier family 1 member 5
    Gene namesi
    Name:Slc1a5
    Synonyms:Aaat, Asct2, Slc1a7
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:105305. Slc1a5.

    Subcellular locationi

    Cell membrane Curated; Multi-pass membrane protein Curated. Melanosome By similarity

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. melanosome Source: UniProtKB-SubCell
    3. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 553553Neutral amino acid transporter B(0)PRO_0000202083Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Glycosylationi165 – 1651N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi228 – 2281N-linked (GlcNAc...)Sequence Analysis
    Modified residuei505 – 5051PhosphoserineBy similarity
    Modified residuei506 – 5061Phosphoserine1 Publication
    Modified residuei518 – 5181Phosphoserine1 Publication

    Keywords - PTMi

    Acetylation, Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiP51912.
    PaxDbiP51912.
    PRIDEiP51912.

    PTM databases

    PhosphoSiteiP51912.

    Expressioni

    Tissue specificityi

    Highly expressed in adipose tissue, lower levels seen in lung. Not expressed in brain, kidney, liver or heart and only traces are detected in spleen, thymus and skeletal muscle.

    Developmental stagei

    Expressed is induced during differentiation of preadipocytes into adipocytes.

    Gene expression databases

    GenevestigatoriP51912.

    Structurei

    3D structure databases

    ProteinModelPortaliP51912.
    SMRiP51912. Positions 92-494.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 5151CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini153 – 23684ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei52 – 7221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei98 – 11821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei132 – 15221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei237 – 25721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei278 – 29821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei318 – 33821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei348 – 36821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei389 – 40921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei411 – 43121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei438 – 45821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1301.
    HOVERGENiHBG000080.
    InParanoidiP51912.

    Family and domain databases

    Gene3Di1.10.3860.10. 2 hits.
    InterProiIPR001991. Na-dicarboxylate_symporter.
    IPR018107. Na-dicarboxylate_symporter_CS.
    [Graphical view]
    PANTHERiPTHR11958. PTHR11958. 1 hit.
    PfamiPF00375. SDF. 1 hit.
    [Graphical view]
    PRINTSiPR00173. EDTRNSPORT.
    SUPFAMiSSF118215. SSF118215. 2 hits.
    PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
    PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P51912-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAVDPPKADP KGVAVDSSRR CPALGSREDQ SAKAGGCCGS RDRVRRCIRA    50
    NLLVLLTVAA VVAGVGLGLG VSAAGGADAL GPARLTRFAF PGELLLRLLK 100
    MIILPLVVCS LIGGAASLDP SALGRVGAWA LLFFLVTTLL ASALGVGLAL 150
    ALKPGAAVTA ITSINDSVVD PCARSAPTKE ALDSFLDLVR NIFPSNLVSA 200
    AFRSFATSYE PKDNSCKIPQ SCIQREINST MVQLLCEVEG MNILGLVVFA 250
    IVFGVALRKL GPEGELLIRF FNSFNDATMV LVSWIMWYAP VGILFLVASK 300
    IVEMKDVRQL FISLGKYILC CLLGHAIHGL LVLPLIYFLF TRKNPYRFLW 350
    GIMTPLATAF GTSSSSATLP LMMKCVEEKN GVAKHISRFI LPIGATVNMD 400
    GAALFQCVAA VFIAQLNGVS LDFVKIITIL VTATASSVGA AGIPAGGVLT 450
    LAIILEAVSL PVKDISLILA VDWLVDRSCT VLNVEGDAFG AGLLQSYVDR 500
    TKMPSSEPEL IQVKNEVSLN PLPLATEEGN PLLKQYQGPT GDSSATFEKE 550
    SVM 553
    Length:553
    Mass (Da):58,483
    Last modified:July 15, 1998 - v2
    Checksum:iD25EBC982FBDE158
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti13 – 219VAVDSSRRC → SSGGFHRNGG in AAA66264. (PubMed:7702599)Curated
    Sequence conflicti131 – 15424LLFFL…LALKP → ALFPGHHTARVGARRGFGPG AEA in AAA66264. (PubMed:7702599)CuratedAdd
    BLAST
    Sequence conflicti181 – 1811A → V in AAA66264. (PubMed:7702599)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42115 mRNA. Translation: AAA66264.1.
    D85044 mRNA. Translation: BAA12716.1.
    PIRiJC4149.
    UniGeneiMm.1056.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42115 mRNA. Translation: AAA66264.1 .
    D85044 mRNA. Translation: BAA12716.1 .
    PIRi JC4149.
    UniGenei Mm.1056.

    3D structure databases

    ProteinModelPortali P51912.
    SMRi P51912. Positions 92-494.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    TCDBi 2.A.23.3.2. the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family.

    PTM databases

    PhosphoSitei P51912.

    Proteomic databases

    MaxQBi P51912.
    PaxDbi P51912.
    PRIDEi P51912.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    MGIi MGI:105305. Slc1a5.

    Phylogenomic databases

    eggNOGi COG1301.
    HOVERGENi HBG000080.
    InParanoidi P51912.

    Miscellaneous databases

    PROi P51912.
    SOURCEi Search...

    Gene expression databases

    Genevestigatori P51912.

    Family and domain databases

    Gene3Di 1.10.3860.10. 2 hits.
    InterProi IPR001991. Na-dicarboxylate_symporter.
    IPR018107. Na-dicarboxylate_symporter_CS.
    [Graphical view ]
    PANTHERi PTHR11958. PTHR11958. 1 hit.
    Pfami PF00375. SDF. 1 hit.
    [Graphical view ]
    PRINTSi PR00173. EDTRNSPORT.
    SUPFAMi SSF118215. SSF118215. 2 hits.
    PROSITEi PS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
    PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Expression of a novel insulin-activated amino acid transporter gene during differentiation of 3T3-L1 preadipocytes into adipocytes."
      Liao K., Lane M.D.
      Biochem. Biophys. Res. Commun. 208:1008-1015(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
      Tissue: Adipose tissue.
    2. "Cloning and functional characterization of a system ASC-like Na+-dependent neutral amino acid transporter."
      Utsunomiya-Tate N., Endou H., Kanai Y.
      J. Biol. Chem. 271:14883-14890(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: JCL:ICR.
      Tissue: Testis.
    3. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-506 AND SER-518, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiAAAT_MOUSE
    AccessioniPrimary (citable) accession number: P51912
    Secondary accession number(s): Q61326
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: July 15, 1998
    Last modified: October 1, 2014
    This is version 96 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3