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P51912 (AAAT_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neutral amino acid transporter B(0)

Short name=ATB(0)
Alternative name(s):
ASC-like Na(+)-dependent neutral amino acid transporter ASCT2
Insulin-activated amino acid transporter
Sodium-dependent neutral amino acid transporter type 2
Solute carrier family 1 member 5
Gene names
Name:Slc1a5
Synonyms:Aaat, Asct2, Slc1a7
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length553 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Insulin-dependent transporter for L-serine and, to a lesser extent, uptake of L-alanine and L-glutamate.

Subcellular location

Membrane; Multi-pass membrane protein Probable. Melanosome By similarity.

Tissue specificity

Highly expressed in adipose tissue, lower levels seen in lung. Not expressed in brain, kidney, liver or heart and only traces are detected in spleen, thymus and skeletal muscle.

Developmental stage

Expressed is induced during differentiation of preadipocytes into adipocytes.

Sequence similarities

Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A5 subfamily. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 553553Neutral amino acid transporter B(0)
PRO_0000202083

Regions

Topological domain1 – 5151Cytoplasmic Potential
Transmembrane52 – 7221Helical; Potential
Transmembrane98 – 11821Helical; Potential
Transmembrane132 – 15221Helical; Potential
Topological domain153 – 23684Extracellular Potential
Transmembrane237 – 25721Helical; Potential
Transmembrane278 – 29821Helical; Potential
Transmembrane318 – 33821Helical; Potential
Transmembrane348 – 36821Helical; Potential
Transmembrane389 – 40921Helical; Potential
Transmembrane411 – 43121Helical; Potential
Transmembrane438 – 45821Helical; Potential

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue5051Phosphoserine By similarity
Modified residue5061Phosphoserine Ref.3
Modified residue5181Phosphoserine Ref.3
Glycosylation1651N-linked (GlcNAc...) Potential
Glycosylation2281N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict13 – 219VAVDSSRRC → SSGGFHRNGG in AAA66264. Ref.1
Sequence conflict131 – 15424LLFFL…LALKP → ALFPGHHTARVGARRGFGPG AEA in AAA66264. Ref.1
Sequence conflict1811A → V in AAA66264. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P51912 [UniParc].

Last modified July 15, 1998. Version 2.
Checksum: D25EBC982FBDE158

FASTA55358,483
        10         20         30         40         50         60 
MAVDPPKADP KGVAVDSSRR CPALGSREDQ SAKAGGCCGS RDRVRRCIRA NLLVLLTVAA 

        70         80         90        100        110        120 
VVAGVGLGLG VSAAGGADAL GPARLTRFAF PGELLLRLLK MIILPLVVCS LIGGAASLDP 

       130        140        150        160        170        180 
SALGRVGAWA LLFFLVTTLL ASALGVGLAL ALKPGAAVTA ITSINDSVVD PCARSAPTKE 

       190        200        210        220        230        240 
ALDSFLDLVR NIFPSNLVSA AFRSFATSYE PKDNSCKIPQ SCIQREINST MVQLLCEVEG 

       250        260        270        280        290        300 
MNILGLVVFA IVFGVALRKL GPEGELLIRF FNSFNDATMV LVSWIMWYAP VGILFLVASK 

       310        320        330        340        350        360 
IVEMKDVRQL FISLGKYILC CLLGHAIHGL LVLPLIYFLF TRKNPYRFLW GIMTPLATAF 

       370        380        390        400        410        420 
GTSSSSATLP LMMKCVEEKN GVAKHISRFI LPIGATVNMD GAALFQCVAA VFIAQLNGVS 

       430        440        450        460        470        480 
LDFVKIITIL VTATASSVGA AGIPAGGVLT LAIILEAVSL PVKDISLILA VDWLVDRSCT 

       490        500        510        520        530        540 
VLNVEGDAFG AGLLQSYVDR TKMPSSEPEL IQVKNEVSLN PLPLATEEGN PLLKQYQGPT 

       550 
GDSSATFEKE SVM 

« Hide

References

« Hide 'large scale' references
[1]"Expression of a novel insulin-activated amino acid transporter gene during differentiation of 3T3-L1 preadipocytes into adipocytes."
Liao K., Lane M.D.
Biochem. Biophys. Res. Commun. 208:1008-1015(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Adipose tissue.
[2]"Cloning and functional characterization of a system ASC-like Na+-dependent neutral amino acid transporter."
Utsunomiya-Tate N., Endou H., Kanai Y.
J. Biol. Chem. 271:14883-14890(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: JCL:ICR.
Tissue: Testis.
[3]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-506 AND SER-518, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L42115 mRNA. Translation: AAA66264.1.
D85044 mRNA. Translation: BAA12716.1.
PIRJC4149.
UniGeneMm.1056.

3D structure databases

ProteinModelPortalP51912.
SMRP51912. Positions 92-494.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

TCDB2.A.23.3.2. the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family.

PTM databases

PhosphoSiteP51912.

Proteomic databases

MaxQBP51912.
PaxDbP51912.
PRIDEP51912.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

MGIMGI:105305. Slc1a5.

Phylogenomic databases

eggNOGCOG1301.
HOVERGENHBG000080.
InParanoidP51912.

Gene expression databases

GenevestigatorP51912.

Family and domain databases

Gene3D1.10.3860.10. 2 hits.
InterProIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERPTHR11958. PTHR11958. 1 hit.
PfamPF00375. SDF. 1 hit.
[Graphical view]
PRINTSPR00173. EDTRNSPORT.
SUPFAMSSF118215. SSF118215. 2 hits.
PROSITEPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP51912.
SOURCESearch...

Entry information

Entry nameAAAT_MOUSE
AccessionPrimary (citable) accession number: P51912
Secondary accession number(s): Q61326
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 15, 1998
Last modified: June 11, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot