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Protein

Calponin-1

Gene

CNN1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity).By similarity

GO - Biological processi

  • actomyosin structure organization Source: InterPro
  • negative regulation of vascular smooth muscle cell proliferation Source: BHF-UCL
  • regulation of smooth muscle contraction Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Actin-binding, Calmodulin-binding

Enzyme and pathway databases

SIGNORiP51911.

Names & Taxonomyi

Protein namesi
Recommended name:
Calponin-1
Alternative name(s):
Basic calponin
Calponin H1, smooth muscle
Gene namesi
Name:CNN1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:2155. CNN1.

Subcellular locationi

GO - Cellular componenti

  • cytoskeleton Source: MGI
  • focal adhesion Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi1264.
OpenTargetsiENSG00000130176.
PharmGKBiPA26665.

Polymorphism and mutation databases

BioMutaiCNN1.
DMDMi2829431.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002047671 – 297Calponin-1Add BLAST297

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei170Phosphothreonine; by ROCK2By similarity1
Modified residuei175Phosphoserine; by ROCK2By similarity1
Modified residuei180Phosphothreonine; by ROCK2By similarity1
Modified residuei184Phosphothreonine; by ROCK2By similarity1
Modified residuei259Phosphothreonine; by ROCK2By similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP51911.
PaxDbiP51911.
PeptideAtlasiP51911.
PRIDEiP51911.

PTM databases

iPTMnetiP51911.
PhosphoSitePlusiP51911.

Expressioni

Tissue specificityi

Smooth muscle, and tissues containing significant amounts of smooth muscle.

Gene expression databases

BgeeiENSG00000130176.
CleanExiHS_CNN1.
ExpressionAtlasiP51911. baseline and differential.
GenevisibleiP51911. HS.

Organism-specific databases

HPAiCAB000007.
HPA014263.

Interactioni

Subunit structurei

Part of cGMP kinase signaling complex at least composed of ACTA2/alpha-actin, CNN1/calponin H1, PLN/phospholamban, PRKG1 and ITPR1.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
TFPTA0A024R4Q54EBI-2873130,EBI-11527449

Protein-protein interaction databases

BioGridi107664. 12 interactors.
IntActiP51911. 11 interactors.
STRINGi9606.ENSP00000252456.

Structurei

Secondary structure

1297
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi28 – 41Combined sources14
Helixi52 – 55Combined sources4
Helixi58 – 67Combined sources10
Beta strandi71 – 73Combined sources3
Helixi82 – 98Combined sources17
Helixi102 – 104Combined sources3
Helixi108 – 112Combined sources5
Helixi118 – 134Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WYPNMR-A20-142[»]
ProteinModelPortaliP51911.
SMRiP51911.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP51911.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 131CHPROSITE-ProRule annotationAdd BLAST104
Repeati164 – 189Calponin-like 1Add BLAST26
Repeati204 – 229Calponin-like 2Add BLAST26
Repeati243 – 268Calponin-like 3Add BLAST26

Sequence similaritiesi

Belongs to the calponin family.Curated
Contains 3 calponin-like repeats.PROSITE-ProRule annotation
Contains 1 CH (calponin-homology) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2046. Eukaryota.
COG5199. LUCA.
GeneTreeiENSGT00550000074447.
HOGENOMiHOG000232113.
HOVERGENiHBG005186.
InParanoidiP51911.
OMAiRQIFEPT.
OrthoDBiEOG091G0LY2.
PhylomeDBiP51911.
TreeFamiTF313921.

Family and domain databases

CDDicd00014. CH. 1 hit.
Gene3Di1.10.418.10. 1 hit.
InterProiIPR001997. Calponin/LIMCH1.
IPR000557. Calponin_repeat.
IPR001715. CH-domain.
IPR003096. SM22_calponin.
[Graphical view]
PfamiPF00402. Calponin. 3 hits.
PF00307. CH. 1 hit.
[Graphical view]
PRINTSiPR00889. CALPONIN.
PR00888. SM22CALPONIN.
SMARTiSM00033. CH. 1 hit.
[Graphical view]
SUPFAMiSSF47576. SSF47576. 1 hit.
PROSITEiPS01052. CALPONIN_1. 3 hits.
PS51122. CALPONIN_2. 3 hits.
PS50021. CH. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P51911-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSAHFNRGP AYGLSAEVKN KLAQKYDHQR EQELREWIEG VTGRRIGNNF
60 70 80 90 100
MDGLKDGIIL CEFINKLQPG SVKKINESTQ NWHQLENIGN FIKAITKYGV
110 120 130 140 150
KPHDIFEAND LFENTNHTQV QSTLLALASM AKTKGNKVNV GVKYAEKQER
160 170 180 190 200
KFEPGKLREG RNIIGLQMGT NKFASQQGMT AYGTRRHLYD PKLGTDQPLD
210 220 230 240 250
QATISLQMGT NKGASQAGMT APGTKRQIFE PGLGMEHCDT LNVSLQMGSN
260 270 280 290
KGASQRGMTV YGLPRQVYDP KYCLTPEYPE LGEPAHNHHA HNYYNSA
Length:297
Mass (Da):33,170
Last modified:November 1, 1997 - v2
Checksum:iA949573543BC246C
GO
Isoform 2 (identifier: P51911-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-21: MSSAHFNRGPAYGLSAEVKNK → M

Note: No experimental confirmation available.
Show »
Length:277
Mass (Da):31,055
Checksum:i4FD53B7974B60AC5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti57G → S in BAA12983 (Ref. 8) Curated1
Sequence conflicti149E → G in BAA12983 (Ref. 8) Curated1
Sequence conflicti170T → S in AAC51780 (PubMed:9332369).Curated1
Sequence conflicti266Q → P in BAA12983 (Ref. 8) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0569481 – 21MSSAH…EVKNK → M in isoform 2. 1 PublicationAdd BLAST21

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S80560 mRNA. Translation: AAB35751.1.
U37019 mRNA. Translation: AAC51780.1.
D17408 mRNA. Translation: BAA04231.1.
AK300837 mRNA. Translation: BAG62488.1.
AK313255 mRNA. Translation: BAG36065.1.
AC008481 Genomic DNA. No translation available.
CH471106 Genomic DNA. Translation: EAW84229.1.
BC022015 mRNA. Translation: AAH22015.1.
BC036307 mRNA. Translation: AAH36307.2.
D86058 mRNA. Translation: BAA12983.1.
D85611 Genomic DNA. Translation: BAA19538.1.
CCDSiCCDS12263.1. [P51911-1]
PIRiG02142.
JC4500.
RefSeqiNP_001290.2. NM_001299.5. [P51911-1]
NP_001295270.1. NM_001308341.1.
UniGeneiHs.465929.

Genome annotation databases

EnsembliENST00000252456; ENSP00000252456; ENSG00000130176. [P51911-1]
GeneIDi1264.
KEGGihsa:1264.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S80560 mRNA. Translation: AAB35751.1.
U37019 mRNA. Translation: AAC51780.1.
D17408 mRNA. Translation: BAA04231.1.
AK300837 mRNA. Translation: BAG62488.1.
AK313255 mRNA. Translation: BAG36065.1.
AC008481 Genomic DNA. No translation available.
CH471106 Genomic DNA. Translation: EAW84229.1.
BC022015 mRNA. Translation: AAH22015.1.
BC036307 mRNA. Translation: AAH36307.2.
D86058 mRNA. Translation: BAA12983.1.
D85611 Genomic DNA. Translation: BAA19538.1.
CCDSiCCDS12263.1. [P51911-1]
PIRiG02142.
JC4500.
RefSeqiNP_001290.2. NM_001299.5. [P51911-1]
NP_001295270.1. NM_001308341.1.
UniGeneiHs.465929.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WYPNMR-A20-142[»]
ProteinModelPortaliP51911.
SMRiP51911.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107664. 12 interactors.
IntActiP51911. 11 interactors.
STRINGi9606.ENSP00000252456.

PTM databases

iPTMnetiP51911.
PhosphoSitePlusiP51911.

Polymorphism and mutation databases

BioMutaiCNN1.
DMDMi2829431.

Proteomic databases

EPDiP51911.
PaxDbiP51911.
PeptideAtlasiP51911.
PRIDEiP51911.

Protocols and materials databases

DNASUi1264.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000252456; ENSP00000252456; ENSG00000130176. [P51911-1]
GeneIDi1264.
KEGGihsa:1264.

Organism-specific databases

CTDi1264.
DisGeNETi1264.
GeneCardsiCNN1.
HGNCiHGNC:2155. CNN1.
HPAiCAB000007.
HPA014263.
MIMi600806. gene.
neXtProtiNX_P51911.
OpenTargetsiENSG00000130176.
PharmGKBiPA26665.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2046. Eukaryota.
COG5199. LUCA.
GeneTreeiENSGT00550000074447.
HOGENOMiHOG000232113.
HOVERGENiHBG005186.
InParanoidiP51911.
OMAiRQIFEPT.
OrthoDBiEOG091G0LY2.
PhylomeDBiP51911.
TreeFamiTF313921.

Enzyme and pathway databases

SIGNORiP51911.

Miscellaneous databases

ChiTaRSiCNN1. human.
EvolutionaryTraceiP51911.
GenomeRNAii1264.
PROiP51911.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000130176.
CleanExiHS_CNN1.
ExpressionAtlasiP51911. baseline and differential.
GenevisibleiP51911. HS.

Family and domain databases

CDDicd00014. CH. 1 hit.
Gene3Di1.10.418.10. 1 hit.
InterProiIPR001997. Calponin/LIMCH1.
IPR000557. Calponin_repeat.
IPR001715. CH-domain.
IPR003096. SM22_calponin.
[Graphical view]
PfamiPF00402. Calponin. 3 hits.
PF00307. CH. 1 hit.
[Graphical view]
PRINTSiPR00889. CALPONIN.
PR00888. SM22CALPONIN.
SMARTiSM00033. CH. 1 hit.
[Graphical view]
SUPFAMiSSF47576. SSF47576. 1 hit.
PROSITEiPS01052. CALPONIN_1. 3 hits.
PS51122. CALPONIN_2. 3 hits.
PS50021. CH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCNN1_HUMAN
AccessioniPrimary (citable) accession number: P51911
Secondary accession number(s): B2R868
, B4DUX6, O00638, Q15416, Q8IY93, Q99438
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 1, 1997
Last modified: November 30, 2016
This is version 150 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.