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Protein

Excitatory amino acid transporter 3

Gene

Slc1a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transports L-glutamate, L- and D-aspartate and L-cystein. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium (By similarity). Negatively regulated by ARL6IP5 (PubMed:11242046).By similarity1 Publication

GO - Molecular functioni

  • cysteine transmembrane transporter activity Source: UniProtKB
  • glutamate:sodium symporter activity Source: RGD
  • glutamate binding Source: RGD
  • L-glutamate transmembrane transporter activity Source: RGD
  • sodium:dicarboxylate symporter activity Source: InterPro

GO - Biological processi

  • cysteine transport Source: UniProtKB
  • D-aspartate import Source: Ensembl
  • L-glutamate import Source: Ensembl
  • L-glutamate transport Source: RGD
  • positive regulation of heart rate Source: Ensembl
  • protein homooligomerization Source: RGD
Complete GO annotation...

Keywords - Biological processi

Symport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-210500. Glutamate Neurotransmitter Release Cycle.
R-RNO-425393. Transport of inorganic cations/anions and amino acids/oligopeptides.

Names & Taxonomyi

Protein namesi
Recommended name:
Excitatory amino acid transporter 3
Alternative name(s):
Excitatory amino-acid carrier 1
Sodium-dependent glutamate/aspartate transporter 3
Solute carrier family 1 member 1
Gene namesi
Name:Slc1a1
Synonyms:Eaac1, Eaat3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi3696. Slc1a1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1818CytoplasmicSequence analysisAdd
BLAST
Transmembranei19 – 3820HelicalSequence analysisAdd
BLAST
Transmembranei62 – 8221HelicalSequence analysisAdd
BLAST
Transmembranei94 – 11421HelicalSequence analysisAdd
BLAST
Topological domaini115 – 20894ExtracellularSequence analysisAdd
BLAST
Transmembranei209 – 22820HelicalSequence analysisAdd
BLAST
Transmembranei253 – 27220HelicalSequence analysisAdd
BLAST
Transmembranei289 – 30820HelicalSequence analysisAdd
BLAST
Transmembranei315 – 33420HelicalSequence analysisAdd
BLAST
Transmembranei361 – 38020HelicalSequence analysisAdd
BLAST
Transmembranei391 – 41020HelicalSequence analysisAdd
BLAST
Transmembranei419 – 43820HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL3113.
GuidetoPHARMACOLOGYi870.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 523523Excitatory amino acid transporter 3PRO_0000202067Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi128 – 1281N-linked (GlcNAc...)Sequence analysis
Glycosylationi178 – 1781N-linked (GlcNAc...)Sequence analysis
Glycosylationi194 – 1941N-linked (GlcNAc...)Sequence analysis
Modified residuei516 – 5161PhosphoserineBy similarity
Modified residuei521 – 5211PhosphoserineBy similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP51907.
PRIDEiP51907.

PTM databases

iPTMnetiP51907.
PhosphoSiteiP51907.
SwissPalmiP51907.

Expressioni

Tissue specificityi

Expressed to a high extent in brain and kidney, and to a lower extent in heart, lung and skeletal muscle.1 Publication

Gene expression databases

GenevisibleiP51907. RN.

Interactioni

Subunit structurei

Interacts with ARL6IP5/PRAF3.1 Publication

Protein-protein interaction databases

MINTiMINT-2839476.
STRINGi10116.ENSRNOP00000020272.

Chemistry

BindingDBiP51907.

Structurei

3D structure databases

ProteinModelPortaliP51907.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3787. Eukaryota.
COG1301. LUCA.
GeneTreeiENSGT00760000119117.
HOGENOMiHOG000208776.
HOVERGENiHBG000080.
InParanoidiP51907.
KOiK05612.
OMAiCEKKSYV.
OrthoDBiEOG7RV9G2.
PhylomeDBiP51907.
TreeFamiTF315206.

Family and domain databases

Gene3Di1.10.3860.10. 2 hits.
InterProiIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 1 hit.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 2 hits.
PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P51907-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKPTSSGCD WRRFLRNHWL LLSTVAAVVL GIVVGVLVRG HSELSNLDKF
60 70 80 90 100
YFAFPGEILM RMLKLVILPL IISSMITGVA ALDSNVSGKI GLRAVVYYFS
110 120 130 140 150
TTVIAVILGI VLVVSIKPGV TQKVNEINRT GKTPEVSTVD AMLDLIRNMF
160 170 180 190 200
PENLVQACFQ QYKTKREEVK PASDPGGNQT EVSVTTAMTT MSENKTKEYK
210 220 230 240 250
IVGLYSDGIN VLGLIIFCLV FGLVIGKMGE KGQILVDFFN ALSDATMKIV
260 270 280 290 300
QIIMCYMPIG ILFLIAGKII EVEDWEIFRK LGLYMATVLS GLAIHSLVVL
310 320 330 340 350
PLIYFIVVRK NPFRFALGMA QALLTALMIS SSSATLPVTF RCAEEKNHVD
360 370 380 390 400
KRITRFVLPV GATINMDGTA LYEAVAAVFI AQLNGMDLSI GQIITISITA
410 420 430 440 450
TAASIGAAGV PQAGLVTMVI VLSAVGLPAE DVTLIIAVDW LLDRFRTMVN
460 470 480 490 500
VLGDAFGTGI VEKLSKKELE QVDVSSEVNI VNPFALEPTI LDNEDSDTKK
510 520
SYVNGGFSVD KSDTISFTQT SQF
Length:523
Mass (Da):56,772
Last modified:October 1, 1996 - v1
Checksum:iD6783244E37640E4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti68 – 681L → M in BAA09849 (PubMed:8613726).Curated
Sequence conflicti93 – 931R → C in BAA09849 (PubMed:8613726).Curated
Sequence conflicti149 – 1513MFP → ILG in AAB09773 (PubMed:8747153).Curated
Sequence conflicti156 – 1561Q → E in AAB51161 (PubMed:8738164).Curated
Sequence conflicti203 – 2031G → C in AAB51161 (PubMed:8738164).Curated
Sequence conflicti218 – 2181C → F in AAB51161 (PubMed:8738164).Curated
Sequence conflicti279 – 2791R → C in AAC25030 (Ref. 7) Curated
Sequence conflicti309 – 3091R → T in AAB51161 (PubMed:8738164).Curated
Sequence conflicti383 – 3831L → V in BAA09849 (PubMed:8613726).Curated
Sequence conflicti424 – 4241A → P in AAF34319 (PubMed:8772431).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94255 mRNA. Translation: CAA63937.1.
U39555 mRNA. Translation: AAB09773.1.
D63772 mRNA. Translation: BAA09849.1.
L35558 mRNA. Translation: AAB51161.1.
BC061743 mRNA. Translation: AAH61743.1.
U21104 mRNA. Translation: AAF34319.1.
AF038571 mRNA. Translation: AAC25030.1.
RefSeqiNP_037164.3. NM_013032.3.
UniGeneiRn.6384.

Genome annotation databases

EnsembliENSRNOT00000020272; ENSRNOP00000020272; ENSRNOG00000014816.
GeneIDi25550.
KEGGirno:25550.
UCSCiRGD:3696. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94255 mRNA. Translation: CAA63937.1.
U39555 mRNA. Translation: AAB09773.1.
D63772 mRNA. Translation: BAA09849.1.
L35558 mRNA. Translation: AAB51161.1.
BC061743 mRNA. Translation: AAH61743.1.
U21104 mRNA. Translation: AAF34319.1.
AF038571 mRNA. Translation: AAC25030.1.
RefSeqiNP_037164.3. NM_013032.3.
UniGeneiRn.6384.

3D structure databases

ProteinModelPortaliP51907.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-2839476.
STRINGi10116.ENSRNOP00000020272.

Chemistry

BindingDBiP51907.
ChEMBLiCHEMBL3113.
GuidetoPHARMACOLOGYi870.

PTM databases

iPTMnetiP51907.
PhosphoSiteiP51907.
SwissPalmiP51907.

Proteomic databases

PaxDbiP51907.
PRIDEiP51907.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000020272; ENSRNOP00000020272; ENSRNOG00000014816.
GeneIDi25550.
KEGGirno:25550.
UCSCiRGD:3696. rat.

Organism-specific databases

CTDi6505.
RGDi3696. Slc1a1.

Phylogenomic databases

eggNOGiKOG3787. Eukaryota.
COG1301. LUCA.
GeneTreeiENSGT00760000119117.
HOGENOMiHOG000208776.
HOVERGENiHBG000080.
InParanoidiP51907.
KOiK05612.
OMAiCEKKSYV.
OrthoDBiEOG7RV9G2.
PhylomeDBiP51907.
TreeFamiTF315206.

Enzyme and pathway databases

ReactomeiR-RNO-210500. Glutamate Neurotransmitter Release Cycle.
R-RNO-425393. Transport of inorganic cations/anions and amino acids/oligopeptides.

Miscellaneous databases

PROiP51907.

Gene expression databases

GenevisibleiP51907. RN.

Family and domain databases

Gene3Di1.10.3860.10. 2 hits.
InterProiIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 1 hit.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 2 hits.
PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Cerebellum.
  2. "Neuronal high-affinity glutamate transport in the rat central nervous system."
    Kanai Y., Bhide P.G., Difiglia M., Hediger M.A.
    NeuroReport 6:2357-2362(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
  3. "Nerve injury enhances rat neuronal glutamate transporter expression: identification by differential display PCR."
    Kiryu S., Yao G.L., Morita N., Kato H., Kiyama H.
    J. Neurosci. 15:7872-7878(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Brain.
  4. "Characterization and distribution of the neuronal glutamate transporter EAAC1 in rat brain."
    Velaz-Faircloth M., McGraw T.S., Malandro M.S., Fremeau R.T. Jr., Kilberg M.S., Anderson K.J.
    Am. J. Physiol. 270:C67-C75(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Hippocampus.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.
  6. "Rat C6 and human astrocytic tumor cells express a neuronal type of glutamate transporter."
    Palos T.P., Ramachandran B., Boado R., Howard B.D.
    Brain Res. Mol. Brain Res. 37:297-303(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 156-368.
  7. Rome S., Mertani H.C., Lee K.O., Tome D.
    Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 267-445.
    Tissue: Intestine.
  8. "Modulation of the neuronal glutamate transporter EAAC1 by the interacting protein GTRAP3-18."
    Lin C.-L.G., Orlov I., Ruggiero A.M., Dykes-Hoberg M., Lee A., Jackson M., Rothstein J.D.
    Nature 410:84-88(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ARL6IP5.
  9. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiEAA3_RAT
AccessioniPrimary (citable) accession number: P51907
Secondary accession number(s): O88389, Q63727, Q9JJP8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 8, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.