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P51906 (EAA3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Excitatory amino acid transporter 3
Alternative name(s):
Excitatory amino-acid carrier 1
Sodium-dependent glutamate/aspartate transporter 3
Solute carrier family 1 member 1
Gene names
Name:Slc1a1
Synonyms:Eaac1, Eaat3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length523 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Negatively regulated by ARL6IP5.

Subunit structure

Interacts with ARL6IP5/PRAF3. Ref.5

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Expressed in brain, lung, kidney and skeletal muscle.

Sequence similarities

Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A1 subfamily. [View classification]

Binary interactions

With

Entry

#Exp.

IntAct

Notes

HccsP537025EBI-8580001,EBI-8579982

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 523523Excitatory amino acid transporter 3
PRO_0000202066

Regions

Topological domain1 – 1818Cytoplasmic Potential
Transmembrane19 – 3820Helical; Potential
Transmembrane62 – 8221Helical; Potential
Transmembrane94 – 11421Helical; Potential
Topological domain115 – 20894Extracellular Potential
Transmembrane209 – 22820Helical; Potential
Transmembrane253 – 27220Helical; Potential
Transmembrane289 – 30820Helical; Potential
Transmembrane315 – 33420Helical; Potential
Transmembrane361 – 38020Helical; Potential
Transmembrane391 – 41020Helical; Potential
Transmembrane419 – 43820Helical; Potential

Amino acid modifications

Glycosylation1281N-linked (GlcNAc...) Potential
Glycosylation1781N-linked (GlcNAc...) Potential
Glycosylation1941N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict1211T → A in AAB71742. Ref.2
Sequence conflict1391M → I in BAA07855. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P51906 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 46F35480465E5A82

FASTA52356,694
        10         20         30         40         50         60 
MGKPTSSGCD WRRFLRNHWL LLSTVAAVVL GIVLGVVVRG HSELSNLDKF YFAFPGEILM 

        70         80         90        100        110        120 
RMLKLVILPL IVSSMITGVA ALDSNVSGKI GLRAVVYYFS TTVIAVILGI VLVVSIKPGV 

       130        140        150        160        170        180 
TQKVNDINRT GKTPEVSTMD AMLDLIRNMF PENLVQACFQ QYKTKREEVK PVGDPGGNAT 

       190        200        210        220        230        240 
EVSVTTAMTT MSENKTKEYK IVGLYSDGIN VLGLIIFCLV FGLVIGKMGE KGQILVDFFN 

       250        260        270        280        290        300 
ALSDATMKIV QIIMCYMPIG ILFLIAGKII EVEDWEIFRK LGLYMATVLS GLAIHSLIVL 

       310        320        330        340        350        360 
PLLYFIVVRK NPFRFALGMA QALLTALMIS SSSATLPVTF RCAEEKNQVD KRITRFVLPV 

       370        380        390        400        410        420 
GATINMDGTA LYEAVAAVFI AQLNGLDLSI GQIVTISITA TAASIGAAGV PQAGLVTMVI 

       430        440        450        460        470        480 
VLSAVGLPAE DVTLIIAVDW LLDRFRTMVN VLGDAFGTGI VEKLSKKELE QMDVSSEVNI 

       490        500        510        520 
VNPFALEPTT LDNEDSDTKK SYVNGGFAVD KSDTISFTQT SQF 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of two glutamate transporter subtypes from mouse brain."
Mukainaka Y., Tanaka K., Hagiwara T., Wada K.
Biochim. Biophys. Acta 1244:233-237(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: JCL:ICR.
Tissue: Cerebellum.
[2]"Molecular cloning of a mouse glutamate transporter."
Peng J.-B., Guo L.-H.
Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Tissue: Brain.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[5]"Molecular cloning, gene structure, expression profile and functional characterization of the mouse glutamate transporter (EAAT3) interacting protein GTRAP3-18."
Butchbach M.E.R., Lai L., Lin C.-L.G.
Gene 292:81-90(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ARL6IP5.
Strain: C57BL/6J.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D43797 mRNA. Translation: BAA07855.1.
U73521 mRNA. Translation: AAB80694.1.
U75217 Genomic DNA. Translation: AAB71742.1.
CH466534 Genomic DNA. Translation: EDL41660.1.
BC065099 mRNA. Translation: AAH65099.1.
CCDSCCDS29727.1.
PIRS55677.
RefSeqNP_033225.1. NM_009199.2.
UniGeneMm.246670.

3D structure databases

ProteinModelPortalP51906.
SMRP51906. Positions 28-119, 211-463.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP51906. 1 interaction.
MINTMINT-2839452.
STRING10090.ENSMUSP00000025875.

PTM databases

PhosphoSiteP51906.

Proteomic databases

PaxDbP51906.
PRIDEP51906.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000025875; ENSMUSP00000025875; ENSMUSG00000024935.
GeneID20510.
KEGGmmu:20510.
UCSCuc008hcl.1. mouse.

Organism-specific databases

CTD6505.
MGIMGI:105083. Slc1a1.

Phylogenomic databases

eggNOGCOG1301.
GeneTreeENSGT00730000110358.
HOGENOMHOG000208776.
HOVERGENHBG000080.
InParanoidQ6P1F7.
KOK05612.
OMACIFLAQA.
OrthoDBEOG7RV9G2.
TreeFamTF315206.

Gene expression databases

BgeeP51906.
GenevestigatorP51906.

Family and domain databases

Gene3D1.10.3860.10. 2 hits.
InterProIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERPTHR11958. PTHR11958. 1 hit.
PfamPF00375. SDF. 1 hit.
[Graphical view]
PRINTSPR00173. EDTRNSPORT.
SUPFAMSSF118215. SSF118215. 2 hits.
PROSITEPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio298699.
PROP51906.
SOURCESearch...

Entry information

Entry nameEAA3_MOUSE
AccessionPrimary (citable) accession number: P51906
Secondary accession number(s): O35869, O35875, Q6P1F7
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot