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Protein

Excitatory amino acid transporter 3

Gene

Slc1a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium (By similarity). Negatively regulated by ARL6IP5 (PubMed:12119102).By similarity1 Publication

GO - Molecular functioni

  • cysteine transmembrane transporter activity Source: UniProtKB
  • glutamate:sodium symporter activity Source: MGI
  • glutamate binding Source: Ensembl
  • L-glutamate transmembrane transporter activity Source: MGI

GO - Biological processi

  • cysteine transport Source: UniProtKB
  • D-aspartate import Source: MGI
  • L-glutamate import Source: MGI
  • L-glutamate transmembrane transport Source: MGI
  • L-glutamate transport Source: MGI
  • positive regulation of heart rate Source: CACAO
  • protein homooligomerization Source: MGI
Complete GO annotation...

Keywords - Biological processi

Symport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-210500. Glutamate Neurotransmitter Release Cycle.
R-MMU-425393. Transport of inorganic cations/anions and amino acids/oligopeptides.

Names & Taxonomyi

Protein namesi
Recommended name:
Excitatory amino acid transporter 3
Alternative name(s):
Excitatory amino-acid carrier 1
Sodium-dependent glutamate/aspartate transporter 3
Solute carrier family 1 member 1
Gene namesi
Name:Slc1a1
Synonyms:Eaac1, Eaat3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:105083. Slc1a1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 18CytoplasmicSequence analysisAdd BLAST18
Transmembranei19 – 38HelicalSequence analysisAdd BLAST20
Transmembranei62 – 82HelicalSequence analysisAdd BLAST21
Transmembranei94 – 114HelicalSequence analysisAdd BLAST21
Topological domaini115 – 208ExtracellularSequence analysisAdd BLAST94
Transmembranei209 – 228HelicalSequence analysisAdd BLAST20
Transmembranei253 – 272HelicalSequence analysisAdd BLAST20
Transmembranei289 – 308HelicalSequence analysisAdd BLAST20
Transmembranei315 – 334HelicalSequence analysisAdd BLAST20
Transmembranei361 – 380HelicalSequence analysisAdd BLAST20
Transmembranei391 – 410HelicalSequence analysisAdd BLAST20
Transmembranei419 – 438HelicalSequence analysisAdd BLAST20

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002020661 – 523Excitatory amino acid transporter 3Add BLAST523

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi128N-linked (GlcNAc...)Sequence analysis1
Glycosylationi178N-linked (GlcNAc...)Sequence analysis1
Glycosylationi194N-linked (GlcNAc...)Sequence analysis1
Modified residuei516PhosphoserineCombined sources1
Modified residuei521PhosphoserineCombined sources1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP51906.
PaxDbiP51906.
PeptideAtlasiP51906.
PRIDEiP51906.

PTM databases

iPTMnetiP51906.
PhosphoSitePlusiP51906.

Expressioni

Tissue specificityi

Expressed in brain, lung, kidney and skeletal muscle.

Gene expression databases

BgeeiENSMUSG00000024935.
GenevisibleiP51906. MM.

Interactioni

Subunit structurei

Interacts with ARL6IP5 (PubMed:12119102, PubMed:18684713).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HccsP537025EBI-8580001,EBI-8579982

Protein-protein interaction databases

IntActiP51906. 1 interactor.
MINTiMINT-2839452.
STRINGi10090.ENSMUSP00000025875.

Structurei

3D structure databases

ProteinModelPortaliP51906.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3787. Eukaryota.
COG1301. LUCA.
GeneTreeiENSGT00760000119117.
HOGENOMiHOG000208776.
HOVERGENiHBG000080.
InParanoidiP51906.
KOiK05612.
OMAiALMISSX.
OrthoDBiEOG091G0UCE.
TreeFamiTF315206.

Family and domain databases

Gene3Di1.10.3860.10. 2 hits.
InterProiIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 1 hit.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 2 hits.
PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P51906-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKPTSSGCD WRRFLRNHWL LLSTVAAVVL GIVLGVVVRG HSELSNLDKF
60 70 80 90 100
YFAFPGEILM RMLKLVILPL IVSSMITGVA ALDSNVSGKI GLRAVVYYFS
110 120 130 140 150
TTVIAVILGI VLVVSIKPGV TQKVNDINRT GKTPEVSTMD AMLDLIRNMF
160 170 180 190 200
PENLVQACFQ QYKTKREEVK PVGDPGGNAT EVSVTTAMTT MSENKTKEYK
210 220 230 240 250
IVGLYSDGIN VLGLIIFCLV FGLVIGKMGE KGQILVDFFN ALSDATMKIV
260 270 280 290 300
QIIMCYMPIG ILFLIAGKII EVEDWEIFRK LGLYMATVLS GLAIHSLIVL
310 320 330 340 350
PLLYFIVVRK NPFRFALGMA QALLTALMIS SSSATLPVTF RCAEEKNQVD
360 370 380 390 400
KRITRFVLPV GATINMDGTA LYEAVAAVFI AQLNGLDLSI GQIVTISITA
410 420 430 440 450
TAASIGAAGV PQAGLVTMVI VLSAVGLPAE DVTLIIAVDW LLDRFRTMVN
460 470 480 490 500
VLGDAFGTGI VEKLSKKELE QMDVSSEVNI VNPFALEPTT LDNEDSDTKK
510 520
SYVNGGFAVD KSDTISFTQT SQF
Length:523
Mass (Da):56,694
Last modified:July 27, 2011 - v2
Checksum:i46F35480465E5A82
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti121T → A in AAB71742 (Ref. 2) Curated1
Sequence conflicti139M → I in BAA07855 (PubMed:7766664).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D43797 mRNA. Translation: BAA07855.1.
U73521 mRNA. Translation: AAB80694.1.
U75217 Genomic DNA. Translation: AAB71742.1.
CH466534 Genomic DNA. Translation: EDL41660.1.
BC065099 mRNA. Translation: AAH65099.1.
CCDSiCCDS29727.1.
PIRiS55677.
RefSeqiNP_033225.1. NM_009199.2.
UniGeneiMm.246670.

Genome annotation databases

EnsembliENSMUST00000025875; ENSMUSP00000025875; ENSMUSG00000024935.
GeneIDi20510.
KEGGimmu:20510.
UCSCiuc008hcl.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D43797 mRNA. Translation: BAA07855.1.
U73521 mRNA. Translation: AAB80694.1.
U75217 Genomic DNA. Translation: AAB71742.1.
CH466534 Genomic DNA. Translation: EDL41660.1.
BC065099 mRNA. Translation: AAH65099.1.
CCDSiCCDS29727.1.
PIRiS55677.
RefSeqiNP_033225.1. NM_009199.2.
UniGeneiMm.246670.

3D structure databases

ProteinModelPortaliP51906.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP51906. 1 interactor.
MINTiMINT-2839452.
STRINGi10090.ENSMUSP00000025875.

PTM databases

iPTMnetiP51906.
PhosphoSitePlusiP51906.

Proteomic databases

MaxQBiP51906.
PaxDbiP51906.
PeptideAtlasiP51906.
PRIDEiP51906.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025875; ENSMUSP00000025875; ENSMUSG00000024935.
GeneIDi20510.
KEGGimmu:20510.
UCSCiuc008hcl.1. mouse.

Organism-specific databases

CTDi6505.
MGIiMGI:105083. Slc1a1.

Phylogenomic databases

eggNOGiKOG3787. Eukaryota.
COG1301. LUCA.
GeneTreeiENSGT00760000119117.
HOGENOMiHOG000208776.
HOVERGENiHBG000080.
InParanoidiP51906.
KOiK05612.
OMAiALMISSX.
OrthoDBiEOG091G0UCE.
TreeFamiTF315206.

Enzyme and pathway databases

ReactomeiR-MMU-210500. Glutamate Neurotransmitter Release Cycle.
R-MMU-425393. Transport of inorganic cations/anions and amino acids/oligopeptides.

Miscellaneous databases

ChiTaRSiSlc1a1. mouse.
PROiP51906.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024935.
GenevisibleiP51906. MM.

Family and domain databases

Gene3Di1.10.3860.10. 2 hits.
InterProiIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 1 hit.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 2 hits.
PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEAA3_MOUSE
AccessioniPrimary (citable) accession number: P51906
Secondary accession number(s): O35869, O35875, Q6P1F7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.