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P51906

- EAA3_MOUSE

UniProt

P51906 - EAA3_MOUSE

Protein

Excitatory amino acid transporter 3

Gene

Slc1a1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Negatively regulated by ARL6IP5.

    GO - Molecular functioni

    1. glutamate:sodium symporter activity Source: MGI
    2. glutamate binding Source: Ensembl
    3. L-glutamate transmembrane transporter activity Source: MGI
    4. protein binding Source: IntAct
    5. sodium:dicarboxylate symporter activity Source: InterPro

    GO - Biological processi

    1. D-aspartate import Source: Ensembl
    2. L-glutamate import Source: Ensembl
    3. L-glutamate transport Source: MGI
    4. protein homooligomerization Source: MGI

    Keywords - Biological processi

    Symport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Excitatory amino acid transporter 3
    Alternative name(s):
    Excitatory amino-acid carrier 1
    Sodium-dependent glutamate/aspartate transporter 3
    Solute carrier family 1 member 1
    Gene namesi
    Name:Slc1a1
    Synonyms:Eaac1, Eaat3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:105083. Slc1a1.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: MGI

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 523523Excitatory amino acid transporter 3PRO_0000202066Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi128 – 1281N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi178 – 1781N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi194 – 1941N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiP51906.
    PRIDEiP51906.

    PTM databases

    PhosphoSiteiP51906.

    Expressioni

    Tissue specificityi

    Expressed in brain, lung, kidney and skeletal muscle.

    Gene expression databases

    BgeeiP51906.
    GenevestigatoriP51906.

    Interactioni

    Subunit structurei

    Interacts with ARL6IP5/PRAF3.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    HccsP537025EBI-8580001,EBI-8579982

    Protein-protein interaction databases

    IntActiP51906. 1 interaction.
    MINTiMINT-2839452.
    STRINGi10090.ENSMUSP00000025875.

    Structurei

    3D structure databases

    ProteinModelPortaliP51906.
    SMRiP51906. Positions 28-119, 211-463.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1818CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini115 – 20894ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei19 – 3820HelicalSequence AnalysisAdd
    BLAST
    Transmembranei62 – 8221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei94 – 11421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei209 – 22820HelicalSequence AnalysisAdd
    BLAST
    Transmembranei253 – 27220HelicalSequence AnalysisAdd
    BLAST
    Transmembranei289 – 30820HelicalSequence AnalysisAdd
    BLAST
    Transmembranei315 – 33420HelicalSequence AnalysisAdd
    BLAST
    Transmembranei361 – 38020HelicalSequence AnalysisAdd
    BLAST
    Transmembranei391 – 41020HelicalSequence AnalysisAdd
    BLAST
    Transmembranei419 – 43820HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1301.
    GeneTreeiENSGT00730000110358.
    HOGENOMiHOG000208776.
    HOVERGENiHBG000080.
    InParanoidiQ6P1F7.
    KOiK05612.
    OMAiCIFLAQA.
    OrthoDBiEOG7RV9G2.
    TreeFamiTF315206.

    Family and domain databases

    Gene3Di1.10.3860.10. 2 hits.
    InterProiIPR001991. Na-dicarboxylate_symporter.
    IPR018107. Na-dicarboxylate_symporter_CS.
    [Graphical view]
    PANTHERiPTHR11958. PTHR11958. 1 hit.
    PfamiPF00375. SDF. 1 hit.
    [Graphical view]
    PRINTSiPR00173. EDTRNSPORT.
    SUPFAMiSSF118215. SSF118215. 2 hits.
    PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
    PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P51906-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGKPTSSGCD WRRFLRNHWL LLSTVAAVVL GIVLGVVVRG HSELSNLDKF    50
    YFAFPGEILM RMLKLVILPL IVSSMITGVA ALDSNVSGKI GLRAVVYYFS 100
    TTVIAVILGI VLVVSIKPGV TQKVNDINRT GKTPEVSTMD AMLDLIRNMF 150
    PENLVQACFQ QYKTKREEVK PVGDPGGNAT EVSVTTAMTT MSENKTKEYK 200
    IVGLYSDGIN VLGLIIFCLV FGLVIGKMGE KGQILVDFFN ALSDATMKIV 250
    QIIMCYMPIG ILFLIAGKII EVEDWEIFRK LGLYMATVLS GLAIHSLIVL 300
    PLLYFIVVRK NPFRFALGMA QALLTALMIS SSSATLPVTF RCAEEKNQVD 350
    KRITRFVLPV GATINMDGTA LYEAVAAVFI AQLNGLDLSI GQIVTISITA 400
    TAASIGAAGV PQAGLVTMVI VLSAVGLPAE DVTLIIAVDW LLDRFRTMVN 450
    VLGDAFGTGI VEKLSKKELE QMDVSSEVNI VNPFALEPTT LDNEDSDTKK 500
    SYVNGGFAVD KSDTISFTQT SQF 523
    Length:523
    Mass (Da):56,694
    Last modified:July 27, 2011 - v2
    Checksum:i46F35480465E5A82
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti121 – 1211T → A in AAB71742. 1 PublicationCurated
    Sequence conflicti139 – 1391M → I in BAA07855. (PubMed:7766664)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D43797 mRNA. Translation: BAA07855.1.
    U73521 mRNA. Translation: AAB80694.1.
    U75217 Genomic DNA. Translation: AAB71742.1.
    CH466534 Genomic DNA. Translation: EDL41660.1.
    BC065099 mRNA. Translation: AAH65099.1.
    CCDSiCCDS29727.1.
    PIRiS55677.
    RefSeqiNP_033225.1. NM_009199.2.
    UniGeneiMm.246670.

    Genome annotation databases

    EnsembliENSMUST00000025875; ENSMUSP00000025875; ENSMUSG00000024935.
    GeneIDi20510.
    KEGGimmu:20510.
    UCSCiuc008hcl.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D43797 mRNA. Translation: BAA07855.1 .
    U73521 mRNA. Translation: AAB80694.1 .
    U75217 Genomic DNA. Translation: AAB71742.1 .
    CH466534 Genomic DNA. Translation: EDL41660.1 .
    BC065099 mRNA. Translation: AAH65099.1 .
    CCDSi CCDS29727.1.
    PIRi S55677.
    RefSeqi NP_033225.1. NM_009199.2.
    UniGenei Mm.246670.

    3D structure databases

    ProteinModelPortali P51906.
    SMRi P51906. Positions 28-119, 211-463.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P51906. 1 interaction.
    MINTi MINT-2839452.
    STRINGi 10090.ENSMUSP00000025875.

    PTM databases

    PhosphoSitei P51906.

    Proteomic databases

    PaxDbi P51906.
    PRIDEi P51906.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000025875 ; ENSMUSP00000025875 ; ENSMUSG00000024935 .
    GeneIDi 20510.
    KEGGi mmu:20510.
    UCSCi uc008hcl.1. mouse.

    Organism-specific databases

    CTDi 6505.
    MGIi MGI:105083. Slc1a1.

    Phylogenomic databases

    eggNOGi COG1301.
    GeneTreei ENSGT00730000110358.
    HOGENOMi HOG000208776.
    HOVERGENi HBG000080.
    InParanoidi Q6P1F7.
    KOi K05612.
    OMAi CIFLAQA.
    OrthoDBi EOG7RV9G2.
    TreeFami TF315206.

    Miscellaneous databases

    NextBioi 298699.
    PROi P51906.
    SOURCEi Search...

    Gene expression databases

    Bgeei P51906.
    Genevestigatori P51906.

    Family and domain databases

    Gene3Di 1.10.3860.10. 2 hits.
    InterProi IPR001991. Na-dicarboxylate_symporter.
    IPR018107. Na-dicarboxylate_symporter_CS.
    [Graphical view ]
    PANTHERi PTHR11958. PTHR11958. 1 hit.
    Pfami PF00375. SDF. 1 hit.
    [Graphical view ]
    PRINTSi PR00173. EDTRNSPORT.
    SUPFAMi SSF118215. SSF118215. 2 hits.
    PROSITEi PS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
    PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of two glutamate transporter subtypes from mouse brain."
      Mukainaka Y., Tanaka K., Hagiwara T., Wada K.
      Biochim. Biophys. Acta 1244:233-237(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: JCL:ICR.
      Tissue: Cerebellum.
    2. "Molecular cloning of a mouse glutamate transporter."
      Peng J.-B., Guo L.-H.
      Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Tissue: Brain.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    5. "Molecular cloning, gene structure, expression profile and functional characterization of the mouse glutamate transporter (EAAT3) interacting protein GTRAP3-18."
      Butchbach M.E.R., Lai L., Lin C.-L.G.
      Gene 292:81-90(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ARL6IP5.
      Strain: C57BL/6J.
      Tissue: Brain.

    Entry informationi

    Entry nameiEAA3_MOUSE
    AccessioniPrimary (citable) accession number: P51906
    Secondary accession number(s): O35869, O35875, Q6P1F7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 110 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3