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Protein

Excitatory amino acid transporter 3

Gene

Slc1a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium (By similarity). Negatively regulated by ARL6IP5 (PubMed:12119102).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • cysteine transmembrane transport Source: MGI
  • cysteine transport Source: UniProtKB
  • D-aspartate import Source: MGI
  • L-glutamate import Source: MGI
  • L-glutamate transmembrane transport Source: MGI
  • L-glutamate transport Source: MGI
  • positive regulation of heart rate Source: CACAO
  • protein homooligomerization Source: MGI
Complete GO annotation...

Keywords - Biological processi

Symport, Transport

Enzyme and pathway databases

ReactomeiREACT_277017. Transport of inorganic cations/anions and amino acids/oligopeptides.
REACT_287745. Glutamate Neurotransmitter Release Cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Excitatory amino acid transporter 3
Alternative name(s):
Excitatory amino-acid carrier 1
Sodium-dependent glutamate/aspartate transporter 3
Solute carrier family 1 member 1
Gene namesi
Name:Slc1a1
Synonyms:Eaac1, Eaat3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:105083. Slc1a1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1818CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei19 – 3820HelicalSequence AnalysisAdd
BLAST
Transmembranei62 – 8221HelicalSequence AnalysisAdd
BLAST
Transmembranei94 – 11421HelicalSequence AnalysisAdd
BLAST
Topological domaini115 – 20894ExtracellularSequence AnalysisAdd
BLAST
Transmembranei209 – 22820HelicalSequence AnalysisAdd
BLAST
Transmembranei253 – 27220HelicalSequence AnalysisAdd
BLAST
Transmembranei289 – 30820HelicalSequence AnalysisAdd
BLAST
Transmembranei315 – 33420HelicalSequence AnalysisAdd
BLAST
Transmembranei361 – 38020HelicalSequence AnalysisAdd
BLAST
Transmembranei391 – 41020HelicalSequence AnalysisAdd
BLAST
Transmembranei419 – 43820HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 523523Excitatory amino acid transporter 3PRO_0000202066Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi128 – 1281N-linked (GlcNAc...)Sequence Analysis
Glycosylationi178 – 1781N-linked (GlcNAc...)Sequence Analysis
Glycosylationi194 – 1941N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP51906.
PaxDbiP51906.
PRIDEiP51906.

PTM databases

PhosphoSiteiP51906.

Expressioni

Tissue specificityi

Expressed in brain, lung, kidney and skeletal muscle.

Gene expression databases

BgeeiP51906.
ExpressionAtlasiP51906. baseline and differential.
GenevisibleiP51906. MM.

Interactioni

Subunit structurei

Interacts with ARL6IP5/PRAF3.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
HccsP537025EBI-8580001,EBI-8579982

Protein-protein interaction databases

IntActiP51906. 1 interaction.
MINTiMINT-2839452.
STRINGi10090.ENSMUSP00000025875.

Structurei

3D structure databases

ProteinModelPortaliP51906.
SMRiP51906. Positions 28-119, 211-463.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1301.
GeneTreeiENSGT00760000119117.
HOGENOMiHOG000208776.
HOVERGENiHBG000080.
InParanoidiP51906.
KOiK05612.
OMAiVSAVGWH.
OrthoDBiEOG7RV9G2.
TreeFamiTF315206.

Family and domain databases

Gene3Di1.10.3860.10. 2 hits.
InterProiIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 1 hit.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 2 hits.
PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P51906-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKPTSSGCD WRRFLRNHWL LLSTVAAVVL GIVLGVVVRG HSELSNLDKF
60 70 80 90 100
YFAFPGEILM RMLKLVILPL IVSSMITGVA ALDSNVSGKI GLRAVVYYFS
110 120 130 140 150
TTVIAVILGI VLVVSIKPGV TQKVNDINRT GKTPEVSTMD AMLDLIRNMF
160 170 180 190 200
PENLVQACFQ QYKTKREEVK PVGDPGGNAT EVSVTTAMTT MSENKTKEYK
210 220 230 240 250
IVGLYSDGIN VLGLIIFCLV FGLVIGKMGE KGQILVDFFN ALSDATMKIV
260 270 280 290 300
QIIMCYMPIG ILFLIAGKII EVEDWEIFRK LGLYMATVLS GLAIHSLIVL
310 320 330 340 350
PLLYFIVVRK NPFRFALGMA QALLTALMIS SSSATLPVTF RCAEEKNQVD
360 370 380 390 400
KRITRFVLPV GATINMDGTA LYEAVAAVFI AQLNGLDLSI GQIVTISITA
410 420 430 440 450
TAASIGAAGV PQAGLVTMVI VLSAVGLPAE DVTLIIAVDW LLDRFRTMVN
460 470 480 490 500
VLGDAFGTGI VEKLSKKELE QMDVSSEVNI VNPFALEPTT LDNEDSDTKK
510 520
SYVNGGFAVD KSDTISFTQT SQF
Length:523
Mass (Da):56,694
Last modified:July 27, 2011 - v2
Checksum:i46F35480465E5A82
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti121 – 1211T → A in AAB71742 (Ref. 2) Curated
Sequence conflicti139 – 1391M → I in BAA07855 (PubMed:7766664).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D43797 mRNA. Translation: BAA07855.1.
U73521 mRNA. Translation: AAB80694.1.
U75217 Genomic DNA. Translation: AAB71742.1.
CH466534 Genomic DNA. Translation: EDL41660.1.
BC065099 mRNA. Translation: AAH65099.1.
CCDSiCCDS29727.1.
PIRiS55677.
RefSeqiNP_033225.1. NM_009199.2.
UniGeneiMm.246670.

Genome annotation databases

EnsembliENSMUST00000025875; ENSMUSP00000025875; ENSMUSG00000024935.
GeneIDi20510.
KEGGimmu:20510.
UCSCiuc008hcl.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D43797 mRNA. Translation: BAA07855.1.
U73521 mRNA. Translation: AAB80694.1.
U75217 Genomic DNA. Translation: AAB71742.1.
CH466534 Genomic DNA. Translation: EDL41660.1.
BC065099 mRNA. Translation: AAH65099.1.
CCDSiCCDS29727.1.
PIRiS55677.
RefSeqiNP_033225.1. NM_009199.2.
UniGeneiMm.246670.

3D structure databases

ProteinModelPortaliP51906.
SMRiP51906. Positions 28-119, 211-463.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP51906. 1 interaction.
MINTiMINT-2839452.
STRINGi10090.ENSMUSP00000025875.

PTM databases

PhosphoSiteiP51906.

Proteomic databases

MaxQBiP51906.
PaxDbiP51906.
PRIDEiP51906.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025875; ENSMUSP00000025875; ENSMUSG00000024935.
GeneIDi20510.
KEGGimmu:20510.
UCSCiuc008hcl.1. mouse.

Organism-specific databases

CTDi6505.
MGIiMGI:105083. Slc1a1.

Phylogenomic databases

eggNOGiCOG1301.
GeneTreeiENSGT00760000119117.
HOGENOMiHOG000208776.
HOVERGENiHBG000080.
InParanoidiP51906.
KOiK05612.
OMAiVSAVGWH.
OrthoDBiEOG7RV9G2.
TreeFamiTF315206.

Enzyme and pathway databases

ReactomeiREACT_277017. Transport of inorganic cations/anions and amino acids/oligopeptides.
REACT_287745. Glutamate Neurotransmitter Release Cycle.

Miscellaneous databases

ChiTaRSiSlc1a1. mouse.
NextBioi298699.
PROiP51906.
SOURCEiSearch...

Gene expression databases

BgeeiP51906.
ExpressionAtlasiP51906. baseline and differential.
GenevisibleiP51906. MM.

Family and domain databases

Gene3Di1.10.3860.10. 2 hits.
InterProiIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 1 hit.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 2 hits.
PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of two glutamate transporter subtypes from mouse brain."
    Mukainaka Y., Tanaka K., Hagiwara T., Wada K.
    Biochim. Biophys. Acta 1244:233-237(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: JCL:ICR.
    Tissue: Cerebellum.
  2. "Molecular cloning of a mouse glutamate transporter."
    Peng J.-B., Guo L.-H.
    Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Tissue: Brain.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  5. "Molecular cloning, gene structure, expression profile and functional characterization of the mouse glutamate transporter (EAAT3) interacting protein GTRAP3-18."
    Butchbach M.E.R., Lai L., Lin C.-L.G.
    Gene 292:81-90(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ARL6IP5.
    Strain: C57BL/6J.
    Tissue: Brain.

Entry informationi

Entry nameiEAA3_MOUSE
AccessioniPrimary (citable) accession number: P51906
Secondary accession number(s): O35869, O35875, Q6P1F7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 27, 2011
Last modified: June 24, 2015
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.