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P51892 (DNLI1_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA ligase 1
EC=6.5.1.1
Alternative name(s):
DNA ligase I
Polydeoxyribonucleotide synthase [ATP] 1
Gene names
Name:lig1
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length1070 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

Cofactor

Magnesium By similarity.

Subcellular location

Nucleus.

Sequence similarities

Belongs to the ATP-dependent DNA ligase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10701070DNA ligase 1
PRO_0000059573

Regions

Region602 – 61110Interaction with target DNA By similarity
Region795 – 7973Interaction with target DNA By similarity

Sites

Active site7211N6-AMP-lysine intermediate By similarity
Metal binding7741Magnesium 1 By similarity
Metal binding8731Magnesium 2 By similarity
Binding site7191ATP By similarity
Binding site7261ATP By similarity
Binding site7421ATP By similarity
Binding site8781ATP By similarity
Binding site8911ATP By similarity
Binding site8971ATP By similarity
Site4581Interaction with target DNA By similarity
Site7431Interaction with target DNA By similarity
Site9231Interaction with target DNA By similarity
Site9481Interaction with target DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
P51892 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 065D6E5B8C6E4E52

FASTA1,070120,234
        10         20         30         40         50         60 
MQRTIKSFFQ PKLGAEVKTK EEKVKNDVQK EKEEPEKSVP ERPLKERNGR ALCGDTESPV 

        70         80         90        100        110        120 
KRVSKKSARV LESDSEEEEE NSKVQATPEK MNNESDPKND VKETPPSARK ETPPSARKET 

       130        140        150        160        170        180 
PPSARKETPQ SARKETPPSA RKDAKSEGAS EMNTSQDFVS PCSSKSIDSP LCSDSPGISP 

       190        200        210        220        230        240 
SGIPKRKTAR KQLPKRKLEI SPSESNPPKD DFDVKGAVKR QKKEAEGDIQ QEEPMETSHN 

       250        260        270        280        290        300 
ISMEEECPIK KETNPDVVQE TISNADDPNI ELVDQKDVVS GEKQSEPKCQ EQPQPKLTSP 

       310        320        330        340        350        360 
TVEPKASKGK ARKKNSPLPK KVKFSDKVSF KDNDSEEKSN EEKSDEEPQK KADSAKPVKQ 

       370        380        390        400        410        420 
ISSFFAPRKP AIKTEKREEN LNEKNVTETS LEESPKPKKN VGSFFGASKQ EATEEQTEYN 

       430        440        450        460        470        480 
PSKSKYHPID DACWCNGQKV PYLAVARTFE RIEEESARLK NVETLSNFLR SVIALTPEDL 

       490        500        510        520        530        540 
LPCIYLCLNR LGPAYEGLEL GIGETILMKA VAQATGRQLE KIKAEAQEKG DLGLVAESSR 

       550        560        570        580        590        600 
SNQRTMFTPP KLMASGVFGK LKDIARMTGN ASMNKKIDII KGLFVACRHS EARYIARSLG 

       610        620        630        640        650        660 
GKLRIGLAEQ SVLSSIAQAV CLTPPGRDAP PTVMDAGKGM SADARKSWIE EKAMILKQTF 

       670        680        690        700        710        720 
CELPNYDAII PILLEHGIDD LPKHCRLTPG IPLKPMLAHP TKGIGEVLKR FDEAAFTCEY 

       730        740        750        760        770        780 
KYDGERAQIH ILENGEVHVY SRNQENNTTK YPDIISRIPK IKKESVKSCI LDTEAVAGDA 

       790        800        810        820        830        840 
EKKQIQPFQV LTTRKRKDVD ASEIKVQVCV YAFDMLYLNG ESLVKEPFAK RRQLLRDSFL 

       850        860        870        880        890        900 
ETEGQFMFAT YMDKSNTDEI SEFLDQSIKD SCEGLMVKTL EQDATYEIAK RSHNWLKLKK 

       910        920        930        940        950        960 
DYLEGVGDTL DLVVIGAYLG KGKRTGIYGG FLLASYDEES EEYQTICKIG TGFTDDDLEK 

       970        980        990       1000       1010       1020 
HYNFLKDHVI ENPRSYYRWD SATEPDHWFD PVQVWEVKCA DLSISPVHKA ALGLVEDQKG 

      1030       1040       1050       1060       1070 
ISLRFPRFLR IRDDKKPEES TNSFQVADLY RKQQQIQNTS STEKAEEDFY

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References

[1]"The cloning and characterization of a cDNA encoding Xenopus laevis DNA ligase I."
Lepetit D., Thiebaud P., Aoufouchi S., Prigent C., Guesne R., Theze N.
Gene 172:273-277(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L43496 mRNA. Translation: AAB37754.1.
PIRJC4852.
RefSeqNP_001081653.1. NM_001088184.1.
UniGeneXl.78.

3D structure databases

ProteinModelPortalP51892.
SMRP51892. Positions 419-1053.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID397978.
KEGGxla:397978.

Organism-specific databases

CTD3978.
XenbaseXB-GENE-1009783. lig1.

Phylogenomic databases

HOVERGENHBG005514.
KOK10747.

Family and domain databases

Gene3D1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
InterProIPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
SSF117018. SSF117018. 1 hit.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLI1_XENLA
AccessionPrimary (citable) accession number: P51892
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 3, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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