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Protein

DNA ligase 1

Gene

lig1

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).PROSITE-ProRule annotation

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei458 – 4581Interaction with target DNABy similarity
Binding sitei719 – 7191ATPBy similarity
Active sitei721 – 7211N6-AMP-lysine intermediatePROSITE-ProRule annotation
Binding sitei726 – 7261ATPBy similarity
Binding sitei742 – 7421ATPBy similarity
Sitei743 – 7431Interaction with target DNABy similarity
Metal bindingi774 – 7741Magnesium 1By similarity
Metal bindingi873 – 8731Magnesium 2By similarity
Binding sitei878 – 8781ATPBy similarity
Binding sitei891 – 8911ATPBy similarity
Binding sitei897 – 8971ATPBy similarity
Sitei923 – 9231Interaction with target DNABy similarity
Sitei948 – 9481Interaction with target DNABy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: InterPro
  3. DNA ligase (ATP) activity Source: UniProtKB-EC
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. DNA biosynthetic process Source: InterPro
  4. DNA ligation involved in DNA repair Source: InterPro
  5. DNA recombination Source: UniProtKB-KW
  6. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligase 1 (EC:6.5.1.1)
Alternative name(s):
DNA ligase I
Polydeoxyribonucleotide synthase [ATP] 1
Gene namesi
Name:lig1
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-1009783. lig1.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10701070DNA ligase 1PRO_0000059573Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP51892.
SMRiP51892. Positions 419-1053.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni602 – 61110Interaction with target DNABy similarity
Regioni795 – 7973Interaction with target DNABy similarity

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.Curated

Phylogenomic databases

HOVERGENiHBG005514.
KOiK10747.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
InterProiIPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P51892-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQRTIKSFFQ PKLGAEVKTK EEKVKNDVQK EKEEPEKSVP ERPLKERNGR
60 70 80 90 100
ALCGDTESPV KRVSKKSARV LESDSEEEEE NSKVQATPEK MNNESDPKND
110 120 130 140 150
VKETPPSARK ETPPSARKET PPSARKETPQ SARKETPPSA RKDAKSEGAS
160 170 180 190 200
EMNTSQDFVS PCSSKSIDSP LCSDSPGISP SGIPKRKTAR KQLPKRKLEI
210 220 230 240 250
SPSESNPPKD DFDVKGAVKR QKKEAEGDIQ QEEPMETSHN ISMEEECPIK
260 270 280 290 300
KETNPDVVQE TISNADDPNI ELVDQKDVVS GEKQSEPKCQ EQPQPKLTSP
310 320 330 340 350
TVEPKASKGK ARKKNSPLPK KVKFSDKVSF KDNDSEEKSN EEKSDEEPQK
360 370 380 390 400
KADSAKPVKQ ISSFFAPRKP AIKTEKREEN LNEKNVTETS LEESPKPKKN
410 420 430 440 450
VGSFFGASKQ EATEEQTEYN PSKSKYHPID DACWCNGQKV PYLAVARTFE
460 470 480 490 500
RIEEESARLK NVETLSNFLR SVIALTPEDL LPCIYLCLNR LGPAYEGLEL
510 520 530 540 550
GIGETILMKA VAQATGRQLE KIKAEAQEKG DLGLVAESSR SNQRTMFTPP
560 570 580 590 600
KLMASGVFGK LKDIARMTGN ASMNKKIDII KGLFVACRHS EARYIARSLG
610 620 630 640 650
GKLRIGLAEQ SVLSSIAQAV CLTPPGRDAP PTVMDAGKGM SADARKSWIE
660 670 680 690 700
EKAMILKQTF CELPNYDAII PILLEHGIDD LPKHCRLTPG IPLKPMLAHP
710 720 730 740 750
TKGIGEVLKR FDEAAFTCEY KYDGERAQIH ILENGEVHVY SRNQENNTTK
760 770 780 790 800
YPDIISRIPK IKKESVKSCI LDTEAVAGDA EKKQIQPFQV LTTRKRKDVD
810 820 830 840 850
ASEIKVQVCV YAFDMLYLNG ESLVKEPFAK RRQLLRDSFL ETEGQFMFAT
860 870 880 890 900
YMDKSNTDEI SEFLDQSIKD SCEGLMVKTL EQDATYEIAK RSHNWLKLKK
910 920 930 940 950
DYLEGVGDTL DLVVIGAYLG KGKRTGIYGG FLLASYDEES EEYQTICKIG
960 970 980 990 1000
TGFTDDDLEK HYNFLKDHVI ENPRSYYRWD SATEPDHWFD PVQVWEVKCA
1010 1020 1030 1040 1050
DLSISPVHKA ALGLVEDQKG ISLRFPRFLR IRDDKKPEES TNSFQVADLY
1060 1070
RKQQQIQNTS STEKAEEDFY
Length:1,070
Mass (Da):120,234
Last modified:October 1, 1996 - v1
Checksum:i065D6E5B8C6E4E52
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43496 mRNA. Translation: AAB37754.1.
PIRiJC4852.
RefSeqiNP_001081653.1. NM_001088184.1.
UniGeneiXl.78.

Genome annotation databases

GeneIDi397978.
KEGGixla:397978.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43496 mRNA. Translation: AAB37754.1.
PIRiJC4852.
RefSeqiNP_001081653.1. NM_001088184.1.
UniGeneiXl.78.

3D structure databases

ProteinModelPortaliP51892.
SMRiP51892. Positions 419-1053.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi397978.
KEGGixla:397978.

Organism-specific databases

CTDi3978.
XenbaseiXB-GENE-1009783. lig1.

Phylogenomic databases

HOVERGENiHBG005514.
KOiK10747.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
InterProiIPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The cloning and characterization of a cDNA encoding Xenopus laevis DNA ligase I."
    Lepetit D., Thiebaud P., Aoufouchi S., Prigent C., Guesne R., Theze N.
    Gene 172:273-277(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiDNLI1_XENLA
AccessioniPrimary (citable) accession number: P51892
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 7, 2015
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.