P51888 (PRELP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Prolargin

Alternative name(s):
Proline-arginine-rich end leucine-rich repeat protein

Gene names

Name:	PRELP
Synonyms:	SLRR2A

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	382 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May anchor basement membranes to the underlying connective tissue By similarity.
Subunit structure	Binds the basement membrane heparan sulfate proteoglycan perlecan and triple helical collagens type I and type II By similarity.
Subcellular location	Secreted › extracellular space › extracellular matrix.
Tissue specificity	Connective tissue.
Domain	The basic N-terminal Arg/Pro-rich region binds heparin and heparan sulfate. Binds collagens type I and type II through its leucine-rich repeat domain By similarity.
Sequence similarities	Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily. Contains 12 LRR (leucine-rich) repeats.

Ontologies

Keywords
Cellular component	Extracellular matrix Secreted
Coding sequence diversity	Polymorphism
Domain	Leucine-rich repeat Repeat Signal
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Traceable author statement. Source: Reactome cell aging Inferred from electronic annotation. Source: Compara keratan sulfate biosynthetic process Traceable author statement. Source: Reactome keratan sulfate catabolic process Traceable author statement. Source: Reactome skeletal system development Traceable author statement Ref.1. Source: ProtInc
Cellular_component	Golgi lumen Traceable author statement. Source: Reactome lysosomal lumen Traceable author statement. Source: Reactome proteinaceous extracellular matrix Traceable author statement Ref.1. Source: ProtInc
Molecular_function	extracellular matrix structural constituent Traceable author statement Ref.1. Source: ProtInc heparin binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 382	362	Prolargin		PRO_0000032744
Regions
	Repeat	95 – 114	20	LRR 1
	Repeat	115 – 138	24	LRR 2
	Repeat	139 – 162	24	LRR 3
	Repeat	163 – 183	21	LRR 4
	Repeat	184 – 207	24	LRR 5
	Repeat	208 – 233	26	LRR 6
	Repeat	234 – 254	21	LRR 7
	Repeat	255 – 278	24	LRR 8
	Repeat	279 – 303	25	LRR 9
	Repeat	304 – 323	20	LRR 10
	Repeat	324 – 362	39	LRR 11
	Repeat	363 – 382	20	LRR 12
	Compositional bias	73 – 89	17	Cys-rich
	Compositional bias	197 – 202	6	Poly-Leu
Amino acid modifications
	Glycosylation	124	1	N-linked (GlcNAc...) Ref.7
	Glycosylation	289	1	N-linked (GlcNAc...) Potential
	Glycosylation	320	1	N-linked (GlcNAc...) Potential
	Glycosylation	327	1	N-linked (GlcNAc...) Ref.7
	Disulfide bond	332 ↔ 373		By similarity
Natural variations
	Natural variant	33	1	G → R. Corresponds to variant rs41313926 [ dbSNP | Ensembl ].		VAR_061804
	Natural variant	157	1	M → V. Corresponds to variant rs2233726 [ dbSNP | Ensembl ].		VAR_052012
	Natural variant	334	1	N → S. Corresponds to variant rs2233732 [ dbSNP | Ensembl ].		VAR_052013
	Natural variant	348	1	N → H. Corresponds to variant rs9439 [ dbSNP | Ensembl ].		VAR_011976

Sequences

	Sequence		Length	Mass (Da)
	P51888 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: A1C4E166B7515695	FASTA	382	43,810
	10 20 30 40 50 60 MRSPLCWLLP LLILASVAQG QPTRRPRPGT GPGRRPRPRP RPTPSFPQPD EPAEPTDLPP 70 80 90 100 110 120 PLPPGPPSIF PDCPRECYCP PDFPSALYCD SRNLRKVPVI PPRIHYLYLQ NNFITELPVE 130 140 150 160 170 180 SFQNATGLRW INLDNNRIRK IDQRVLEKLP GLVFLYMEKN QLEEVPSALP RNLEQLRLSQ 190 200 210 220 230 240 NHISRIPPGV FSKLENLLLL DLQHNRLSDG VFKPDTFHGL KNLMQLNLAH NILRKMPPRV 250 260 270 280 290 300 PTAIHQLYLD SNKIETIPNG YFKSFPNLAF IRLNYNKLTD RGLPKNSFNI SNLLVLHLSH 310 320 330 340 350 360 NRISSVPAIN NRLEHLYLNN NSIEKINGTQ ICPNDLVAFH DFSSDLENVP HLRYLRLDGN 370 380 YLKPPIPLDL MMCFRLLQSV VI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The primary structure of a basic leucine-rich repeat protein, PRELP, found in connective tissues." Bengtsson E., Neame P.J., Heinegaard D., Sommarin Y. J. Biol. Chem. 270:25639-25644(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]	"The gene organization, chromosome location, and expression of a 55-kDa matrix protein (PRELP) of human articular cartilage." Grover J., Chen X.-N., Korenberg J.R., Recklies A.D., Roughley P.J. Genomics 38:109-117(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pancreas and Spleen.
[7]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-124 AND ASN-327, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U29089 mRNA. Translation: AAC50230.1. U41344, U41343 Genomic DNA. Translation: AAC18782.1. CR542270 mRNA. Translation: CAG47066.1. AL391817 Genomic DNA. Translation: CAI17033.1. CH471067 Genomic DNA. Translation: EAW91481.1. BC032498 mRNA. Translation: AAH32498.1.
IPI	IPI00020987.
PIR	I39068.
RefSeq	NP_002716.1. NM_002725.3. NP_958505.1. NM_201348.1.
UniGene	Hs.632481.
3D structure databases
ProteinModelPortal	P51888.
ModBase	Search...
Protein-protein interaction databases
IntAct	P51888. 1 interaction.
STRING	9606.ENSP00000343924.
PTM databases
PhosphoSite	P51888.
Polymorphism databases
DMDM	1709586.
Proteomic databases
PaxDb	P51888.
PeptideAtlas	P51888.
PRIDE	P51888.
Protocols and materials databases
DNASU	5549.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000343110; ENSP00000343924; ENSG00000188783.
GeneID	5549.
KEGG	hsa:5549.
UCSC	uc001gzs.3. human.
Organism-specific databases
CTD	5549.
GeneCards	GC01P203444.
HGNC	HGNC:9357. PRELP.
MIM	601914. gene.
neXtProt	NX_P51888.
PharmGKB	PA33729.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG4886.
HOGENOM	HOG000234447.
HOVERGEN	HBG108061.
InParanoid	P51888.
KO	K08125.
OMA	NKLEHLY.
OrthoDB	EOG44F69H.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism. REACT_116125. Disease.
Gene expression databases
ArrayExpress	P51888.
Bgee	P51888.
CleanEx	HS_PRELP.
Genevestigator	P51888.
GermOnline	ENSG00000188783. Homo sapiens.
Family and domain databases
InterPro	IPR001611. Leu-rich_rpt. IPR000372. LRR-contain_N. IPR027216. Prolargin. [Graphical view]
PANTHER	PTHR24371:SF15. PTHR24371:SF15. 1 hit.
Pfam	PF00560. LRR_1. 1 hit. PF01462. LRRNT. 1 hit. [Graphical view]
SMART	SM00013. LRRNT. 1 hit. [Graphical view]
PROSITE	PS51450. LRR. 11 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	PRELP. human.
GenomeRNAi	5549.
NextBio	21502.
PMAP-CutDB	P51888.
SOURCE	Search...

Entry information

Entry name

PRELP_HUMAN

Accession

Primary (citable) accession number: P51888
Secondary accession number(s): Q6FG38

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 119 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.