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Protein

ADP/ATP translocase 2

Gene

Slc25a5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the exchange of cytoplasmic ADP with mitochondrial ATP across the mitochondrial inner membrane. As part of the mitotic spindle-associated MMXD complex it may play a role in chromosome segregation (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei80 – 801NucleotideBy similarity

GO - Molecular functioni

  1. poly(A) RNA binding Source: MGI
  2. transporter activity Source: InterPro

GO - Biological processi

  1. chromosome segregation Source: UniProtKB-KW
  2. negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway Source: UniProtKB
  3. positive regulation of cell proliferation Source: UniProtKB
  4. transmembrane transport Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Chromosome partition, Transport

Enzyme and pathway databases

ReactomeiREACT_263148. Regulation of insulin secretion.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP/ATP translocase 2
Alternative name(s):
ADP,ATP carrier protein 2
Adenine nucleotide translocator 2
Short name:
ANT 2
Solute carrier family 25 member 5
Cleaved into the following chain:
Gene namesi
Name:Slc25a5
Synonyms:Ant2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:1353496. Slc25a5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei5 – 3935Helical; Name=1By similarityAdd
BLAST
Transmembranei75 – 10026Helical; Name=2By similarityAdd
BLAST
Transmembranei109 – 14335Helical; Name=3By similarityAdd
BLAST
Transmembranei176 – 20227Helical; Name=4By similarityAdd
BLAST
Transmembranei207 – 24135Helical; Name=5By similarityAdd
BLAST
Transmembranei273 – 29826Helical; Name=6By similarityAdd
BLAST

GO - Cellular componenti

  1. extracellular vesicular exosome Source: MGI
  2. integral component of membrane Source: UniProtKB-KW
  3. membrane Source: MGI
  4. mitochondrial inner membrane Source: MGI
  5. mitochondrial nucleoid Source: MGI
  6. mitochondrion Source: MGI
  7. MMXD complex Source: UniProtKB
  8. myelin sheath Source: UniProtKB
  9. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 298298ADP/ATP translocase 2PRO_0000423221Add
BLAST
Initiator methioninei1 – 11Removed; alternate1 Publication
Chaini2 – 298297ADP/ATP translocase 2, N-terminally processedPRO_0000090580Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei2 – 21N-acetylthreonine; in ADP/ATP translocase 2, N-terminally processed1 Publication
Modified residuei23 – 231N6-acetyllysine; alternateCurated
Modified residuei23 – 231N6-malonyllysine; alternateBy similarity
Modified residuei43 – 431N6-succinyllysine1 Publication
Modified residuei52 – 521N6,N6-dimethyllysine; alternateBy similarity
Modified residuei52 – 521N6-methyllysine; alternateBy similarity
Modified residuei92 – 921N6-acetyllysine; alternateCurated
Modified residuei92 – 921N6-malonyllysine; alternateBy similarity
Modified residuei96 – 961N6-malonyllysineBy similarity
Modified residuei105 – 1051N6-acetyllysine; alternate2 Publications
Modified residuei105 – 1051N6-succinyllysine; alternate1 Publication
Modified residuei147 – 1471N6-acetyllysine; alternate1 Publication
Modified residuei147 – 1471N6-malonyllysine; alternateBy similarity
Modified residuei147 – 1471N6-succinyllysine; alternate1 Publication
Modified residuei155 – 1551N6-acetyllysine; alternate1 Publication
Modified residuei155 – 1551N6-succinyllysine; alternate1 Publication
Modified residuei163 – 1631N6-acetyllysine1 Publication
Modified residuei166 – 1661N6-acetyllysine1 Publication
Modified residuei268 – 2681N6-acetyllysine; alternate1 Publication
Modified residuei268 – 2681N6-succinyllysine; alternate1 Publication

Keywords - PTMi

Acetylation, Methylation

Proteomic databases

MaxQBiP51881.
PaxDbiP51881.
PRIDEiP51881.

PTM databases

PhosphoSiteiP51881.

Expressioni

Gene expression databases

BgeeiP51881.
ExpressionAtlasiP51881. baseline and differential.
GenevestigatoriP51881.

Interactioni

Subunit structurei

Homodimer. Component of the MMXD complex, which includes CIAO1, ERCC2, FAM96B, MMS19 and SLC25A5 (By similarity).By similarity

Protein-protein interaction databases

BioGridi198106. 12 interactions.
DIPiDIP-31399N.
IntActiP51881. 11 interactions.
MINTiMINT-1839032.

Structurei

3D structure databases

ProteinModelPortaliP51881.
SMRiP51881. Positions 2-294.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati6 – 9893Solcar 1Add
BLAST
Repeati111 – 20191Solcar 2Add
BLAST
Repeati212 – 29786Solcar 3Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi235 – 2406Substrate recognitionBy similarity

Sequence similaritiesi

Contains 3 Solcar repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG238123.
HOGENOMiHOG000165727.
HOVERGENiHBG108348.
InParanoidiP51881.
KOiK05863.
OMAiNETAVSF.
OrthoDBiEOG7T1RBR.
PhylomeDBiP51881.
TreeFamiTF300743.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR002113. Aden_trnslctor.
IPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00927. ADPTRNSLCASE.
PR00926. MITOCARRIER.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P51881-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDAAVSFAK DFLAGGVAAA ISKTAVAPIE RVKLLLQVQH ASKQITADKQ
60 70 80 90 100
YKGIIDCVVR IPKEQGVLSF WRGNLANVIR YFPTQALNFA FKDKYKQIFL
110 120 130 140 150
GGVDKRTQFW RYFAGNLASG GAAGATSLCF VYPLDFARTR LAADVGKAGA
160 170 180 190 200
EREFKGLGDC LVKIYKSDGI KGLYQGFNVS VQGIIIYRAA YFGIYDTAKG
210 220 230 240 250
MLPDPKNTHI FISWMIAQSV TAVAGLTSYP FDTVRRRMMM QSGRKGTDIM
260 270 280 290
YTGTLDCWRK IARDEGSKAF FKGAWSNVLR GMGGAFVLVL YDEIKKYT
Length:298
Mass (Da):32,931
Last modified:January 23, 2007 - v3
Checksum:i0798E04B987EFE20
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27316 mRNA. Translation: AAC52838.1.
U10404 mRNA. Translation: AAA19009.1.
X70847 mRNA. Translation: CAA50196.1.
AF240003 Genomic DNA. Translation: AAF64471.1.
BC004570 mRNA. Translation: AAH04570.1.
BC086756 mRNA. Translation: AAH86756.1.
CCDSiCCDS30062.1.
PIRiS31814.
RefSeqiNP_031477.1. NM_007451.3.
UniGeneiMm.371544.

Genome annotation databases

EnsembliENSMUST00000016463; ENSMUSP00000016463; ENSMUSG00000016319.
GeneIDi11740.
KEGGimmu:11740.
UCSCiuc009sxs.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27316 mRNA. Translation: AAC52838.1.
U10404 mRNA. Translation: AAA19009.1.
X70847 mRNA. Translation: CAA50196.1.
AF240003 Genomic DNA. Translation: AAF64471.1.
BC004570 mRNA. Translation: AAH04570.1.
BC086756 mRNA. Translation: AAH86756.1.
CCDSiCCDS30062.1.
PIRiS31814.
RefSeqiNP_031477.1. NM_007451.3.
UniGeneiMm.371544.

3D structure databases

ProteinModelPortaliP51881.
SMRiP51881. Positions 2-294.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198106. 12 interactions.
DIPiDIP-31399N.
IntActiP51881. 11 interactions.
MINTiMINT-1839032.

PTM databases

PhosphoSiteiP51881.

Proteomic databases

MaxQBiP51881.
PaxDbiP51881.
PRIDEiP51881.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000016463; ENSMUSP00000016463; ENSMUSG00000016319.
GeneIDi11740.
KEGGimmu:11740.
UCSCiuc009sxs.1. mouse.

Organism-specific databases

CTDi292.
MGIiMGI:1353496. Slc25a5.

Phylogenomic databases

eggNOGiNOG238123.
HOGENOMiHOG000165727.
HOVERGENiHBG108348.
InParanoidiP51881.
KOiK05863.
OMAiNETAVSF.
OrthoDBiEOG7T1RBR.
PhylomeDBiP51881.
TreeFamiTF300743.

Enzyme and pathway databases

ReactomeiREACT_263148. Regulation of insulin secretion.

Miscellaneous databases

NextBioi279467.
PROiP51881.
SOURCEiSearch...

Gene expression databases

BgeeiP51881.
ExpressionAtlasiP51881. baseline and differential.
GenevestigatoriP51881.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR002113. Aden_trnslctor.
IPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00927. ADPTRNSLCASE.
PR00926. MITOCARRIER.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Rapid evolution of human pseudoautosomal genes and their mouse homologs."
    Ellison J.W., Li X., Francke U., Shapiro L.J.
    Mamm. Genome 7:25-30(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  2. Sheldon J.G.
    Thesis (1995), University of Cambridge, United Kingdom
    Cited for: NUCLEOTIDE SEQUENCE.
    Tissue: Skeletal muscle.
  3. Costet P., Laplace C.
    Submitted (FEB-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 129/Sv.
  4. Laplace C.
    Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  5. "Expression and sequence analysis of the mouse adenine nucleotide translocase 1 and 2 genes."
    Levy S.E., Chen Y.-S., Graham B.H., Wallace D.C.
    Gene 254:57-66(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6 and FVB/N.
    Tissue: Brain and Mammary gland.
  7. Bienvenut W.V.
    Submitted (JUL-2005) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-31; 34-43; 64-72; 81-92; 97-106 AND 189-199, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT THR-2, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Liver.
  8. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  9. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-105, SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-43; LYS-105; LYS-147; LYS-155 AND LYS-268, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast and Liver.
  10. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-105; LYS-147; LYS-155; LYS-163; LYS-166 AND LYS-268, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiADT2_MOUSE
AccessioniPrimary (citable) accession number: P51881
Secondary accession number(s): Q61311
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: February 4, 2015
This is version 136 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The transmembrane helices are not perpendicular to the plane of the membrane, but cross the membrane at an angle. Odd-numbered transmembrane helices exhibit a sharp kink, due to the presence of a conserved proline residue (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.