P51881 (ADT2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ADP/ATP translocase 2

Alternative name(s):
ADP,ATP carrier protein 2
Adenine nucleotide translocator 2
Short name=ANT 2
Solute carrier family 25 member 5

Gene names

Name:	Slc25a5
Synonyms:	Ant2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the exchange of cytoplasmic ADP with mitochondrial ATP across the mitochondrial inner membrane. As part of the mitotic spindle-associated MMXD complex it may play a role in chromosome segregation By similarity.
Subunit structure	Homodimer. Component of the MMXD complex, which includes CIAO1, ERCC2, FAM96B, MMS19 and SLC25A5 By similarity.
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein.
Miscellaneous	The transmembrane helices are not perpendicular to the plane of the membrane, but cross the membrane at an angle. Odd-numbered transmembrane helices exhibit a sharp kink, due to the presence of a conserved proline residue By similarity.
Sequence similarities	Belongs to the mitochondrial carrier family. Contains 3 Solcar repeats.

Ontologies

Keywords
Biological process	Chromosome partition Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Repeat Transmembrane Transmembrane helix
PTM	Acetylation Methylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	chromosome segregation Inferred from electronic annotation. Source: UniProtKB-KW transmembrane transport Inferred from electronic annotation. Source: InterPro
Cellular component	MMXD complex Inferred from sequence or structural similarity. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane Inferred from direct assay. Source: MGI
Molecular function	binding Inferred from electronic annotation. Source: InterPro transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.7
	Chain	2 – 298	297	ADP/ATP translocase 2		PRO_0000090580
Regions
	Transmembrane	5 – 39	35	Helical; Name=1; By similarity
	Transmembrane	75 – 100	26	Helical; Name=2; By similarity
	Transmembrane	109 – 143	35	Helical; Name=3; By similarity
	Transmembrane	176 – 202	27	Helical; Name=4; By similarity
	Transmembrane	207 – 241	35	Helical; Name=5; By similarity
	Transmembrane	273 – 298	26	Helical; Name=6; By similarity
	Repeat	6 – 98	93	Solcar 1
	Repeat	111 – 201	91	Solcar 2
	Repeat	212 – 297	86	Solcar 3
	Motif	235 – 240	6	Substrate recognition By similarity
Sites
	Binding site	80	1	Nucleotide By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylthreonine Ref.7
	Modified residue	23	1	N6-acetyllysine Ref.8
	Modified residue	42	1	Phosphoserine Ref.10
	Modified residue	52	1	N6,N6-dimethyllysine; alternate By similarity
	Modified residue	52	1	N6-methyllysine; alternate By similarity
	Modified residue	92	1	N6-acetyllysine Ref.8
	Modified residue	105	1	N6-acetyllysine By similarity
	Modified residue	155	1	N6-acetyllysine Ref.8
	Modified residue	163	1	N6-acetyllysine Ref.8
	Modified residue	166	1	N6-acetyllysine Ref.8
	Modified residue	191	1	Phosphotyrosine Ref.9
	Modified residue	195	1	Phosphotyrosine Ref.9
	Modified residue	199	1	N6-acetyllysine Ref.8

Sequences

	Sequence		Length	Mass (Da)
	P51881 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 0798E04B987EFE20	FASTA	298	32,931
	10 20 30 40 50 60 MTDAAVSFAK DFLAGGVAAA ISKTAVAPIE RVKLLLQVQH ASKQITADKQ YKGIIDCVVR 70 80 90 100 110 120 IPKEQGVLSF WRGNLANVIR YFPTQALNFA FKDKYKQIFL GGVDKRTQFW RYFAGNLASG 130 140 150 160 170 180 GAAGATSLCF VYPLDFARTR LAADVGKAGA EREFKGLGDC LVKIYKSDGI KGLYQGFNVS 190 200 210 220 230 240 VQGIIIYRAA YFGIYDTAKG MLPDPKNTHI FISWMIAQSV TAVAGLTSYP FDTVRRRMMM 250 260 270 280 290 QSGRKGTDIM YTGTLDCWRK IARDEGSKAF FKGAWSNVLR GMGGAFVLVL YDEIKKYT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rapid evolution of human pseudoautosomal genes and their mouse homologs." Ellison J.W., Li X., Francke U., Shapiro L.J. Mamm. Genome 7:25-30(1996) [PubMed: 8903724] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6. Tissue: Brain.
[2]	Sheldon J.G. Thesis (1995), University of Cambridge, United Kingdom Cited for: NUCLEOTIDE SEQUENCE. Tissue: Skeletal muscle.
[3]	Costet P., Laplace C. Submitted (FEB-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: 129/Sv.
[4]	Laplace C. Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[5]	"Expression and sequence analysis of the mouse adenine nucleotide translocase 1 and 2 genes." Levy S.E., Chen Y.-S., Graham B.H., Wallace D.C. Gene 254:57-66(2000) [PubMed: 10974536] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6 and FVB/N. Tissue: Brain and Mammary gland.
[7]	Bienvenut W.V. Submitted (JUL-2005) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-31; 34-43; 64-72; 81-92; 97-106 AND 189-199, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT THR-2, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Liver.
[8]	"Substrate and functional diversity of lysine acetylation revealed by a proteomics survey." Kim S.C., Sprung R., Chen Y., Xu Y., Ball H., Pei J., Cheng T., Kho Y., Xiao H., Xiao L., Grishin N.V., White M., Yang X.-J., Zhao Y. Mol. Cell 23:607-618(2006) [PubMed: 16916647] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-23; LYS-92; LYS-155; LYS-163; LYS-166 AND LYS-199, MASS SPECTROMETRY. Tissue: Liver.
[9]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed: 18034455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-191 AND TYR-195, MASS SPECTROMETRY. Tissue: Brain.
[10]	"Mitochondrial phosphoproteome revealed by an improved IMAC method and MS/MS/MS." Lee J., Xu Y., Chen Y., Sprung R., Kim S.C., Xie S., Zhao Y. Mol. Cell. Proteomics 6:669-676(2007) [PubMed: 17208939] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U27316 mRNA. Translation: AAC52838.1. U10404 mRNA. Translation: AAA19009.1. X70847 mRNA. Translation: CAA50196.1. AF240003 Genomic DNA. Translation: AAF64471.1. BC004570 mRNA. Translation: AAH04570.1. BC086756 mRNA. Translation: AAH86756.1.
IPI	IPI00127841.
PIR	S31814.
RefSeq	NP_031477.1. NM_007451.3.
UniGene	Mm.371544.
3D structure databases
ProteinModelPortal	P51881.
SMR	P51881. Positions 2-294.
ModBase	Search...
Protein-protein interaction databases
IntAct	P51881. 5 interactions.
STRING	P51881.
PTM databases
PhosphoSite	P51881.
Proteomic databases
PRIDE	P51881.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000016463; ENSMUSP00000016463; ENSMUSG00000016319.
GeneID	11740.
KEGG	mmu:11740.
Organism-specific databases
CTD	292.
MGI	MGI:1353496. Slc25a5.
Phylogenomic databases
eggNOG	roNOG06425.
HOGENOM	HBG610399.
HOVERGEN	HBG108348.
InParanoid	P51881.
OMA	GQAVKYD.
OrthoDB	EOG49CQ86.
PhylomeDB	P51881.
Gene expression databases
ArrayExpress	P51881.
Bgee	P51881.
Genevestigator	P51881.
GermOnline	ENSMUSG00000016319. Mus musculus.
Family and domain databases
InterPro	IPR002113. Aden_trnslctor. IPR002067. Mit_carrier. IPR018108. Mitochondrial_sb/sol_carrier. IPR023395. Mt_carrier_dom. [Graphical view]
Gene3D	G3DSA:1.50.40.10. G3DSA:1.50.40.10. 1 hit.
KO	K05863.
Pfam	PF00153. Mito_carr. 3 hits. [Graphical view]
PRINTS	PR00927. ADPTRNSLCASE. PR00926. MITOCARRIER.
SUPFAM	SSF103506. Mitoch_carrier. 1 hit.
PROSITE	PS50920. SOLCAR. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	279467.
SOURCE	Search...

Entry information

Entry name

ADT2_MOUSE

Accession

Primary (citable) accession number: P51881
Secondary accession number(s): Q61311

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 23, 2007
Last modified:	November 16, 2011
This is version 113 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program