ID   CASP5_HUMAN             Reviewed;         418 AA.
AC   P51878; Q1HBJ3;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   04-NOV-2008, sequence version 2.
DT   07-JUL-2009, entry version 83.
DE   RecName: Full=Caspase-5;
DE            Short=CASP-5;
DE            EC=3.4.22.58;
DE   AltName: Full=ICH-3 protease;
DE   AltName: Full=TY protease;
DE   AltName: Full=ICE(rel)-III;
DE   Contains:
DE     RecName: Full=Caspase-5 subunit p20;
DE   Contains:
DE     RecName: Full=Caspase-5 subunit p10;
DE   Flags: Precursor;
GN   Name=CASP5; Synonyms=ICH3;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND VARIANTS ALA-90 AND VAL-318.
RX   MEDLINE=95318183; PubMed=7797592; DOI=10.1074/jbc.270.26.15870;
RA   Munday N.A., Vaillancourt J.P., Ali A., Casano F.J., Miller D.K.,
RA   Molineaux S.M., Yamin T.-T., Yu V.L., Nicholson D.W.;
RT   "Molecular cloning and pro-apoptotic activity of ICErelII and
RT   ICErelIII, members of the ICE/CED-3 family of cysteine proteases.";
RL   J. Biol. Chem. 270:15870-15876(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS ASN-3; LEU-13; ARG-59;
RP   ALA-90; HIS-152; LEU-201; HIS-282; VAL-318; LYS-337 AND GLN-366.
RG   NIEHS SNPs program;
RL   Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16554811; DOI=10.1038/nature04632;
RA   Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K.,
RA   Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F.,
RA   Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E.,
RA   FitzGerald M.G., Jaffe D.B., LaButti K., Nicol R., Park H.-S.,
RA   Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W.,
RA   Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S.,
RA   Sakaki Y.;
RT   "Human chromosome 11 DNA sequence and analysis including novel gene
RT   identification.";
RL   Nature 440:497-500(2006).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 21-418, AND VARIANT ALA-90.
RC   TISSUE=Placenta, and Spleen;
RX   MEDLINE=96184899; PubMed=8617266;
RX   DOI=10.1111/j.1432-1033.1996.t01-1-00207.x;
RA   Faucheu C., Blanchet A.-M., Collard-Dutilleul V., Lalanne J.-L.,
RA   Diu-Hercend A.;
RT   "Identification of a cysteine protease closely related to interleukin-
RT   1 beta-converting enzyme.";
RL   Eur. J. Biochem. 236:207-213(1996).
CC   -!- FUNCTION: Mediator of programmed cell death (apoptosis).
CC   -!- CATALYTIC ACTIVITY: Strict requirement for Asp at the P1 position.
CC       It has a preferred cleavage sequence of Tyr-Val-Ala-Asp-|- but
CC       also cleaves at Asp-Glu-Val-Asp-|-.
CC   -!- SUBUNIT: Heterotetramer that consists of two anti-parallel
CC       arranged heterodimers, each one formed by a 20 kDa (p20) and a 10
CC       kDa (p10) subunits.
CC   -!- INTERACTION:
CC       Q9C000:NLRP1; NbExp=1; IntAct=EBI-1246700, EBI-1220518;
CC   -!- TISSUE SPECIFICITY: Expressed in barely detectable amounts in most
CC       tissues except brain, highest levels being found in lung, liver
CC       and skeletal muscle.
CC   -!- PTM: The two subunits are derived from the precursor sequence by
CC       an autocatalytic mechanism.
CC   -!- SIMILARITY: Belongs to the peptidase C14A family.
CC   -!- SIMILARITY: Contains 1 CARD domain.
CC   -!- WEB RESOURCE: Name=NIEHS-SNPs;
CC       URL="http://egp.gs.washington.edu/data/casp5/";
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; U28015; AAA75172.1; -; mRNA.
DR   EMBL; DQ508420; ABF47103.1; -; Genomic_DNA.
DR   EMBL; AP001153; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; X94993; CAA64450.1; ALT_INIT; mRNA.
DR   IPI; IPI00873979; -.
DR   PIR; B57511; B57511.
DR   UniGene; Hs.213327; -.
DR   HSSP; P29466; 1ICE.
DR   IntAct; P51878; 1.
DR   MEROPS; C14.008; -.
DR   Ensembl; ENSG00000137757; Homo sapiens.
DR   GeneCards; GC11M104370; -.
DR   HGNC; HGNC:1506; CASP5.
DR   MIM; 602665; gene.
DR   PharmGKB; PA26089; -.
DR   HOVERGEN; P51878; -.
DR   BRENDA; 3.4.22.58; 247.
DR   BindingDB; P51878; -.
DR   ArrayExpress; P51878; -.
DR   Bgee; P51878; -.
DR   CleanEx; HS_CASP5; -.
DR   GermOnline; ENSG00000137757; Homo sapiens.
DR   GO; GO:0005622; C:intracellular; IEA:InterPro.
DR   GO; GO:0004197; F:cysteine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   GO; GO:0006915; P:apoptosis; TAS:ProtInc.
DR   GO; GO:0006508; P:proteolysis; TAS:ProtInc.
DR   GO; GO:0042981; P:regulation of apoptosis; IEA:InterPro.
DR   InterPro; IPR001315; CARD.
DR   InterPro; IPR017350; Caspase_IL-1_beta.
DR   InterPro; IPR011029; DEATH-like.
DR   InterPro; IPR011600; Pept_C14_cat.
DR   InterPro; IPR001309; Pept_C14_ICE_p20.
DR   InterPro; IPR016129; Pept_C14_ICE_p20_AS.
DR   InterPro; IPR002138; Pept_C14_p10.
DR   InterPro; IPR002398; Pept_C14_p45.
DR   InterPro; IPR015917; Pept_C14_p45_core.
DR   Gene3D; G3DSA:1.10.533.10; DEATH_like; 1.
DR   PANTHER; PTHR10454; Pept_C14_p45; 1.
DR   Pfam; PF00619; CARD; 1.
DR   Pfam; PF00656; Peptidase_C14; 1.
DR   PIRSF; PIRSF038001; Caspase_ICE; 1.
DR   PRINTS; PR00376; IL1BCENZYME.
DR   SMART; SM00114; CARD; 1.
DR   SMART; SM00115; CASc; 1.
DR   PROSITE; PS50209; CARD; 1.
DR   PROSITE; PS01122; CASPASE_CYS; 1.
DR   PROSITE; PS01121; CASPASE_HIS; 1.
DR   PROSITE; PS50207; CASPASE_P10; 1.
DR   PROSITE; PS50208; CASPASE_P20; 1.
PE   1: Evidence at protein level;
KW   Apoptosis; Complete proteome; Hydrolase; Polymorphism; Protease;
KW   Thiol protease; Zymogen.
FT   PROPEP        1    120       Potential.
FT                                /FTId=PRO_0000004604.
FT   CHAIN       121    311       Caspase-5 subunit p20.
FT                                /FTId=PRO_0000004605.
FT   PROPEP      312    330       Potential.
FT                                /FTId=PRO_0000004606.
FT   CHAIN       331    418       Caspase-5 subunit p10.
FT                                /FTId=PRO_0000004607.
FT   DOMAIN       40    132       CARD.
FT   ACT_SITE    251    251       By similarity.
FT   ACT_SITE    299    299
FT   VARIANT       3      3       K -> N (in dbSNP:rs45483102).
FT                                /FTId=VAR_047216.
FT   VARIANT      10     10       L -> W (in dbSNP:rs1792778).
FT                                /FTId=VAR_047217.
FT   VARIANT      13     13       F -> L (in dbSNP:rs3181320).
FT                                /FTId=VAR_024403.
FT   VARIANT      59     59       L -> R.
FT                                /FTId=VAR_054480.
FT   VARIANT      90     90       T -> A (in dbSNP:rs507879).
FT                                /FTId=VAR_047218.
FT   VARIANT     152    152       R -> H (in dbSNP:rs3181179).
FT                                /FTId=VAR_024404.
FT   VARIANT     201    201       V -> L (in dbSNP:rs3181326).
FT                                /FTId=VAR_024405.
FT   VARIANT     282    282       R -> H.
FT                                /FTId=VAR_054481.
FT   VARIANT     318    318       L -> V (in dbSNP:rs523104).
FT                                /FTId=VAR_047219.
FT   VARIANT     337    337       E -> K (in dbSNP:rs45619739).
FT                                /FTId=VAR_047220.
FT   VARIANT     366    366       E -> Q.
FT                                /FTId=VAR_054482.
SQ   SEQUENCE   418 AA;  47858 MW;  018E53BCA9801368 CRC64;
     MFKGILQSGL DNFVINHMLK NNVAGQTSIQ TLVPNTDQKS TSVKKDNHKK KTVKMLEYLG
     KDVLHGVFNY LAKHDVLTLK EEEKKKYYDT KIEDKALILV DSLRKNRVAH QMFTQTLLNM
     DQKITSVKPL LQIEAGPPES AESTNILKLC PREEFLRLCK KNHDEIYPIK KREDRRRLAL
     IICNTKFDHL PARNGAHYDI VGMKRLLQGL GYTVVDEKNL TARDMESVLR AFAARPEHKS
     SDSTFLVLMS HGILEGICGT AHKKKKPDVL LYDTIFQIFN NRNCLSLKDK PKVIIVQACR
     GEKHGELWVR DSPASLALIS SQSSENLEAD SVCKIHEEKD FIAFCSSTPH NVSWRDRTRG
     SIFITELITC FQKYSCCCHL MEIFRKVQKS FEVPQAKAQM PTIERATLTR DFYLFPGN
//