Reviewed, UniProtKB/Swiss-Prot P51878 (CASP5_HUMAN)

Names and origin

Protein names

Recommended name:
    Caspase-5
      Short name=CASP-5
    EC=3.4.22.58
Alternative name(s):
    ICH-3 protease
    TY protease
    ICE(rel)-III
Cleaved into the following 2 chains:
    1- Recommended name:
            Caspase-5 subunit p20
    2- Recommended name:
            Caspase-5 subunit p10

Gene names

Name:	CASP5
Synonyms:	ICH3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	418 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Mediator of programmed cell death (apoptosis).
Catalytic activity	Strict requirement for Asp at the P1 position. It has a preferred cleavage sequence of Tyr-Val-Ala-Asp-|- but also cleaves at Asp-Glu-Val-Asp-|-.
Subunit structure	Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunits.
Tissue specificity	Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle.
Post-translational modification	The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
Sequence similarities	Belongs to the peptidase C14 family. Contains 1 CARD domain.

Ontologies

Keywords
Biological process	Apoptosis
Coding sequence diversity	Polymorphism
Molecular function	Hydrolase Protease Thiol protease
PTM	Zymogen
Gene Ontology (GO)
Biological process	proteolysis Ref.1 Traceable author statement. Source: ProtInc regulation of apoptosis Inferred from electronic annotation. Source: InterPro
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NLRP1	Q9C000	1	EBI-1246700,EBI-1220518

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 120	120	Potential		PRO_0000004604
	Chain	121 – 311	191	Caspase-5 subunit p20		PRO_0000004605
	Propeptide	312 – 330	19	Potential		PRO_0000004606
	Chain	331 – 418	88	Caspase-5 subunit p10		PRO_0000004607
Regions
	Domain	40 – 132	93	CARD
Sites
	Active site	251	1	By similarity
	Active site	299	1
Natural variations
	Natural variant	3	1	K → N: dbSNP rs45483102.		VAR_047216
	Natural variant	10	1	L → W: dbSNP rs1792778.		VAR_047217
	Natural variant	13	1	F → L: dbSNP rs3181320.		VAR_024403
	Natural variant	90	1	T → A: dbSNP rs507879.		VAR_047218
	Natural variant	152	1	R → H: dbSNP rs3181179.		VAR_024404
	Natural variant	201	1	V → L: dbSNP rs3181326.		VAR_024405
	Natural variant	318	1	L → V: dbSNP rs523104.		VAR_047219
	Natural variant	337	1	E → K: dbSNP rs45619739.		VAR_047220

Sequences

	Sequence		Length	Mass (Da)
	P51878-1 [UniParc]. Last modified November 4, 2008. Version 2. Checksum: 018E53BCA9801368	FASTA	418	47,858
	10 20 30 40 50 60 MFKGILQSGL DNFVINHMLK NNVAGQTSIQ TLVPNTDQKS TSVKKDNHKK KTVKMLEYLG 70 80 90 100 110 120 KDVLHGVFNY LAKHDVLTLK EEEKKKYYDT KIEDKALILV DSLRKNRVAH QMFTQTLLNM 130 140 150 160 170 180 DQKITSVKPL LQIEAGPPES AESTNILKLC PREEFLRLCK KNHDEIYPIK KREDRRRLAL 190 200 210 220 230 240 IICNTKFDHL PARNGAHYDI VGMKRLLQGL GYTVVDEKNL TARDMESVLR AFAARPEHKS 250 260 270 280 290 300 SDSTFLVLMS HGILEGICGT AHKKKKPDVL LYDTIFQIFN NRNCLSLKDK PKVIIVQACR 310 320 330 340 350 360 GEKHGELWVR DSPASLALIS SQSSENLEAD SVCKIHEEKD FIAFCSSTPH NVSWRDRTRG 370 380 390 400 410 SIFITELITC FQKYSCCCHL MEIFRKVQKS FEVPQAKAQM PTIERATLTR DFYLFPGN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and pro-apoptotic activity of ICErelII and ICErelIII, members of the ICE/CED-3 family of cysteine proteases." Munday N.A., Vaillancourt J.P., Ali A., Casano F.J., Miller D.K., Molineaux S.M., Yamin T.-T., Yu V.L., Nicholson D.W. J. Biol. Chem. 270:15870-15876(1995) [PubMed: 7797592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS ALA-90 AND VAL-318.
[2]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed: 16554811] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Identification of a cysteine protease closely related to interleukin-1 beta-converting enzyme." Faucheu C., Blanchet A.-M., Collard-Dutilleul V., Lalanne J.-L., Diu-Hercend A. Eur. J. Biochem. 236:207-213(1996) [PubMed: 8617266] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 21-418, VARIANT ALA-90. Tissue: Placenta and Spleen.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U28015 mRNA. Translation: AAA75172.1. AP001153 Genomic DNA. No translation available. X94993 mRNA. Translation: CAA64450.1. Different initiation.
PIR	B57511.
RefSeq	NP_004338.2.
UniGene	Hs.213327
3D structure databases
HSSP	HSSP built from PDB template 1ICE based on UniProtKB P29466.
ModBase	Search...
Protein-protein interaction databases
IntAct	P51878.
Protein family/group databases
MEROPS	C14.008.
PTM databases
PhosphoSite	P51878.
Genome annotation databases
Ensembl	ENSG00000137757. Homo sapiens. [Contig view]
GeneID	838.
Organism-specific databases
HGNC	HGNC:1506. CASP5.
MIM	602665. gene.
PharmGKB	PA26089.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOVERGEN	P51878.
Gene expression databases
ArrayExpress	P51878.
CleanEx	HS_CASP5.
GermOnline	ENSG00000137757. Homo sapiens.
Family and domain databases
InterPro	IPR001315. CARD. IPR017350. Caspase_IL-1_beta. IPR011029. DEATH_like. IPR011600. Pept_C14_cat. IPR001309. Pept_C14_ICE_p20. IPR016129. Pept_C14_ICE_p20_AS. IPR002138. Pept_C14_p10. IPR002398. Pept_C14_p45. IPR015917. Pept_C14_p45_core. [Graphical view]
Gene3D	G3DSA:1.10.533.10. DEATH_like. 1 hit.
PANTHER	PTHR10454. Pept_C14_p45. 1 hit.
Pfam	PF00619. CARD. 1 hit. PF00656. Peptidase_C14. 1 hit. [Graphical view]
PIRSF	PIRSF038001. Caspase_ICE. 1 hit.
PRINTS	PR00376. IL1BCENZYME.
SMART	SM00114. CARD. 1 hit. SM00115. CASc. 1 hit. [Graphical view]
PROSITE	PS50209. CARD. 1 hit. PS01122. CASPASE_CYS. 1 hit. PS01121. CASPASE_HIS. 1 hit. PS50207. CASPASE_P10. 1 hit. PS50208. CASPASE_P20. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
BindingDB	P51878.
SOURCE	Search...

Entry information

Entry name

CASP5_HUMAN

Accession

Primary (citable) accession number: P51878

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	November 4, 2008
Last modified:	November 25, 2008
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)