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Protein

Cytochrome P450 4F4

Gene

Cyp4f4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

heme1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei328 – 3281Heme (covalent; via 1 link)1 Publication
Metal bindingi468 – 4681Iron (heme axial ligand)1 Publication

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. heme binding Source: RGD
  3. iron ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 4F4 (EC:1.14.14.1)
Alternative name(s):
CYPIVF4
Gene namesi
Name:Cyp4f4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 7

Organism-specific databases

RGDi708363. Cyp4f4.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 522522Cytochrome P450 4F4PRO_0000051852Add
BLAST

Proteomic databases

PaxDbiP51869.
PRIDEiP51869.

Expressioni

Tissue specificityi

High expression in liver and kidney. Lower expression in brain.

Gene expression databases

GenevestigatoriP51869.

Interactioni

Protein-protein interaction databases

MINTiMINT-4570522.
STRINGi10116.ENSRNOP00000032609.

Structurei

3D structure databases

ProteinModelPortaliP51869.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118816.
HOGENOMiHOG000233833.
HOVERGENiHBG000182.
InParanoidiP51869.
KOiK00490.
OMAiGMITPTE.
OrthoDBiEOG7CNZFK.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P51869-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPQLDLSWLG LRLETSLPWL LLLLIGASWL LVRVLTQTYI FYRTYQHLCD
60 70 80 90 100
FPQPPKWNWF LGHLGMITPT EQGLKQVTKL VATYPQGFMT WLGPILPIIT
110 120 130 140 150
LCHPDVIRSV LSASASVALK EVIFYSFLKP WLGDGLLLSD GDKWSCHRRM
160 170 180 190 200
LTPAFHFNIL KPYVKIFNDS TNIMHAKWQD LASGGSARLD MFKNISLMTL
210 220 230 240 250
DSLQKCVFSF DSNCQEKPSE YISAILELSA LVAKRYQQLL LHTDSLYQLT
260 270 280 290 300
HNGRRFHKAC KLVHNFTDAV IQGRRRALPS QHEDDILKAK ARSKTLDFID
310 320 330 340 350
VLLLTKDEDG KELSDEDIRA EADTFMFEGH DTTASGLSWI LYNLARHPEY
360 370 380 390 400
QERCRQEVRE LLRDRESTEI EWDDLAQLPF LTMCIKESLR LHPPVTVISR
410 420 430 440 450
RCTQDIVLPD GRVIPKGVIC IINIFATHHN PTVWPDPEVY DPFRFDPENI
460 470 480 490 500
KDRSPLAFIP FSAGPRNCIG QTFAMNEMKV ALALTLLRFR VLPDDKEPRR
510 520
KPELILRAEG GLWLRVEPLS TQ
Length:522
Mass (Da):60,050
Last modified:October 1, 1996 - v1
Checksum:i193EA8E7CE8864D6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39206 mRNA. Translation: AAC52358.1.
PIRiJC4532.
RefSeqiNP_775146.1. NM_173123.1.
UniGeneiRn.10170.

Genome annotation databases

EnsembliENSRNOT00000041034; ENSRNOP00000039545; ENSRNOG00000032895.
GeneIDi286904.
KEGGirno:286904.
UCSCiRGD:708363. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39206 mRNA. Translation: AAC52358.1.
PIRiJC4532.
RefSeqiNP_775146.1. NM_173123.1.
UniGeneiRn.10170.

3D structure databases

ProteinModelPortaliP51869.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4570522.
STRINGi10116.ENSRNOP00000032609.

Proteomic databases

PaxDbiP51869.
PRIDEiP51869.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000041034; ENSRNOP00000039545; ENSRNOG00000032895.
GeneIDi286904.
KEGGirno:286904.
UCSCiRGD:708363. rat.

Organism-specific databases

CTDi286904.
RGDi708363. Cyp4f4.

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118816.
HOGENOMiHOG000233833.
HOVERGENiHBG000182.
InParanoidiP51869.
KOiK00490.
OMAiGMITPTE.
OrthoDBiEOG7CNZFK.

Miscellaneous databases

NextBioi625000.

Gene expression databases

GenevestigatoriP51869.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "cDNA cloning of three new forms of rat brain cytochrome P450 belonging to the CYP4F subfamily."
    Kawashima H., Strobel H.W.
    Biochem. Biophys. Res. Commun. 217:1137-1144(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "Covalent attachment of the heme prosthetic group in the CYP4F cytochrome P450 family."
    LeBrun L.A., Xu F., Kroetz D.L., Ortiz de Montellano P.R.
    Biochemistry 41:5931-5937(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: COVALENT HEME ATTACHMENT.

Entry informationi

Entry nameiCP4F4_RAT
AccessioniPrimary (citable) accession number: P51869
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 7, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.