Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Calsequestrin-2

Gene

Casq2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle (PubMed:8042990). Calcium ions are bound by clusters of acidic residues at the protein surface, especially at the interface between subunits. Can bind around 60 Ca2+ ions. Regulates the release of lumenal Ca2+ via the calcium release channel RYR2; this plays an important role in triggering muscle contraction. Plays a role in excitation-contraction coupling in the heart and in regulating the rate of heart beats (By similarity).By similarity1 Publication

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • protein homodimerization activity Source: BHF-UCL
  • protein kinase C binding Source: RGD

GO - Biological processi

  • negative regulation of ryanodine-sensitive calcium-release channel activity Source: BHF-UCL
  • regulation of cardiac muscle cell contraction Source: BHF-UCL
  • regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion Source: BHF-UCL

Keywordsi

Molecular functionMuscle protein
LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calsequestrin-2
Alternative name(s):
Calsequestrin, cardiac muscle isoform
Gene namesi
Name:Casq2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2276 Casq2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Sarcoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 191 PublicationAdd BLAST19
ChainiPRO_000000422120 – 413Calsequestrin-2Add BLAST394

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei282PhosphotyrosineCombined sources1
Glycosylationi335N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei398PhosphoserineCombined sources1
Modified residuei405PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation in the C-terminus, probably by CK2, moderately increases calcium buffering capacity.1 Publication
N-glycosylated.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP51868
PRIDEiP51868

PTM databases

iPTMnetiP51868
PhosphoSitePlusiP51868

Expressioni

Tissue specificityi

Detected in stomach and vas deferens (at protein level).1 Publication

Interactioni

Subunit structurei

Monomer, homodimer and homooligomer. Mostly monomeric in the absence of calcium. Forms higher oligomers in a calcium-dependent manner. Dimers associate to form tetramers, that then form linear homopolymer chains. Interacts with ASPH and TRDN (By similarity).By similarity

GO - Molecular functioni

  • protein homodimerization activity Source: BHF-UCL
  • protein kinase C binding Source: RGD

Protein-protein interaction databases

BioGridi247887, 1 interactor
IntActiP51868, 2 interactors
MINTiP51868
STRINGi10116.ENSRNOP00000021846

Structurei

3D structure databases

ProteinModelPortaliP51868
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi373 – 413Asp/Glu-rich (acidic)Add BLAST41

Sequence similaritiesi

Belongs to the calsequestrin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IGY5 Eukaryota
ENOG4111R2M LUCA
HOGENOMiHOG000049047
HOVERGENiHBG050805
InParanoidiP51868
PhylomeDBiP51868

Family and domain databases

InterProiView protein in InterPro
IPR001393 Calsequestrin
IPR018233 Calsequestrin_CS
IPR036249 Thioredoxin-like_sf
PANTHERiPTHR10033 PTHR10033, 1 hit
PfamiView protein in Pfam
PF01216 Calsequestrin, 1 hit
SUPFAMiSSF52833 SSF52833, 3 hits
PROSITEiView protein in PROSITE
PS00863 CALSEQUESTRIN_1, 1 hit
PS00864 CALSEQUESTRIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P51868-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRIYLLVVG LYLLSFSRAE EGLNFPTYDG KDRVVSLSEK NLKQVLKRYD
60 70 80 90 100
LLCLYYHEPV SSDKVAQKQF QLKEIVLELV AQVLEHKNIG FVMVDSRKEA
110 120 130 140 150
KLAKRLGFSE EGSLYVLKGG RTIEFDGEFA ADVLVEFLLD LIEDPVEIVN
160 170 180 190 200
NKLEVQAFER IEDQIKLLGF FKNEDSEYYK AFQEAAEHFQ PYIKFFATFD
210 220 230 240 250
KGVAKKLSLK MNEVGFYEPF MDEPSVIPNK PYTEEELVEF VKEHQRPTLR
260 270 280 290 300
PLRPEDMFET WEDDLNGIHI VAFAEKSDPD GYEFLEILKQ VARDNTDNPD
310 320 330 340 350
LSILWIDPDD FPLLVAYWEK TFKIDLFKPQ IGVVNVTDAD SVWMEIPDDD
360 370 380 390 400
DLPTAEELED WIEDVLSGKI NTEDDDNEDE DDDGDNDNDD DDDDDDNSDE
410
DNDDSDDDDD DDE
Length:413
Mass (Da):47,839
Last modified:October 25, 2005 - v2
Checksum:iF03B6C7C3EB19D80
GO

Sequence cautioni

The sequence AAH72547 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti50 – 52DLL → ARV in AAB58746 (Ref. 4) Curated3
Sequence conflicti208 – 211SLKM → FLEV in AAA75480 (Ref. 1) Curated4
Sequence conflicti251P → R in AAH72547 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33287 mRNA Translation: AAA75480.1
BC072547 mRNA Translation: AAH72547.1 Different initiation.
AF001334 Genomic DNA Translation: AAB58746.1
RefSeqiNP_058827.3, NM_017131.2
UniGeneiRn.10111

Genome annotation databases

GeneIDi29209
KEGGirno:29209
UCSCiRGD:2276 rat

Similar proteinsi

Entry informationi

Entry nameiCASQ2_RAT
AccessioniPrimary (citable) accession number: P51868
Secondary accession number(s): O09177, Q6IMX1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 25, 2005
Last modified: May 23, 2018
This is version 125 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health