P51867 (TNR6_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tumor necrosis factor receptor superfamily member 6

Alternative name(s):
Apo-1 antigen
Apoptosis-mediating surface antigen FAS
FASLG receptor
CD_antigen=CD95

Gene names

Name:	FAS
Synonyms:	APT1, TNFRSF6

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both By similarity.
Subunit structure	Binds DAXX and RIPK1. Interacts with HIPK3. Part of a complex containing HIPK3 and FADD. Interacts with BRE and FEM1B By similarity. Interacts with FADD By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein.
Domain	Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.
Sequence similarities	Contains 1 death domain. Contains 3 TNFR-Cys repeats.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Membrane
Domain	Repeat Signal Transmembrane Transmembrane helix
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	B cell mediated immunity Inferred from electronic annotation. Source: Compara activation-induced cell death of T cells Inferred from electronic annotation. Source: Compara cellular response to hyperoxia Inferred from electronic annotation. Source: Compara cellular response to lithium ion Inferred from electronic annotation. Source: Compara cellular response to mechanical stimulus Inferred from electronic annotation. Source: Compara extrinsic apoptotic signaling pathway via death domain receptors Inferred from electronic annotation. Source: Compara gene expression Inferred from electronic annotation. Source: Compara immunoglobulin production Inferred from electronic annotation. Source: Compara inflammatory cell apoptotic process Inferred from electronic annotation. Source: Compara negative regulation of B cell activation Inferred from electronic annotation. Source: Compara negative regulation of apoptotic process Inferred from electronic annotation. Source: Compara negative thymic T cell selection Inferred from electronic annotation. Source: Compara neuron apoptotic process Inferred from electronic annotation. Source: Compara positive regulation of apoptotic process Inferred from electronic annotation. Source: Compara positive regulation of necrotic cell death Inferred from electronic annotation. Source: Compara positive regulation of protein homooligomerization Inferred from electronic annotation. Source: Compara protein homooligomerization Inferred from electronic annotation. Source: Compara regulation of lymphocyte differentiation Inferred from electronic annotation. Source: Compara regulation of myeloid cell differentiation Inferred from electronic annotation. Source: Compara renal system process Inferred from electronic annotation. Source: Compara response to glucocorticoid stimulus Inferred from electronic annotation. Source: Compara response to toxin Inferred from electronic annotation. Source: Compara spleen development Inferred from electronic annotation. Source: Compara transformed cell apoptotic process Inferred from electronic annotation. Source: Compara
Cellular_component	CD95 death-inducing signaling complex Inferred from electronic annotation. Source: Compara cytoplasm Inferred from electronic annotation. Source: Compara external side of plasma membrane Inferred from electronic annotation. Source: Compara extracellular region Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane raft Inferred from electronic annotation. Source: Compara nucleus Inferred from electronic annotation. Source: Compara
Molecular_function	transmembrane signaling receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 323	301	Tumor necrosis factor receptor superfamily member 6		PRO_0000034561
Regions
	Topological domain	23 – 170	148	Extracellular Potential
	Transmembrane	171 – 188	18	Helical; Potential
	Topological domain	189 – 323	135	Cytoplasmic Potential
	Repeat	45 – 80	36	TNFR-Cys 1
	Repeat	81 – 124	44	TNFR-Cys 2
	Repeat	125 – 163	39	TNFR-Cys 3
	Domain	238 – 306	69	Death
	Region	204 – 305	102	Interaction with HIPK3 By similarity
Amino acid modifications
	Glycosylation	38	1	N-linked (GlcNAc...) Potential
	Glycosylation	115	1	N-linked (GlcNAc...) Potential
	Disulfide bond	45 ↔ 56		By similarity
	Disulfide bond	57 ↔ 70		By similarity
	Disulfide bond	60 ↔ 79		By similarity
	Disulfide bond	82 ↔ 98		By similarity
	Disulfide bond	101 ↔ 116		By similarity
	Disulfide bond	104 ↔ 124		By similarity
	Disulfide bond	126 ↔ 140		By similarity
	Disulfide bond	143 ↔ 154		By similarity
	Disulfide bond	146 ↔ 162		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P51867 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 4D88A90E9E1F4892	FASTA	323	36,445
	10 20 30 40 50 60 MSGIWVHLSL IFISVSGPLS KGENAHMAGI NSEGLKLNIT EANSCQEGLY REHQFCCQPC 70 80 90 100 110 120 PPGKRKNGDC KRDGDTPECV LCSEGNEYTD KSHHSDKCIR CSICDEEHGL EVEQNCTRTR 130 140 150 160 170 180 NTKCRCKSNF FCNSSPCEHC NPCTTCEHGI IEKCTPTSNT KCKGSRSHAN SLWALLILLI 190 200 210 220 230 240 PIVLIIYKVV KSRERNKKND YCNSAASNDE GRQLNLTDVD LGKYIPSIAE QMRITEVKEF 250 260 270 280 290 300 VRKNGMEEAK IDDIMHDNVH ETAEQKVQLL RNWYQSHGKK NAYCTLTKSL PKALAEKICD 310 320 IVMKDITNER ENANLQNENE NLV

References

[1]	"Cloning and characterization of the bovine Fas." Yoo J., Stone R.T., Beattie C.W. DNA Cell Biol. 15:227-234(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	U34794 mRNA. Translation: AAC48546.1.
IPI	IPI00707134.
RefSeq	NP_777087.1. NM_174662.2.
UniGene	Bt.4345.
3D structure databases
ProteinModelPortal	P51867.
SMR	P51867. Positions 214-323.
ModBase	Search...
Proteomic databases
PRIDE	P51867.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000014282; ENSBTAP00000014282; ENSBTAG00000010785.
GeneID	282488.
KEGG	bta:282488.
Organism-specific databases
CTD	355.
Phylogenomic databases
eggNOG	NOG45364.
GeneTree	ENSGT00530000063703.
HOGENOM	HOG000139681.
HOVERGEN	HBG004091.
InParanoid	P51867.
KO	K04390.
OMA	CVPCQEG.
OrthoDB	EOG4K6G53.
Family and domain databases
Gene3D	1.10.533.10. 1 hit.
InterPro	IPR011029. DEATH-like_dom. IPR000488. Death_domain. IPR008063. Fas_rcpt. IPR001368. TNFR/NGFR_Cys_rich_reg. [Graphical view]
Pfam	PF00531. Death. 1 hit. PF00020. TNFR_c6. 2 hits. [Graphical view]
PRINTS	PR01680. TNFACTORR6.
SMART	SM00005. DEATH. 1 hit. SM00208. TNFR. 3 hits. [Graphical view]
SUPFAM	SSF47986. DEATH_like. 1 hit.
PROSITE	PS50017. DEATH_DOMAIN. 1 hit. PS00652. TNFR_NGFR_1. 2 hits. PS50050. TNFR_NGFR_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	20806250.

Entry information

Entry name

TNR6_BOVIN

Accession

Primary (citable) accession number: P51867

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program