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Protein

Teratocarcinoma-derived growth factor

Gene

Tdgf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GPI-anchored cell membrane protein involved in Nodal signaling. Cell-associated Tdgf1 acts as a Nodal coreceptor in cis. Shedding of Tdgf1 by Tmem8a modulates Nodal signaling by allowing soluble Tdgf1 to act as a Nodal coreceptor on other cells. Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.By similarity

GO - Molecular functioni

  • coreceptor activity Source: UniProtKB
  • growth factor activity Source: MGI
  • nodal binding Source: UniProtKB
  • receptor binding Source: MGI
  • type I activin receptor binding Source: UniProtKB
  • Wnt-protein binding Source: UniProtKB

GO - Biological processi

  • activation of MAPK activity Source: MGI
  • anterior/posterior axis specification Source: MGI
  • anterior/posterior axis specification, embryo Source: MGI
  • BMP signaling pathway Source: MGI
  • canonical Wnt signaling pathway Source: MGI
  • cardiac muscle cell differentiation Source: MGI
  • cell differentiation Source: UniProtKB
  • cell migration involved in sprouting angiogenesis Source: MGI
  • cellular response to epidermal growth factor stimulus Source: MGI
  • cellular response to fibroblast growth factor stimulus Source: MGI
  • cellular response to hepatocyte growth factor stimulus Source: MGI
  • cellular response to interferon-gamma Source: MGI
  • cellular response to interleukin-6 Source: MGI
  • cellular response to tumor necrosis factor Source: MGI
  • gastrulation Source: MGI
  • heart development Source: UniProtKB
  • in utero embryonic development Source: MGI
  • morphogenesis of a branching structure Source: UniProtKB
  • negative regulation of apoptotic process Source: MGI
  • negative regulation of transforming growth factor beta receptor signaling pathway Source: MGI
  • nodal signaling pathway Source: UniProtKB
  • peptidyl-serine phosphorylation Source: MGI
  • positive regulation of cell-matrix adhesion Source: MGI
  • positive regulation of cell migration Source: UniProtKB
  • positive regulation of cell proliferation Source: MGI
  • positive regulation of endothelial cell migration Source: MGI
  • positive regulation of fibroblast proliferation Source: MGI
  • positive regulation of peptidyl-tyrosine phosphorylation Source: MGI
  • positive regulation of transcription by RNA polymerase II Source: MGI
  • regulation of cell migration Source: MGI
  • regulation of signal transduction Source: MGI
  • vasculogenesis Source: MGI

Keywordsi

Molecular functionGrowth factor

Names & Taxonomyi

Protein namesi
Recommended name:
Teratocarcinoma-derived growth factor
Alternative name(s):
Cripto growth factor
Epidermal growth factor-like Cripto protein
Gene namesi
Name:Tdgf1
Synonyms:Cripto
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:98658 Tdgf1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi107W → A: Unable to bind Cripto receptor. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
ChainiPRO_000000750418 – 134Teratocarcinoma-derived growth factorAdd BLAST117
PropeptideiPRO_0000395411135 – 171Removed in mature formBy similarityAdd BLAST37

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi63N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi66 ↔ 73PROSITE-ProRule annotation
Disulfide bondi67 ↔ 79PROSITE-ProRule annotation
Disulfide bondi81 ↔ 90PROSITE-ProRule annotation
Disulfide bondi99 ↔ 117PROSITE-ProRule annotation1 Publication
Disulfide bondi112 ↔ 133PROSITE-ProRule annotation1 Publication
Disulfide bondi115 ↔ 124PROSITE-ProRule annotation1 Publication
Lipidationi134GPI-anchor amidated aspartateBy similarity1

Post-translational modificationi

The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, it is processed by GPI processing phospholipase A2 (Tmem8a), removing an acyl-chain at the sn-2 position of GPI and releasing Tdgf1 as a lysophosphatidylinositol-bearing form, which is further cleaved by phospholipase D (Gpld1) into a soluble form.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiP51865
PRIDEiP51865

Expressioni

Tissue specificityi

Expressed at low level in specific organs of the adult animal such as spleen, heart, lung and brain. During gastrulation, expressed in the forming mesoderm. In later stages of the developing heart, expression is restricted to the truncus arteriosus.

Developmental stagei

First expressed prior to the onset of gastrulation (early streak stage), then continues throughout embryonic development.

Gene expression databases

CleanExiMM_TDGF1

Interactioni

Subunit structurei

Interacts with the activin type-1 receptor ACVR1B.By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

  • growth factor activity Source: MGI
  • nodal binding Source: UniProtKB
  • receptor binding Source: MGI
  • type I activin receptor binding Source: UniProtKB
  • Wnt-protein binding Source: UniProtKB

Protein-protein interaction databases

DIPiDIP-46063N
IntActiP51865, 4 interactors
STRINGi10090.ENSMUSP00000035075

Structurei

Secondary structure

1171
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi119 – 121Combined sources3
Beta strandi127 – 130Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2J5HNMR-A96-134[»]
ProteinModelPortaliP51865
SMRiP51865
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP51865

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini62 – 91EGF-likePROSITE-ProRule annotationAdd BLAST30

Sequence similaritiesi

Keywords - Domaini

EGF-like domain, Signal

Phylogenomic databases

eggNOGiKOG1217 Eukaryota
ENOG410XP6K LUCA
HOGENOMiHOG000145523
HOVERGENiHBG054705
InParanoidiP51865
PhylomeDBiP51865

Family and domain databases

InterProiView protein in InterPro
IPR017047 Cripto_growth_factor
IPR019011 Cryptic/Cripto_CFC-dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
PANTHERiPTHR14949:SF26 PTHR14949:SF26, 1 hit
PfamiView protein in Pfam
PF09443 CFC, 1 hit
PIRSFiPIRSF036301 Cripto_growth_factor, 1 hit
PROSITEiView protein in PROSITE
PS00022 EGF_1, 1 hit
PS50026 EGF_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P51865-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGYFSSSVVL LVAISSAFEF GPVAGRDLAI RDNSIWDQKE PAVRDRSFQF
60 70 80 90 100
VPSVGIQNSK SLNKTCCLNG GTCILGSFCA CPPSFYGRNC EHDVRKEHCG
110 120 130 140 150
SILHGTWLPK KCSLCRCWHG QLHCLPQTFL PGCDGHVMDQ DLKASRTPCQ
160 170
TPSVTTTFML AGACLFLDMK V
Length:171
Mass (Da):18,754
Last modified:October 1, 1996 - v1
Checksum:iC52051AEACDB5380
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M87321 mRNA Translation: AAA37459.1
X94083 Genomic DNA Translation: CAA63827.1
CCDSiCCDS40783.1
PIRiI49612
UniGeneiMm.5090

Similar proteinsi

Entry informationi

Entry nameiTDGF1_MOUSE
AccessioniPrimary (citable) accession number: P51865
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health