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Protein

RHO1 GDP-GTP exchange protein 2

Gene

ROM2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Stimulates the exchange of RHO1 GDP-bound form into GTP-bound form.

GO - Molecular functioni

  • phosphatidylinositol-4,5-bisphosphate binding Source: SGD
  • Rho guanyl-nucleotide exchange factor activity Source: SGD
  • signal transducer activity Source: SGD

GO - Biological processi

  • activation of GTPase activity Source: SGD
  • establishment of cell polarity Source: SGD
  • negative regulation of cAMP-mediated signaling Source: SGD
  • positive regulation of endocytosis Source: SGD
  • regulation of fungal-type cell wall organization Source: SGD
  • regulation of Rho protein signal transduction Source: InterPro
  • small GTPase mediated signal transduction Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Enzyme and pathway databases

BioCyciYEAST:G3O-32440-MONOMER.
ReactomeiR-SCE-193634. Axonal growth inhibition (RHOA activation).
R-SCE-193648. NRAGE signals death through JNK.
R-SCE-194840. Rho GTPase cycle.
R-SCE-416482. G alpha (12/13) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
RHO1 GDP-GTP exchange protein 2
Gene namesi
Name:ROM2
Ordered Locus Names:YLR371W
ORF Names:L8039.3
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR371W.
SGDiS000004363. ROM2.

Subcellular locationi

GO - Cellular componenti

  • cellular bud tip Source: SGD
  • incipient cellular bud site Source: SGD
  • mating projection tip Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000809692 – 1356RHO1 GDP-GTP exchange protein 2Add BLAST1355

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei76PhosphoserineCombined sources1
Modified residuei193PhosphoserineCombined sources1
Modified residuei223PhosphoserineCombined sources1
Modified residuei566PhosphoserineCombined sources1
Modified residuei628PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP51862.
PRIDEiP51862.

PTM databases

iPTMnetiP51862.

Interactioni

Protein-protein interaction databases

BioGridi31631. 266 interactors.
DIPiDIP-4663N.
IntActiP51862. 15 interactors.
MINTiMINT-562572.

Structurei

3D structure databases

ProteinModelPortaliP51862.
SMRiP51862.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini659 – 846DHPROSITE-ProRule annotationAdd BLAST188
Domaini1034 – 1336CNHPROSITE-ProRule annotationAdd BLAST303

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi252 – 265Poly-AsnAdd BLAST14
Compositional biasi329 – 336Poly-His8
Compositional biasi632 – 635Poly-Asp4

Sequence similaritiesi

Contains 1 CNH domain.PROSITE-ProRule annotation
Contains 1 DH (DBL-homology) domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00760000119193.
HOGENOMiHOG000142356.
InParanoidiP51862.
KOiK19842.
OMAiPNFIEIR.
OrthoDBiEOG092C0E8G.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.20.900.10. 1 hit.
InterProiIPR001180. CNH_dom.
IPR000591. DEP_dom.
IPR000219. DH-domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00780. CNH. 1 hit.
PF00610. DEP. 1 hit.
PF00621. RhoGEF. 1 hit.
[Graphical view]
SMARTiSM00036. CNH. 1 hit.
SM00049. DEP. 1 hit.
SM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF48065. SSF48065. 1 hit.
PROSITEiPS50219. CNH. 1 hit.
PS50010. DH_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P51862-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSETNVDSLG DRNDIYSQIF GVERRPDSFA TFDSDSHGDI SSQLLPNRIE
60 70 80 90 100
NIQNLNVLLS EDIANDIIIA KQRRRSGVEA AIDDSDIPNN EMKGKSSNYI
110 120 130 140 150
LSQQTNIKEV PDTQSLSSAD NTPVSSPKKA RDATSSHPIV HAKSMSHIYS
160 170 180 190 200
TSNSASRQAK HYNDHPLPPM SPRNEVYQKN KSTTAFVPKR KPSLPQLALA
210 220 230 240 250
GLKKQSSFST GSASTTPTQA RKSPLQGFGF FSRPSSKDLH EQHQHHQHIQ
260 270 280 290 300
HNNINNHNNN NTNNNGAHYQ VGSSNSNYPQ HSHSISSRSM SLNSSTLKNI
310 320 330 340 350
ASSFQSKTSN SRKATQKYDI TSNPFSDPHH HHHHHHSSNS HSSLNNVHGS
360 370 380 390 400
GNSSSVMGSS SNIGLGLKTR VSSTSLALKR YTSVSGTSLS SPRRSSMTPL
410 420 430 440 450
SASRPVMSAS SKKPQVYPAL LSRVATKFKS SIQLGEHKKD GLVYRDAFTG
460 470 480 490 500
QQAVDVICAI IRTSDRNLAL LFGRSLDAQK LFHDVVYEHR LRDSPHEVYE
510 520 530 540 550
FTDNSRFTGT GSTNAHDPLM LLPNSSSFNS GNHSYPNSGM VPSSSTSSLN
560 570 580 590 600
SDQATLTGSR LHMSSSLSQQ KNPAAIHNVN GVFTLLAECY SPTCTRDALC
610 620 630 640 650
YSISCPRRLE QQARLNLKPN GGLKRNISMA LDDDDEEKPS WTSSVSKEDW
660 670 680 690 700
ENLPKKEIKR QEAIYEVYIT EKNFVKSLEI TRDTFMKTLA ETNIISADIR
710 720 730 740 750
KNFIKHVFAH INDIYSVNRR FLKALTDRQR SSPVVRGIGD IVLRFIPFFE
760 770 780 790 800
PFVSYVASRP YAKYLIETQR SVNPYFARFD DDMMSSSLRH GIDSFLSQGV
810 820 830 840 850
SRPGRYMLLV KEIMKSTDPE KDKSDYEDLS KAMDALRDFM KRIDQASGAA
860 870 880 890 900
QDRHDVKLLK QKILFKNEYV NLGLNDERRK IKHEGILSRK ELSKSDGTVV
910 920 930 940 950
GDIQFYLLDN MLLFLKAKAV NKWHQHKVFQ RPIPLPLLFA CPGEDMPALR
960 970 980 990 1000
KYIGDHPDCS GTVIQPEYNT SNPKNAITFL YYGAKQRYQV TLYAAQYAGL
1010 1020 1030 1040 1050
QTLLEKIKQG QAAIISKTEM FNVTKMSDRF FDYTNKINSV TSCDGGRKLL
1060 1070 1080 1090 1100
IATNSGLYMS NIKRQQNKDH RHKSSAFFST PIQLVQRNNI TQIAVLEEFK
1110 1120 1130 1140 1150
SIILLIDKKL YSCPLSLIEA EGNGTSFFKK HHKELINHVS FFAEGDCNGK
1160 1170 1180 1190 1200
RLIVTAHSSS HSIKYFEHEH PLLAEKNGSG SGNKKSLKKK ITEVIFDSEP
1210 1220 1230 1240 1250
VSISFLKANL CIGCKKGFQI VSISQNAHES LLDPADTSLE FALRDTLKPM
1260 1270 1280 1290 1300
AIYRVGNMFL LCYTEFAFFV NNQGWRKKES HIIHWEGEPQ KFAIWYPYIL
1310 1320 1330 1340 1350
AFDSNFIEIR KIETGELIRC VLADKIRLLQ TSTQEILYCY EDYRGYDTVA

SLDFWG
Length:1,356
Mass (Da):152,596
Last modified:October 1, 1996 - v1
Checksum:i5FBC542114E7BC92
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19103 Genomic DNA. Translation: AAB67564.1.
BK006945 Genomic DNA. Translation: DAA09674.1.
PIRiS51389.
RefSeqiNP_013475.1. NM_001182260.1.

Genome annotation databases

EnsemblFungiiYLR371W; YLR371W; YLR371W.
GeneIDi851086.
KEGGisce:YLR371W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19103 Genomic DNA. Translation: AAB67564.1.
BK006945 Genomic DNA. Translation: DAA09674.1.
PIRiS51389.
RefSeqiNP_013475.1. NM_001182260.1.

3D structure databases

ProteinModelPortaliP51862.
SMRiP51862.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31631. 266 interactors.
DIPiDIP-4663N.
IntActiP51862. 15 interactors.
MINTiMINT-562572.

PTM databases

iPTMnetiP51862.

Proteomic databases

MaxQBiP51862.
PRIDEiP51862.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR371W; YLR371W; YLR371W.
GeneIDi851086.
KEGGisce:YLR371W.

Organism-specific databases

EuPathDBiFungiDB:YLR371W.
SGDiS000004363. ROM2.

Phylogenomic databases

GeneTreeiENSGT00760000119193.
HOGENOMiHOG000142356.
InParanoidiP51862.
KOiK19842.
OMAiPNFIEIR.
OrthoDBiEOG092C0E8G.

Enzyme and pathway databases

BioCyciYEAST:G3O-32440-MONOMER.
ReactomeiR-SCE-193634. Axonal growth inhibition (RHOA activation).
R-SCE-193648. NRAGE signals death through JNK.
R-SCE-194840. Rho GTPase cycle.
R-SCE-416482. G alpha (12/13) signalling events.

Miscellaneous databases

PROiP51862.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.20.900.10. 1 hit.
InterProiIPR001180. CNH_dom.
IPR000591. DEP_dom.
IPR000219. DH-domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00780. CNH. 1 hit.
PF00610. DEP. 1 hit.
PF00621. RhoGEF. 1 hit.
[Graphical view]
SMARTiSM00036. CNH. 1 hit.
SM00049. DEP. 1 hit.
SM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF48065. SSF48065. 1 hit.
PROSITEiPS50219. CNH. 1 hit.
PS50010. DH_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiROM2_YEAST
AccessioniPrimary (citable) accession number: P51862
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.