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P51855

- GSHB_MOUSE

UniProt

P51855 - GSHB_MOUSE

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Protein

Glutathione synthetase

Gene

Gss

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

Cofactori

Mg2+By similarityNote: Binds 1 Mg(2+) ion per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei125 – 1251SubstrateBy similarity
Metal bindingi144 – 1441MagnesiumBy similarity
Binding sitei144 – 1441ATPBy similarity
Metal bindingi146 – 1461MagnesiumBy similarity
Binding sitei220 – 2201SubstrateBy similarity
Binding sitei305 – 3051ATPBy similarity
Metal bindingi368 – 3681MagnesiumBy similarity
Binding sitei375 – 3751ATPBy similarity
Binding sitei425 – 4251ATPBy similarity
Binding sitei450 – 4501SubstrateBy similarity
Binding sitei452 – 4521ATPBy similarity
Binding sitei458 – 4581ATP; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi364 – 37310ATPBy similarity
Nucleotide bindingi398 – 4014ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. glutathione binding Source: UniProtKB
  3. glutathione synthase activity Source: MGI
  4. glycine binding Source: Ensembl
  5. magnesium ion binding Source: UniProtKB
  6. protein homodimerization activity Source: UniProtKB

GO - Biological processi

  1. aging Source: Ensembl
  2. response to amino acid Source: Ensembl
  3. response to cadmium ion Source: MGI
  4. response to nutrient levels Source: Ensembl
  5. response to tumor necrosis factor Source: Ensembl
  6. response to xenobiotic stimulus Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_210980. Glutathione synthesis and recycling.
SABIO-RKP51855.
UniPathwayiUPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetase (EC:6.3.2.3)
Short name:
GSH synthetase
Short name:
GSH-S
Alternative name(s):
Glutathione synthase
Gene namesi
Name:Gss
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:95852. Gss.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 474473Glutathione synthetasePRO_0000211262Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP51855.
PaxDbiP51855.
PRIDEiP51855.

PTM databases

PhosphoSiteiP51855.

Expressioni

Gene expression databases

BgeeiP51855.
CleanExiMM_GSS.
ExpressionAtlasiP51855. baseline and differential.
GenevestigatoriP51855.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiP51855. 2 interactions.
MINTiMINT-1854502.

Structurei

3D structure databases

ProteinModelPortaliP51855.
SMRiP51855. Positions 3-474.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni148 – 1514Substrate bindingBy similarity
Regioni214 – 2163Substrate bindingBy similarity
Regioni267 – 2704Substrate bindingBy similarity
Regioni461 – 4622Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the eukaryotic GSH synthase family.Curated

Phylogenomic databases

eggNOGiNOG329040.
HOGENOMiHOG000172641.
HOVERGENiHBG002458.
InParanoidiP51855.
KOiK01920.
OMAiGWLLRSK.
OrthoDBiEOG757CXD.
PhylomeDBiP51855.
TreeFamiTF105187.

Family and domain databases

Gene3Di1.10.1080.10. 2 hits.
3.30.1490.50. 1 hit.
3.30.1490.80. 2 hits.
3.40.50.1760. 1 hit.
InterProiIPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR11130. PTHR11130. 1 hit.
PfamiPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001558. GSHase. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P51855-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATSWGSILQ DEKQLEELAK QAIDRALAEG VLLRSAQHPS SSDVVTYAPF
60 70 80 90 100
TLFPSPVPSA LLEQAYAVQM DFNILVDAVS QNPAFLEQTL SSTIKKDDYT
110 120 130 140 150
ARLFDIYKQV LKEGIAQTVF LGLNRSDYMF QCGADGSKAL KQIEINTISA
160 170 180 190 200
SFGGLASRTP AVHRHVLNVL NKTKEASKIL SNNPSKGLAL GIAKAWELYG
210 220 230 240 250
SANAVVLLIA QEKERNIFDQ RAVENELLDR KIHVIRGRFE DVSERGSLDQ
260 270 280 290 300
NRRLFMDDQE VAVVYFRDGY MPSQYNSQNW EARLMLERSR AAKCPDIAIQ
310 320 330 340 350
LAGTKKVQQE LSRVGLLEAL LPGQPEAVAR LRATFAGLYS LDMGEEGDQA
360 370 380 390 400
IAEALAAPSH FVLKPQREGG GNNLYGEEMV QALEQLKDSE ERASYILMEK
410 420 430 440 450
IEPEPFRNCL LRPGSPAQVV QCISELGIFG VYVRQGTTLV MNKHVGHLLR
460 470
TKAVEHADGG VAAGVAVLDN PYPV
Length:474
Mass (Da):52,247
Last modified:October 1, 1996 - v1
Checksum:iE1302C5B2C28A43A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35456 mRNA. Translation: AAB09730.1.
BC003784 mRNA. Translation: AAH03784.1.
CCDSiCCDS16951.1.
PIRiS71322.
RefSeqiNP_032206.1. NM_008180.2.
XP_006498844.1. XM_006498781.1.
UniGeneiMm.252316.

Genome annotation databases

EnsembliENSMUST00000079691; ENSMUSP00000078630; ENSMUSG00000027610.
GeneIDi14854.
KEGGimmu:14854.
UCSCiuc008nkx.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35456 mRNA. Translation: AAB09730.1 .
BC003784 mRNA. Translation: AAH03784.1 .
CCDSi CCDS16951.1.
PIRi S71322.
RefSeqi NP_032206.1. NM_008180.2.
XP_006498844.1. XM_006498781.1.
UniGenei Mm.252316.

3D structure databases

ProteinModelPortali P51855.
SMRi P51855. Positions 3-474.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P51855. 2 interactions.
MINTi MINT-1854502.

PTM databases

PhosphoSitei P51855.

Proteomic databases

MaxQBi P51855.
PaxDbi P51855.
PRIDEi P51855.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000079691 ; ENSMUSP00000078630 ; ENSMUSG00000027610 .
GeneIDi 14854.
KEGGi mmu:14854.
UCSCi uc008nkx.1. mouse.

Organism-specific databases

CTDi 2937.
MGIi MGI:95852. Gss.

Phylogenomic databases

eggNOGi NOG329040.
HOGENOMi HOG000172641.
HOVERGENi HBG002458.
InParanoidi P51855.
KOi K01920.
OMAi GWLLRSK.
OrthoDBi EOG757CXD.
PhylomeDBi P51855.
TreeFami TF105187.

Enzyme and pathway databases

UniPathwayi UPA00142 ; UER00210 .
Reactomei REACT_210980. Glutathione synthesis and recycling.
SABIO-RK P51855.

Miscellaneous databases

NextBioi 287083.
PROi P51855.
SOURCEi Search...

Gene expression databases

Bgeei P51855.
CleanExi MM_GSS.
ExpressionAtlasi P51855. baseline and differential.
Genevestigatori P51855.

Family and domain databases

Gene3Di 1.10.1080.10. 2 hits.
3.30.1490.50. 1 hit.
3.30.1490.80. 2 hits.
3.40.50.1760. 1 hit.
InterProi IPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view ]
PANTHERi PTHR11130. PTHR11130. 1 hit.
Pfami PF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view ]
PIRSFi PIRSF001558. GSHase. 1 hit.
SUPFAMi SSF52440. SSF52440. 1 hit.
TIGRFAMsi TIGR01986. glut_syn_euk. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A single mouse glutathione synthetase gene encodes six mRNAs with different 5' ends."
    Shi Z.Z., Carter B.Z., Habib G.M., He X., Sazer S., Lebovitz R.M., Lieberman M.W.
    Arch. Biochem. Biophys. 331:215-224(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Kidney.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiGSHB_MOUSE
AccessioniPrimary (citable) accession number: P51855
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 26, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3