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P51855

- GSHB_MOUSE

UniProt

P51855 - GSHB_MOUSE

Protein

Glutathione synthetase

Gene

Gss

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei125 – 1251SubstrateBy similarity
    Metal bindingi144 – 1441MagnesiumBy similarity
    Binding sitei144 – 1441ATPBy similarity
    Metal bindingi146 – 1461MagnesiumBy similarity
    Binding sitei220 – 2201SubstrateBy similarity
    Binding sitei305 – 3051ATPBy similarity
    Metal bindingi368 – 3681MagnesiumBy similarity
    Binding sitei375 – 3751ATPBy similarity
    Binding sitei425 – 4251ATPBy similarity
    Binding sitei450 – 4501SubstrateBy similarity
    Binding sitei452 – 4521ATPBy similarity
    Binding sitei458 – 4581ATP; via carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi364 – 37310ATPBy similarity
    Nucleotide bindingi398 – 4014ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. glutathione binding Source: UniProtKB
    3. glutathione synthase activity Source: MGI
    4. glycine binding Source: Ensembl
    5. magnesium ion binding Source: UniProtKB
    6. protein homodimerization activity Source: UniProtKB

    GO - Biological processi

    1. aging Source: Ensembl
    2. response to amino acid Source: Ensembl
    3. response to cadmium ion Source: MGI
    4. response to nutrient levels Source: Ensembl
    5. response to tumor necrosis factor Source: Ensembl
    6. response to xenobiotic stimulus Source: Ensembl

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Glutathione biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_210980. Glutathione synthesis and recycling.
    SABIO-RKP51855.
    UniPathwayiUPA00142; UER00210.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione synthetase (EC:6.3.2.3)
    Short name:
    GSH synthetase
    Short name:
    GSH-S
    Alternative name(s):
    Glutathione synthase
    Gene namesi
    Name:Gss
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:95852. Gss.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 474473Glutathione synthetasePRO_0000211262Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP51855.
    PaxDbiP51855.
    PRIDEiP51855.

    PTM databases

    PhosphoSiteiP51855.

    Expressioni

    Gene expression databases

    BgeeiP51855.
    CleanExiMM_GSS.
    GenevestigatoriP51855.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    IntActiP51855. 2 interactions.
    MINTiMINT-1854502.

    Structurei

    3D structure databases

    ProteinModelPortaliP51855.
    SMRiP51855. Positions 3-474.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni148 – 1514Substrate bindingBy similarity
    Regioni214 – 2163Substrate bindingBy similarity
    Regioni267 – 2704Substrate bindingBy similarity
    Regioni461 – 4622Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the eukaryotic GSH synthase family.Curated

    Phylogenomic databases

    eggNOGiNOG329040.
    HOGENOMiHOG000172641.
    HOVERGENiHBG002458.
    InParanoidiP51855.
    KOiK01920.
    OMAiGWLLRSK.
    OrthoDBiEOG757CXD.
    PhylomeDBiP51855.
    TreeFamiTF105187.

    Family and domain databases

    Gene3Di1.10.1080.10. 2 hits.
    3.30.1490.50. 1 hit.
    3.30.1490.80. 2 hits.
    3.40.50.1760. 1 hit.
    InterProiIPR004887. Glutathione_synth_subst-bd_euk.
    IPR014042. Glutathione_synthase_a-hlx_euk.
    IPR014709. Glutathione_synthase_dom.
    IPR005615. Glutathione_synthase_euk.
    IPR014049. Glutathione_synthase_N_euk.
    IPR016185. PreATP-grasp_dom.
    [Graphical view]
    PANTHERiPTHR11130. PTHR11130. 1 hit.
    PfamiPF03917. GSH_synth_ATP. 1 hit.
    PF03199. GSH_synthase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001558. GSHase. 1 hit.
    SUPFAMiSSF52440. SSF52440. 1 hit.
    TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P51855-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATSWGSILQ DEKQLEELAK QAIDRALAEG VLLRSAQHPS SSDVVTYAPF    50
    TLFPSPVPSA LLEQAYAVQM DFNILVDAVS QNPAFLEQTL SSTIKKDDYT 100
    ARLFDIYKQV LKEGIAQTVF LGLNRSDYMF QCGADGSKAL KQIEINTISA 150
    SFGGLASRTP AVHRHVLNVL NKTKEASKIL SNNPSKGLAL GIAKAWELYG 200
    SANAVVLLIA QEKERNIFDQ RAVENELLDR KIHVIRGRFE DVSERGSLDQ 250
    NRRLFMDDQE VAVVYFRDGY MPSQYNSQNW EARLMLERSR AAKCPDIAIQ 300
    LAGTKKVQQE LSRVGLLEAL LPGQPEAVAR LRATFAGLYS LDMGEEGDQA 350
    IAEALAAPSH FVLKPQREGG GNNLYGEEMV QALEQLKDSE ERASYILMEK 400
    IEPEPFRNCL LRPGSPAQVV QCISELGIFG VYVRQGTTLV MNKHVGHLLR 450
    TKAVEHADGG VAAGVAVLDN PYPV 474
    Length:474
    Mass (Da):52,247
    Last modified:October 1, 1996 - v1
    Checksum:iE1302C5B2C28A43A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U35456 mRNA. Translation: AAB09730.1.
    BC003784 mRNA. Translation: AAH03784.1.
    CCDSiCCDS16951.1.
    PIRiS71322.
    RefSeqiNP_032206.1. NM_008180.2.
    XP_006498844.1. XM_006498781.1.
    UniGeneiMm.252316.

    Genome annotation databases

    EnsembliENSMUST00000079691; ENSMUSP00000078630; ENSMUSG00000027610.
    GeneIDi14854.
    KEGGimmu:14854.
    UCSCiuc008nkx.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U35456 mRNA. Translation: AAB09730.1 .
    BC003784 mRNA. Translation: AAH03784.1 .
    CCDSi CCDS16951.1.
    PIRi S71322.
    RefSeqi NP_032206.1. NM_008180.2.
    XP_006498844.1. XM_006498781.1.
    UniGenei Mm.252316.

    3D structure databases

    ProteinModelPortali P51855.
    SMRi P51855. Positions 3-474.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P51855. 2 interactions.
    MINTi MINT-1854502.

    PTM databases

    PhosphoSitei P51855.

    Proteomic databases

    MaxQBi P51855.
    PaxDbi P51855.
    PRIDEi P51855.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000079691 ; ENSMUSP00000078630 ; ENSMUSG00000027610 .
    GeneIDi 14854.
    KEGGi mmu:14854.
    UCSCi uc008nkx.1. mouse.

    Organism-specific databases

    CTDi 2937.
    MGIi MGI:95852. Gss.

    Phylogenomic databases

    eggNOGi NOG329040.
    HOGENOMi HOG000172641.
    HOVERGENi HBG002458.
    InParanoidi P51855.
    KOi K01920.
    OMAi GWLLRSK.
    OrthoDBi EOG757CXD.
    PhylomeDBi P51855.
    TreeFami TF105187.

    Enzyme and pathway databases

    UniPathwayi UPA00142 ; UER00210 .
    Reactomei REACT_210980. Glutathione synthesis and recycling.
    SABIO-RK P51855.

    Miscellaneous databases

    NextBioi 287083.
    PROi P51855.
    SOURCEi Search...

    Gene expression databases

    Bgeei P51855.
    CleanExi MM_GSS.
    Genevestigatori P51855.

    Family and domain databases

    Gene3Di 1.10.1080.10. 2 hits.
    3.30.1490.50. 1 hit.
    3.30.1490.80. 2 hits.
    3.40.50.1760. 1 hit.
    InterProi IPR004887. Glutathione_synth_subst-bd_euk.
    IPR014042. Glutathione_synthase_a-hlx_euk.
    IPR014709. Glutathione_synthase_dom.
    IPR005615. Glutathione_synthase_euk.
    IPR014049. Glutathione_synthase_N_euk.
    IPR016185. PreATP-grasp_dom.
    [Graphical view ]
    PANTHERi PTHR11130. PTHR11130. 1 hit.
    Pfami PF03917. GSH_synth_ATP. 1 hit.
    PF03199. GSH_synthase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001558. GSHase. 1 hit.
    SUPFAMi SSF52440. SSF52440. 1 hit.
    TIGRFAMsi TIGR01986. glut_syn_euk. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A single mouse glutathione synthetase gene encodes six mRNAs with different 5' ends."
      Shi Z.Z., Carter B.Z., Habib G.M., He X., Sazer S., Lebovitz R.M., Lieberman M.W.
      Arch. Biochem. Biophys. 331:215-224(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
      Tissue: Kidney.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiGSHB_MOUSE
    AccessioniPrimary (citable) accession number: P51855
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3