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P51855 (GSHB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione synthetase

Short name=GSH synthetase
Short name=GSH-S
EC=6.3.2.3
Alternative name(s):
Glutathione synthase
Gene names
Name:Gss
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length474 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the eukaryotic GSH synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 474473Glutathione synthetase
PRO_0000211262

Regions

Nucleotide binding364 – 37310ATP By similarity
Nucleotide binding398 – 4014ATP By similarity
Region148 – 1514Substrate binding By similarity
Region214 – 2163Substrate binding By similarity
Region267 – 2704Substrate binding By similarity
Region461 – 4622Substrate binding By similarity

Sites

Metal binding1441Magnesium By similarity
Metal binding1461Magnesium By similarity
Metal binding3681Magnesium By similarity
Binding site1251Substrate By similarity
Binding site1441ATP By similarity
Binding site2201Substrate By similarity
Binding site3051ATP By similarity
Binding site3751ATP By similarity
Binding site4251ATP By similarity
Binding site4501Substrate By similarity
Binding site4521ATP By similarity
Binding site4581ATP; via carbonyl oxygen By similarity

Amino acid modifications

Modified residue21N-acetylalanine By similarity

Sequences

Sequence LengthMass (Da)Tools
P51855 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: E1302C5B2C28A43A

FASTA47452,247
        10         20         30         40         50         60 
MATSWGSILQ DEKQLEELAK QAIDRALAEG VLLRSAQHPS SSDVVTYAPF TLFPSPVPSA 

        70         80         90        100        110        120 
LLEQAYAVQM DFNILVDAVS QNPAFLEQTL SSTIKKDDYT ARLFDIYKQV LKEGIAQTVF 

       130        140        150        160        170        180 
LGLNRSDYMF QCGADGSKAL KQIEINTISA SFGGLASRTP AVHRHVLNVL NKTKEASKIL 

       190        200        210        220        230        240 
SNNPSKGLAL GIAKAWELYG SANAVVLLIA QEKERNIFDQ RAVENELLDR KIHVIRGRFE 

       250        260        270        280        290        300 
DVSERGSLDQ NRRLFMDDQE VAVVYFRDGY MPSQYNSQNW EARLMLERSR AAKCPDIAIQ 

       310        320        330        340        350        360 
LAGTKKVQQE LSRVGLLEAL LPGQPEAVAR LRATFAGLYS LDMGEEGDQA IAEALAAPSH 

       370        380        390        400        410        420 
FVLKPQREGG GNNLYGEEMV QALEQLKDSE ERASYILMEK IEPEPFRNCL LRPGSPAQVV 

       430        440        450        460        470 
QCISELGIFG VYVRQGTTLV MNKHVGHLLR TKAVEHADGG VAAGVAVLDN PYPV 

« Hide

References

« Hide 'large scale' references
[1]"A single mouse glutathione synthetase gene encodes six mRNAs with different 5' ends."
Shi Z.Z., Carter B.Z., Habib G.M., He X., Sazer S., Lebovitz R.M., Lieberman M.W.
Arch. Biochem. Biophys. 331:215-224(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
Tissue: Kidney.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U35456 mRNA. Translation: AAB09730.1.
BC003784 mRNA. Translation: AAH03784.1.
PIRS71322.
RefSeqNP_032206.1. NM_008180.1.
XP_006498844.1. XM_006498781.1.
UniGeneMm.252316.

3D structure databases

ProteinModelPortalP51855.
SMRP51855. Positions 3-474.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP51855. 2 interactions.
MINTMINT-1854502.

PTM databases

PhosphoSiteP51855.

Proteomic databases

PaxDbP51855.
PRIDEP51855.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000079691; ENSMUSP00000078630; ENSMUSG00000027610.
GeneID14854.
KEGGmmu:14854.
UCSCuc008nkx.1. mouse.

Organism-specific databases

CTD2937.
MGIMGI:95852. Gss.

Phylogenomic databases

eggNOGNOG329040.
HOGENOMHOG000172641.
HOVERGENHBG002458.
InParanoidP51855.
KOK01920.
OMAFENCLLR.
OrthoDBEOG757CXD.
PhylomeDBP51855.
TreeFamTF105187.

Enzyme and pathway databases

SABIO-RKP51855.
UniPathwayUPA00142; UER00210.

Gene expression databases

BgeeP51855.
CleanExMM_GSS.
GenevestigatorP51855.

Family and domain databases

Gene3D1.10.1080.10. 2 hits.
3.30.1490.50. 1 hit.
3.30.1490.80. 2 hits.
3.40.50.1760. 1 hit.
InterProIPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR11130. PTHR11130. 1 hit.
PfamPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFPIRSF001558. GSHase. 1 hit.
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01986. glut_syn_euk. 1 hit.
ProtoNetSearch...

Other

NextBio287083.
PROP51855.
SOURCESearch...

Entry information

Entry nameGSHB_MOUSE
AccessionPrimary (citable) accession number: P51855
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot