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Protein

Glutathione synthetase

Gene

Gss

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Pathway:iglutathione biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate--cysteine ligase catalytic subunit (Gclc), Glutamate--cysteine ligase regulatory subunit (Gclm)
  2. Glutathione synthetase (Gss), Glutathione synthetase (Gss), Glutathione synthetase (Gss)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei125 – 1251SubstrateBy similarity
Metal bindingi144 – 1441MagnesiumBy similarity
Binding sitei144 – 1441ATPBy similarity
Metal bindingi146 – 1461MagnesiumBy similarity
Binding sitei220 – 2201SubstrateBy similarity
Binding sitei305 – 3051ATPBy similarity
Metal bindingi368 – 3681MagnesiumBy similarity
Binding sitei375 – 3751ATPBy similarity
Binding sitei425 – 4251ATPBy similarity
Binding sitei450 – 4501SubstrateBy similarity
Binding sitei452 – 4521ATPBy similarity
Binding sitei458 – 4581ATP; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi364 – 37310ATPBy similarity
Nucleotide bindingi398 – 4014ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_319124. Glutathione synthesis and recycling.
SABIO-RKP51855.
UniPathwayiUPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetase (EC:6.3.2.3)
Short name:
GSH synthetase
Short name:
GSH-S
Alternative name(s):
Glutathione synthase
Gene namesi
Name:Gss
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:95852. Gss.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 474473Glutathione synthetasePRO_0000211262Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei415 – 4151PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP51855.
PaxDbiP51855.
PRIDEiP51855.

PTM databases

PhosphoSiteiP51855.

Expressioni

Gene expression databases

BgeeiP51855.
CleanExiMM_GSS.
ExpressionAtlasiP51855. baseline and differential.
GenevisibleiP51855. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiP51855. 2 interactions.
MINTiMINT-1854502.
STRINGi10090.ENSMUSP00000078630.

Structurei

3D structure databases

ProteinModelPortaliP51855.
SMRiP51855. Positions 3-474.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni148 – 1514Substrate bindingBy similarity
Regioni214 – 2163Substrate bindingBy similarity
Regioni267 – 2704Substrate bindingBy similarity
Regioni461 – 4622Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the eukaryotic GSH synthase family.Curated

Phylogenomic databases

eggNOGiNOG329040.
GeneTreeiENSGT00390000013764.
HOGENOMiHOG000172641.
HOVERGENiHBG002458.
InParanoidiP51855.
OMAiFENCLLR.
OrthoDBiEOG757CXD.
PhylomeDBiP51855.
TreeFamiTF105187.

Family and domain databases

Gene3Di1.10.1080.10. 2 hits.
3.30.1490.50. 1 hit.
3.30.1490.80. 2 hits.
3.40.50.1760. 1 hit.
InterProiIPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR11130. PTHR11130. 1 hit.
PfamiPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001558. GSHase. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P51855-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATSWGSILQ DEKQLEELAK QAIDRALAEG VLLRSAQHPS SSDVVTYAPF
60 70 80 90 100
TLFPSPVPSA LLEQAYAVQM DFNILVDAVS QNPAFLEQTL SSTIKKDDYT
110 120 130 140 150
ARLFDIYKQV LKEGIAQTVF LGLNRSDYMF QCGADGSKAL KQIEINTISA
160 170 180 190 200
SFGGLASRTP AVHRHVLNVL NKTKEASKIL SNNPSKGLAL GIAKAWELYG
210 220 230 240 250
SANAVVLLIA QEKERNIFDQ RAVENELLDR KIHVIRGRFE DVSERGSLDQ
260 270 280 290 300
NRRLFMDDQE VAVVYFRDGY MPSQYNSQNW EARLMLERSR AAKCPDIAIQ
310 320 330 340 350
LAGTKKVQQE LSRVGLLEAL LPGQPEAVAR LRATFAGLYS LDMGEEGDQA
360 370 380 390 400
IAEALAAPSH FVLKPQREGG GNNLYGEEMV QALEQLKDSE ERASYILMEK
410 420 430 440 450
IEPEPFRNCL LRPGSPAQVV QCISELGIFG VYVRQGTTLV MNKHVGHLLR
460 470
TKAVEHADGG VAAGVAVLDN PYPV
Length:474
Mass (Da):52,247
Last modified:October 1, 1996 - v1
Checksum:iE1302C5B2C28A43A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35456 mRNA. Translation: AAB09730.1.
BC003784 mRNA. Translation: AAH03784.1.
CCDSiCCDS16951.1.
PIRiS71322.
RefSeqiNP_032206.1. NM_008180.2.
XP_006498844.1. XM_006498781.2.
UniGeneiMm.252316.

Genome annotation databases

EnsembliENSMUST00000079691; ENSMUSP00000078630; ENSMUSG00000027610.
GeneIDi14854.
UCSCiuc008nkx.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35456 mRNA. Translation: AAB09730.1.
BC003784 mRNA. Translation: AAH03784.1.
CCDSiCCDS16951.1.
PIRiS71322.
RefSeqiNP_032206.1. NM_008180.2.
XP_006498844.1. XM_006498781.2.
UniGeneiMm.252316.

3D structure databases

ProteinModelPortaliP51855.
SMRiP51855. Positions 3-474.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP51855. 2 interactions.
MINTiMINT-1854502.
STRINGi10090.ENSMUSP00000078630.

PTM databases

PhosphoSiteiP51855.

Proteomic databases

MaxQBiP51855.
PaxDbiP51855.
PRIDEiP51855.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000079691; ENSMUSP00000078630; ENSMUSG00000027610.
GeneIDi14854.
UCSCiuc008nkx.2. mouse.

Organism-specific databases

CTDi2937.
MGIiMGI:95852. Gss.

Phylogenomic databases

eggNOGiNOG329040.
GeneTreeiENSGT00390000013764.
HOGENOMiHOG000172641.
HOVERGENiHBG002458.
InParanoidiP51855.
OMAiFENCLLR.
OrthoDBiEOG757CXD.
PhylomeDBiP51855.
TreeFamiTF105187.

Enzyme and pathway databases

UniPathwayiUPA00142; UER00210.
ReactomeiREACT_319124. Glutathione synthesis and recycling.
SABIO-RKP51855.

Miscellaneous databases

NextBioi287083.
PROiP51855.
SOURCEiSearch...

Gene expression databases

BgeeiP51855.
CleanExiMM_GSS.
ExpressionAtlasiP51855. baseline and differential.
GenevisibleiP51855. MM.

Family and domain databases

Gene3Di1.10.1080.10. 2 hits.
3.30.1490.50. 1 hit.
3.30.1490.80. 2 hits.
3.40.50.1760. 1 hit.
InterProiIPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR11130. PTHR11130. 1 hit.
PfamiPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001558. GSHase. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A single mouse glutathione synthetase gene encodes six mRNAs with different 5' ends."
    Shi Z.Z., Carter B.Z., Habib G.M., He X., Sazer S., Lebovitz R.M., Lieberman M.W.
    Arch. Biochem. Biophys. 331:215-224(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Kidney.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiGSHB_MOUSE
AccessioniPrimary (citable) accession number: P51855
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 22, 2015
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.