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Protein

Nuclear receptor subfamily 0 group B member 1

Gene

NR0B1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency (By similarity).By similarity

GO - Molecular functioni

  • AF-2 domain binding Source: UniProtKB
  • DNA binding Source: HGNC
  • DNA hairpin binding Source: HGNC
  • protein domain specific binding Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB
  • RNA binding Source: HGNC
  • RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding Source: UniProtKB
  • sequence-specific DNA binding Source: HGNC
  • steroid hormone receptor activity Source: InterPro
  • steroid hormone receptor binding Source: UniProtKB
  • transcription corepressor activity Source: BHF-UCL
  • transcription factor binding Source: BHF-UCL

GO - Biological processi

  • adrenal gland development Source: HGNC
  • gonad development Source: HGNC
  • hypothalamus development Source: UniProtKB
  • Leydig cell differentiation Source: Ensembl
  • male gonad development Source: UniProtKB
  • male sex determination Source: Ensembl
  • negative regulation of cell differentiation Source: Ensembl
  • negative regulation of intracellular steroid hormone receptor signaling pathway Source: UniProtKB
  • negative regulation of sequence-specific DNA binding transcription factor activity Source: BHF-UCL
  • negative regulation of transcription, DNA-templated Source: HGNC
  • negative regulation of transcription from RNA polymerase II promoter Source: GO_Central
  • pituitary gland development Source: UniProtKB
  • protein localization Source: UniProtKB
  • response to immobilization stress Source: Ensembl
  • Sertoli cell differentiation Source: Ensembl
  • spermatogenesis Source: Ensembl
  • steroid biosynthetic process Source: HGNC
  • transcription initiation from RNA polymerase II promoter Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Receptor, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-383280. Nuclear Receptor transcription pathway.
SignaLinkiP51843.
SIGNORiP51843.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear receptor subfamily 0 group B member 1
Alternative name(s):
DSS-AHC critical region on the X chromosome protein 1
Nuclear receptor DAX-1
Gene namesi
Name:NR0B1
Synonyms:AHC, DAX1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

HGNCiHGNC:7960. NR0B1.

Subcellular locationi

  • Nucleus 1 Publication
  • Cytoplasm 1 Publication

  • Note: Shuttles between the cytoplasm and nucleus. Homodimers exits in the cytoplasm and in the nucleus.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • membrane Source: BHF-UCL
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
  • polysomal ribosome Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Involvement in diseasei

X-linked adrenal hypoplasia congenital (XL-AHC)14 Publications
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionDevelopmental disorder of the adrenal gland that results in profound hormonal deficiencies and is lethal if untreated. It is characterized by the absence of the permanent zone of the adrenal cortex and by a structural disorganization of the glands. Hypogonadotropic hypogonadism (HHG) is frequently associated with this disorder. HHG is a condition resulting from or characterized by abnormally decreased gonadal function, with retardation of growth and sexual development.
See also OMIM:300200
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti267 – 2671R → P in XL-AHC; impairs transcriptional silencing of the StAR promoter. 3 Publications
Corresponds to variant rs104894888 [ dbSNP | Ensembl ].
VAR_004738
Natural varianti269 – 2691Missing in XL-AHC; impairs transcriptional silencing of the StAR promoter. 3 Publications
VAR_004739
Natural varianti278 – 2781L → P in XL-AHC. 1 Publication
VAR_031079
Natural varianti287 – 2871V → G in XL-AHC; the patient presents an inappropriate tall stature and renal ectopy. 1 Publication
VAR_004740
Natural varianti291 – 2911W → C in XL-AHC. 1 Publication
Corresponds to variant rs28935482 [ dbSNP | Ensembl ].
VAR_031080
Natural varianti295 – 2951L → P in XL-AHC. 1 Publication
VAR_018303
Natural varianti297 – 2971L → P in XL-AHC; results in a severe loss of repressor activity. 1 Publication
Corresponds to variant rs104894907 [ dbSNP | Ensembl ].
VAR_031081
Natural varianti300 – 3001A → P in XL-AHC. 1 Publication
VAR_018304
Natural varianti300 – 3001A → V in XL-AHC. 1 Publication
VAR_004741
Natural varianti377 – 3771E → K in XL-AHC. 2 Publications
VAR_004742
Natural varianti380 – 3801Y → D in XL-AHC. 1 Publication
Corresponds to variant rs104894900 [ dbSNP | Ensembl ].
VAR_018300
Natural varianti381 – 3811L → H in XL-AHC. 1 Publication
Corresponds to variant rs104894899 [ dbSNP | Ensembl ].
VAR_018301
Natural varianti382 – 3821K → N in XL-AHC. 1 Publication
Corresponds to variant rs28935180 [ dbSNP | Ensembl ].
VAR_004743
Natural varianti385 – 3851V → G in XL-AHC. 1 Publication
VAR_004744
Natural varianti425 – 4251R → G in XL-AHC. 1 Publication
VAR_004745
Natural varianti425 – 4251R → T in XL-AHC. 1 Publication
VAR_018305
Natural varianti439 – 4391I → S in XL-AHC; mild phenotype. 1 Publication
Corresponds to variant rs104894897 [ dbSNP | Ensembl ].
VAR_018302
Natural varianti440 – 4401N → I in XL-AHC; impairs RNA-binding activity. 2 Publications
Corresponds to variant rs28935481 [ dbSNP | Ensembl ].
VAR_004746
Natural varianti466 – 4661L → R in XL-AHC. 1 Publication
VAR_018306
46,XY sex reversal 2 (SRXY2)1 Publication
The disease is caused by mutations affecting the gene represented in this entry. XY individuals with a duplication of part of the short arm of the X chromosome and an intact SRY gene develop as females. The single X chromosome in these individuals does not undergo X-chromosome inactivation; therefore, these individuals presumably carry 2 active copies of genes, including the NR0B1 gene, in the duplicated region. Individuals with deletion of this region develop as males. Genes within the dosage-sensitive sex reversal region are, therefore, not essential for testis development, but, when present in a double dose, interfere with testis formation.
Disease descriptionA condition characterized by male-to-female sex reversal in the presence of a normal 46,XY karyotype.
See also OMIM:300018

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi16 – 172ML → AA: Strongly reduces homodimerization and interaction with NR0B2. 1 Publication
Mutagenesisi83 – 842ML → AA: Strongly reduces homodimerization and interaction with NR0B2. 1 Publication
Mutagenesisi149 – 1502LL → AA: Strongly reduces homodimerization and interaction with NR0B2. 1 Publication
Mutagenesisi461 – 4622MM → AA: Strongly reduces homodimerization and interaction with NR0B2. 1 Publication

Keywords - Diseasei

Disease mutation

Organism-specific databases

MalaCardsiNR0B1.
MIMi300018. phenotype.
300200. phenotype.
Orphaneti242. 46,XY complete gonadal dysgenesis.
251510. 46,XY partial gonadal dysgenesis.
95702. Cytomegalic congenital adrenal hypoplasia.
PharmGKBiPA31746.

Chemistry

ChEMBLiCHEMBL1795094.
DrugBankiDB01234. Dexamethasone.
DB00755. Tretinoin.

Polymorphism and mutation databases

BioMutaiNR0B1.
DMDMi20532385.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 470470Nuclear receptor subfamily 0 group B member 1PRO_0000053748Add
BLAST

Proteomic databases

EPDiP51843.
MaxQBiP51843.
PaxDbiP51843.
PeptideAtlasiP51843.
PRIDEiP51843.

PTM databases

iPTMnetiP51843.
PhosphoSiteiP51843.

Expressioni

Gene expression databases

BgeeiENSG00000169297.
CleanExiHS_NR0B1.
ExpressionAtlasiP51843. baseline and differential.
GenevisibleiP51843. HS.

Organism-specific databases

HPAiHPA070839.

Interactioni

Subunit structurei

Homodimer. Interacts with NR5A1, NR5A2, NR0B2 and with COPS2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ESRRGP625083EBI-946109,EBI-2834260
NR5A1Q132853EBI-946109,EBI-874629
RORAP353982EBI-946109,EBI-748689

GO - Molecular functioni

  • AF-2 domain binding Source: UniProtKB
  • protein domain specific binding Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB
  • steroid hormone receptor binding Source: UniProtKB
  • transcription factor binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi106695. 14 interactions.
IntActiP51843. 3 interactions.
MINTiMINT-2855705.
STRINGi9606.ENSP00000368253.

Chemistry

BindingDBiP51843.

Structurei

Secondary structure

1
470
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi145 – 1517Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4RWVX-ray1.86B140-154[»]
ProteinModelPortaliP51843.
SMRiP51843. Positions 207-470.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati1 – 67671Add
BLAST
Repeati68 – 133662Add
BLAST
Repeati134 – 200673Add
BLAST
Repeati201 – 253534; truncatedAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 2532534 X 67 AA tandem repeatsAdd
BLAST
Regioni254 – 470217Ligand-bindingBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi13 – 175LXXLL motif 1
Motifi80 – 845LXXLL motif 2
Motifi146 – 1505LXXLL motif 3
Motifi461 – 4666AF-2 motif

Domaini

Homodimerization involved an interaction between amino and carboxy termini involving LXXLL motifs and steroid binding domain (AF-2 motif). Heterodimerizes with NR5A1 and NROB2 through its N-terminal LXXLL motifs.

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3575. Eukaryota.
ENOG410XRZC. LUCA.
GeneTreeiENSGT00390000015719.
HOGENOMiHOG000231151.
HOVERGENiHBG005453.
InParanoidiP51843.
KOiK08562.
OMAiGPCWGCS.
OrthoDBiEOG091G0FGK.
PhylomeDBiP51843.
TreeFamiTF332386.

Family and domain databases

Gene3Di1.10.565.10. 1 hit.
InterProiIPR033544. NR0B1.
IPR000536. Nucl_hrmn_rcpt_lig-bd.
IPR001723. Nuclear_hrmn_rcpt.
IPR025900. Nuclear_receptor_repeat.
[Graphical view]
PANTHERiPTHR24081:SF1. PTHR24081:SF1. 3 hits.
PfamiPF00104. Hormone_recep. 1 hit.
PF14046. NR_Repeat. 4 hits.
[Graphical view]
PRINTSiPR00398. STRDHORMONER.
SMARTiSM00430. HOLI. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P51843-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGENHQWQG SILYNMLMSA KQTRAAPEAP ETRLVDQCWG CSCGDEPGVG
60 70 80 90 100
REGLLGGRNV ALLYRCCFCG KDHPRQGSIL YSMLTSAKQT YAAPKAPEAT
110 120 130 140 150
LGPCWGCSCG SDPGVGRAGL PGGRPVALLY RCCFCGEDHP RQGSILYSLL
160 170 180 190 200
TSSKQTHVAP AAPEARPGGA WWDRSYFAQR PGGKEALPGG RATALLYRCC
210 220 230 240 250
FCGEDHPQQG STLYCVPTST NQAQAAPEER PRAPWWDTSS GALRPVALKS
260 270 280 290 300
PQVVCEAASA GLLKTLRFVK YLPCFQVLPL DQQLVLVRNC WASLLMLELA
310 320 330 340 350
QDRLQFETVE VSEPSMLQKI LTTRRRETGG NEPLPVPTLQ HHLAPPAEAR
360 370 380 390 400
KVPSASQVQA IKCFLSKCWS LNISTKEYAY LKGTVLFNPD VPGLQCVKYI
410 420 430 440 450
QGLQWGTQQI LSEHTRMTHQ GPHDRFIELN STLFLLRFIN ANVIAELFFR
460 470
PIIGTVSMDD MMLEMLCTKI
Length:470
Mass (Da):51,718
Last modified:May 10, 2002 - v2
Checksum:i214E237097DF9786
GO
Isoform 2 (identifier: P51843-2) [UniParc]FASTAAdd to basket
Also known as: NR0B1A

The sequence of this isoform differs from the canonical sequence as follows:
     390-400: DVPGLQCVKYI → GKGKENDCNHH
     401-470: Missing.

Note: More abundant than isoform 1 in all tissues tested except testis where they are nearly equal.
Show »
Length:400
Mass (Da):43,590
Checksum:i368BB8D21A210658
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41E → Q in AAC13875 (PubMed:8675564).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti267 – 2671R → P in XL-AHC; impairs transcriptional silencing of the StAR promoter. 3 Publications
Corresponds to variant rs104894888 [ dbSNP | Ensembl ].
VAR_004738
Natural varianti269 – 2691Missing in XL-AHC; impairs transcriptional silencing of the StAR promoter. 3 Publications
VAR_004739
Natural varianti278 – 2781L → P in XL-AHC. 1 Publication
VAR_031079
Natural varianti287 – 2871V → G in XL-AHC; the patient presents an inappropriate tall stature and renal ectopy. 1 Publication
VAR_004740
Natural varianti291 – 2911W → C in XL-AHC. 1 Publication
Corresponds to variant rs28935482 [ dbSNP | Ensembl ].
VAR_031080
Natural varianti295 – 2951L → P in XL-AHC. 1 Publication
VAR_018303
Natural varianti297 – 2971L → P in XL-AHC; results in a severe loss of repressor activity. 1 Publication
Corresponds to variant rs104894907 [ dbSNP | Ensembl ].
VAR_031081
Natural varianti300 – 3001A → P in XL-AHC. 1 Publication
VAR_018304
Natural varianti300 – 3001A → V in XL-AHC. 1 Publication
VAR_004741
Natural varianti377 – 3771E → K in XL-AHC. 2 Publications
VAR_004742
Natural varianti380 – 3801Y → D in XL-AHC. 1 Publication
Corresponds to variant rs104894900 [ dbSNP | Ensembl ].
VAR_018300
Natural varianti381 – 3811L → H in XL-AHC. 1 Publication
Corresponds to variant rs104894899 [ dbSNP | Ensembl ].
VAR_018301
Natural varianti382 – 3821K → N in XL-AHC. 1 Publication
Corresponds to variant rs28935180 [ dbSNP | Ensembl ].
VAR_004743
Natural varianti385 – 3851V → G in XL-AHC. 1 Publication
VAR_004744
Natural varianti425 – 4251R → G in XL-AHC. 1 Publication
VAR_004745
Natural varianti425 – 4251R → T in XL-AHC. 1 Publication
VAR_018305
Natural varianti439 – 4391I → S in XL-AHC; mild phenotype. 1 Publication
Corresponds to variant rs104894897 [ dbSNP | Ensembl ].
VAR_018302
Natural varianti440 – 4401N → I in XL-AHC; impairs RNA-binding activity. 2 Publications
Corresponds to variant rs28935481 [ dbSNP | Ensembl ].
VAR_004746
Natural varianti466 – 4661L → R in XL-AHC. 1 Publication
VAR_018306

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei390 – 40011DVPGLQCVKYI → GKGKENDCNHH in isoform 2. CuratedVSP_023557Add
BLAST
Alternative sequencei401 – 47070Missing in isoform 2. CuratedVSP_023558Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S74720 mRNA. Translation: AAB32751.1.
U31929 Genomic DNA. Translation: AAC13875.1.
BC011564 mRNA. Translation: AAH11564.1.
CCDSiCCDS14223.1. [P51843-1]
PIRiS50854.
RefSeqiNP_000466.2. NM_000475.4. [P51843-1]
UniGeneiHs.268490.

Genome annotation databases

EnsembliENST00000378970; ENSP00000368253; ENSG00000169297. [P51843-1]
GeneIDi190.
KEGGihsa:190.
UCSCiuc004dcf.5. human. [P51843-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S74720 mRNA. Translation: AAB32751.1.
U31929 Genomic DNA. Translation: AAC13875.1.
BC011564 mRNA. Translation: AAH11564.1.
CCDSiCCDS14223.1. [P51843-1]
PIRiS50854.
RefSeqiNP_000466.2. NM_000475.4. [P51843-1]
UniGeneiHs.268490.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4RWVX-ray1.86B140-154[»]
ProteinModelPortaliP51843.
SMRiP51843. Positions 207-470.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106695. 14 interactions.
IntActiP51843. 3 interactions.
MINTiMINT-2855705.
STRINGi9606.ENSP00000368253.

Chemistry

BindingDBiP51843.
ChEMBLiCHEMBL1795094.
DrugBankiDB01234. Dexamethasone.
DB00755. Tretinoin.

PTM databases

iPTMnetiP51843.
PhosphoSiteiP51843.

Polymorphism and mutation databases

BioMutaiNR0B1.
DMDMi20532385.

Proteomic databases

EPDiP51843.
MaxQBiP51843.
PaxDbiP51843.
PeptideAtlasiP51843.
PRIDEiP51843.

Protocols and materials databases

DNASUi190.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000378970; ENSP00000368253; ENSG00000169297. [P51843-1]
GeneIDi190.
KEGGihsa:190.
UCSCiuc004dcf.5. human. [P51843-1]

Organism-specific databases

CTDi190.
GeneCardsiNR0B1.
GeneReviewsiNR0B1.
HGNCiHGNC:7960. NR0B1.
HPAiHPA070839.
MalaCardsiNR0B1.
MIMi300018. phenotype.
300200. phenotype.
300473. gene.
neXtProtiNX_P51843.
Orphaneti242. 46,XY complete gonadal dysgenesis.
251510. 46,XY partial gonadal dysgenesis.
95702. Cytomegalic congenital adrenal hypoplasia.
PharmGKBiPA31746.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3575. Eukaryota.
ENOG410XRZC. LUCA.
GeneTreeiENSGT00390000015719.
HOGENOMiHOG000231151.
HOVERGENiHBG005453.
InParanoidiP51843.
KOiK08562.
OMAiGPCWGCS.
OrthoDBiEOG091G0FGK.
PhylomeDBiP51843.
TreeFamiTF332386.

Enzyme and pathway databases

ReactomeiR-HSA-383280. Nuclear Receptor transcription pathway.
SignaLinkiP51843.
SIGNORiP51843.

Miscellaneous databases

GeneWikiiDAX1.
GenomeRNAii190.
PROiP51843.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000169297.
CleanExiHS_NR0B1.
ExpressionAtlasiP51843. baseline and differential.
GenevisibleiP51843. HS.

Family and domain databases

Gene3Di1.10.565.10. 1 hit.
InterProiIPR033544. NR0B1.
IPR000536. Nucl_hrmn_rcpt_lig-bd.
IPR001723. Nuclear_hrmn_rcpt.
IPR025900. Nuclear_receptor_repeat.
[Graphical view]
PANTHERiPTHR24081:SF1. PTHR24081:SF1. 3 hits.
PfamiPF00104. Hormone_recep. 1 hit.
PF14046. NR_Repeat. 4 hits.
[Graphical view]
PRINTSiPR00398. STRDHORMONER.
SMARTiSM00430. HOLI. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNR0B1_HUMAN
AccessioniPrimary (citable) accession number: P51843
Secondary accession number(s): Q96F69
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 10, 2002
Last modified: September 7, 2016
This is version 169 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.