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P51842

- GUC2F_RAT

UniProt

P51842 - GUC2F_RAT

Protein

Retinal guanylyl cyclase 2

Gene

Gucy2f

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Probably plays a specific functional role in the rods and/or cones of photoreceptors. It may be the enzyme involved in the resynthesis of cGMP required for recovery of the dark state after phototransduction.

    Catalytic activityi

    GTP = 3',5'-cyclic GMP + diphosphate.

    GO - Molecular functioni

    1. ATP binding Source: InterPro
    2. GTP binding Source: UniProtKB-KW
    3. guanylate cyclase activity Source: RGD
    4. protein binding Source: RGD
    5. protein heterodimerization activity Source: RGD
    6. protein homodimerization activity Source: RGD
    7. protein kinase activity Source: InterPro

    GO - Biological processi

    1. intracellular signal transduction Source: InterPro
    2. visual perception Source: UniProtKB-KW

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    cGMP biosynthesis, Sensory transduction, Vision

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_227662. Inactivation, recovery and regulation of the phototransduction cascade.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Retinal guanylyl cyclase 2 (EC:4.6.1.2)
    Short name:
    RETGC-2
    Alternative name(s):
    Guanylate cyclase 2F, retinal
    Guanylate cyclase F
    Short name:
    GC-F
    Rod outer segment membrane guanylate cyclase 2
    Short name:
    ROS-GC2
    Gene namesi
    Name:Gucy2f
    Synonyms:Guc2f
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome X

    Organism-specific databases

    RGDi620439. Gucy2f.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. membrane Source: RGD

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 5050Sequence AnalysisAdd
    BLAST
    Chaini51 – 11081058Retinal guanylyl cyclase 2PRO_0000012387Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi104 ↔ 132By similarity
    Disulfide bondi452 – 452InterchainBy similarity
    Disulfide bondi460 – 460InterchainBy similarity

    Post-translational modificationi

    There are 9 conserved cysteine residues in sensory guanylate cyclases, 6 in the extracellular domain, which may be involved in intra- or interchain disulfide bonds.

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiP51842.
    PRIDEiP51842.

    PTM databases

    PhosphoSiteiP51842.

    Expressioni

    Tissue specificityi

    Expressed only in the eye.

    Gene expression databases

    GenevestigatoriP51842.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000049500.

    Structurei

    3D structure databases

    ProteinModelPortaliP51842.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini51 – 465415ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini491 – 1108618CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei466 – 49025HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini532 – 812281Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini884 – 1014131Guanylate cyclasePROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The protein kinase domain is predicted to be catalytically inactive.

    Sequence similaritiesi

    Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation
    Contains 1 guanylate cyclase domain.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00730000110520.
    HOGENOMiHOG000293307.
    HOVERGENiHBG098487.
    InParanoidiP51842.
    KOiK12321.
    OMAiNSIYFIA.
    OrthoDBiEOG7QVM1V.
    PhylomeDBiP51842.
    TreeFamiTF106338.

    Family and domain databases

    Gene3Di3.30.70.1230. 1 hit.
    InterProiIPR001054. A/G_cyclase.
    IPR018297. A/G_cyclase_CS.
    IPR001828. ANF_lig-bd_rcpt.
    IPR011645. Haem_no_assoc-bd.
    IPR011009. Kinase-like_dom.
    IPR028082. Peripla_BP_I.
    IPR000719. Prot_kinase_dom.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    [Graphical view]
    PfamiPF01094. ANF_receptor. 1 hit.
    PF00211. Guanylate_cyc. 1 hit.
    PF07701. HNOBA. 1 hit.
    PF07714. Pkinase_Tyr. 1 hit.
    [Graphical view]
    SMARTiSM00044. CYCc. 1 hit.
    [Graphical view]
    SUPFAMiSSF53822. SSF53822. 1 hit.
    SSF55073. SSF55073. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS00452. GUANYLATE_CYCLASE_1. 1 hit.
    PS50125. GUANYLATE_CYCLASE_2. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P51842-1 [UniParc]FASTAAdd to Basket

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    MFLGPWPFSR LLSWFAISSR LSGQHGLTSS KFLRYLCLLA LLPLIWWGQA     50
    LPYKIGVIGP WTCDPFFSKA LPEVAAALAI ERISRDMSFD RSYSFEYVIL 100
    NEDCQTSKAL TSFISHQQMA SGFVGPANPG YCEAASLLGN SWDKGIFSWA 150
    CVNHELDNKH SYPTFSRTLP SPIRVLVTVM KYFQWAHAGV ISSDEDIWVH 200
    TANQVSSALR SHGLPVGVVL TSGQDSRSIQ KALQQIRQAD RIRIIIMCMH 250
    SALIGGETQT HFLELAHDLK MTDGTYVFVP YDVLLYSLPY KHSPYQVLRN 300
    NQKLREAYDA VLTITVESHE KTFYEAFTEA AAGGEIPEKL DSHQVSPLFG 350
    TIYNSIYFIA QAMSNALKEN GQASAASLTR HSRNMQFYGF NQLIRTDSNG 400
    NGISEYVILD TNGKEWELRG TYTVDMETEL LRFRGTPIHF PGGRPTSADA 450
    KCWFAQGKIC QGGIDPALAM MVCFALLLAL LSINGFAYFI RRRINKIQLI 500
    KGPNRILLTL EDVTFINPHF GSKRGSRASV SFQIISEVQS GRSPRLSFSS 550
    GSLTPATYEN SNIAIYQGDW VWLKKFPPGD FGDIKSIKSS ASDVFEMMKD 600
    LRHENVNPLL GFFYDSGMFA IVSEFCSRRS LEDILTQDDV KLDWMFKSSL 650
    LLDLIKGMKY LHHREFIHGR LKSRNCVVDG RFVLKVTDYG FNNILEMLRL 700
    SEEEPSEEEL LWTAPELLRA PGGIRLGSFA GDVYSFAIIM QEVMVRGAPF 750
    CMMDLSAKEV IDRLKMPPPV YRPVVSPEFA PPECLQLMKQ CWAEAAEQRP 800
    TFDEIFNQFK TFNKGKKTNI IDSMLRMLEQ YSSNLEDLIR ERTEELEIEK 850
    QKTEKLLTQM LPPSVAESLK KGCTVEPEGF DLVTLYFSDI VGFTTISAMS 900
    EPIEVVDLLN DLYTLFDAII GSHDVYKVET IGDAYMVASG LPKRNGSRHA 950
    AEIANMSLDI LSSVGTFKMR HMPEVPVRIR IGLHTGPVVA GVVGLTMPRY 1000
    CLFGDTVNTA SRMESTGLPY RIHVSLSTVT ILRTLSEGYE VELRGRTELK 1050
    GKGTEETFWL VGKKGFTKPL PVPPPVGKDG QVGHGLQPAE IAAFQRRKAE 1100
    RQLVRNKP 1108
    Length:1,108
    Mass (Da):124,411
    Last modified:October 1, 1996 - v1
    Checksum:i2A9147AF2202E4E3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L36030 mRNA. Translation: AAA65511.1.
    PIRiB55915.
    RefSeqiNP_446283.1. NM_053831.1.
    UniGeneiRn.10061.

    Genome annotation databases

    EnsembliENSRNOT00000046804; ENSRNOP00000049500; ENSRNOG00000019086.
    GeneIDi116556.
    KEGGirno:116556.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L36030 mRNA. Translation: AAA65511.1 .
    PIRi B55915.
    RefSeqi NP_446283.1. NM_053831.1.
    UniGenei Rn.10061.

    3D structure databases

    ProteinModelPortali P51842.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000049500.

    PTM databases

    PhosphoSitei P51842.

    Proteomic databases

    PaxDbi P51842.
    PRIDEi P51842.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000046804 ; ENSRNOP00000049500 ; ENSRNOG00000019086 .
    GeneIDi 116556.
    KEGGi rno:116556.

    Organism-specific databases

    CTDi 2986.
    RGDi 620439. Gucy2f.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00730000110520.
    HOGENOMi HOG000293307.
    HOVERGENi HBG098487.
    InParanoidi P51842.
    KOi K12321.
    OMAi NSIYFIA.
    OrthoDBi EOG7QVM1V.
    PhylomeDBi P51842.
    TreeFami TF106338.

    Enzyme and pathway databases

    Reactomei REACT_227662. Inactivation, recovery and regulation of the phototransduction cascade.

    Miscellaneous databases

    NextBioi 619209.
    PROi P51842.

    Gene expression databases

    Genevestigatori P51842.

    Family and domain databases

    Gene3Di 3.30.70.1230. 1 hit.
    InterProi IPR001054. A/G_cyclase.
    IPR018297. A/G_cyclase_CS.
    IPR001828. ANF_lig-bd_rcpt.
    IPR011645. Haem_no_assoc-bd.
    IPR011009. Kinase-like_dom.
    IPR028082. Peripla_BP_I.
    IPR000719. Prot_kinase_dom.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    [Graphical view ]
    Pfami PF01094. ANF_receptor. 1 hit.
    PF00211. Guanylate_cyc. 1 hit.
    PF07701. HNOBA. 1 hit.
    PF07714. Pkinase_Tyr. 1 hit.
    [Graphical view ]
    SMARTi SM00044. CYCc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53822. SSF53822. 1 hit.
    SSF55073. SSF55073. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS00452. GUANYLATE_CYCLASE_1. 1 hit.
    PS50125. GUANYLATE_CYCLASE_2. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Eye.

    Entry informationi

    Entry nameiGUC2F_RAT
    AccessioniPrimary (citable) accession number: P51842
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 127 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3