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Protein

Retinal guanylyl cyclase 2

Gene

GUCY2F

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Probably plays a specific functional role in the rods and/or cones of photoreceptors. It may be the enzyme involved in the resynthesis of cGMP required for recovery of the dark state after phototransduction.

Catalytic activityi

GTP = 3',5'-cyclic GMP + diphosphate.

Enzyme regulationi

Activated by GCAP-1; inhibited by calcium.

GO - Molecular functioni

  • ATP binding Source: InterPro
  • GTP binding Source: UniProtKB-KW
  • guanylate cyclase activity Source: Reactome
  • protein kinase activity Source: InterPro
  • receptor activity Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

cGMP biosynthesis, Sensory transduction, Vision

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS02314-MONOMER.
BRENDAi4.6.1.2. 2681.
ReactomeiR-HSA-2514859. Inactivation, recovery and regulation of the phototransduction cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
Retinal guanylyl cyclase 2 (EC:4.6.1.2)
Short name:
RETGC-2
Alternative name(s):
Guanylate cyclase 2F, retinal
Guanylate cyclase F
Short name:
GC-F
Rod outer segment membrane guanylate cyclase 2
Short name:
ROS-GC2
Gene namesi
Name:GUCY2F
Synonyms:GUC2F, RETGC2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

HGNCiHGNC:4691. GUCY2F.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini51 – 467ExtracellularSequence analysisAdd BLAST417
Transmembranei468 – 490HelicalSequence analysisAdd BLAST23
Topological domaini491 – 1108CytoplasmicSequence analysisAdd BLAST618

GO - Cellular componenti

  • guanylate cyclase complex, soluble Source: GO_Central
  • integral component of plasma membrane Source: ProtInc
  • nuclear outer membrane Source: ProtInc
  • photoreceptor disc membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi2986.
OpenTargetsiENSG00000101890.
PharmGKBiPA29071.

Polymorphism and mutation databases

BioMutaiGUCY2F.
DMDMi311033391.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 50Sequence analysisAdd BLAST50
ChainiPRO_000001238651 – 1108Retinal guanylyl cyclase 2Add BLAST1058

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi104 ↔ 132By similarity
Disulfide bondi452InterchainBy similarity
Disulfide bondi460InterchainBy similarity

Post-translational modificationi

There are 9 conserved cysteine residues in sensory guanylate cyclases, 6 in the extracellular domain, which may be involved in intra- or interchain disulfide bonds.

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiP51841.
PaxDbiP51841.
PeptideAtlasiP51841.
PRIDEiP51841.

PTM databases

iPTMnetiP51841.
PhosphoSitePlusiP51841.

Expressioni

Tissue specificityi

Retina. Localized exclusively in the outer nuclear layer and inner segments of the rod and cone photoreceptor cells.

Gene expression databases

BgeeiENSG00000101890.
CleanExiHS_GUCY2F.
GenevisibleiP51841. HS.

Organism-specific databases

HPAiHPA000734.
HPA027473.

Interactioni

Protein-protein interaction databases

BioGridi109241. 3 interactors.
IntActiP51841. 2 interactors.
MINTiMINT-7970335.
STRINGi9606.ENSP00000218006.

Structurei

3D structure databases

ProteinModelPortaliP51841.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini532 – 812Protein kinasePROSITE-ProRule annotationAdd BLAST281
Domaini884 – 1014Guanylate cyclasePROSITE-ProRule annotationAdd BLAST131

Domaini

The protein kinase domain is predicted to be catalytically inactive.

Sequence similaritiesi

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation
Contains 1 guanylate cyclase domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1023. Eukaryota.
COG2114. LUCA.
GeneTreeiENSGT00760000118959.
HOGENOMiHOG000293307.
HOVERGENiHBG098487.
InParanoidiP51841.
KOiK12322.
OMAiQVWTLPY.
OrthoDBiEOG091G00VM.
PhylomeDBiP51841.
TreeFamiTF106338.

Family and domain databases

Gene3Di3.30.70.1230. 1 hit.
InterProiIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR001828. ANF_lig-bd_rcpt.
IPR033484. GUCY2F.
IPR011645. HNOB_dom_associated.
IPR011009. Kinase-like_dom.
IPR029787. Nucleotide_cyclase.
IPR028082. Peripla_BP_I.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PANTHERiPTHR11920:SF349. PTHR11920:SF349. 1 hit.
PfamiPF01094. ANF_receptor. 1 hit.
PF00211. Guanylate_cyc. 1 hit.
PF07701. HNOBA. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SMARTiSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
SSF55073. SSF55073. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P51841-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLGLGRFSR LVLWFAAFRK LLGHHGLASA KFLWCLCLLS VMSLPQQVWT
60 70 80 90 100
LPYKIGVVGP WACDSLFSKA LPEVAARLAI ERINRDPSFD LSYSFEYVIL
110 120 130 140 150
NEDCQTSRAL SSFISHHQMA SGFIGPTNPG YCEAASLLGN SWDKGIFSWA
160 170 180 190 200
CVNYELDNKI SYPTFSRTLP SPIRVLVTVM KYFQWAHAGV ISSDEDIWVH
210 220 230 240 250
TANRVASALR SHGLPVGVVL TTGQDSQSMR KALQRIHQAD RIRIIIMCMH
260 270 280 290 300
SALIGGETQM HLLECAHDLK MTDGTYVFVP YDALLYSLPY KHTPYRVLRN
310 320 330 340 350
NPKLREAYDA VLTITVESQE KTFYQAFTEA AARGEIPEKL EFDQVSPLFG
360 370 380 390 400
TIYNSIYFIA QAMNNAMKEN GQAGAASLVQ HSRNMQFHGF NQLMRTDSNG
410 420 430 440 450
NGISEYVILD TNLKEWELHS TYTVDMEMEL LRFGGTPIHF PGGRPPRADA
460 470 480 490 500
KCWFAEGKIC HGGIDPAFAM MVCLTLLIAL LSINGFAYFI RRRINKIQLI
510 520 530 540 550
KGPNRILLTL EDVTFINPHF GSKRGSRASV SFQITSEVQS GRSPRLSFSS
560 570 580 590 600
GSLTPATYEN SNIAIYEGDW VWLKKFSLGD FGDLKSIKSR ASDVFEMMKD
610 620 630 640 650
LRHENINPLL GFFYDSGMFA IVTEFCSRGS LEDILTNQDV KLDWMFKSSL
660 670 680 690 700
LLDLIKGMKY LHHREFVHGR LKSRNCVVDG RFVLKVTDYG FNDILEMLRL
710 720 730 740 750
SEEESSMEEL LWTAPELLRA PRGSRLGSFA GDVYSFAIIM QEVMVRGTPF
760 770 780 790 800
CMMDLPAQEI INRLKKPPPV YRPVVPPEHA PPECLQLMKQ CWAEAAEQRP
810 820 830 840 850
TFDEIFNQFK TFNKGKKTNI IDSMLRMLEQ YSSNLEDLIR ERTEELEIEK
860 870 880 890 900
QKTEKLLTQM LPPSVAESLK KGCTVEPEGF DLVTLYFSDI VGFTTISAMS
910 920 930 940 950
EPIEVVDLLN DLYTLFDAII GSHDVYKVET IGDAYMVASG LPKRNGSRHA
960 970 980 990 1000
AEIANMSLDI LSSVGTFKMR HMPEVPVRIR IGLHSGPVVA GVVGLTMPRY
1010 1020 1030 1040 1050
CLFGDTVNTA SRMESTGLPY RIHVSLSTVT ILQNLSEGYE VELRGRTELK
1060 1070 1080 1090 1100
GKGTEETFWL IGKKGFMKPL PVPPPVDKDG QVGHGLQPVE IAAFQRRKAE

RQLVRNKP
Length:1,108
Mass (Da):124,850
Last modified:November 2, 2010 - v2
Checksum:iB047BBF1A3C009F9
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03641910R → P in a breast cancer sample; somatic mutation. 1 PublicationCorresponds to variant rs755991142dbSNPEnsembl.1
Natural variantiVAR_04223340S → C.1 PublicationCorresponds to variant rs34228145dbSNPEnsembl.1
Natural variantiVAR_042234160I → N.1 PublicationCorresponds to variant rs33971675dbSNPEnsembl.1
Natural variantiVAR_042235230R → W.1 PublicationCorresponds to variant rs33973457dbSNPEnsembl.1
Natural variantiVAR_009136284L → P.1 PublicationCorresponds to variant rs12008095dbSNPEnsembl.1
Natural variantiVAR_009137296R → Q.1 PublicationCorresponds to variant rs502209dbSNPEnsembl.1
Natural variantiVAR_042236305R → Q.1 PublicationCorresponds to variant rs55966326dbSNPEnsembl.1
Natural variantiVAR_030633308Y → C.1 PublicationCorresponds to variant rs16985750dbSNPEnsembl.1
Natural variantiVAR_030634380Q → H.1 PublicationCorresponds to variant rs2272925dbSNPEnsembl.1
Natural variantiVAR_042237434G → R.1 PublicationCorresponds to variant rs56293008dbSNPEnsembl.1
Natural variantiVAR_042238568G → D in a glioblastoma multiforme sample; somatic mutation. 1 PublicationCorresponds to variant rs779221554dbSNPEnsembl.1
Natural variantiVAR_030635628R → Q.1 PublicationCorresponds to variant rs7883913dbSNPEnsembl.1
Natural variantiVAR_042239677V → L.1 PublicationCorresponds to variant rs35474112dbSNPEnsembl.1
Natural variantiVAR_042240794E → K.1 PublicationCorresponds to variant rs35726803dbSNPEnsembl.1
Natural variantiVAR_069424872G → D.1 PublicationCorresponds to variant rs148663380dbSNPEnsembl.1
Natural variantiVAR_0422411010A → V.1 PublicationCorresponds to variant rs55735218dbSNPEnsembl.1
Natural variantiVAR_0422421052K → R in a lung adenocarcinoma sample; somatic mutation. 1 Publication1
Natural variantiVAR_0422431055E → D in a lung squamous cell carcinoma sample; somatic mutation. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L37378 mRNA. Translation: AAA74451.1.
AL031387 Genomic DNA. Translation: CAB41303.1.
CCDSiCCDS14545.1.
PIRiI59385.
RefSeqiNP_001513.2. NM_001522.2.
UniGeneiHs.123074.

Genome annotation databases

EnsembliENST00000218006; ENSP00000218006; ENSG00000101890.
GeneIDi2986.
KEGGihsa:2986.
UCSCiuc065aqx.1. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Mutations of the GUCY2F gene

Retina International's Scientific Newsletter

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L37378 mRNA. Translation: AAA74451.1.
AL031387 Genomic DNA. Translation: CAB41303.1.
CCDSiCCDS14545.1.
PIRiI59385.
RefSeqiNP_001513.2. NM_001522.2.
UniGeneiHs.123074.

3D structure databases

ProteinModelPortaliP51841.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109241. 3 interactors.
IntActiP51841. 2 interactors.
MINTiMINT-7970335.
STRINGi9606.ENSP00000218006.

PTM databases

iPTMnetiP51841.
PhosphoSitePlusiP51841.

Polymorphism and mutation databases

BioMutaiGUCY2F.
DMDMi311033391.

Proteomic databases

MaxQBiP51841.
PaxDbiP51841.
PeptideAtlasiP51841.
PRIDEiP51841.

Protocols and materials databases

DNASUi2986.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000218006; ENSP00000218006; ENSG00000101890.
GeneIDi2986.
KEGGihsa:2986.
UCSCiuc065aqx.1. human.

Organism-specific databases

CTDi2986.
DisGeNETi2986.
GeneCardsiGUCY2F.
H-InvDBHIX0056276.
HGNCiHGNC:4691. GUCY2F.
HPAiHPA000734.
HPA027473.
MIMi300041. gene.
neXtProtiNX_P51841.
OpenTargetsiENSG00000101890.
PharmGKBiPA29071.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1023. Eukaryota.
COG2114. LUCA.
GeneTreeiENSGT00760000118959.
HOGENOMiHOG000293307.
HOVERGENiHBG098487.
InParanoidiP51841.
KOiK12322.
OMAiQVWTLPY.
OrthoDBiEOG091G00VM.
PhylomeDBiP51841.
TreeFamiTF106338.

Enzyme and pathway databases

BioCyciZFISH:HS02314-MONOMER.
BRENDAi4.6.1.2. 2681.
ReactomeiR-HSA-2514859. Inactivation, recovery and regulation of the phototransduction cascade.

Miscellaneous databases

GenomeRNAii2986.
PROiP51841.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000101890.
CleanExiHS_GUCY2F.
GenevisibleiP51841. HS.

Family and domain databases

Gene3Di3.30.70.1230. 1 hit.
InterProiIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR001828. ANF_lig-bd_rcpt.
IPR033484. GUCY2F.
IPR011645. HNOB_dom_associated.
IPR011009. Kinase-like_dom.
IPR029787. Nucleotide_cyclase.
IPR028082. Peripla_BP_I.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PANTHERiPTHR11920:SF349. PTHR11920:SF349. 1 hit.
PfamiPF01094. ANF_receptor. 1 hit.
PF00211. Guanylate_cyc. 1 hit.
PF07701. HNOBA. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SMARTiSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
SSF55073. SSF55073. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGUC2F_HUMAN
AccessioniPrimary (citable) accession number: P51841
Secondary accession number(s): Q9UJF1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 2, 2010
Last modified: November 2, 2016
This is version 163 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.