P51840 (GUC2E_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanylyl cyclase GC-E
EC=4.6.1.2

Alternative name(s):
Guanylate cyclase 2E

Gene names

Name:	Gucy2e
Synonyms:	Guc2e

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	1108 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	GTP = 3',5'-cyclic GMP + diphosphate.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Expressed in the eye and the pineal gland.
Post-translational modification	There are 9 conserved cysteine residues in sensory guanylate cyclases, 6 in the extracellular domain, which may be involved in intra- or interchain disulfide bonds.
Sequence similarities	Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Contains 1 guanylate cyclase domain. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Sensory transduction Vision cGMP biosynthesis
Cellular component	Membrane
Domain	Signal Transmembrane Transmembrane helix
Ligand	GTP-binding Nucleotide-binding
Molecular function	Lyase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from electronic annotation. Source: InterPro visual perception Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Traceable author statement Ref.1. Source: RGD plasma membrane Inferred from direct assay PubMed 9153227. Source: RGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: InterPro GTP binding Inferred from electronic annotation. Source: UniProtKB-KW guanylate cyclase activity Inferred from direct assay Ref.1. Source: RGD protein kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 54	54	Potential
	Chain	55 – 1108	1054	Guanylyl cyclase GC-E		PRO_0000012384
Regions
	Topological domain	55 – 465	411	Extracellular Potential
	Transmembrane	466 – 490	25	Helical; Potential
	Topological domain	491 – 1108	618	Cytoplasmic Potential
	Domain	520 – 811	292	Protein kinase
	Domain	883 – 1013	131	Guanylate cyclase
Amino acid modifications
	Glycosylation	300	1	N-linked (GlcNAc...) Potential
	Disulfide bond	108 ↔ 136		By similarity
	Disulfide bond	452		Interchain By similarity
	Disulfide bond	460		Interchain By similarity

Sequences

	Sequence		Length	Mass (Da)
	P51840 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 4FA1BE9325A2A979	FASTA	1,108	120,801
	10 20 30 40 50 60 MSAWLLPAGG FPGAGFCIPA WQSRSSLSRV LRWPGPGLPG LLLLLLLPSP SAFSAVFKVG 70 80 90 100 110 120 VLGPWACDPI FARARPDLAA RLATDRLNRD LALDGGPWFE VTLLPEPCLT PGSLGAVSSA 130 140 150 160 170 180 LTRVSGLVGP VNPAACRPAE LLAQEAGVAL VPWGCPGTRA AGTTAPAVTP AADALYVLLK 190 200 210 220 230 240 AFRWARVALI TAPQDLWVEA GRALSTALRA RGLPVALVTS MVPSDLSGAR EALRRIRDGP 250 260 270 280 290 300 RVRVVIMVMH SVLLGGEEQR YLLEAAEELG LTDGSLVFLP FDTLHYALSP GPEALAAFVN 310 320 330 340 350 360 SSKLRRAHDA VLTLTRRCPP GGSVQDSLRR AQEHQELPLD LDLKQVSPLF GTIYDAVFLL 370 380 390 400 410 420 AGGVTRARAA VGGGWVSGAS VARQMREAQV FGFCGILGRT EEPSFVLLDT DAAGERLFTT 430 440 450 460 470 480 HLLDPVLGSL RSAGTPVHFP RGAPAPGPDP SCWFDPDVIC NGGVEPGLVF VGFLLVIVVG 490 500 510 520 530 540 LTGAFLAHYL RHRLLHMQMV SGPNKIILTL EDVTFLHPQG GSSRKVAQGS RSSLATRSTS 550 560 570 580 590 600 DIRSVPSQPQ ESTNIGLYEG DWVWLKKFPG EHHMAIRPAT KMAFSKLREL RHENVALYLG 610 620 630 640 650 660 LFLAGTADSP ATPGEGILAV VSEHCARGSL HDLLAQRDIK LDWMFKSSLL LDLIKGMRYL 670 680 690 700 710 720 HHRGVAHGRL KSRNCVVDGR FVLKVTDHGH GRLLEAQRVL PEPPSAEDQL WTAPELLRDP 730 740 750 760 770 780 ALERRGTLAG DVFSLGIIMQ EVVCRSTPYA MLELTPEEVI QRVRSPPPLC RPLVSMDQAP 790 800 810 820 830 840 MECIQLMAQC WAEHPELRPS MDLTFDLFKG INKGRKTNII DSMLRMLEQY SSNLEDLIRE 850 860 870 880 890 900 RTEELEQEKQ KTDRLLTQML PPSVAEALKM GTSVEPEYFE EVTLYFSDIV GFTTISAMSE 910 920 930 940 950 960 PIEVVDLLND LYTLFDAIIG SHDVYKVETI GDAYMVASGL PQRNGQRHAA EIANMSLDIL 970 980 990 1000 1010 1020 SAVGSFRMRH MPEVPVRIRI GLHSGPCVAG VVGLTMPRYC LFGDTVNTAS RMESTGLPYR 1030 1040 1050 1060 1070 1080 IHVNMSTVRI LRALDQGFQM ECRGRTELKG KGVEDTYWLV GRVGFNKPIP KPPDLQPGAS 1090 1100 NHGISLQEIP PERRKKLEKA RPGQFTGK

References

[1]	"Two membrane forms of guanylyl cyclase found in the eye." Yang R.-B., Foster D.C., Garbers D.L., Fuelle H.-J. Proc. Natl. Acad. Sci. U.S.A. 92:602-606(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Eye.
[2]	Lubec G., Kang S.U. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 203-209, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L36029 mRNA. Translation: AAA65510.1.
IPI	IPI00326408.
PIR	A55915.
RefSeq	NP_077356.1. NM_024380.1.
UniGene	Rn.10060.
3D structure databases
ProteinModelPortal	P51840.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000010790.
PTM databases
PhosphoSite	P51840.
Proteomic databases
PaxDb	P51840.
PRIDE	P51840.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	79222.
KEGG	rno:79222.
UCSC	RGD:620438. rat.
Organism-specific databases
CTD	14919.
RGD	620438. Gucy2e.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000293307.
HOVERGEN	HBG098487.
InParanoid	P51840.
KO	K12321.
OMA	LTLTRHC.
OrthoDB	EOG4XD3QC.
Gene expression databases
Genevestigator	P51840.
GermOnline	ENSRNOG00000007931. Rattus norvegicus.
Family and domain databases
Gene3D	3.30.70.1230. 1 hit.
InterPro	IPR001054. A/G_cyclase. IPR018297. A/G_cyclase_CS. IPR001828. ANF_lig-bd_rcpt. IPR011645. Haem_no_assoc-bd. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. [Graphical view]
Pfam	PF01094. ANF_receptor. 1 hit. PF00211. Guanylate_cyc. 1 hit. PF07701. HNOBA. 1 hit. PF07714. Pkinase_Tyr. 1 hit. [Graphical view]
SMART	SM00044. CYCc. 1 hit. [Graphical view]
SUPFAM	SSF55073. A/G_cyclase. 1 hit. SSF56112. Kinase_like. 1 hit.
PROSITE	PS00452. GUANYLATE_CYCLASE_1. 1 hit. PS50125. GUANYLATE_CYCLASE_2. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	614644.

Entry information

Entry name

GUC2E_RAT

Accession

Primary (citable) accession number: P51840

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 117 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program