P51837 (RNC_COXBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease 3
EC=3.1.26.3

Alternative name(s):
Ribonuclease III
Short name=RNase III

Gene names

Name:	rnc
Ordered Locus Names:	CBU_1503

Organism

Coxiella burnetii (strain RSA 493 / Nine Mile phase I) [Reference proteome] [HAMAP]

Taxonomic identifier

227377 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella › Coxiella burnetii

Protein attributes

Sequence length	233 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon By similarity. HAMAP-Rule MF_00104
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP-Rule MF_00104
Cofactor	Mg2+ By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm HAMAP-Rule MF_00104.
Sequence similarities	Contains 1 DRBM (double-stranded RNA-binding) domain. Contains 1 RNase III domain.

Ontologies

Keywords
Biological process	mRNA processing rRNA processing tRNA processing
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding RNA-binding rRNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA processing Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC rRNA catabolic process Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: HAMAP tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	double-stranded RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease III activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 233	233	Ribonuclease 3 HAMAP-Rule MF_00104		PRO_0000180394
Regions
	Domain	4 – 126	123	RNase III
	Domain	153 – 222	70	DRBM
Sites
	Active site	43	1	Potential
	Active site	115	1	By similarity
	Metal binding	39	1	Magnesium By similarity
	Metal binding	112	1	Magnesium By similarity
	Metal binding	115	1	Magnesium By similarity
Experimental info
	Sequence conflict	116	1	A → T in AAA69690. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P51837 [UniParc]. Last modified May 9, 2003. Version 2. Checksum: 1A11CB5FD960784F	FASTA	233	26,199
	10 20 30 40 50 60 MNHLNKLMER LGHQFNNLEL LKIALTHCSS GADNNERLEF LGDSVLGFII ASELYQRRPQ 70 80 90 100 110 120 AREGDLSRMR ASMVNGDELA QMSTKLGINE YLQLGVGEQK SGGKRRRSIL ADALEAIVGA 130 140 150 160 170 180 IYIDAGLETC RRCVLNWYGE RVDDLSKLSP KKDAKSLLQE WLQARRLPLP TYEVKITGEA 190 200 210 220 230 HAQTFTVNCY VKGLPHKTEG VNTTRRRAEQ IAAKRFLELL DDGKGDGITE RDQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the rnc locus of Coxiella burnetii." Zuber M., Hoover T.A., Powell B.S., Court D.L. Mol. Microbiol. 14:291-300(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CB9MIC7.
[2]	"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RSA 493 / Nine Mile phase I.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L27436 Genomic DNA. Translation: AAA69690.1. AE016828 Genomic DNA. Translation: AAO91000.1.
PIR	S60767.
RefSeq	NP_820486.1. NC_002971.3.
3D structure databases
ProteinModelPortal	P51837.
ModBase	Search...
Protein-protein interaction databases
STRING	227377.CBU_1503.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO91000; AAO91000; CBU_1503.
GeneID	1209413.
KEGG	cbu:CBU_1503.
PATRIC	17931771. VBICoxBur82552_1505.
Phylogenomic databases
eggNOG	COG0571.
HOGENOM	HOG000246809.
KO	K03685.
OMA	RACELPR.
ProtClustDB	PRK00102.
Enzyme and pathway databases
BioCyc	CBUR227377:GJ7S-1488-MONOMER.
Family and domain databases
Gene3D	1.10.1520.10. 1 hit. 3.30.160.20. 1 hit.
HAMAP	MF_00104. RNase_III.
InterPro	IPR001159. Ds-RNA-bd. IPR014720. dsRNA-bd-like_dom. IPR011907. RNase_III. IPR000999. RNase_III_dom. [Graphical view]
PANTHER	PTHR11207. PTHR11207. 1 hit.
Pfam	PF00035. dsrm. 1 hit. PF00636. Ribonuclease_3. 1 hit. [Graphical view]
SMART	SM00358. DSRM. 1 hit. SM00535. RIBOc. 1 hit. [Graphical view]
SUPFAM	SSF69065. RNase_III. 1 hit.
TIGRFAMs	TIGR02191. RNaseIII. 1 hit.
PROSITE	PS50137. DS_RBD. 1 hit. PS00517. RNASE_3_1. 1 hit. PS50142. RNASE_3_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNC_COXBU

Accession

Primary (citable) accession number: P51837

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	May 9, 2003
Last modified:	May 1, 2013
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program