P51834 (SMC_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chromosome partition protein Smc

Gene names

Name:	smc
Synonyms:	ylqA
Ordered Locus Names:	BSU15940

Organism

Bacillus subtilis (strain 168) [Reference proteome] [HAMAP]

Taxonomic identifier

224308 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group › Bacillus subtilis › Bacillus subtilis subsp. subtilis

Protein attributes

Sequence length	1186 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for chromosome condensation and partitioning. Ref.5 Ref.6
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. Note: Probably associated with the nucleoid. Ref.5
Domain	Contains large globular domains required for ATP hydrolysis at each terminus and a third globular domain forming a flexible hinge near the middle of the molecule. These domains are separated by coiled-coil structures By similarity. HAMAP-Rule MF_01894
Disruption phenotype	Mutants produce anucleate cells and show defects in nucleoid structure and in chromosome partitioning. Ref.5 Ref.6
Sequence similarities	Belongs to the SMC family.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Coiled coil
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA recombination Inferred from electronic annotation. Source: InterPro DNA repair Inferred from electronic annotation. Source: InterPro DNA replication Inferred from electronic annotation. Source: HAMAP chromosome condensation Inferred from electronic annotation. Source: InterPro sister chromatid cohesion Inferred from electronic annotation. Source: InterPro
Cellular_component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		3	EBI-2121372,EBI-2121372
scpA	P35154	6	EBI-2121372,EBI-2121359
scpB	P35155	2	EBI-2121372,EBI-2121445

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1186

1186

Chromosome partition protein Smc HAMAP-Rule MF_01894

PRO_0000119028

Regions

Nucleotide binding

32 – 39

8

ATP By similarity

Coiled coil

167 – 206

40

Potential

Coiled coil

259 – 481

223

Potential

Coiled coil

672 – 864

193

Potential

Coiled coil

893 – 943

51

Potential

Coiled coil

990 – 1029

40

Potential

Experimental info

Sequence conflict

50

1

E → G in BAA10977. Ref.1

Sequence conflict

162

1

E → G in BAA10977. Ref.1

Sequence conflict

175

1

K → E in BAA10977. Ref.1

Sequence conflict

178

1

E → G in BAA10977. Ref.1

Sequence conflict

192

1

E → G in BAA10977. Ref.1

Sequence conflict

228

1

A → P in BAA10977. Ref.1

Sequence conflict

264

1

A → P in BAA10977. Ref.1

Sequence conflict

271

1

D → G in BAA10977. Ref.1

Sequence conflict

309

1

E → D in BAA10977. Ref.1

Sequence conflict

336 – 342

7

KEELSKQ → TRRAFEA in BAA10977. Ref.1

Sequence conflict

365

1

Q → H in BAA10977. Ref.1

Sequence conflict

438

1

E → K in BAA10977. Ref.1

Sequence conflict

444

1

I → F in BAA10977. Ref.1

Sequence conflict

475

1

A → P in BAA10977. Ref.1

Sequence conflict

494

1

E → D in BAA10977. Ref.1

Sequence conflict

515

1

E → D in BAA10977. Ref.1

Sequence conflict

542

1

L → V in BAA10977. Ref.1

Sequence conflict

546

1

A → P in BAA10977. Ref.1

Sequence conflict

586 – 600

15

QSRDA…HSSFL → SKPLRGNSGPAFIISF in BAA10977. Ref.1

Sequence conflict

623 – 631

9

TVLITEDLK → NRSDYRGLKG in BAA10977. Ref.1

Sequence conflict

664

1

A → S in BAA10977. Ref.1

Sequence conflict

676

1

S → T in BAA10977. Ref.1

Sequence conflict

680

1

E → G in BAA10977. Ref.1

Sequence conflict

694

1

A → S in BAA10977. Ref.1

Sequence conflict

701

1

K → Q in BAA10977. Ref.1

Sequence conflict

726

1

L → V in BAA10977. Ref.1

Sequence conflict

738 – 740

3

LQV → PQF in BAA10977. Ref.1

Secondary structure

1

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1186

Helix Strand Turn

Details...

Beta strand

6 – 14

9

Beta strand

24 – 30

7

Helix

39 – 48

10

Beta strand

83 – 89

7

Beta strand

100 – 107

8

Beta strand

112 – 114

3

Helix

122 – 125

4

Helix

126 – 128

3

Beta strand

139 – 142

4

Helix

143 – 151

9

Helix

154 – 164

11

Helix

168 – 197

30

Helix

996 – 1052

57

Beta strand

1056 – 1059

4

Beta strand

1062 – 1066

5

Helix

1067 – 1069

3

Beta strand

1074 – 1077

4

Beta strand

1079 – 1081

3

Beta strand

1083 – 1089

7

Helix

1093 – 1108

16

Beta strand

1114 – 1118

5

Turn

1119 – 1122

4

Helix

1126 – 1137

12

Beta strand

1139 – 1142

4

Beta strand

1144 – 1147

4

Helix

1151 – 1156

6

Beta strand

1160 – 1164

5

Beta strand

1171 – 1174

4

Sequences

	Sequence		Length	Mass (Da)
	P51834 [UniParc]. Last modified June 16, 2009. Version 3. Checksum: ACC5D1A170453212	FASTA	1,186	135,513
	10 20 30 40 50 60 MFLKRLDVIG FKSFAERISV DFVKGVTAVV GPNGSGKSNI TDAIRWVLGE QSARSLRGGK 70 80 90 100 110 120 MEDIIFAGSD SRKRLNLAEV TLTLDNDDHF LPIDFHEVSV TRRVYRSGES EFLINNQPCR 130 140 150 160 170 180 LKDIIDLFMD SGLGKEAFSI ISQGKVEEIL SSKAEDRRSI FEEAAGVLKY KTRKKKAENK 190 200 210 220 230 240 LFETQDNLNR VEDILHELEG QVEPLKIQAS IAKDYLEKKK ELEHVEIALT AYDIEELHGK 250 260 270 280 290 300 WSTLKEKVQM AKEEELAESS AISAKEAKIE DTRDKIQALD ESVDELQQVL LVTSEELEKL 310 320 330 340 350 360 EGRKEVLKER KKNAVQNQEQ LEEAIVQFQQ KETVLKEELS KQEAVFETLQ AEVKQLRAQV 370 380 390 400 410 420 KEKQQALSLH NENVEEKIEQ LKSDYFELLN SQASIRNELQ LLDDQMSQSA VTLQRLADNN 430 440 450 460 470 480 EKHLQERHDI SARKAACETE FARIEQEIHS QVGAYRDMQT KYEQKKRQYE KNESALYQAY 490 500 510 520 530 540 QYVQQARSKK DMLETMQGDF SGFYQGVKEV LKAKERLGGI RGAVLELIST EQKYETAIEI 550 560 570 580 590 600 ALGASAQHVV TDDEQSARKA IQYLKQNSFG RATFLPLSVI RDRQLQSRDA ETAARHSSFL 610 620 630 640 650 660 GVASELVTFD PAYRSVIQNL LGTVLITEDL KGANELAKLL GHRYRIVTLE GDVVNPGGSM 670 680 690 700 710 720 TGGAVKKKNN SLLGRSRELE DVTKRLAEME EKTALLEQEV KTLKHSIQDM EKKLADLRET 730 740 750 760 770 780 GEGLRLKQQD VKGQLYELQV AEKNINTHLE LYDQEKSALS ESDEERKVRK RKLEEELSAV 790 800 810 820 830 840 SEKMKQLEED IDRLTKQKQT QSSTKESLSN ELTELKIAAA KKEQACKGEE DNLARLKKEL 850 860 870 880 890 900 TETELALKEA KEDLSFLTSE MSSSTSGEEK LEEAAKHKLN DKTKTIELIA LRRDQRIKLQ 910 920 930 940 950 960 HGLDTYEREL KEMKRLYKQK TTLLKDEEVK LGRMEVELDN LLQYLREEYS LSFEGAKEKY 970 980 990 1000 1010 1020 QLETDPEEAR KRVKLIKLAI EELGTVNLGS IDEFERVNER YKFLSEQKED LTEAKNTLFQ 1030 1040 1050 1060 1070 1080 VIEEMDEEMT KRFNDTFVQI RSHFDQVFRS LFGGGRAELR LTDPNDLLHS GVEIIAQPPG 1090 1100 1110 1120 1130 1140 KKLQNLNLLS GGERALTAIA LLFSILKVRP VPFCVLDEVE AALDEANVFR FAQYLKKYSS 1150 1160 1170 1180 DTQFIVITHR KGTMEEADVL YGVTMQESGV SKVISVKLEE TKEFVQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The effect of Srb, a homologue of the mammalian SRP receptor alpha-subunit, on Bacillus subtilis growth and protein translocation." Oguro A., Kakeshita H., Takamatsu H., Nakamura K., Yamane K. Gene 172:17-24(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION TO 236 AND 284.
[4]	"srb: a Bacillus subtilis gene encoding a homologue of the alpha-subunit of the mammalian signal recognition particle receptor." Oguro A., Kakeshita H., Honda K., Takamatsu H., Nakamura K., Yamane K. DNA Res. 2:95-100(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1171-1186. Strain: 168.
[5]	"Characterization of a prokaryotic SMC protein involved in chromosome partitioning." Britton R.A., Lin D.C., Grossman A.D. Genes Dev. 12:1254-1259(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE. Strain: 168 / JH642.
[6]	"A Bacillus subtilis gene-encoding protein homologous to eukaryotic SMC motor protein is necessary for chromosome partition." Moriya S., Tsujikawa E., Hassan A.K., Asai K., Kodama T., Ogasawara N. Mol. Microbiol. 29:179-187(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE. Strain: 168.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D64116 Genomic DNA. Translation: BAA10977.1.
AL009126 Genomic DNA. Translation: CAB13467.2.
D49781 Genomic DNA. Translation: BAA08615.1.

PIR

G69708.

RefSeq

NP_389476.2. NC_000964.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3ZGX	X-ray	3.40	A/B	1-1186	[»]

ProteinModelPortal

P51834.

SMR

P51834. Positions 1-197, 485-685, 1029-1186.

ModBase

Search...

Protein-protein interaction databases

IntAct

P51834. 17 interactions.

STRING

224308.BSU15940.

Proteomic databases

PaxDb

P51834.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

CAB13467; CAB13467; BSU15940.

GeneID

938085.

KEGG

bsu:BSU15940.

PATRIC

18974993. VBIBacSub10457_1688.

Organism-specific databases

GenoList

BSU15940. [Micado]

Phylogenomic databases

eggNOG

COG1196.

HOGENOM

HOG000036392.

KO

K03529.

ProtClustDB

CLSK2765274.

Enzyme and pathway databases

BioCyc

BSUB:BSU15940-MONOMER.

Family and domain databases

HAMAP

MF_01894. Smc_prok.

InterPro

IPR003395. RecF/RecN/SMC.
IPR024704. SMC.
IPR010935. SMC_hinge.
IPR011890. SMC_prok.
[Graphical view]

Pfam

PF06470. SMC_hinge. 1 hit.
PF02463. SMC_N. 1 hit.
[Graphical view]

PIRSF

PIRSF005719. SMC. 1 hit.

SMART

SM00968. SMC_hinge. 1 hit.
[Graphical view]

SUPFAM

SSF75553. SMC_hinge. 1 hit.

TIGRFAMs

TIGR02168. SMC_prok_B. 1 hit.

ProtoNet

Search...

Entry information

Entry name

SMC_BACSU

Accession

Primary (citable) accession number: P51834
Secondary accession number(s): O31735

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	June 16, 2009
Last modified:	May 1, 2013
This is version 96 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program