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Protein

AF4/FMR2 family member 3

Gene

Aff3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Putative transcription activator that may function in lymphoid development and oncogenesis.

GO - Molecular functioni

  • double-stranded DNA binding Source: MGI
  • transcription factor activity, sequence-specific DNA binding Source: MGI

GO - Biological processi

  • embryonic hindlimb morphogenesis Source: BHF-UCL
  • response to tumor necrosis factor Source: MGI
  • transcription, DNA-templated Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
AF4/FMR2 family member 3
Alternative name(s):
Lymphoid nuclear protein related to AF4
Short name:
Protein LAF-4
Gene namesi
Name:Aff3
Synonyms:Laf4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:106927. Aff3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002159171 – 1254AF4/FMR2 family member 3Add BLAST1254

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei782PhosphoserineCombined sources1
Modified residuei908PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP51827.
PRIDEiP51827.

PTM databases

iPTMnetiP51827.
PhosphoSitePlusiP51827.

Expressioni

Tissue specificityi

Highest levels found in lymphoid tissues, lower levels in brain and lung.

Gene expression databases

CleanExiMM_AFF3.

Interactioni

Protein-protein interaction databases

IntActiP51827. 2 interactors.
STRINGi10090.ENSMUSP00000044128.

Structurei

3D structure databases

ProteinModelPortaliP51827.
SMRiP51827.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi431 – 490Ser-richAdd BLAST60
Compositional biasi696 – 752Ser-richAdd BLAST57
Compositional biasi1125 – 1177Ser-richAdd BLAST53

Sequence similaritiesi

Belongs to the AF4 family.Curated

Phylogenomic databases

eggNOGiENOG410IGJB. Eukaryota.
ENOG410XRXU. LUCA.
HOGENOMiHOG000246991.
HOVERGENiHBG004189.
InParanoidiP51827.
PhylomeDBiP51827.

Family and domain databases

InterProiIPR007797. TF_AF4/FMR2.
[Graphical view]
PANTHERiPTHR10528. PTHR10528. 1 hit.
PfamiPF05110. AF-4. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P51827-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDSFDLALLQ EWDLESLWGE DILSQRNDSL VLEVQASASR CRSVYEPDRN
60 70 80 90 100
ALRRKERERR SQETQQDSGS FNSGYSLFSE PYKTNKGDEL SNRIQNTLGN
110 120 130 140 150
YDEMKDFLTD RSNQSHLVGV PKPGVPQTPV NKIDEHFGAE SRAQPQPSTV
160 170 180 190 200
CSTASSTPAA VPVQQGKRGA MGWQKAGHPP SDGQQRAAQQ GSLRTLLGDG
210 220 230 240 250
VGRQQTRAKQ VCNMETGLQT QERPPAMAAK HGGSGHCVQN FPPSLASKPS
260 270 280 290 300
LVQQKPTAYV RPMDGQDQAP DESPKLKSST ETAVHCTAYR GVPANKPESA
310 320 330 340 350
RAKAKLAKFS IPKQAEESRS GENNSCVEEI IREMTWLPPL SAIQAPAKVE
360 370 380 390 400
PSKFPFPNKD SQLVSSGHSN PKKADAEPGS PDNGASNTST LEDDLKLSSD
410 420 430 440 450
DEEGEQQAAQ RTALRALADS SVVQQTNCRG SAPSSKGGGS SSSSGGSSSS
460 470 480 490 500
SDSESTSGSD SETESSSSSS ESEGSKPPHC SSPEAEPASS NKWQLDKWLN
510 520 530 540 550
KVNPHKPPIL IQNESHGPER NQYYTPPVKD EGQDCGKLPE ICQASLRDKE
560 570 580 590 600
LKTTCKEEQR PRTANKAPGS KSVKQKSPPA AVAVTAAALP PAVPSAPTES
610 620 630 640 650
APAPTRRSAG KKPTRRTERT SAGDGANCHR PEEPVAPDTL GASVVGPLEP
660 670 680 690 700
PKTRPCGNNR TGHRKELRSS VTCEKRRTRG LSRIVPKSKE FIETESSSSS
710 720 730 740 750
SSSDSDLESE QEEYVLSKAP TTTGSEQRLK EAASSNNNSN SNSSTSRASV
760 770 780 790 800
GSINARTTSD IAKELEEQFY TLVPFGRNEL LSPLKDSDEV RSLWVKIDLT
810 820 830 840 850
LLSRIPEHLS QEPGVLSAPS AKDTDSAPAS HALDAPAEKT LPKSKRKRKC
860 870 880 890 900
DNEDDYREIK KVQGRKESAS RLAASTNNTL SGNHCNVNVN SLAIPINKNE
910 920 930 940 950
KMLRSPTSPL SDTCKHKYAS EDLTSSSRPH GNGLLTSASS NKEPKAESQL
960 970 980 990 1000
QTIAGDLTKA SHNSSENGTL HSKSRPQTEP WSPGSNGHRD CKRQKLIFDD
1010 1020 1030 1040 1050
MPRSADYFMR EAKRMKHKAD AMVEKFGKAL NYAEAALSFI ECGNAMEQGP
1060 1070 1080 1090 1100
MESKSPYTMY SETVELIRYA MRLKTHSGPN ATPEDKQLAA LCYRCLALLY
1110 1120 1130 1140 1150
WRMFRLKRDH AVKYSKALID YFKNSSKAAQ APSPWGSSGK STGSPSPMSP
1160 1170 1180 1190 1200
NPSPASSVGS QGSLSSSSGL SPSTIVSIPQ RIHQMAANHV SITNSILHSY
1210 1220 1230 1240 1250
DYWEMADNLA KENREFFNDL DLLMGPVTLH SSMEHLVQYS QQGLHWLRSS

VHLS
Length:1,254
Mass (Da):136,131
Last modified:January 9, 2007 - v2
Checksum:i240E0CCB568B9478
GO
Isoform 2 (identifier: P51827-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     407-407: Missing.
     564-1254: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.
Show »
Length:562
Mass (Da):60,760
Checksum:i6FB06D927B4807B7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18 – 51WGEDI…PDRNA → CVSITGQEV in AAA98764 (PubMed:8555498).CuratedAdd BLAST34
Sequence conflicti79 – 80SE → RQ in AAA98764 (PubMed:8555498).Curated2
Sequence conflicti84T → S in AAA98764 (PubMed:8555498).Curated1
Sequence conflicti145P → S in AAA98764 (PubMed:8555498).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_022225407Missing in isoform 2. 1 Publication1
Alternative sequenceiVSP_022226564 – 1254Missing in isoform 2. 1 PublicationAdd BLAST691

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC117789 Genomic DNA. No translation available.
AC122026 Genomic DNA. No translation available.
AC132381 Genomic DNA. No translation available.
AC138386 Genomic DNA. No translation available.
BC052061 mRNA. No translation available.
U34361 mRNA. Translation: AAA98764.1.
UniGeneiMm.336679.

Genome annotation databases

UCSCiuc007asu.1. mouse. [P51827-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC117789 Genomic DNA. No translation available.
AC122026 Genomic DNA. No translation available.
AC132381 Genomic DNA. No translation available.
AC138386 Genomic DNA. No translation available.
BC052061 mRNA. No translation available.
U34361 mRNA. Translation: AAA98764.1.
UniGeneiMm.336679.

3D structure databases

ProteinModelPortaliP51827.
SMRiP51827.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP51827. 2 interactors.
STRINGi10090.ENSMUSP00000044128.

PTM databases

iPTMnetiP51827.
PhosphoSitePlusiP51827.

Proteomic databases

PaxDbiP51827.
PRIDEiP51827.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiuc007asu.1. mouse. [P51827-1]

Organism-specific databases

MGIiMGI:106927. Aff3.

Phylogenomic databases

eggNOGiENOG410IGJB. Eukaryota.
ENOG410XRXU. LUCA.
HOGENOMiHOG000246991.
HOVERGENiHBG004189.
InParanoidiP51827.
PhylomeDBiP51827.

Miscellaneous databases

ChiTaRSiAff3. mouse.
PROiP51827.
SOURCEiSearch...

Gene expression databases

CleanExiMM_AFF3.

Family and domain databases

InterProiIPR007797. TF_AF4/FMR2.
[Graphical view]
PANTHERiPTHR10528. PTHR10528. 1 hit.
PfamiPF05110. AF-4. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAFF3_MOUSE
AccessioniPrimary (citable) accession number: P51827
Secondary accession number(s): Q80WS6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 9, 2007
Last modified: November 2, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.