Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P51826 (AFF3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
AF4/FMR2 family member 3
Alternative name(s):
Lymphoid nuclear protein related to AF4
Short name=Protein LAF-4
Gene names
Name:AFF3
Synonyms:LAF4
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1226 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Putative transcription activator that may function in lymphoid development and oncogenesis. Binds, in vitro, to double-stranded DNA.

Subcellular location

Nucleus.

Tissue specificity

Preferentially expressed in lymphoid tissues, highest levels being found in the thymus.

Sequence similarities

Belongs to the AF4 family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P51826-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P51826-2)

The sequence of this isoform differs from the canonical sequence as follows:
     18-18: C → WGEDILNQRNDSLVVEFQSSASRCRS

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12261226AF4/FMR2 family member 3
PRO_0000215916

Regions

Compositional bias413 – 4197Poly-Ser
Compositional bias422 – 43211Poly-Ser
Compositional bias440 – 4456Poly-Ser
Compositional bias670 – 67910Poly-Ser

Amino acid modifications

Modified residue7551Phosphoserine Ref.7
Modified residue8811Phosphoserine Ref.7

Natural variations

Alternative sequence181C → WGEDILNQRNDSLVVEFQSS ASRCRS in isoform 2.
VSP_041120
Natural variant3581S → N. Ref.1 Ref.3 Ref.4
Corresponds to variant rs4851223 [ dbSNP | Ensembl ].
VAR_030805
Natural variant4941N → S.
Corresponds to variant rs1047265 [ dbSNP | Ensembl ].
VAR_030806

Experimental info

Sequence conflict871S → T in AAA98763. Ref.1
Sequence conflict4211S → T in AAA98763. Ref.1
Sequence conflict10301T → YL in AAA98763. Ref.1
Sequence conflict11201M → I in AAA98763. Ref.1
Sequence conflict1125 – 11273SPA → FPG in AAA98763. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 20, 2007. Version 2.
Checksum: 3B51EB475C77A514

FASTA1,226133,476
        10         20         30         40         50         60 
MDSFDLALLQ EWDLESLCVY EPDRNALRRK ERERRNQETQ QDDGTFNSSY SLFSEPYKTN 

        70         80         90        100        110        120 
KGDELSNRIQ NTLGNYDEMK DFLTDRSNQS HLVGVPKPGV PQTPVNKIDE HFVADSRAQN 

       130        140        150        160        170        180 
QPSSICSTTT STPAAVPVQQ SKRGTMGWQK AGHPPSDGQQ RATQQGSLRT LLGDGVGRQQ 

       190        200        210        220        230        240 
PRAKQVCNVE VGLQTQERPP AMAAKHSSSG HCVQNFPPSL ASKPSLVQQK PTAYVRPMDG 

       250        260        270        280        290        300 
QDQAPDESPK LKSSSETSVH CTSYRGVPAS KPEPARAKAK LSKFSIPKQG EESRSGETNS 

       310        320        330        340        350        360 
CVEEIIREMT WLPPLSAIQA PGKVEPTKFP FPNKDSQLVS SGHNNPKKGD AEPESPDSGT 

       370        380        390        400        410        420 
SNTSMLEDDL KLSSDEEENE QQAAQRTALR ALSDSAVVQQ PNCRTSVPSS KGSSSSSSSG 

       430        440        450        460        470        480 
SSSSSSDSES SSGSDSETES SSSESEGSKP PHFSSPEAEP ASSNKWQLDK WLNKVNPHKP 

       490        500        510        520        530        540 
PILIQNESHG SESNQYYNPV KEDVQDCGKV PDVCQPSLRE KEIKSTCKEE QRPRTANKAP 

       550        560        570        580        590        600 
GSKGVKQKSP PAAVAVAVSA AAPPPAVPCA PAENAPAPAR RSAGKKPTRR TERTSAGDGA 

       610        620        630        640        650        660 
NCHRPEEPAA ADALGTSVVV PPEPTKTRPC GNNRASHRKE LRSSVTCEKR RTRGLSRIVP 

       670        680        690        700        710        720 
KSKEFIETES SSSSSSSDSD LESEQEEYPL SKAQTVAASA SSGNDQRLKE AAANGGSGPR 

       730        740        750        760        770        780 
APVGSINART TSDIAKELEE QFYTLVPFGR NELLSPLKDS DEIRSLWVKI DLTLLSRIPE 

       790        800        810        820        830        840 
HLPQEPGVLS APATKDSESA PPSHTSDTPA EKALPKSKRK RKCDNEDDYR EIKKSQGEKD 

       850        860        870        880        890        900 
SSSRLATSTS NTLSANHCNM NINSVAIPIN KNEKMLRSPI SPLSDASKHK YTSEDLTSSS 

       910        920        930        940        950        960 
RPNGNSLFTS ASSSKKPKAD SQLQPHGGDL TKAAHNNSEN IPLHKSRPQT KPWSPGSNGH 

       970        980        990       1000       1010       1020 
RDCKRQKLVF DDMPRSADYF MQEAKRMKHK ADAMVEKFGK ALNYAEAALS FIECGNAMEQ 

      1030       1040       1050       1060       1070       1080 
GPMESKSPYT MYSETVELIR YAMRLKTHSG PNATPEDKQL AALCYRCLAL LYWRMFRLKR 

      1090       1100       1110       1120       1130       1140 
DHAVKYSKAL IDYFKNSSKA AQAPSPWGAS GKSTGTPSPM SPNPSPASSV GSQGSLSNAS 

      1150       1160       1170       1180       1190       1200 
ALSPSTIVSI PQRIHQMAAN HVSITNSILH SYDYWEMADN LAKENREFFN DLDLLMGPVT 

      1210       1220 
LHSSMEHLVQ YSQQGLHWLR NSAHLS 

« Hide

Isoform 2 [UniParc].

Checksum: 73689D7FF83EDF07
Show »

FASTA1,251136,352

References

« Hide 'large scale' references
[1]"LAF-4 encodes a lymphoid nuclear protein with transactivation potential that is homologous to AF-4, the gene fused to MLL in t(4;11) leukemias."
Ma C., Staudt L.M.
Blood 87:734-745(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT ASN-358.
[2]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ASN-358.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT ASN-358.
Tissue: Lymph and Testis.
[5]Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 689-1226.
Tissue: Spleen.
[6]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"Large-scale proteomics analysis of the human kinome."
Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H.
Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-755 AND SER-881, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U34360 mRNA. Translation: AAA98763.1.
AC092667 Genomic DNA. Translation: AAY24278.1.
AC010736 Genomic DNA. Translation: AAY14792.1.
AC073118 Genomic DNA. Translation: AAY24187.1.
AC018690 Genomic DNA. Translation: AAY24315.1.
CH471127 Genomic DNA. Translation: EAX01854.1.
CH471127 Genomic DNA. Translation: EAX01855.1.
CH471127 Genomic DNA. Translation: EAX01856.1.
CH471127 Genomic DNA. Translation: EAX01857.1.
CH471127 Genomic DNA. Translation: EAX01858.1.
CH471127 Genomic DNA. Translation: EAX01859.1.
BC036895 mRNA. Translation: AAH36895.1.
BC136579 mRNA. Translation: AAI36580.1.
BC144266 mRNA. Translation: AAI44267.1.
AB209634 mRNA. Translation: BAD92871.1.
RefSeqNP_001020279.1. NM_001025108.1.
NP_002276.2. NM_002285.2.
XP_005264000.2. XM_005263943.2.
UniGeneHs.444414.

3D structure databases

ProteinModelPortalP51826.
SMRP51826. Positions 23-83.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid110096. 2 interactions.
IntActP51826. 1 interaction.
STRING9606.ENSP00000348793.

PTM databases

PhosphoSiteP51826.

Polymorphism databases

DMDM126302515.

Proteomic databases

PaxDbP51826.
PRIDEP51826.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000317233; ENSP00000317421; ENSG00000144218. [P51826-1]
ENST00000356421; ENSP00000348793; ENSG00000144218. [P51826-2]
ENST00000409236; ENSP00000387207; ENSG00000144218. [P51826-1]
ENST00000409579; ENSP00000386834; ENSG00000144218. [P51826-2]
GeneID3899.
KEGGhsa:3899.
UCSCuc002taf.3. human. [P51826-2]
uc002tag.3. human. [P51826-1]

Organism-specific databases

CTD3899.
GeneCardsGC02M100163.
HGNCHGNC:6473. AFF3.
HPAHPA044512.
MIM601464. gene.
neXtProtNX_P51826.
PharmGKBPA30264.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG69292.
HOVERGENHBG004189.
KOK15195.
OMASVTCEKR.
OrthoDBEOG767390.
PhylomeDBP51826.
TreeFamTF326216.

Gene expression databases

ArrayExpressP51826.
BgeeP51826.
CleanExHS_AFF3.
GenevestigatorP51826.

Family and domain databases

InterProIPR007797. TF_AF4/FMR2.
[Graphical view]
PANTHERPTHR10528. PTHR10528. 1 hit.
PfamPF05110. AF-4. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSAFF3. human.
GeneWikiAFF3.
GenomeRNAi3899.
NextBio15311.
PROP51826.
SOURCESearch...

Entry information

Entry nameAFF3_HUMAN
AccessionPrimary (citable) accession number: P51826
Secondary accession number(s): B7ZM46 expand/collapse secondary AC list , B9EGL9, D3DVI6, Q53RD6, Q53S47, Q53SI6, Q53TB9, Q59F27, Q8IWJ5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: February 20, 2007
Last modified: April 16, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM