SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P51820

- DRTS_SOYBN

UniProt

P51820 - DRTS_SOYBN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Bifunctional dihydrofolate reductase-thymidylate synthase
Gene
N/A
Organism
Glycine max (Soybean) (Glycine hispida)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Bifunctional enzyme. Involved in de novo dTMP biosynthesis. Key enzyme in folate metabolism. Can play two different roles depending on the source of dihydrofolate: de novo synthesis of tetrahydrofolate or recycling of the dihydrofolate released as one of the end products of the TS catalyzed reaction. Catalyzes an essential reaction for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP By similarity.UniRule annotation

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.UniRule annotation
5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei30 – 301Substrate; via carbonyl oxygen By similarity
Binding sitei32 – 321NADP; via amide nitrogen and carbonyl oxygen By similarity
Binding sitei52 – 521Substrate By similarity
Binding sitei139 – 1391Substrate; via carbonyl oxygen By similarity
Binding sitei160 – 1601Substrate By similarity
Binding sitei267 – 2671dUMP By similarity
Active sitei412 – 4121 By similarity
Binding sitei413 – 4131dUMP By similarity
Binding sitei443 – 4431dUMP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi38 – 447NADP By similarity
Nucleotide bindingi76 – 783NADP By similarity
Nucleotide bindingi97 – 1004NADP By similarity
Nucleotide bindingi140 – 1478NADP By similarity
Nucleotide bindingi431 – 4355dUMP By similarity
Nucleotide bindingi473 – 4753dUMP By similarity

GO - Molecular functioni

  1. dihydrofolate reductase activity Source: UniProtKB-EC
  2. thymidylate synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. dTMP biosynthetic process Source: InterPro
  2. glycine biosynthetic process Source: InterPro
  3. one-carbon metabolic process Source: UniProtKB-KW
  4. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Oxidoreductase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis, One-carbon metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-9361.
UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional dihydrofolate reductase-thymidylate synthase
Short name:
DHFR-TS
Including the following 2 domains:
Dihydrofolate reductase (EC:1.5.1.3)
Thymidylate synthase (EC:2.1.1.45)
OrganismiGlycine max (Soybean) (Glycine hispida)
Taxonomic identifieri3847 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja
ProteomesiUP000008827: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 530530Bifunctional dihydrofolate reductase-thymidylate synthaseUniRule annotation
PRO_0000186359Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP51820.

Structurei

3D structure databases

ProteinModelPortaliP51820.
SMRiP51820. Positions 245-530.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 203178DHFR
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni206 – 530325Thymidylate synthaseUniRule annotation
Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the dihydrofolate reductase family.
In the C-terminal section; belongs to the thymidylate synthase family.

Phylogenomic databases

KOiK13998.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
3.40.430.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR024072. DHFR-like_dom.
IPR012262. DHFR-TS.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
PF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF000389. DHFR-TS. 1 hit.
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF53597. SSF53597. 1 hit.
SSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P51820-1 [UniParc]FASTAAdd to Basket

« Hide

MPSDSSVISN GHSNGSVNPL PNLQRTYQVV VAATQDWGIG KDGKLPWRLP    50
TDLKFFKEIT MKTSEPGKKN AIVMGRKTWE SIPLEYRPLS GRLNVVLTRS 100
GSFDIATAEN VVICGSMSSA LELLAASPYS LSIEKVFVIG GGQIFREALN 150
VPGCEAIHLT EIQSSIECDT FMPPVDFTIF RPWYSSFPKV ENNIRYSFTT 200
YVRVRSSAAE SAGQNIDPLL DNNSESMKFE VKDFSFLPKM ISERHEEYLY 250
LKLVQDIIAE GTTKGDRTGT GTLSKFGCQM RFNLRGNFPL LTTKKVFWRG 300
VVEELLWFIS GSTNAKVLQE KGIHIWDGNA SREYLDGVGL TEREEGDLGP 350
VYGFQWRHFG ARYTDMHHDY SGQGFDQLLD VINKIKRNPD DRRIILSAWN 400
PVDLKLMALP PCHMFAQFYV AHGELSCQMY QRSADMGLGI PFNIASYALL 450
TCMIAHVCDL IPGDFIHVIG DAHIYRNHVR PLQEQLHNQP KPFPTLKINP 500
KKKDIDSFVA ADFKLIGYDP HQKIDMKLSV 530
Length:530
Mass (Da):59,745
Last modified:October 1, 1996 - v1
Checksum:iAFD710709FD5D820
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S78087 mRNA. Translation: AAB34317.1.
PIRiS55683.
RefSeqiNP_001238644.1. NM_001251715.1.
UniGeneiGma.483.

Genome annotation databases

GeneIDi547833.
KEGGigmx:547833.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S78087 mRNA. Translation: AAB34317.1 .
PIRi S55683.
RefSeqi NP_001238644.1. NM_001251715.1.
UniGenei Gma.483.

3D structure databases

ProteinModelPortali P51820.
SMRi P51820. Positions 245-530.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 547833.
KEGGi gmx:547833.

Phylogenomic databases

KOi K13998.

Enzyme and pathway databases

UniPathwayi UPA00077 ; UER00158 .
BioCyci MetaCyc:MONOMER-9361.

Gene expression databases

Genevestigatori P51820.

Family and domain databases

Gene3Di 3.30.572.10. 1 hit.
3.40.430.10. 1 hit.
HAMAPi MF_00008. Thymidy_synth_bact.
InterProi IPR024072. DHFR-like_dom.
IPR012262. DHFR-TS.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view ]
Pfami PF00186. DHFR_1. 1 hit.
PF00303. Thymidylat_synt. 1 hit.
[Graphical view ]
PIRSFi PIRSF000389. DHFR-TS. 1 hit.
PRINTSi PR00108. THYMDSNTHASE.
SUPFAMi SSF53597. SSF53597. 1 hit.
SSF55831. SSF55831. 1 hit.
TIGRFAMsi TIGR03284. thym_sym. 1 hit.
PROSITEi PS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning, nucleotide sequence and expression of the bifunctional dihydrofolate reductase-thymidylate synthase from Glycine max."
    Wang M., Ratnam S., Freisheim J.H.
    Biochim. Biophys. Acta 1261:325-336(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.

Entry informationi

Entry nameiDRTS_SOYBN
AccessioniPrimary (citable) accession number: P51820
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi