Reviewed,
UniProtKB/Swiss-Prot P51813 (BMX_HUMAN)
Last modified
November 25, 2008.
Version 87.
History...
Clusters with 100%,
90%,
50% identity |
Documents (7) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Cytoplasmic tyrosine-protein kinase BMX EC=2.7.10.2 Alternative name(s): Bone marrow tyrosine kinase gene in chromosome X protein Epithelial and endothelial tyrosine kinase Short name=ETK NTK38 | ||
| Gene names |
| ||
| Organism | Homo sapiens (Human) | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 675 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Activity is required for interleukin 6 (IL-6) induced differentiation. May play a role in the growth and differentiation of hematopoietic cells. May be involved in signal transduction in endocardial and arterial endothelial cells. |
| Catalytic activity | ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. |
| Cofactor | Binds 1 zinc ion per subunit By similarity. |
| Subunit structure | Interacts with RUFY1 and RUFY2. |
| Subcellular location | CytoplasmProbable. |
| Tissue specificity | Preferentially expressed in epithelial and endothelial cells. |
| Induction | Activated by IL-6 through phosphatidylinositol 3-kinase (PI3-kinase) pathway. It is likely that activation occurs through binding of phosphoinositides to the PH domain. |
| Domain | SH2 domain mediates interaction with RUFY1. |
| Sequence similarities | Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily. Contains 1 Btk-type zinc finger. Contains 1 PH domain. Contains 1 protein kinase domain. Contains 1 SH2 domain. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| CRK | P46108 | 1 | EBI-696657,EBI-886 | |
| PIM1 | P11309 | 2 | EBI-696657,EBI-696621 | |
| TP53 | P04637 | 1 | EBI-696657,EBI-366083 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||||||||||||||||||||
Molecule processing | |||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 675 | 675 | Cytoplasmic tyrosine-protein kinase BMX | PRO_0000088063 | |||||||||||||||||||||||||
Regions | |||||||||||||||||||||||||||||
| Domain | 4 – 111 | 108 | PH | ||||||||||||||||||||||||||
| Domain | 296 – 392 | 97 | SH2 | ||||||||||||||||||||||||||
| Domain | 417 – 675 | 259 | Protein kinase | ||||||||||||||||||||||||||
| Zinc finger | 113 – 149 | 37 | Btk-type | ||||||||||||||||||||||||||
| Nucleotide binding | 423 – 431 | 9 | ATP By similarity | ||||||||||||||||||||||||||
| Compositional bias | 251 – 255 | 5 | Poly-Ser | ||||||||||||||||||||||||||
| Compositional bias | 282 – 285 | 4 | Poly-Ser | ||||||||||||||||||||||||||
| Compositional bias | 286 – 289 | 4 | Poly-Glu | ||||||||||||||||||||||||||
Sites | |||||||||||||||||||||||||||||
| Active site | 536 | 1 | Proton acceptor By similarity | ||||||||||||||||||||||||||
| Metal binding | 121 | 1 | Zinc By similarity | ||||||||||||||||||||||||||
| Metal binding | 132 | 1 | Zinc By similarity | ||||||||||||||||||||||||||
| Metal binding | 133 | 1 | Zinc By similarity | ||||||||||||||||||||||||||
| Metal binding | 143 | 1 | Zinc By similarity | ||||||||||||||||||||||||||
| Binding site | 445 | 1 | ATP By similarity | ||||||||||||||||||||||||||
Amino acid modifications | |||||||||||||||||||||||||||||
| Modified residue | 216 | 1 | Phosphotyrosine; by autocatalysis | ||||||||||||||||||||||||||
| Modified residue | 224 | 1 | Phosphotyrosine; by autocatalysis | ||||||||||||||||||||||||||
| Modified residue | 566 | 1 | Phosphotyrosine; by autocatalysis By similarity | ||||||||||||||||||||||||||
Natural variations | |||||||||||||||||||||||||||||
| Natural variant | 284 | 1 | S → L | VAR_041674 | |||||||||||||||||||||||||
| Natural variant | 670 | 1 | R → W in a lung large cell carcinoma sample; somatic mutation. | VAR_041675 | |||||||||||||||||||||||||
Experimental info | |||||||||||||||||||||||||||||
| Sequence conflict | 207 | 1 | P → G AA sequence Ref.6 | ||||||||||||||||||||||||||
| Sequence conflict | 597 | 1 | A → S in AAA17744. Ref.7 | ||||||||||||||||||||||||||
Secondary structure | |||||||||||||||||||||||||||||
Helix Strand Turn | |||||||||||||||||||||||||||||
| Beta strand | 129 – 133 | 5 | |||||||||||||||||||||||||||
| Beta strand | 136 – 139 | 4 | |||||||||||||||||||||||||||
| Beta strand | 294 – 297 | 4 | |||||||||||||||||||||||||||
| Helix | 303 – 312 | 10 | |||||||||||||||||||||||||||
| Beta strand | 318 – 323 | 6 | |||||||||||||||||||||||||||
| Beta strand | 330 – 334 | 5 | |||||||||||||||||||||||||||
| Beta strand | 347 – 350 | 4 | |||||||||||||||||||||||||||
| Beta strand | 359 – 362 | 4 | |||||||||||||||||||||||||||
| Helix | 370 – 379 | 10 | |||||||||||||||||||||||||||
| Beta strand | 383 – 385 | 3 | |||||||||||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "BMX, a novel nonreceptor tyrosine kinase gene of the BTK/ITK/TEC/TXK family located in chromosome Xp22.2." Tamagnone L., Lahtinen I., Mustonen T., Virtaneva K., Francis F., Muscatelli F., Alitalo R.P., Smith C.I., Larsson C., Alitalo K. Oncogene 9:3683-3688(1994) [PubMed: 7970727] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Bone marrow. |
| [2] | "Etk/Bmx, a tyrosine kinase with a pleckstrin-homology domain, is an effector of phosphatidylinositol 3'-kinase and is involved in interleukin 6-induced neuroendocrine differentiation of prostate cancer cells." Qiu Y., Robinson D., Pretlow T., Kung H.-J. Proc. Natl. Acad. Sci. U.S.A. 95:3644-3649(1998) [PubMed: 9520419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION. Tissue: Prostate. |
| [3] | "The DNA sequence of the human X chromosome." Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. Bentley D.R.Nature 434:325-337(2005) [PubMed: 15772651] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [4] | Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [5] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin. |
| [6] | "Identification of phosphorylation sites within the SH3 domains of Tec family tyrosine kinases." Nore B.F., Mattsson P.T., Antonsson P., Backesjo C.-M., Westlund A., Lennartsson J., Hansson H., Low P., Ronnstrand L., Smith C.I.E. Biochim. Biophys. Acta 1645:123-132(2003) [PubMed: 12573241] [Abstract] Cited for: PROTEIN SEQUENCE OF 207-220 AND 223-236, PHOSPHORYLATION AT TYR-216 AND TYR-224. |
| [7] | Fuortes M. Thesis (1994), Cornell University, United States Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 536-599. Tissue: Blood. |
| [8] | "Interaction between tyrosine kinase Etk and a RUN-domain and FYVE-domain containing protein RUFY1. A possible role of Etk in regulation of vesicle trafficking." Yang J., Kim O., Wu J., Qiu Y. J. Biol. Chem. 277:30219-30226(2002) [PubMed: 11877430] [Abstract] Cited for: INTERACTION WITH RUFY1 AND RUFY2. |
| [9] | "Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. Stratton M.R.Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] LEU-284 AND TRP-670. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| X83107 mRNA. Translation: CAA58169.1. AF045459 mRNA. Translation: AAC08966.1. Different initiation. AC097625 Genomic DNA. No translation available. CH471074 Genomic DNA. Translation: EAW98889.1. BC016652 mRNA. Translation: AAH16652.1. U08341 mRNA. Translation: AAA17744.1. | |||||||||||||||||||
| PIR | S60612. | ||||||||||||||||||
| RefSeq | NP_001712.1. NP_975010.1. | ||||||||||||||||||
| UniGene | Hs.495731 | ||||||||||||||||||
3D structure databases | |||||||||||||||||||
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| ModBase | Search... | ||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||
| IntAct | P51813. | ||||||||||||||||||
PTM databases | |||||||||||||||||||
| PhosphoSite | P51813. | ||||||||||||||||||
Genome annotation databases | |||||||||||||||||||
| Ensembl | ENSG00000102010. Homo sapiens. [Contig view] | ||||||||||||||||||
| GeneID | 660. | ||||||||||||||||||
| KEGG | hsa:660. | ||||||||||||||||||

Clusters with