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Protein

Vesicle-associated membrane protein 7

Gene

VAMP7

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation.3 Publications

Miscellaneous

The gene coding for this protein is located in the pseudoautosomal region 2 (PAR2) of X and Y chromosomes.
Loss-of-function mutant (antisense inhibition) displays impaired granzyme B release and target cell killing by natural killer cells.

GO - Molecular functioni

GO - Biological processi

  • calcium ion regulated exocytosis Source: UniProtKB
  • endocytosis Source: GO_Central
  • endosome to lysosome transport Source: UniProtKB
  • eosinophil degranulation Source: UniProtKB
  • ER to Golgi vesicle-mediated transport Source: UniProtKB
  • Golgi to plasma membrane protein transport Source: Ensembl
  • membrane organization Source: Reactome
  • natural killer cell degranulation Source: UniProtKB
  • neutrophil degranulation Source: UniProtKB
  • phagocytosis, engulfment Source: UniProtKB
  • positive regulation of dendrite morphogenesis Source: Ensembl
  • positive regulation of histamine secretion by mast cell Source: UniProtKB
  • post-Golgi vesicle-mediated transport Source: Reactome
  • regulation of protein targeting to vacuolar membrane Source: Ensembl
  • SNARE complex assembly Source: Ensembl
  • triglyceride transport Source: Ensembl
  • vesicle fusion Source: UniProtKB
  • vesicle fusion with Golgi apparatus Source: Ensembl
  • vesicle-mediated transport Source: UniProtKB
  • vesicle transport along microtubule Source: Ensembl

Keywordsi

Biological processExocytosis, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-421837. Clathrin derived vesicle budding.
R-HSA-432720. Lysosome Vesicle Biogenesis.
R-HSA-432722. Golgi Associated Vesicle Biogenesis.
R-HSA-447115. Interleukin-12 family signaling.
R-HSA-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-HSA-8856828. Clathrin-mediated endocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein 7
Short name:
VAMP-7
Alternative name(s):
Synaptobrevin-like protein 1
Tetanus-insensitive VAMP
Short name:
Ti-VAMP
Gene namesi
Name:VAMP7
Synonyms:SYBL1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

EuPathDBiHostDB:ENSG00000124333.15.
HGNCiHGNC:11486. VAMP7.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 188CytoplasmicSequence analysisAdd BLAST187
Transmembranei189 – 209Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21
Topological domaini210 – 220VesicularSequence analysisAdd BLAST11

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Golgi apparatus, Lysosome, Membrane, Synapse, Synaptosome

Pathology & Biotechi

Organism-specific databases

DisGeNETi6845.
MalaCardsiVAMP7.
OpenTargetsiENSG00000124333.
Orphaneti251510. 46,XY partial gonadal dysgenesis.
PharmGKBiPA162408786.

Polymorphism and mutation databases

BioMutaiVAMP7.
DMDMi1723133.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002067612 – 220Vesicle-associated membrane protein 7Add BLAST219

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine; partial1 Publication1
Modified residuei167PhosphoserineCombined sources1
Modified residuei168PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP51809.
MaxQBiP51809.
PaxDbiP51809.
PeptideAtlasiP51809.
PRIDEiP51809.

PTM databases

iPTMnetiP51809.
PhosphoSitePlusiP51809.
SwissPalmiP51809.

Expressioni

Tissue specificityi

Detected in all tissues tested.

Gene expression databases

BgeeiENSG00000124333.
CleanExiHS_VAMP7.
ExpressionAtlasiP51809. baseline and differential.
GenevisibleiP51809. HS.

Organism-specific databases

HPAiHPA036733.

Interactioni

Subunit structurei

Component of the SNARE complex composed of STX4, SNAP23 and VAMP7 that binds SYT7 during lysosomal exocytosis. Component of the SNARE complex composed of STX7, STX8, VAMP7 and VTI1B that is required for heterotypic fusion of late endosomes with lysosomes. May interact with STX17 (By similarity).By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi112712. 18 interactors.
CORUMiP51809.
IntActiP51809. 38 interactors.
STRINGi9606.ENSP00000262640.

Structurei

Secondary structure

1220
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 10Combined sources6
Beta strandi13 – 19Combined sources7
Beta strandi21 – 23Combined sources3
Helixi26 – 33Combined sources8
Beta strandi38 – 40Combined sources3
Beta strandi42 – 47Combined sources6
Beta strandi50 – 57Combined sources8
Beta strandi60 – 70Combined sources11
Helixi72 – 89Combined sources18
Helixi91 – 95Combined sources5
Helixi103 – 117Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DMWNMR-A1-118[»]
ProteinModelPortaliP51809.
SMRiP51809.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP51809.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 110LonginPROSITE-ProRule annotationAdd BLAST104
Domaini125 – 185v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST61

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0859. Eukaryota.
COG5143. LUCA.
GeneTreeiENSGT00510000047733.
HOGENOMiHOG000042711.
HOVERGENiHBG006675.
InParanoidiP51809.
KOiK08515.
OMAiKLTYTHG.
PhylomeDBiP51809.
TreeFamiTF323448.

Family and domain databases

InterProiView protein in InterPro
IPR011012. Longin-like_dom.
IPR010908. Longin_dom.
IPR001388. Synaptobrevin.
PfamiView protein in Pfam
PF13774. Longin. 1 hit.
PF00957. Synaptobrevin. 1 hit.
PRINTSiPR00219. SYNAPTOBREVN.
SMARTiView protein in SMART
SM01270. Longin. 1 hit.
SUPFAMiSSF64356. SSF64356. 1 hit.
PROSITEiView protein in PROSITE
PS50859. LONGIN. 1 hit.
PS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P51809-1) [UniParc]FASTAAdd to basket
Also known as: Ti-VAMPa/VAMP7a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAILFAVVAR GTTILAKHAW CGGNFLEVTE QILAKIPSEN NKLTYSHGNY
60 70 80 90 100
LFHYICQDRI VYLCITDDDF ERSRAFNFLN EIKKRFQTTY GSRAQTALPY
110 120 130 140 150
AMNSEFSSVL AAQLKHHSEN KGLDKVMETQ AQVDELKGIM VRNIDLVAQR
160 170 180 190 200
GERLELLIDK TENLVDSSVT FKTTSRNLAR AMCMKNLKLT IIIIIVSIVF
210 220
IYIIVSPLCG GFTWPSCVKK
Length:220
Mass (Da):24,935
Last modified:January 23, 2007 - v3
Checksum:i9C1AA5C590375CEF
GO
Isoform 2 (identifier: P51809-2) [UniParc]FASTAAdd to basket
Also known as: Ti-VAMPb/VAMP7b

The sequence of this isoform differs from the canonical sequence as follows:
     145-220: DLVAQRGERL...GFTWPSCVKK → VCHLQNYQQK...IFVLSTSLFL

Show »
Length:260
Mass (Da):30,217
Checksum:iAB6942EF4ACD2978
GO
Isoform 3 (identifier: P51809-3) [UniParc]FASTAAdd to basket
Also known as: Ti-VAMPc/VAMP7c

The sequence of this isoform differs from the canonical sequence as follows:
     28-68: Missing.

Show »
Length:179
Mass (Da):20,121
Checksum:iFE272984659C2B96
GO

Sequence cautioni

The sequence BI547528 differs from that shown. Sequence of unknown origin in the C-terminal part.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti98L → P in BAD96514 (Ref. 5) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01750828 – 68Missing in isoform 3. 1 PublicationAdd BLAST41
Alternative sequenceiVSP_017509145 – 220DLVAQ…SCVKK → VCHLQNYQQKSCSSHVYEEP QAHYYHHHRINCVHLYHCFT SLWWIYMAKLCEEIGKKKLP LTKDMREQGVKSNPCDSSLS HTDRWYLPVSSTLFSLFKIL FHASRFIFVLSTSLFL in isoform 2. 1 PublicationAdd BLAST76

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92396 mRNA. Translation: CAA63133.1.
AJ271736 Genomic DNA. Translation: CAB96816.1.
AJ549301 mRNA. Translation: CAD70593.2.
AJ295938 mRNA. Translation: CAC16891.1.
AK222794 mRNA. Translation: BAD96514.1.
BC056141 mRNA. Translation: AAH56141.1.
BI547528 mRNA. No translation available.
CCDSiCCDS14770.4. [P51809-1]
CCDS48199.1. [P51809-3]
CCDS55548.1. [P51809-2]
RefSeqiNP_001138621.1. NM_001145149.2. [P51809-3]
NP_001172112.1. NM_001185183.1. [P51809-2]
NP_005629.1. NM_005638.5. [P51809-1]
UniGeneiHs.24167.

Genome annotation databases

EnsembliENST00000262640; ENSP00000262640; ENSG00000124333. [P51809-2]
ENST00000286448; ENSP00000286448; ENSG00000124333. [P51809-1]
ENST00000460621; ENSP00000427822; ENSG00000124333. [P51809-3]
GeneIDi6845.
KEGGihsa:6845.
UCSCiuc004fnr.4. human. [P51809-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiVAMP7_HUMAN
AccessioniPrimary (citable) accession number: P51809
Secondary accession number(s): Q53GY7, Q7Z409, Q9H4A7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: September 27, 2017
This is version 174 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families