Reviewed, UniProtKB/Swiss-Prot P51809 (VAMP7_HUMAN)

Names and origin

Protein names

Recommended name:
    Vesicle-associated membrane protein 7
      Short name=VAMP-7
Alternative name(s):
    Synaptobrevin-like protein 1
    Tetanus-insensitive VAMP
      Short name=Ti-VAMP

Gene names

Name:	VAMP7
Synonyms:	SYBL1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	220 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation. Ref.9 Ref.10 Ref.11
Subunit structure	Component of the SNARE complex composed of STX4, SNAP23 and VAMP7 that binds SYT7 during lysosomal exocytosis. Component of the SNARE complex composed of STX7, STX8, VAMP7 and VTI1B that is required for heterotypic fusion of late endosomes with lysosomes in liver cells By similarity.
Subcellular location	Cytoplasmic vesicle › secretory vesicle membrane; Single-pass type IV membrane protein By similarity. Golgi apparatus › trans-Golgi network membrane; Single-pass type IV membrane protein By similarity. Late endosome membrane; Single-pass type IV membrane protein By similarity. Lysosome membrane; Single-pass type IV membrane protein By similarity. Endoplasmic reticulum membrane; Single-pass type IV membrane protein By similarity. Cytoplasmic vesicle › phagosome membrane; Single-pass type IV membrane protein By similarity.
Tissue specificity	Detected in all tissues tested.
Miscellaneous	The gene encoding for this protein is located in the pseudoautosomal region 2 (PAR2) of X and Y chromosomes. Loss-of-function mutant (antisense inhibition) displays impaired granzyme B release and target cell killing by natural killer cells.
Sequence similarities	Belongs to the synaptobrevin family. Contains 1 longin domain. Contains 1 v-SNARE coiled-coil homology domain.

Ontologies

Keywords
Biological process	Exocytosis Protein transport Transport
Cellular component	Cytoplasmic vesicle Endoplasmic reticulum Endosome Golgi apparatus Lysosome Membrane
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Signal-anchor Transmembrane
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	ER to Golgi vesicle-mediated transport Inferred from sequence or structural similarity. Source: UniProtKB calcium ion-dependent exocytosis Inferred from sequence or structural similarity. Source: UniProtKB endosome to lysosome transport Ref.9 Inferred from direct assay. Source: UniProtKB eosinophil degranulation Inferred from sequence or structural similarity. Source: UniProtKB neutrophil degranulation Inferred from sequence or structural similarity. Source: UniProtKB phagocytosis, engulfment Inferred from sequence or structural similarity. Source: UniProtKB post-Golgi vesicle-mediated transport Inferred from Experiment. Source: Reactome protein transport Inferred from electronic annotation. Source: UniProtKB-KW vesicle fusion Ref.9 Inferred from direct assay. Source: UniProtKB
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW SNARE complex Inferred from sequence or structural similarity. Source: UniProtKB endoplasmic reticulum membrane Inferred from sequence or structural similarity. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell late endosome membrane Inferred from sequence or structural similarity. Source: UniProtKB lysosomal membrane Inferred from sequence or structural similarity. Source: UniProtKB phagocytic vesicle Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	protein binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
AGFG1	P52594	5	EBI-1052205,EBI-996560

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P51809-1)

Also known as: Ti-VAMPa/VAMP7a;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P51809-2)

Also known as: Ti-VAMPb/VAMP7b;

The sequence of this isoform differs from the canonical sequence as follows:
     145-220: DLVAQRGERL...GFTWPSCVKK → VCHLQNYQQK...IFVLSTSLFL

Isoform 3 (identifier: P51809-3)

Also known as: Ti-VAMPc/VAMP7c;

The sequence of this isoform differs from the canonical sequence as follows:
     28-68: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.8
	Chain	2 – 220	219	Vesicle-associated membrane protein 7		PRO_0000206761
Regions
	Topological domain	2 – 188	187	Cytoplasmic Potential
	Transmembrane	189 – 209	21	Anchor for type IV membrane protein Potential
	Topological domain	210 – 220	11	Vesicular Potential
	Domain	7 – 110	104	Longin
	Domain	125 – 185	61	v-SNARE coiled-coil homology
Amino acid modifications
	Modified residue	2	1	N-acetylalanine; partial Ref.8
	Modified residue	168	1	Phosphoserine Ref.13
Natural variations
	Alternative sequence	28 – 68	41	Missing in isoform 3.		VSP_017508
	Alternative sequence	145 – 220	76	DLVAQ…SCVKK → VCHLQNYQQKSCSSHVYEEP QAHYYHHHRINCVHLYHCFT SLWWIYMAKLCEEIGKKKLP LTKDMREQGVKSNPCDSSLS HTDRWYLPVSSTLFSLFKIL FHASRFIFVLSTSLFL in isoform 2.		VSP_017509
Experimental info
	Sequence conflict	98	1	L → P in BAD96514. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Ti-VAMPa/VAMP7a) [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 9C1AA5C590375CEF	FASTA	220	24,935
	10 20 30 40 50 60 MAILFAVVAR GTTILAKHAW CGGNFLEVTE QILAKIPSEN NKLTYSHGNY LFHYICQDRI 70 80 90 100 110 120 VYLCITDDDF ERSRAFNFLN EIKKRFQTTY GSRAQTALPY AMNSEFSSVL AAQLKHHSEN 130 140 150 160 170 180 KGLDKVMETQ AQVDELKGIM VRNIDLVAQR GERLELLIDK TENLVDSSVT FKTTSRNLAR 190 200 210 220 AMCMKNLKLT IIIIIVSIVF IYIIVSPLCG GFTWPSCVKK
	Isoform 2 (Ti-VAMPb/VAMP7b). Checksum: AB6942EF4ACD2978	FASTA	260	30,217
	10 20 30 40 50 60 MAILFAVVAR GTTILAKHAW CGGNFLEVTE QILAKIPSEN NKLTYSHGNY LFHYICQDRI 70 80 90 100 110 120 VYLCITDDDF ERSRAFNFLN EIKKRFQTTY GSRAQTALPY AMNSEFSSVL AAQLKHHSEN 130 140 150 160 170 180 KGLDKVMETQ AQVDELKGIM VRNIVCHLQN YQQKSCSSHV YEEPQAHYYH HHRINCVHLY 190 200 210 220 230 240 HCFTSLWWIY MAKLCEEIGK KKLPLTKDMR EQGVKSNPCD SSLSHTDRWY LPVSSTLFSL 250 260 FKILFHASRF IFVLSTSLFL
	Isoform 3 (Ti-VAMPc/VAMP7c). Checksum: FE272984659C2B96	FASTA	179	20,121
	10 20 30 40 50 60 MAILFAVVAR GTTILAKHAW CGGNFLEDFE RSRAFNFLNE IKKRFQTTYG SRAQTALPYA 70 80 90 100 110 120 MNSEFSSVLA AQLKHHSENK GLDKVMETQA QVDELKGIMV RNIDLVAQRG ERLELLIDKT 130 140 150 160 170 ENLVDSSVTF KTTSRNLARA MCMKNLKLTI IIIIVSIVFI YIIVSPLCGG FTWPSCVKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A synaptobrevin-like gene in the Xq28 pseudoautosomal region undergoes X inactivation." D'Esposito M., Ciccodicola A., Gianfrancesco F., Esposito T., Flagiello L., Mazzarella R., Schlessinger D., D'Urso M. Nat. Genet. 13:227-229(1996) [PubMed: 8640232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Differentially regulated and evolved genes in the fully sequenced Xq/Yq pseudoautosomal region." Ciccodicola A., D'Esposito M., Esposito T., Gianfrancesco F., Migliaccio C., Miano M.G., Matarazzo M.R., Vacca M., Franze A., Cuccurese M., Cocchia M., Curci A., Terracciano A., Torino A., Cocchia S., Mercadante G., Pannone E., Archidiacono N. , Rocchi M., Schlessinger D., D'Urso M. Hum. Mol. Genet. 9:395-401(2000) [PubMed: 10655549] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"A dual mechansim controlling the localization and function of exocytic v-SNARE." Martinez-Arca S., Rudge R., Vacca M., Camonis J., Daviet L., Formstecher E., Hamburger A., Filippini F., D'Esposito M., Galli T. Proc. Natl. Acad. Sci. U.S.A. 100:9011-9016(2003) [PubMed: 12853575] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3). Tissue: Brain.
[4]	"Alternative splicing of SYBL1 gene." D'Esposito M., Filippini F., Rossi V., D'Urso M. Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[5]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Liver.
[6]	"The DNA sequence of the human X chromosome." Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R. Nature 434:325-337(2005) [PubMed: 15772651] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Uterus.
[8]	Bienvenut W.V. Submitted (JUN-2005) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-10; 126-137 AND 143-150, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, MASS SPECTROMETRY. Tissue: B-cell lymphoma.
[9]	"Syntaxin 7 and VAMP-7 are soluble N-ethylmaleimide-sensitive factor attachment protein receptors required for late endosome-lysosome and homotypic lysosome fusion in alveolar macrophages." Ward D.M., Pevsner J., Scullion M.A., Vaughn M., Kaplan J. Mol. Biol. Cell 11:2327-2333(2000) [PubMed: 10888671] [Abstract] Cited for: FUNCTION.
[10]	"A critical role for vesicle-associated membrane protein-7 in exocytosis from human eosinophils and neutrophils." Logan M.R., Lacy P., Odemuyiwa S.O., Steward M., Davoine F., Kita H., Moqbel R. Allergy 61:777-784(2006) [PubMed: 16677249] [Abstract] Cited for: FUNCTION.
[11]	"Vesicle-associated membrane protein 7 (VAMP-7) is essential for target cell killing in a natural killer cell line." Marcet-Palacios M., Odemuyiwa S.O., Coughlin J.J., Garofoli D., Ewen C., Davidson C.E., Ghaffari M., Kane K.P., Lacy P., Logan M.R., Befus A.D., Bleackley R.C., Moqbel R. Biochem. Biophys. Res. Commun. 366:617-623(2008) [PubMed: 18042464] [Abstract] Cited for: FUNCTION.
[12]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[13]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-168, MASS SPECTROMETRY.
[14]	"Solution structure of the longin domain of synaptobrevin-like protein 1." RIKEN structural genomics initiative (RSGI) Submitted (OCT-2006) to the PDB data bank Cited for: STRUCTURE BY NMR OF 1-118.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X92396 mRNA. Translation: CAA63133.1.
AJ271736 Genomic DNA. Translation: CAB96816.1.
AJ549301 mRNA. Translation: CAD70593.2.
AJ295938 mRNA. Translation: CAC16891.1.
AK222794 mRNA. Translation: BAD96514.1.
BC056141 mRNA. Translation: AAH56141.1.

IPI

IPI00013236.
IPI00020887.
IPI00401804.

RefSeq

NP_001138621.1.
NP_005629.1.

UniGene

Hs.24167

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2DMW	NMR	-	A	1-118	[»]
2VX8	X-ray	2.20	A/B/C/D	1-120	[»]

SMR

P51809. Positions 1-118, 124-186.

ModBase

Search...

Protein-protein interaction databases

IntAct

P51809. 3 interactions.

STRING

P51809.

PTM databases

PhosphoSite

P51809.

Proteomic databases

PRIDE

P51809.

Genome annotation databases

Ensembl

ENST00000286448; ENSP00000286448; ENSG00000124333; Homo sapiens. [Genome view]

GeneID

6845.

KEGG

hsa:6845.

UCSC

uc004fnr.1. human.
uc004fns.1. human.
uc004fnt.1. human.

Organism-specific databases

CTD

6845.

GeneCards

GC0XP154764.

H-InvDB

HIX0028460.
HIX0077340.

HGNC

HGNC:11486. VAMP7.

MIM

300053. gene.

PharmGKB

PA36268.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG09091.

HOVERGEN

P51809.

OMA

QLKYHSE.

OrthoDB

EOG9X3KM4.

PhylomeDB

P51809.

Enzyme and pathway databases

Reactome

REACT_11123. Membrane Trafficking.

Gene expression databases

ArrayExpress

P51809.

Bgee

P51809.

CleanEx

HS_VAMP7.

Genevestigator

P51809.

GermOnline

ENSG00000124333. Homo sapiens.

Family and domain databases

InterPro

IPR010908. Longin.
IPR011012. Longin-like.
IPR001388. Synaptobrevin.
[Graphical view]

Pfam

PF00957. Synaptobrevin. 1 hit.
[Graphical view]

PRINTS

PR00219. SYNAPTOBREVN.

PROSITE

PS50859. LONGIN. 1 hit.
PS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

26723.

SOURCE

Search...

Entry information

Entry name

VAMP7_HUMAN

Accession

Primary (citable) accession number: P51809
Secondary accession number(s): Q53GY7, Q7Z409, Q9H4A7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 23, 2007
Last modified:	January 19, 2010
This is version 95 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.