Reviewed,
UniProtKB/Swiss-Prot P51809 (VAMP7_HUMAN)
Last modified
January 19, 2010.
Version 95.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
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Names and origin
| Protein names | Recommended name: Vesicle-associated membrane protein 7 Short name=VAMP-7 Alternative name(s): Synaptobrevin-like protein 1 Tetanus-insensitive VAMP Short name=Ti-VAMP | ||||
| Gene names |
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| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 220 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation. Ref.9 Ref.10 Ref.11 |
| Subunit structure | Component of the SNARE complex composed of STX4, SNAP23 and VAMP7 that binds SYT7 during lysosomal exocytosis. Component of the SNARE complex composed of STX7, STX8, VAMP7 and VTI1B that is required for heterotypic fusion of late endosomes with lysosomes in liver cells By similarity. |
| Subcellular location | Cytoplasmic vesicle › secretory vesicle membrane; Single-pass type IV membrane protein By similarity. Golgi apparatus › trans-Golgi network membrane; Single-pass type IV membrane protein By similarity. Late endosome membrane; Single-pass type IV membrane protein By similarity. Lysosome membrane; Single-pass type IV membrane protein By similarity. Endoplasmic reticulum membrane; Single-pass type IV membrane protein By similarity. Cytoplasmic vesicle › phagosome membrane; Single-pass type IV membrane protein By similarity. |
| Tissue specificity | Detected in all tissues tested. |
| Miscellaneous | The gene encoding for this protein is located in the pseudoautosomal region 2 (PAR2) of X and Y chromosomes. Loss-of-function mutant (antisense inhibition) displays impaired granzyme B release and target cell killing by natural killer cells. |
| Sequence similarities | Belongs to the synaptobrevin family. Contains 1 longin domain. Contains 1 v-SNARE coiled-coil homology domain. |
Ontologies
Alternative products
| This entry describes 3 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: P51809-1) Also known as: Ti-VAMPa/VAMP7a; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: P51809-2) Also known as: Ti-VAMPb/VAMP7b; The sequence of this isoform differs from the canonical sequence as follows: 145-220: DLVAQRGERL...GFTWPSCVKK → VCHLQNYQQK...IFVLSTSLFL | ||||||
| Isoform 3 (identifier: P51809-3) Also known as: Ti-VAMPc/VAMP7c; The sequence of this isoform differs from the canonical sequence as follows: 28-68: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed Ref.8 | ||||||
| Chain | 2 – 220 | 219 | Vesicle-associated membrane protein 7 | PRO_0000206761 | |||||
Regions | |||||||||
| Topological domain | 2 – 188 | 187 | Cytoplasmic Potential | ||||||
| Transmembrane | 189 – 209 | 21 | Anchor for type IV membrane protein Potential | ||||||
| Topological domain | 210 – 220 | 11 | Vesicular Potential | ||||||
| Domain | 7 – 110 | 104 | Longin | ||||||
| Domain | 125 – 185 | 61 | v-SNARE coiled-coil homology | ||||||
Amino acid modifications | |||||||||
| Modified residue | 2 | 1 | N-acetylalanine; partial Ref.8 | ||||||
| Modified residue | 168 | 1 | Phosphoserine Ref.13 | ||||||
Natural variations | |||||||||
| Alternative sequence | 28 – 68 | 41 | Missing in isoform 3. | VSP_017508 | |||||
| Alternative sequence | 145 – 220 | 76 | DLVAQ…SCVKK → VCHLQNYQQKSCSSHVYEEP QAHYYHHHRINCVHLYHCFT SLWWIYMAKLCEEIGKKKLP LTKDMREQGVKSNPCDSSLS HTDRWYLPVSSTLFSLFKIL FHASRFIFVLSTSLFL in isoform 2. | VSP_017509 | |||||
Experimental info | |||||||||
| Sequence conflict | 98 | 1 | L → P in BAD96514. Ref.5 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "A synaptobrevin-like gene in the Xq28 pseudoautosomal region undergoes X inactivation." D'Esposito M., Ciccodicola A., Gianfrancesco F., Esposito T., Flagiello L., Mazzarella R., Schlessinger D., D'Urso M. Nat. Genet. 13:227-229(1996) [PubMed: 8640232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). |
| [2] | "Differentially regulated and evolved genes in the fully sequenced Xq/Yq pseudoautosomal region." Ciccodicola A., D'Esposito M., Esposito T., Gianfrancesco F., Migliaccio C., Miano M.G., Matarazzo M.R., Vacca M., Franze A., Cuccurese M., Cocchia M., Curci A., Terracciano A., Torino A., Cocchia S., Mercadante G., Pannone E., Archidiacono N. D'Urso M.Hum. Mol. Genet. 9:395-401(2000) [PubMed: 10655549] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | "A dual mechansim controlling the localization and function of exocytic v-SNARE." Martinez-Arca S., Rudge R., Vacca M., Camonis J., Daviet L., Formstecher E., Hamburger A., Filippini F., D'Esposito M., Galli T. Proc. Natl. Acad. Sci. U.S.A. 100:9011-9016(2003) [PubMed: 12853575] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3). Tissue: Brain. |
| [4] | "Alternative splicing of SYBL1 gene." D'Esposito M., Filippini F., Rossi V., D'Urso M. Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). |
| [5] | Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Liver. |
| [6] | "The DNA sequence of the human X chromosome." Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. Bentley D.R.Nature 434:325-337(2005) [PubMed: 15772651] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [7] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Uterus. |
| [8] | Bienvenut W.V. Submitted (JUN-2005) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-10; 126-137 AND 143-150, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, MASS SPECTROMETRY. Tissue: B-cell lymphoma. |
| [9] | "Syntaxin 7 and VAMP-7 are soluble N-ethylmaleimide-sensitive factor attachment protein receptors required for late endosome-lysosome and homotypic lysosome fusion in alveolar macrophages." Ward D.M., Pevsner J., Scullion M.A., Vaughn M., Kaplan J. Mol. Biol. Cell 11:2327-2333(2000) [PubMed: 10888671] [Abstract] Cited for: FUNCTION. |
| [10] | "A critical role for vesicle-associated membrane protein-7 in exocytosis from human eosinophils and neutrophils." Logan M.R., Lacy P., Odemuyiwa S.O., Steward M., Davoine F., Kita H., Moqbel R. Allergy 61:777-784(2006) [PubMed: 16677249] [Abstract] Cited for: FUNCTION. |
| [11] | "Vesicle-associated membrane protein 7 (VAMP-7) is essential for target cell killing in a natural killer cell line." Marcet-Palacios M., Odemuyiwa S.O., Coughlin J.J., Garofoli D., Ewen C., Davidson C.E., Ghaffari M., Kane K.P., Lacy P., Logan M.R., Befus A.D., Bleackley R.C., Moqbel R. Biochem. Biophys. Res. Commun. 366:617-623(2008) [PubMed: 18042464] [Abstract] Cited for: FUNCTION. |
| [12] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| [13] | "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-168, MASS SPECTROMETRY. |
| [14] | "Solution structure of the longin domain of synaptobrevin-like protein 1." RIKEN structural genomics initiative (RSGI) Submitted (OCT-2006) to the PDB data bank Cited for: STRUCTURE BY NMR OF 1-118. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EMBL GenBank DDBJ | X92396 mRNA. Translation: CAA63133.1. AJ271736 Genomic DNA. Translation: CAB96816.1. AJ549301 mRNA. Translation: CAD70593.2. AJ295938 mRNA. Translation: CAC16891.1. AK222794 mRNA. Translation: BAD96514.1. BC056141 mRNA. Translation: AAH56141.1. | ||||||||||||||||||
| IPI | IPI00013236. IPI00020887. IPI00401804. | ||||||||||||||||||
| RefSeq | NP_001138621.1. NP_005629.1. | ||||||||||||||||||
| UniGene | Hs.24167 | ||||||||||||||||||
3D structure databases | |||||||||||||||||||
| PDBe RCSB PDB PDBj |
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| SMR | P51809. Positions 1-118, 124-186. | ||||||||||||||||||
| ModBase | Search... | ||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||
| IntAct | P51809. 3 interactions. | ||||||||||||||||||
| STRING | P51809. | ||||||||||||||||||
PTM databases | |||||||||||||||||||
| PhosphoSite | P51809. | ||||||||||||||||||
Proteomic databases | |||||||||||||||||||
| PRIDE | P51809. | ||||||||||||||||||
Genome annotation databases | |||||||||||||||||||
| Ensembl | ENST00000286448; ENSP00000286448; ENSG00000124333; Homo sapiens. [Genome view] | ||||||||||||||||||
| GeneID | 6845. | ||||||||||||||||||
| KEGG | hsa:6845. | ||||||||||||||||||
| UCSC | uc004fnr.1. human. uc004fns.1. human. uc004fnt.1. human. | ||||||||||||||||||
Organism-specific databases | |||||||||||||||||||
| CTD | 6845. | ||||||||||||||||||
| GeneCards | GC0XP154764. | ||||||||||||||||||
| H-InvDB | HIX0028460. HIX0077340. | ||||||||||||||||||
| HGNC | HGNC:11486. VAMP7. | ||||||||||||||||||
| MIM | 300053. gene. | ||||||||||||||||||
| PharmGKB | PA36268. | ||||||||||||||||||
| GenAtlas | Search... | ||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||
| eggNOG | prNOG09091. | ||||||||||||||||||
| HOVERGEN | P51809. | ||||||||||||||||||
| OMA | QLKYHSE. | ||||||||||||||||||
| OrthoDB | EOG9X3KM4. | ||||||||||||||||||
| PhylomeDB | P51809. | ||||||||||||||||||
Enzyme and pathway databases | |||||||||||||||||||
| Reactome | REACT_11123. Membrane Trafficking. | ||||||||||||||||||
Gene expression databases | |||||||||||||||||||
| ArrayExpress | P51809. | ||||||||||||||||||
| Bgee | P51809. | ||||||||||||||||||
| CleanEx | HS_VAMP7. | ||||||||||||||||||
| Genevestigator | P51809. | ||||||||||||||||||
| GermOnline | ENSG00000124333. Homo sapiens. | ||||||||||||||||||
Family and domain databases | |||||||||||||||||||
| InterPro | IPR010908. Longin. IPR011012. Longin-like. IPR001388. Synaptobrevin. [Graphical view] | ||||||||||||||||||
| Pfam | PF00957. Synaptobrevin. 1 hit. [Graphical view] | ||||||||||||||||||
| PRINTS | PR00219. SYNAPTOBREVN. | ||||||||||||||||||
| PROSITE | PS50859. LONGIN. 1 hit. PS00417. SYNAPTOBREVIN. 1 hit. PS50892. V_SNARE. 1 hit. [Graphical view] | ||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||
Other Resources | |||||||||||||||||||
| NextBio | 26723. | ||||||||||||||||||
| SOURCE | Search... | ||||||||||||||||||
Entry information
| Entry name | VAMP7_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P51809 Secondary accession number(s): Q53GY7, Q7Z409, Q9H4A7 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome X Human chromosome X: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


