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P51807

- DYLT1_MOUSE

UniProt

P51807 - DYLT1_MOUSE

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Protein

Dynein light chain Tctex-type 1

Gene

Dynlt1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Binds to transport cargos and is involved in apical cargo transport such as rhodopsin-bearing vesicles in polarized epithelia. Is involved in intracellular targeting of D-type retrovirus gag polyproteins to the cytoplasmic assembly site (By similarity). May also be a accessory component of axonemal dynein. Plays an important role in male germ cell development and function. Candidate for involvement in male sterility.By similarity
Plays a role in neuronal morphogenesis; the function is independent of cytoplasmic dynein and seems to be coupled to regulation of the actin cytoskeleton by enhancing Rac1 activity. The function in neurogenesis may be regulated by association with a G-protein beta-gamma dimer. May function as a receptor-independent activator of heterotrimeric G-protein signaling; the activation appears to be independent of a nucleotide exchange. Plays a role in regulating neurogenesis; inhibits the genesis of neurons from precursor cells during cortical development presumably by antagonizing ARHGEF2. Involved in the regulation of mitotic spindle orientation.

GO - Molecular functioni

  1. motor activity Source: UniProtKB-KW

GO - Biological processi

  1. establishment of mitotic spindle orientation Source: UniProtKB
  2. mitotic nuclear division Source: UniProtKB-KW
  3. negative regulation of neurogenesis Source: UniProtKB
  4. regulation of G-protein coupled receptor protein signaling pathway Source: UniProtKB
  5. transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Neurogenesis, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Dynein light chain Tctex-type 1
Alternative name(s):
Activator of G-protein signaling 2
Short name:
AGS2
T-complex testis-specific protein 1
TCTEX-1
Gene namesi
Name:Dynlt1
Synonyms:Tctel1, Tctex-1, Tctex1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:98643. Dynlt1.

Subcellular locationi

Golgi apparatus By similarity. Cytoplasm By similarity. Cytoplasmcytoskeletonspindle By similarity
Note: Localizes to mitotic spindles.By similarity

GO - Cellular componenti

  1. cytoplasmic dynein complex Source: UniProtKB
  2. Golgi apparatus Source: UniProtKB-KW
  3. microtubule Source: UniProtKB-KW
  4. photoreceptor inner segment Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Dynein, Golgi apparatus, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 113113Dynein light chain Tctex-type 1PRO_0000195153Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Post-translational modificationi

Phosphorylated by BMPR2. The phosphorylation status is proposed to regulate the association with the cytoplasmic dynein complex and may have role in cytoplasmic dynein cargo release (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP51807.
PaxDbiP51807.
PRIDEiP51807.

PTM databases

PhosphoSiteiP51807.

Expressioni

Tissue specificityi

High level in testis (germ cell-specific). Expressed in sperm (at protein level). 200-fold lower in liver, brain, heart, spleen, and kidney. Levels in thymus and two embryonal carcinoma cell lines were several-fold higher than this low constitutive level.1 Publication

Developmental stagei

First abundantly expressed at the pachytene stage of meiosis and persists throughout spermatogenesis.

Gene expression databases

BgeeiP51807.
CleanExiMM_DYNLT1.
GenevestigatoriP51807.

Interactioni

Subunit structurei

Homodimer (Probable). The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs); the composition seems to vary in respect to the IC, LIC and LC composition. The heavy chain homodimer serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. The ICs and LICs bind directly to the HC dimer and the LCs assemble on the IC dimer. DYNLT1 and DYNLT3 compete for association with dynein IC (DYNC1I1 or DYNC1I2). Self-associates. Interacts with RHO (By similarity). Interacts with DYNC1I1 and DYNC1I2. Interacts with DOC2A, DOC2B and SCN10A. Interacts with PVR. Interacts with SVIL isoform 2. Interacts with GNB1; the interaction occurs in presence of guanine nucleotide-binding protein G(T) subunit gamma; the interaction diminishes the association of DYNLT1 with dynein IC (DYNC1I1 or DYNC1I2). Interacts with GNB2, GNB3 and GNB5; the interactions occur in presence of guanine nucleotide-binding protein G(T) subunit gamma (By similarity).By similarityCurated

Binary interactionsi

WithEntry#Exp.IntActNotes
FynP396883EBI-642797,EBI-524514
SVILO463854EBI-642797,EBI-6995105From a different organism.

Protein-protein interaction databases

BioGridi204082. 2 interactions.
IntActiP51807. 9 interactions.
MINTiMINT-7901686.

Structurei

3D structure databases

ProteinModelPortaliP51807.
SMRiP51807. Positions 12-113.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni41 – 11373Interaction with GNB1By similarityAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG278055.
GeneTreeiENSGT00390000013998.
HOGENOMiHOG000255148.
HOVERGENiHBG001217.
InParanoidiP51807.
KOiK10420.
OMAiSCFWDNS.
OrthoDBiEOG7PK918.
PhylomeDBiP51807.
TreeFamiTF313904.

Family and domain databases

InterProiIPR005334. Tctex.
[Graphical view]
PANTHERiPTHR21255. PTHR21255. 1 hit.
PfamiPF03645. Tctex-1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P51807-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEDFQASEET AFVVDEVSSI VKEAIESAIG GNAYQHSKVN QWTTNVLEQT
60 70 80 90 100
LSQLTKLGRP FKYIVTCVIM QKNGAGLHSA SSCFWDSSTD GSCTVRWENK
110
TMYCIVSTFG LSI
Length:113
Mass (Da):12,483
Last modified:October 1, 1996 - v1
Checksum:i92DF96F933DB7AF1
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti21 – 211V → A.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M25825 mRNA. Translation: AAA40408.1.
AK133788 mRNA. Translation: BAE21842.1.
AK153971 mRNA. Translation: BAE32288.1.
AK168478 mRNA. Translation: BAE40367.1.
BC043018 mRNA. Translation: AAH43018.1.
BC087868 mRNA. Translation: AAH87868.1.
CCDSiCCDS49936.1.
PIRiA32995.
RefSeqiNP_001160099.1. NM_001166627.1.
NP_001160101.1. NM_001166629.2.
NP_001160102.1. NM_001166630.1.
NP_033368.1. NM_009342.2.
UniGeneiMm.1948.
Mm.381893.
Mm.428775.
Mm.457381.
Mm.457987.
Mm.458102.
Mm.458208.
Mm.463722.

Genome annotation databases

EnsembliENSMUST00000092966; ENSMUSP00000090644; ENSMUSG00000000579.
ENSMUST00000169415; ENSMUSP00000127990; ENSMUSG00000092074.
ENSMUST00000179554; ENSMUSP00000135978; ENSMUSG00000095677.
ENSMUST00000179569; ENSMUSP00000137171; ENSMUSG00000096255.
GeneIDi100040531.
100040563.
100310872.
21648.
KEGGimmu:100040531.
mmu:100040563.
mmu:100310872.
mmu:21648.
UCSCiuc008agl.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M25825 mRNA. Translation: AAA40408.1 .
AK133788 mRNA. Translation: BAE21842.1 .
AK153971 mRNA. Translation: BAE32288.1 .
AK168478 mRNA. Translation: BAE40367.1 .
BC043018 mRNA. Translation: AAH43018.1 .
BC087868 mRNA. Translation: AAH87868.1 .
CCDSi CCDS49936.1.
PIRi A32995.
RefSeqi NP_001160099.1. NM_001166627.1.
NP_001160101.1. NM_001166629.2.
NP_001160102.1. NM_001166630.1.
NP_033368.1. NM_009342.2.
UniGenei Mm.1948.
Mm.381893.
Mm.428775.
Mm.457381.
Mm.457987.
Mm.458102.
Mm.458208.
Mm.463722.

3D structure databases

ProteinModelPortali P51807.
SMRi P51807. Positions 12-113.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 204082. 2 interactions.
IntActi P51807. 9 interactions.
MINTi MINT-7901686.

PTM databases

PhosphoSitei P51807.

Proteomic databases

MaxQBi P51807.
PaxDbi P51807.
PRIDEi P51807.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000092966 ; ENSMUSP00000090644 ; ENSMUSG00000000579 .
ENSMUST00000169415 ; ENSMUSP00000127990 ; ENSMUSG00000092074 .
ENSMUST00000179554 ; ENSMUSP00000135978 ; ENSMUSG00000095677 .
ENSMUST00000179569 ; ENSMUSP00000137171 ; ENSMUSG00000096255 .
GeneIDi 100040531.
100040563.
100310872.
21648.
KEGGi mmu:100040531.
mmu:100040563.
mmu:100310872.
mmu:21648.
UCSCi uc008agl.2. mouse.

Organism-specific databases

CTDi 100040531.
100040563.
100310872.
21648.
MGIi MGI:98643. Dynlt1.

Phylogenomic databases

eggNOGi NOG278055.
GeneTreei ENSGT00390000013998.
HOGENOMi HOG000255148.
HOVERGENi HBG001217.
InParanoidi P51807.
KOi K10420.
OMAi SCFWDNS.
OrthoDBi EOG7PK918.
PhylomeDBi P51807.
TreeFami TF313904.

Miscellaneous databases

NextBioi 300916.
PROi P51807.
SOURCEi Search...

Gene expression databases

Bgeei P51807.
CleanExi MM_DYNLT1.
Genevestigatori P51807.

Family and domain databases

InterProi IPR005334. Tctex.
[Graphical view ]
PANTHERi PTHR21255. PTHR21255. 1 hit.
Pfami PF03645. Tctex-1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "tctex-1: a candidate gene family for a mouse t complex sterility locus."
    Lader E., Ha H.-S., O'Neill M., Artzt K., Bennett D.
    Cell 58:969-979(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Testis.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Liver and Thymus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain and Testis.
  4. "Identification of the t complex-encoded cytoplasmic dynein light chain tctex1 in inner arm I1 supports the involvement of flagellar dyneins in meiotic drive."
    Harrison A., Olds-Clarke P., King S.M.
    J. Cell Biol. 140:1137-1147(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, POSSIBLE FUNCTION IN AXONEMAL DYNEIN.
  5. "Receptor-independent activators of heterotrimeric G-protein signaling pathways."
    Takesono A., Cismowski M.J., Ribas C., Bernard M., Chung P., Hazard S. III, Duzic E., Lanier S.M.
    J. Biol. Chem. 274:33202-33205(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN RECEPTOR-INDEPENDENT ACTIVATION OF G-PROTEIN SIGNALING, ASSOCIATION WITH GBETA-GAMMA.
  6. "Structure of Tctex-1 and its interaction with cytoplasmic dynein intermediate chain."
    Mok Y.K., Lo K.W., Zhang M.
    J. Biol. Chem. 276:14067-14074(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DYNC1I1, SUBUNIT.
  7. "The Tctex1/Tctex2 class of dynein light chains. Dimerization, differential expression, and interaction with the LC8 protein family."
    DiBella L.M., Benashski S.E., Tedford H.W., Harrison A., Patel-King R.S., King S.M.
    J. Biol. Chem. 276:14366-14373(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SELF-ASSOCIATION.
  8. "Lfc and Tctex-1 regulate the genesis of neurons from cortical precursor cells."
    Gauthier-Fisher A., Lin D.C., Greeve M., Kaplan D.R., Rottapel R., Miller F.D.
    Nat. Neurosci. 12:735-744(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN NEUROGENESIS.

Entry informationi

Entry nameiDYLT1_MOUSE
AccessioniPrimary (citable) accession number: P51807
Secondary accession number(s): Q5M8S6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3