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P51807

- DYLT1_MOUSE

UniProt

P51807 - DYLT1_MOUSE

Protein

Dynein light chain Tctex-type 1

Gene

Dynlt1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Binds to transport cargos and is involved in apical cargo transport such as rhodopsin-bearing vesicles in polarized epithelia. Is involved in intracellular targeting of D-type retrovirus gag polyproteins to the cytoplasmic assembly site By similarity. May also be a accessory component of axonemal dynein. Plays an important role in male germ cell development and function. Candidate for involvement in male sterility.By similarity
    Plays a role in neuronal morphogenesis; the function is independent of cytoplasmic dynein and seems to be coupled to regulation of the actin cytoskeleton by enhancing Rac1 activity. The function in neurogenesis may be regulated by association with a G-protein beta-gamma dimer. May function as a receptor-independent activator of heterotrimeric G-protein signaling; the activation appears to be independent of a nucleotide exchange. Plays a role in regulating neurogenesis; inhibits the genesis of neurons from precursor cells during cortical development presumably by antagonizing ARHGEF2. Involved in the regulation of mitotic spindle orientation.

    GO - Molecular functioni

    1. motor activity Source: UniProtKB-KW
    2. protein binding Source: IntAct

    GO - Biological processi

    1. establishment of mitotic spindle orientation Source: UniProtKB
    2. mitotic nuclear division Source: UniProtKB-KW
    3. negative regulation of neurogenesis Source: UniProtKB
    4. regulation of G-protein coupled receptor protein signaling pathway Source: UniProtKB
    5. transport Source: UniProtKB-KW

    Keywords - Molecular functioni

    Motor protein

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis, Neurogenesis, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dynein light chain Tctex-type 1
    Alternative name(s):
    Activator of G-protein signaling 2
    Short name:
    AGS2
    T-complex testis-specific protein 1
    TCTEX-1
    Gene namesi
    Name:Dynlt1
    Synonyms:Tctel1, Tctex-1, Tctex1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:98643. Dynlt1.

    Subcellular locationi

    Golgi apparatus By similarity. Cytoplasm By similarity. Cytoplasmcytoskeletonspindle By similarity
    Note: Localizes to mitotic spindles.By similarity

    GO - Cellular componenti

    1. cytoplasmic dynein complex Source: UniProtKB
    2. Golgi apparatus Source: UniProtKB-SubCell
    3. microtubule Source: UniProtKB-KW
    4. photoreceptor inner segment Source: UniProtKB
    5. spindle Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Dynein, Golgi apparatus, Microtubule

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 113113Dynein light chain Tctex-type 1PRO_0000195153Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity

    Post-translational modificationi

    Phosphorylated by BMPR2. The phosphorylation status is proposed to regulate the association with the cytoplasmic dynein complex and may have role in cytoplasmic dynein cargo release By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP51807.
    PaxDbiP51807.
    PRIDEiP51807.

    PTM databases

    PhosphoSiteiP51807.

    Expressioni

    Tissue specificityi

    High level in testis (germ cell-specific). Expressed in sperm (at protein level). 200-fold lower in liver, brain, heart, spleen, and kidney. Levels in thymus and two embryonal carcinoma cell lines were several-fold higher than this low constitutive level.1 Publication

    Developmental stagei

    First abundantly expressed at the pachytene stage of meiosis and persists throughout spermatogenesis.

    Gene expression databases

    BgeeiP51807.
    CleanExiMM_DYNLT1.
    GenevestigatoriP51807.

    Interactioni

    Subunit structurei

    Homodimer Probable. The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs); the composition seems to vary in respect to the IC, LIC and LC composition. The heavy chain homodimer serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. The ICs and LICs bind directly to the HC dimer and the LCs assemble on the IC dimer. DYNLT1 and DYNLT3 compete for association with dynein IC (DYNC1I1 or DYNC1I2). Self-associates. Interacts with RHO By similarity. Interacts with DYNC1I1 and DYNC1I2. Interacts with DOC2A, DOC2B and SCN10A. Interacts with PVR. Interacts with SVIL isoform 2. Interacts with GNB1; the interaction occurs in presence of guanine nucleotide-binding protein G(T) subunit gamma; the interaction diminishes the association of DYNLT1 with dynein IC (DYNC1I1 or DYNC1I2). Interacts with GNB2, GNB3 and GNB5; the interactions occur in presence of guanine nucleotide-binding protein G(T) subunit gamma By similarity.By similarityCurated

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    FynP396883EBI-642797,EBI-524514
    SVILO463854EBI-642797,EBI-6995105From a different organism.

    Protein-protein interaction databases

    BioGridi204082. 2 interactions.
    IntActiP51807. 9 interactions.
    MINTiMINT-7901686.

    Structurei

    3D structure databases

    ProteinModelPortaliP51807.
    SMRiP51807. Positions 12-113.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni41 – 11373Interaction with GNB1By similarityAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG278055.
    GeneTreeiENSGT00390000013998.
    HOGENOMiHOG000255148.
    HOVERGENiHBG001217.
    InParanoidiQ5M8S6.
    KOiK10420.
    OMAiSCFWDNS.
    OrthoDBiEOG7PK918.
    PhylomeDBiP51807.
    TreeFamiTF313904.

    Family and domain databases

    InterProiIPR005334. Tctex.
    [Graphical view]
    PANTHERiPTHR21255. PTHR21255. 1 hit.
    PfamiPF03645. Tctex-1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P51807-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEDFQASEET AFVVDEVSSI VKEAIESAIG GNAYQHSKVN QWTTNVLEQT    50
    LSQLTKLGRP FKYIVTCVIM QKNGAGLHSA SSCFWDSSTD GSCTVRWENK 100
    TMYCIVSTFG LSI 113
    Length:113
    Mass (Da):12,483
    Last modified:October 1, 1996 - v1
    Checksum:i92DF96F933DB7AF1
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti21 – 211V → A.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M25825 mRNA. Translation: AAA40408.1.
    AK133788 mRNA. Translation: BAE21842.1.
    AK153971 mRNA. Translation: BAE32288.1.
    AK168478 mRNA. Translation: BAE40367.1.
    BC043018 mRNA. Translation: AAH43018.1.
    BC087868 mRNA. Translation: AAH87868.1.
    CCDSiCCDS49936.1.
    PIRiA32995.
    RefSeqiNP_001160099.1. NM_001166627.1.
    NP_001160101.1. NM_001166629.2.
    NP_001160102.1. NM_001166630.1.
    NP_033368.1. NM_009342.2.
    UniGeneiMm.1948.
    Mm.381893.
    Mm.428775.
    Mm.457381.
    Mm.457987.
    Mm.458102.
    Mm.458208.
    Mm.463722.

    Genome annotation databases

    EnsembliENSMUST00000092966; ENSMUSP00000090644; ENSMUSG00000000579.
    ENSMUST00000169415; ENSMUSP00000127990; ENSMUSG00000092074.
    ENSMUST00000179554; ENSMUSP00000135978; ENSMUSG00000095677.
    ENSMUST00000179569; ENSMUSP00000137171; ENSMUSG00000096255.
    GeneIDi100040531.
    100040563.
    100310872.
    21648.
    KEGGimmu:100040531.
    mmu:100040563.
    mmu:100310872.
    mmu:21648.
    UCSCiuc008agl.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M25825 mRNA. Translation: AAA40408.1 .
    AK133788 mRNA. Translation: BAE21842.1 .
    AK153971 mRNA. Translation: BAE32288.1 .
    AK168478 mRNA. Translation: BAE40367.1 .
    BC043018 mRNA. Translation: AAH43018.1 .
    BC087868 mRNA. Translation: AAH87868.1 .
    CCDSi CCDS49936.1.
    PIRi A32995.
    RefSeqi NP_001160099.1. NM_001166627.1.
    NP_001160101.1. NM_001166629.2.
    NP_001160102.1. NM_001166630.1.
    NP_033368.1. NM_009342.2.
    UniGenei Mm.1948.
    Mm.381893.
    Mm.428775.
    Mm.457381.
    Mm.457987.
    Mm.458102.
    Mm.458208.
    Mm.463722.

    3D structure databases

    ProteinModelPortali P51807.
    SMRi P51807. Positions 12-113.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204082. 2 interactions.
    IntActi P51807. 9 interactions.
    MINTi MINT-7901686.

    PTM databases

    PhosphoSitei P51807.

    Proteomic databases

    MaxQBi P51807.
    PaxDbi P51807.
    PRIDEi P51807.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000092966 ; ENSMUSP00000090644 ; ENSMUSG00000000579 .
    ENSMUST00000169415 ; ENSMUSP00000127990 ; ENSMUSG00000092074 .
    ENSMUST00000179554 ; ENSMUSP00000135978 ; ENSMUSG00000095677 .
    ENSMUST00000179569 ; ENSMUSP00000137171 ; ENSMUSG00000096255 .
    GeneIDi 100040531.
    100040563.
    100310872.
    21648.
    KEGGi mmu:100040531.
    mmu:100040563.
    mmu:100310872.
    mmu:21648.
    UCSCi uc008agl.2. mouse.

    Organism-specific databases

    CTDi 100040531.
    100040563.
    100310872.
    21648.
    MGIi MGI:98643. Dynlt1.

    Phylogenomic databases

    eggNOGi NOG278055.
    GeneTreei ENSGT00390000013998.
    HOGENOMi HOG000255148.
    HOVERGENi HBG001217.
    InParanoidi Q5M8S6.
    KOi K10420.
    OMAi SCFWDNS.
    OrthoDBi EOG7PK918.
    PhylomeDBi P51807.
    TreeFami TF313904.

    Miscellaneous databases

    NextBioi 300916.
    PROi P51807.
    SOURCEi Search...

    Gene expression databases

    Bgeei P51807.
    CleanExi MM_DYNLT1.
    Genevestigatori P51807.

    Family and domain databases

    InterProi IPR005334. Tctex.
    [Graphical view ]
    PANTHERi PTHR21255. PTHR21255. 1 hit.
    Pfami PF03645. Tctex-1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "tctex-1: a candidate gene family for a mouse t complex sterility locus."
      Lader E., Ha H.-S., O'Neill M., Artzt K., Bennett D.
      Cell 58:969-979(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Testis.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
      Tissue: Liver and Thymus.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain and Testis.
    4. "Identification of the t complex-encoded cytoplasmic dynein light chain tctex1 in inner arm I1 supports the involvement of flagellar dyneins in meiotic drive."
      Harrison A., Olds-Clarke P., King S.M.
      J. Cell Biol. 140:1137-1147(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, POSSIBLE FUNCTION IN AXONEMAL DYNEIN.
    5. "Receptor-independent activators of heterotrimeric G-protein signaling pathways."
      Takesono A., Cismowski M.J., Ribas C., Bernard M., Chung P., Hazard S. III, Duzic E., Lanier S.M.
      J. Biol. Chem. 274:33202-33205(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN RECEPTOR-INDEPENDENT ACTIVATION OF G-PROTEIN SIGNALING, ASSOCIATION WITH GBETA-GAMMA.
    6. "Structure of Tctex-1 and its interaction with cytoplasmic dynein intermediate chain."
      Mok Y.K., Lo K.W., Zhang M.
      J. Biol. Chem. 276:14067-14074(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH DYNC1I1, SUBUNIT.
    7. "The Tctex1/Tctex2 class of dynein light chains. Dimerization, differential expression, and interaction with the LC8 protein family."
      DiBella L.M., Benashski S.E., Tedford H.W., Harrison A., Patel-King R.S., King S.M.
      J. Biol. Chem. 276:14366-14373(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SELF-ASSOCIATION.
    8. "Lfc and Tctex-1 regulate the genesis of neurons from cortical precursor cells."
      Gauthier-Fisher A., Lin D.C., Greeve M., Kaplan D.R., Rottapel R., Miller F.D.
      Nat. Neurosci. 12:735-744(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN NEUROGENESIS.

    Entry informationi

    Entry nameiDYLT1_MOUSE
    AccessioniPrimary (citable) accession number: P51807
    Secondary accession number(s): Q5M8S6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3