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Protein

Chloride channel protein ClC-Kb

Gene

Clcnkb

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport. May be important in urinary concentrating mechanisms.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi259CalciumBy similarity1
Metal bindingi261CalciumBy similarity1
Metal bindingi278CalciumBy similarity1
Metal bindingi281CalciumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • chloride transport Source: RGD
  • transepithelial chloride transport Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Calcium, Chloride, Metal-binding

Protein family/group databases

TCDBi2.A.49.2.5. the chloride carrier/channel (clc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Chloride channel protein ClC-Kb
Short name:
Chloride channel Kb
Alternative name(s):
ClC-K2
Gene namesi
Name:Clcnkb
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi628639. Clcnkb.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 51CytoplasmicSequence analysisAdd BLAST51
Transmembranei52 – 72HelicalSequence analysisAdd BLAST21
Transmembranei94 – 114HelicalSequence analysisAdd BLAST21
Transmembranei161 – 181HelicalSequence analysisAdd BLAST21
Transmembranei202 – 222HelicalSequence analysisAdd BLAST21
Transmembranei236 – 256HelicalSequence analysisAdd BLAST21
Transmembranei275 – 295HelicalSequence analysisAdd BLAST21
Transmembranei325 – 345HelicalSequence analysisAdd BLAST21
Transmembranei410 – 430HelicalSequence analysisAdd BLAST21
Transmembranei434 – 454HelicalSequence analysisAdd BLAST21
Transmembranei458 – 478HelicalSequence analysisAdd BLAST21
Transmembranei486 – 506HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000944621 – 687Chloride channel protein ClC-KbAdd BLAST687

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi364N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP51802.
PRIDEiP51802.

PTM databases

PhosphoSitePlusiP51802.

Expressioni

Tissue specificityi

Expressed predominantly in the kidney. Expressed in all segments of the nephron examined, including the S2 segment and the glomerulus.

Inductioni

Expression is consitently weaker in the absence of BSND protein expression than it is in its presence. The half-life with BSND protein is much longer than that without it. Rapidly degraded without BSND protein, exhibiting a very short half-life of less than 1 hour.1 Publication

Interactioni

Subunit structurei

Interacts with BSND.2 Publications

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013550.

Structurei

3D structure databases

ProteinModelPortaliP51802.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini551 – 609CBS 1PROSITE-ProRule annotationAdd BLAST59
Domaini626 – 687CBS 2PROSITE-ProRule annotationAdd BLAST62

Sequence similaritiesi

Contains 2 CBS domains.PROSITE-ProRule annotation

Keywords - Domaini

CBS domain, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0476. Eukaryota.
COG0038. LUCA.
HOGENOMiHOG000231297.
HOVERGENiHBG005332.
InParanoidiP51802.
KOiK05018.
PhylomeDBiP51802.

Family and domain databases

Gene3Di1.10.3080.10. 2 hits.
InterProiIPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
IPR002250. Cl_channel-K.
[Graphical view]
PfamiPF00571. CBS. 1 hit.
PF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
PR01119. CLCHANNELKDY.
SMARTiSM00116. CBS. 1 hit.
[Graphical view]
SUPFAMiSSF81340. SSF81340. 2 hits.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P51802-1) [UniParc]FASTAAdd to basket
Also known as: ClC-K2L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEEIVGLREG SPRKPVPLQE LWRPCPRIRR NIQGSLEWLK ERLFRVGEDW
60 70 80 90 100
YFLVALGVLM ALISYAMNFA IGRVVRAHKW LYREIGDGHL LRYLSWTVYP
110 120 130 140 150
VALLSFSSGF SQSITPSSGG SGIPEVKTIL TGVILEDYLD IKNFGAKVVG
160 170 180 190 200
LSCTLATGST IFLGKLGPFV HLSVMIAAYL GRVRTKTVGE PENKTKEMEL
210 220 230 240 250
LAAGAAVGVA TVFAAPISGV LFSIEVMSSH FSVWDYWRGF FAATCGAFMF
260 270 280 290 300
HLLAVFNSEQ ETITSIYKTS FPVDIPFDLP EIFFFVALGA ICGILSCGYN
310 320 330 340 350
YCQRTSLFFL KSNGFTSKLL ATSKPLYSAL AAVVLASITY PPGVGRFMAS
360 370 380 390 400
RLSMSEYLET LFDNNSWALM TKNSSPPWSA EPDPQNLWLE WCHPQMTVFG
410 420 430 440 450
TLVFFLVMKF WMLILATTIP IPAGYFLPIF VYGAAIGRLF GEVLSLAFPE
460 470 480 490 500
GIVAGGKVSP IMPGAYALAG AAAFSGAVTH TLSTALLAFE VSGQIVHALP
510 520 530 540 550
VLMAVLAANA ICQSYQPSFY DGTIIVKKLP YLPWIRGRKI GSHLVTVGHF
560 570 580 590 600
MNCTLTTLAK DMPLEEVIKV VISTDVTQYP LVETTESQVL VGIVKRTHLV
610 620 630 640 650
QSLHTDSASW APGQQPCLQD ILANGCPTQP VTLQLSTETS LHETHNLFEL
660 670 680
LNLQLLFVTS RGRAVGSVSW VELKKAISTL TNPPAPK
Length:687
Mass (Da):75,218
Last modified:October 1, 1996 - v1
Checksum:i35B0BD7735397880
GO
Isoform 2 (identifier: P51802-2) [UniParc]FASTAAdd to basket
Also known as: ClC-K2S

The sequence of this isoform differs from the canonical sequence as follows:
     77-131: Missing.

Show »
Length:632
Mass (Da):69,211
Checksum:i7C62CDD5822EF0D6
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00104977 – 131Missing in isoform 2. 1 PublicationAdd BLAST55

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z30663 mRNA. Translation: CAA83143.1.
D26111 mRNA. Translation: BAA05106.1.
D26111 mRNA. Translation: BAA05107.1.
PIRiB57713.
RefSeqiNP_775126.1. NM_173103.1. [P51802-1]
UniGeneiRn.10339.

Genome annotation databases

GeneIDi79430.
KEGGirno:79430.
UCSCiRGD:628639. rat. [P51802-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z30663 mRNA. Translation: CAA83143.1.
D26111 mRNA. Translation: BAA05106.1.
D26111 mRNA. Translation: BAA05107.1.
PIRiB57713.
RefSeqiNP_775126.1. NM_173103.1. [P51802-1]
UniGeneiRn.10339.

3D structure databases

ProteinModelPortaliP51802.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013550.

Protein family/group databases

TCDBi2.A.49.2.5. the chloride carrier/channel (clc) family.

PTM databases

PhosphoSitePlusiP51802.

Proteomic databases

PaxDbiP51802.
PRIDEiP51802.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi79430.
KEGGirno:79430.
UCSCiRGD:628639. rat. [P51802-1]

Organism-specific databases

CTDi1188.
RGDi628639. Clcnkb.

Phylogenomic databases

eggNOGiKOG0476. Eukaryota.
COG0038. LUCA.
HOGENOMiHOG000231297.
HOVERGENiHBG005332.
InParanoidiP51802.
KOiK05018.
PhylomeDBiP51802.

Miscellaneous databases

PROiP51802.

Family and domain databases

Gene3Di1.10.3080.10. 2 hits.
InterProiIPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
IPR002250. Cl_channel-K.
[Graphical view]
PfamiPF00571. CBS. 1 hit.
PF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
PR01119. CLCHANNELKDY.
SMARTiSM00116. CBS. 1 hit.
[Graphical view]
SUPFAMiSSF81340. SSF81340. 2 hits.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLCKB_RAT
AccessioniPrimary (citable) accession number: P51802
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.