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Protein

Chloride channel protein ClC-Kb

Gene

Clcnkb

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport. May be important in urinary concentrating mechanisms.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi259 – 2591CalciumBy similarity
Metal bindingi261 – 2611CalciumBy similarity
Metal bindingi278 – 2781CalciumBy similarity
Metal bindingi281 – 2811CalciumBy similarity

GO - Molecular functioni

GO - Biological processi

  • chloride transmembrane transport Source: GOC
  • chloride transport Source: RGD
  • transepithelial chloride transport Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Calcium, Chloride, Metal-binding

Protein family/group databases

TCDBi2.A.49.2.5. the chloride carrier/channel (clc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Chloride channel protein ClC-Kb
Short name:
Chloride channel Kb
Alternative name(s):
ClC-K2
Gene namesi
Name:Clcnkb
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi628639. Clcnkb.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5151CytoplasmicSequence analysisAdd
BLAST
Transmembranei52 – 7221HelicalSequence analysisAdd
BLAST
Transmembranei94 – 11421HelicalSequence analysisAdd
BLAST
Transmembranei161 – 18121HelicalSequence analysisAdd
BLAST
Transmembranei202 – 22221HelicalSequence analysisAdd
BLAST
Transmembranei236 – 25621HelicalSequence analysisAdd
BLAST
Transmembranei275 – 29521HelicalSequence analysisAdd
BLAST
Transmembranei325 – 34521HelicalSequence analysisAdd
BLAST
Transmembranei410 – 43021HelicalSequence analysisAdd
BLAST
Transmembranei434 – 45421HelicalSequence analysisAdd
BLAST
Transmembranei458 – 47821HelicalSequence analysisAdd
BLAST
Transmembranei486 – 50621HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 687687Chloride channel protein ClC-KbPRO_0000094462Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi364 – 3641N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP51802.
PRIDEiP51802.

PTM databases

PhosphoSiteiP51802.

Expressioni

Tissue specificityi

Expressed predominantly in the kidney. Expressed in all segments of the nephron examined, including the S2 segment and the glomerulus.

Inductioni

Expression is consitently weaker in the absence of BSND protein expression than it is in its presence. The half-life with BSND protein is much longer than that without it. Rapidly degraded without BSND protein, exhibiting a very short half-life of less than 1 hour.1 Publication

Interactioni

Subunit structurei

Interacts with BSND.2 Publications

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013550.

Structurei

3D structure databases

ProteinModelPortaliP51802.
SMRiP51802. Positions 542-687.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini551 – 60959CBS 1PROSITE-ProRule annotationAdd
BLAST
Domaini626 – 68762CBS 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 CBS domains.PROSITE-ProRule annotation

Keywords - Domaini

CBS domain, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0476. Eukaryota.
COG0038. LUCA.
HOGENOMiHOG000231297.
HOVERGENiHBG005332.
InParanoidiP51802.
KOiK05018.
PhylomeDBiP51802.

Family and domain databases

Gene3Di1.10.3080.10. 2 hits.
InterProiIPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
IPR002250. Cl_channel-K.
[Graphical view]
PfamiPF00571. CBS. 1 hit.
PF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
PR01119. CLCHANNELKDY.
SMARTiSM00116. CBS. 1 hit.
[Graphical view]
SUPFAMiSSF81340. SSF81340. 2 hits.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P51802-1) [UniParc]FASTAAdd to basket

Also known as: ClC-K2L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEEIVGLREG SPRKPVPLQE LWRPCPRIRR NIQGSLEWLK ERLFRVGEDW
60 70 80 90 100
YFLVALGVLM ALISYAMNFA IGRVVRAHKW LYREIGDGHL LRYLSWTVYP
110 120 130 140 150
VALLSFSSGF SQSITPSSGG SGIPEVKTIL TGVILEDYLD IKNFGAKVVG
160 170 180 190 200
LSCTLATGST IFLGKLGPFV HLSVMIAAYL GRVRTKTVGE PENKTKEMEL
210 220 230 240 250
LAAGAAVGVA TVFAAPISGV LFSIEVMSSH FSVWDYWRGF FAATCGAFMF
260 270 280 290 300
HLLAVFNSEQ ETITSIYKTS FPVDIPFDLP EIFFFVALGA ICGILSCGYN
310 320 330 340 350
YCQRTSLFFL KSNGFTSKLL ATSKPLYSAL AAVVLASITY PPGVGRFMAS
360 370 380 390 400
RLSMSEYLET LFDNNSWALM TKNSSPPWSA EPDPQNLWLE WCHPQMTVFG
410 420 430 440 450
TLVFFLVMKF WMLILATTIP IPAGYFLPIF VYGAAIGRLF GEVLSLAFPE
460 470 480 490 500
GIVAGGKVSP IMPGAYALAG AAAFSGAVTH TLSTALLAFE VSGQIVHALP
510 520 530 540 550
VLMAVLAANA ICQSYQPSFY DGTIIVKKLP YLPWIRGRKI GSHLVTVGHF
560 570 580 590 600
MNCTLTTLAK DMPLEEVIKV VISTDVTQYP LVETTESQVL VGIVKRTHLV
610 620 630 640 650
QSLHTDSASW APGQQPCLQD ILANGCPTQP VTLQLSTETS LHETHNLFEL
660 670 680
LNLQLLFVTS RGRAVGSVSW VELKKAISTL TNPPAPK
Length:687
Mass (Da):75,218
Last modified:October 1, 1996 - v1
Checksum:i35B0BD7735397880
GO
Isoform 2 (identifier: P51802-2) [UniParc]FASTAAdd to basket

Also known as: ClC-K2S

The sequence of this isoform differs from the canonical sequence as follows:
     77-131: Missing.

Show »
Length:632
Mass (Da):69,211
Checksum:i7C62CDD5822EF0D6
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei77 – 13155Missing in isoform 2. 1 PublicationVSP_001049Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z30663 mRNA. Translation: CAA83143.1.
D26111 mRNA. Translation: BAA05106.1.
D26111 mRNA. Translation: BAA05107.1.
PIRiB57713.
RefSeqiNP_775126.1. NM_173103.1. [P51802-1]
UniGeneiRn.10339.

Genome annotation databases

GeneIDi79430.
KEGGirno:79430.
UCSCiRGD:628639. rat. [P51802-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z30663 mRNA. Translation: CAA83143.1.
D26111 mRNA. Translation: BAA05106.1.
D26111 mRNA. Translation: BAA05107.1.
PIRiB57713.
RefSeqiNP_775126.1. NM_173103.1. [P51802-1]
UniGeneiRn.10339.

3D structure databases

ProteinModelPortaliP51802.
SMRiP51802. Positions 542-687.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013550.

Protein family/group databases

TCDBi2.A.49.2.5. the chloride carrier/channel (clc) family.

PTM databases

PhosphoSiteiP51802.

Proteomic databases

PaxDbiP51802.
PRIDEiP51802.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi79430.
KEGGirno:79430.
UCSCiRGD:628639. rat. [P51802-1]

Organism-specific databases

CTDi1188.
RGDi628639. Clcnkb.

Phylogenomic databases

eggNOGiKOG0476. Eukaryota.
COG0038. LUCA.
HOGENOMiHOG000231297.
HOVERGENiHBG005332.
InParanoidiP51802.
KOiK05018.
PhylomeDBiP51802.

Miscellaneous databases

PROiP51802.

Family and domain databases

Gene3Di1.10.3080.10. 2 hits.
InterProiIPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
IPR002250. Cl_channel-K.
[Graphical view]
PfamiPF00571. CBS. 1 hit.
PF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
PR01119. CLCHANNELKDY.
SMARTiSM00116. CBS. 1 hit.
[Graphical view]
SUPFAMiSSF81340. SSF81340. 2 hits.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Two highly homologous members of the ClC chloride channel family in both rat and human kidney."
    Kieferle S., Fong P., Bens M., Vandewalle A., Jentsch T.
    Proc. Natl. Acad. Sci. U.S.A. 91:6943-6947(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Kidney.
  2. "Two isoforms of a chloride channel predominantly expressed in thick ascending limb of Henle's loop and collecting ducts of rat kidney."
    Adachi S., Uchida S., Hata M., Hirose M., Marumo F., Sasaki S., Ito H.
    J. Biol. Chem. 269:17677-17683(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    Tissue: Kidney.
  3. "Barttin increases surface expression and changes current properties of ClC-K channels."
    Waldegger S., Jeck N., Barth P., Peters M., Vitzthum H., Wolf K., Kurtz A., Konrad M., Seyberth H.W.
    Pflugers Arch. 444:411-418(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BSND.
  4. "Molecular mechanisms of Bartter syndrome caused by mutations in the BSND gene."
    Hayama A., Rai T., Sasaki S., Uchida S.
    Histochem. Cell Biol. 119:485-493(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, SUBCELLULAR LOCATION, INDUCTION.

Entry informationi

Entry nameiCLCKB_RAT
AccessioniPrimary (citable) accession number: P51802
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 8, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.