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Protein

H(+)/Cl(-) exchange transporter 7

Gene

Clcn7

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Slowly voltage-gated channel mediating the exchange of chloride ions against protons. Functions as antiporter and contributes to the acidification of the lysosome lumen.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei202ChlorideBy similarity1
Sitei245Mediates proton transfer from the outer aqueous phase to the interior of the protein; involved in linking H(+) and Cl(-) transportBy similarity1
Sitei312Mediates proton transfer from the protein to the inner aqueous phaseBy similarity1
Binding sitei512Chloride; via amide nitrogenBy similarity1
Binding sitei600ChlorideBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi656 – 658ATPBy similarity3
Nucleotide bindingi781 – 784ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

  • response to pH Source: RGD
Complete GO annotation...

Keywords - Biological processi

Antiport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Chloride, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-2672351. Stimuli-sensing channels.

Names & Taxonomyi

Protein namesi
Recommended name:
H(+)/Cl(-) exchange transporter 7
Alternative name(s):
Chloride channel 7 alpha subunit
Chloride channel protein 7
Short name:
ClC-7
Gene namesi
Name:Clcn7
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi61836. Clcn7.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 124CytoplasmicBy similarityAdd BLAST124
Transmembranei125 – 157HelicalBy similarityAdd BLAST33
Transmembranei172 – 195HelicalBy similarityAdd BLAST24
Intramembranei204 – 211HelicalBy similarity8
Transmembranei221 – 239HelicalBy similarityAdd BLAST19
Transmembranei245 – 262HelicalBy similarityAdd BLAST18
Intramembranei286 – 298HelicalBy similarityAdd BLAST13
Intramembranei302 – 310HelicalBy similarity9
Transmembranei320 – 339HelicalBy similarityAdd BLAST20
Transmembranei373 – 403HelicalBy similarityAdd BLAST31
Transmembranei408 – 430HelicalBy similarityAdd BLAST23
Transmembranei485 – 505HelicalBy similarityAdd BLAST21
Transmembranei510 – 533HelicalBy similarityAdd BLAST24
Intramembranei543 – 557HelicalBy similarityAdd BLAST15
Intramembranei558 – 560Note=Loop between two helicesBy similarity3
Intramembranei561 – 572HelicalBy similarityAdd BLAST12
Intramembranei573 – 576Note=Loop between two helicesBy similarity4
Transmembranei577 – 595HelicalBy similarityAdd BLAST19
Topological domaini596 – 803CytoplasmicBy similarityAdd BLAST208

GO - Cellular componenti

  • cytoplasmic vesicle Source: RGD
  • integral component of membrane Source: UniProtKB-KW
  • lysosomal membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000944541 – 803H(+)/Cl(-) exchange transporter 7Add BLAST803

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei9PhosphoserineBy similarity1
Modified residuei799PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP51799.
PRIDEiP51799.

PTM databases

iPTMnetiP51799.
PhosphoSitePlusiP51799.

Expressioni

Tissue specificityi

Brain, testis, muscle and kidney.

Gene expression databases

BgeeiENSRNOG00000016976.
GenevisibleiP51799. RN.

Interactioni

Subunit structurei

Chloride channel 7 are heteromers of alpha (CLCN7) and beta (OSTM1) subunits.By similarity

Protein-protein interaction databases

IntActiP51799. 2 interactors.
MINTiMINT-4592777.
STRINGi10116.ENSRNOP00000023615.

Structurei

3D structure databases

ProteinModelPortaliP51799.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini629 – 693CBS 1PROSITE-ProRule annotationAdd BLAST65
Domaini739 – 797CBS 2PROSITE-ProRule annotationAdd BLAST59

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi201 – 205Selectivity filter part_1By similarity5
Motifi243 – 247Selectivity filter part_2By similarity5
Motifi510 – 514Selectivity filter part_3By similarity5

Sequence similaritiesi

Contains 2 CBS domains.PROSITE-ProRule annotation

Keywords - Domaini

CBS domain, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0474. Eukaryota.
COG0038. LUCA.
GeneTreeiENSGT00760000119109.
HOGENOMiHOG000231081.
HOVERGENiHBG050985.
InParanoidiP51799.
KOiK05016.
OMAiFPVVEHA.
OrthoDBiEOG091G02SB.
PhylomeDBiP51799.
TreeFamiTF313867.

Family and domain databases

Gene3Di1.10.3080.10. 2 hits.
InterProiIPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
IPR002249. Cl_channel-7.
[Graphical view]
PfamiPF00571. CBS. 2 hits.
PF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
PR01118. CLCHANNEL7.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
SUPFAMiSSF81340. SSF81340. 2 hits.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P51799-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANVSKKVSW SGRDRDDEEG APLLRRTGQP DEETPLLNGA GPGARQSHSA
60 70 80 90 100
LFRIGQMNNV ELDDELLDPE VDPPHTFPKE IPHNEKLLSL KYESLDYDNS
110 120 130 140 150
ENQLFLEEER RINHTAFRTV EIKRWVICAL IGILTGLVAC FIDIVVENLA
160 170 180 190 200
GLKYRVIKDN IDKFTEKGGL SFSLLLWATL NSAFVLVGSV IVAFIEPVAA
210 220 230 240 250
GSGIPQIKCF LNGVKIPHVV RLKTLVIKVS GVILSVVGGL AVGKEGPMIH
260 270 280 290 300
SGSVIAAGIS QGRSTSLKRD FKIFEYFRRD TEKRDFVSAG AAAGVSAAFG
310 320 330 340 350
APVGGVLFSL EEGASFWNQF LTWRIFFASM ISTFTLNFVL SIYHGNMWDL
360 370 380 390 400
SSPGLINFGR FDSEKMAYTI HEIPVFIAMG VVGGILGAVF NALNYWLTMF
410 420 430 440 450
RIRYIHRPCL QVIEAMLVAA VTATVAFVLI YSSRDCQPLQ GSSMSYPLQL
460 470 480 490 500
FCADGEYNSM AAAFFNTPEK SVVSLFHDPP GSYNPMTLGL FTLVYFFLAC
510 520 530 540 550
WTYGLTVSAG VFIPSLLIGA AWGRLFGISM SYLTGAAIWA DPGKYALMGA
560 570 580 590 600
AAQLGGIVRM TLSLTVIMME ATSNVTYGFP IMLVLMTAKI VGDVFIEGLY
610 620 630 640 650
DMHIQLQSVP FLHWEAPVTS HSLTAREVMS TPVTCLRRRE KVGIIVDVLS
660 670 680 690 700
DTASNHNGFP VVEDVGDTQP ARLQGLILRS QLIVLLKHKV FVERSNMGLV
710 720 730 740 750
QRRLRLKDFR DAYPRFPPIQ SIHVSQDERE CTMDLSEFMN PSPYTVPQEA
760 770 780 790 800
SLPRVFKLFR ALGLRHLVVV DNHNQVVGLV TRKDLARYRL GKGGLEELSL

AQT
Length:803
Mass (Da):88,731
Last modified:October 1, 1996 - v1
Checksum:iA7D6AA3D21DA6FED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z67744 mRNA. Translation: CAA91557.1.
PIRiS68426.
RefSeqiNP_113756.1. NM_031568.2.
UniGeneiRn.10338.

Genome annotation databases

EnsembliENSRNOT00000023615; ENSRNOP00000023615; ENSRNOG00000016976.
GeneIDi29233.
KEGGirno:29233.
UCSCiRGD:61836. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z67744 mRNA. Translation: CAA91557.1.
PIRiS68426.
RefSeqiNP_113756.1. NM_031568.2.
UniGeneiRn.10338.

3D structure databases

ProteinModelPortaliP51799.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP51799. 2 interactors.
MINTiMINT-4592777.
STRINGi10116.ENSRNOP00000023615.

PTM databases

iPTMnetiP51799.
PhosphoSitePlusiP51799.

Proteomic databases

PaxDbiP51799.
PRIDEiP51799.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000023615; ENSRNOP00000023615; ENSRNOG00000016976.
GeneIDi29233.
KEGGirno:29233.
UCSCiRGD:61836. rat.

Organism-specific databases

CTDi1186.
RGDi61836. Clcn7.

Phylogenomic databases

eggNOGiKOG0474. Eukaryota.
COG0038. LUCA.
GeneTreeiENSGT00760000119109.
HOGENOMiHOG000231081.
HOVERGENiHBG050985.
InParanoidiP51799.
KOiK05016.
OMAiFPVVEHA.
OrthoDBiEOG091G02SB.
PhylomeDBiP51799.
TreeFamiTF313867.

Enzyme and pathway databases

ReactomeiR-RNO-2672351. Stimuli-sensing channels.

Miscellaneous databases

PROiP51799.

Gene expression databases

BgeeiENSRNOG00000016976.
GenevisibleiP51799. RN.

Family and domain databases

Gene3Di1.10.3080.10. 2 hits.
InterProiIPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
IPR002249. Cl_channel-7.
[Graphical view]
PfamiPF00571. CBS. 2 hits.
PF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
PR01118. CLCHANNEL7.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
SUPFAMiSSF81340. SSF81340. 2 hits.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLCN7_RAT
AccessioniPrimary (citable) accession number: P51799
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons. The presence of conserved gating glutamate residues is typical for family members that function as antiporters (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.