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Protein

H(+)/Cl(-) exchange transporter 5

Gene

Clcn5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons. Important for normal acidification of the endosome lumen. May play an important role in renal tubular function (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei168ChlorideBy similarity1
Sitei211Mediates proton transfer from the outer aqueous phase to the interior of the protein; involved in linking H(+) and Cl(-) transportBy similarity1
Sitei268Mediates proton transfer from the protein to the inner aqueous phaseBy similarity1
Binding sitei455Chloride; via amide nitrogenBy similarity1
Binding sitei558ChlorideBy similarity1
Binding sitei596ATP; via amide nitrogen and carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi617 – 619ATPBy similarity3
Nucleotide bindingi724 – 727ATPBy similarity4

GO - Molecular functioni

  • antiporter activity Source: UniProtKB-KW
  • ATP binding Source: UniProtKB-KW
  • voltage-gated chloride channel activity Source: RGD

GO - Biological processi

  • chloride transport Source: RGD
Complete GO annotation...

Keywords - Biological processi

Antiport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Chloride, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-2672351. Stimuli-sensing channels.

Names & Taxonomyi

Protein namesi
Recommended name:
H(+)/Cl(-) exchange transporter 5
Alternative name(s):
Chloride channel protein 5
Short name:
ClC-5
Chloride transporter ClC-5
Gene namesi
Name:Clcn5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi2362. Clcn5.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 54CytoplasmicBy similarityAdd BLAST54
Transmembranei55 – 92HelicalBy similarityAdd BLAST38
Transmembranei138 – 161HelicalBy similarityAdd BLAST24
Intramembranei170 – 177HelicalBy similarity8
Transmembranei186 – 205HelicalBy similarityAdd BLAST20
Transmembranei211 – 230HelicalBy similarityAdd BLAST20
Intramembranei242 – 254HelicalBy similarityAdd BLAST13
Intramembranei258 – 266HelicalBy similarity9
Transmembranei278 – 296HelicalBy similarityAdd BLAST19
Transmembranei319 – 344HelicalBy similarityAdd BLAST26
Transmembranei352 – 372HelicalBy similarityAdd BLAST21
Transmembranei428 – 448HelicalBy similarityAdd BLAST21
Transmembranei453 – 472HelicalBy similarityAdd BLAST20
Intramembranei500 – 514HelicalBy similarityAdd BLAST15
Intramembranei515 – 517Note=Loop between two helicesBy similarity3
Intramembranei518 – 529HelicalBy similarityAdd BLAST12
Intramembranei530 – 534Note=Loop between two helicesBy similarity5
Transmembranei535 – 552HelicalBy similarityAdd BLAST18
Topological domaini553 – 746CytoplasmicBy similarityAdd BLAST194

GO - Cellular componenti

  • apical part of cell Source: UniProtKB
  • endosome Source: RGD
  • endosome membrane Source: UniProtKB-SubCell
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: UniProtKB
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000944481 – 746H(+)/Cl(-) exchange transporter 5Add BLAST746

Post-translational modificationi

Ubiquitinated by NEDD4L in the presence of albumin; which promotes endocytosis and proteasomal degradation.By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiP51796.
PRIDEiP51796.

PTM databases

PhosphoSitePlusiP51796.

Expressioni

Tissue specificityi

Kidney specific.

Gene expression databases

BgeeiENSRNOG00000002862.
ExpressionAtlasiP51796. differential.
GenevisibleiP51796. RN.

Interactioni

Subunit structurei

Interacts with NEDD4 and NEDD4L.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003895.

Structurei

3D structure databases

ProteinModelPortaliP51796.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini586 – 650CBS 1PROSITE-ProRule annotationAdd BLAST65
Domaini682 – 741CBS 2PROSITE-ProRule annotationAdd BLAST60

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi167 – 171Selectivity filter part_1By similarity5
Motifi209 – 213Selectivity filter part_2By similarity5
Motifi453 – 457Selectivity filter part_3By similarity5

Sequence similaritiesi

Contains 2 CBS domains.PROSITE-ProRule annotation

Keywords - Domaini

CBS domain, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0475. Eukaryota.
COG0038. LUCA.
GeneTreeiENSGT00760000119109.
HOGENOMiHOG000164493.
HOVERGENiHBG050984.
InParanoidiP51796.
KOiK05012.
PhylomeDBiP51796.

Family and domain databases

Gene3Di1.10.3080.10. 3 hits.
InterProiIPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
IPR002247. Cl_channel-5.
[Graphical view]
PfamiPF00571. CBS. 2 hits.
PF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
PR01116. CLCHANNEL5.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
SUPFAMiSSF81340. SSF81340. 3 hits.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P51796-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFLEEPIPG VGTYDDFNTI DWVREKSRDR DRHREITNKS KESTWALIHS
60 70 80 90 100
VSDAFSGWLL MLLIGLLSGS LAGLIDISAH WMTDLKEGIC TGGFWFNHEH
110 120 130 140 150
CCWNSEHVTF EDRDKCPEWN SWSQLIISTD QGAFAYIVNY FMYVLWALLF
160 170 180 190 200
AFLAVSLVKA FAPYACGSGI PEIKTILSGF IIRGYLGKWT LVIKTITLVL
210 220 230 240 250
AVSSGLSLGK EGPLVHVACC CGNILCHCFN KYRKNEAKRR EVLSAAAAAG
260 270 280 290 300
VSVAFGAPIG GVLFSLEEVS YYFPLKTLWR SFFAALVAAF TLRSINPFGN
310 320 330 340 350
SRLVLFYVEF HTPWHLFELV PFIVLGIFGG LWGALFIRTN IAWCRKRKTT
360 370 380 390 400
QLGKYPVVEV LIVTAITAIL AFPNEYTRMS TSELISELFN DCGLLDSSKL
410 420 430 440 450
CDYENHFNTS KGGELPDRPA GVGVYSAMWQ LALTLILKIV ITIFTFGMKI
460 470 480 490 500
PSGLFIPSMA VGAIAGRLLG VGMEQLAYYH HDWGIFNSWC SQGADCITPG
510 520 530 540 550
LYAMVGAAAC LGGVTRMTVS LVVIMFELTG GLEYIVPLMA AAMTSKWVAD
560 570 580 590 600
ALGREGIYDA HIRLNGYPFL EAKEEFAHKT LAMDVMKPRR NDPLLTVLTQ
610 620 630 640 650
DSMTVEDVET IISETTYSGF PVVVSRESQR LVGFVLRRDL IISIENARKK
660 670 680 690 700
QDGVVSTSII YFTEHSPPMP PYTPPTLKLR NILDLSPFTV TDLTPMEIVV
710 720 730 740
DIFRKLGLRQ CLVTHNGRLL GIITKKDVLK HIAQMANQDP ESILFN
Length:746
Mass (Da):83,068
Last modified:October 1, 1996 - v1
Checksum:i5F17D45F397003CE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti315H → Y in BAA09091 (PubMed:8626585).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z56277 mRNA. Translation: CAA91216.1.
D50497 mRNA. Translation: BAA09091.1.
RefSeqiNP_058802.1. NM_017106.1.
UniGeneiRn.10337.

Genome annotation databases

EnsembliENSRNOT00000003895; ENSRNOP00000003895; ENSRNOG00000002862.
GeneIDi25749.
KEGGirno:25749.
UCSCiRGD:2362. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z56277 mRNA. Translation: CAA91216.1.
D50497 mRNA. Translation: BAA09091.1.
RefSeqiNP_058802.1. NM_017106.1.
UniGeneiRn.10337.

3D structure databases

ProteinModelPortaliP51796.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003895.

PTM databases

PhosphoSitePlusiP51796.

Proteomic databases

PaxDbiP51796.
PRIDEiP51796.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000003895; ENSRNOP00000003895; ENSRNOG00000002862.
GeneIDi25749.
KEGGirno:25749.
UCSCiRGD:2362. rat.

Organism-specific databases

CTDi1184.
RGDi2362. Clcn5.

Phylogenomic databases

eggNOGiKOG0475. Eukaryota.
COG0038. LUCA.
GeneTreeiENSGT00760000119109.
HOGENOMiHOG000164493.
HOVERGENiHBG050984.
InParanoidiP51796.
KOiK05012.
PhylomeDBiP51796.

Enzyme and pathway databases

ReactomeiR-RNO-2672351. Stimuli-sensing channels.

Miscellaneous databases

PROiP51796.

Gene expression databases

BgeeiENSRNOG00000002862.
ExpressionAtlasiP51796. differential.
GenevisibleiP51796. RN.

Family and domain databases

Gene3Di1.10.3080.10. 3 hits.
InterProiIPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
IPR002247. Cl_channel-5.
[Graphical view]
PfamiPF00571. CBS. 2 hits.
PF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
PR01116. CLCHANNEL5.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
SUPFAMiSSF81340. SSF81340. 3 hits.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLCN5_RAT
AccessioniPrimary (citable) accession number: P51796
Secondary accession number(s): P70642
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons. The absence of conserved gating glutamate residues is typical for family members that function as channels (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.