P51785 (ILVD_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Alternative name(s):
Vegetative protein 110
Short name=VEG110

Gene names

Name:	ilvD
Ordered Locus Names:	BSU21870

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	558 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4
	Chain	2 – 558	557	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_0000103431
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	195	1	Iron-sulfur (4Fe-4S) Potential
Experimental info
	Sequence conflict	155	1	D → Y in AAA96629. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P51785 [UniParc]. Last modified July 7, 2009. Version 4. Checksum: CFFFF203CD555E61	FASTA	558	59,500
	10 20 30 40 50 60 MAELRSNMIT QGIDRAPHRS LLRAAGVKEE DFGKPFIAVC NSYIDIVPGH VHLQEFGKIV 70 80 90 100 110 120 KEAIREAGGV PFEFNTIGVD DGIAMGHIGM RYSLPSREII ADSVETVVSA HWFDGMVCIP 130 140 150 160 170 180 NCDKITPGML MAAMRINIPT IFVSGGPMAA GRTSDGRKIS LSSVFEGVGA YQAGKINENE 190 200 210 220 230 240 LQELEQFGCP TCGSCSGMFT ANSMNCLSEA LGLALPGNGT ILATSPERKE FVRKSAAQLM 250 260 270 280 290 300 ETIRKDIKPR DIVTVKAIDN AFALDMALGG STNTVLHTLA LANEAGVEYS LERINEVAER 310 320 330 340 350 360 VPHLAKLAPA SDVFIEDLHE AGGVSAALNE LSKKEGALHL DALTVTGKTL GETIAGHEVK 370 380 390 400 410 420 DYDVIHPLDQ PFTEKGGLAV LFGNLAPDGA IIKTGGVQNG ITRHEGPAVV FDSQDEALDG 430 440 450 460 470 480 IINRKVKEGD VVIIRYEGPK GGPGMPEMLA PTSQIVGMGL GPKVALITDG RFSGASRGLS 490 500 510 520 530 540 IGHVSPEAAE GGPLAFVENG DHIIVDIEKR ILDVQVPEEE WEKRKANWKG FEPKVKTGYL 550 ARYSKLVTSA NTGGIMKI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Organization of the Bacillus subtilis 168 chromosome between kdg and the attachment site of the SP beta prophage: use of long accurate PCR and yeast artificial chromosomes for sequencing." Capuano V., Galleron N., Pujic P., Sorokin A., Ehrlich S.D. Microbiology 142:3005-3015(1996) [PubMed: 8969496] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract] Cited for: SEQUENCE REVISION TO 155.
[4]	"First steps from a two-dimensional protein index towards a response-regulation map for Bacillus subtilis." Antelmann H., Bernhardt J., Schmid R., Mach H., Voelker U., Hecker M. Electrophoresis 18:1451-1463(1997) [PubMed: 9298659] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-16. Strain: 168 / IS58.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77246 Genomic DNA. Translation: AAA96629.1. AL009126 Genomic DNA. Translation: CAB14105.2.
PIR	D69644.
RefSeq	NP_390070.2. NC_000964.3.
3D structure databases
ProteinModelPortal	P51785.
SMR	P51785. Positions 65-557.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000000452; EBBACP00000000452; EBBACG00000000450.
GeneID	939084.
GenomeReviews	Gene locus BSU21870 in contig AL009126_GR.
KEGG	bsu:BSU21870.
NMPDR	fig|224308.1.peg.2193.
PATRIC	18976179. VBIBacSub10457_2280.
Organism-specific databases
GenoList	BSU21870. [Micado]
Phylogenomic databases
GeneTree	EBGT00050000001430.
HOGENOM	HBG671001.
PhylomeDB	P51785.
ProtClustDB	PRK00911.
Enzyme and pathway databases
BioCyc	BSUB:BSU21870-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_BACSU

Accession

Primary (citable) accession number: P51785

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	July 7, 2009
Last modified:	January 25, 2012
This is version 92 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program