Reviewed, UniProtKB/Swiss-Prot P51782 (LYSC_TRIVU)

Names and origin

Protein names

Recommended name:
    Lysozyme C
    EC=3.2.1.17
Alternative name(s):
    1,4-beta-N-acetylmuramidase C

Gene names

Name:

LYZ

Organism

Trichosurus vulpecula (Brush-tailed possum)

Taxonomic identifier

9337 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Metatheria › Diprotodontia › Phalangeridae › Trichosurus

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
Catalytic activity	Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Subunit structure	Monomer By similarity.
Miscellaneous	Lysozyme C is capable of both hydrolysis and transglycosylation; it shows also a slight esterase activity. It acts rapidly on both peptide-substituted and unsubstituted peptidoglycan, and slowly on chitin oligosaccharides.
Sequence similarities	Belongs to the glycosyl hydrolase 22 family.
Mass spectrometry	Molecular mass is 14896 Da from positions 19 - 147. Determined by MALDI. Ref.1

Ontologies

Keywords
Domain	Signal
Molecular function	Antimicrobial Bacteriolytic enzyme Glycosidase Hydrolase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	lysozyme activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18
	Chain	19 – 147	129	Lysozyme C		PRO_0000018493
Sites
	Active site	53	1	By similarity
	Active site	71	1	By similarity
Amino acid modifications
	Disulfide bond	24 ↔ 145		By similarity
	Disulfide bond	48 ↔ 133		By similarity
	Disulfide bond	83 ↔ 99		By similarity
	Disulfide bond	95 ↔ 113		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P51782-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: F281667A17F483AC	FASTA	147	16,839
	10 20 30 40 50 60 MKVLLLLGFI FCSMAAHGKR MERCEFARRI KQLHLDGYHQ ISLANWVCLA QWESGFDTKA 70 80 90 100 110 120 TNYNPGDQST DYGILQINSH YWCDDGKTPH AANECKVRCS ELQEDDLVKA VNCAKKIVDQ 130 140 QGIRAWVAWR NKCEGKDLSK YLEGCHL

References

[1]

"Lysozyme and alpha-lactalbumin from the milk of a marsupial, the common brush-tailed possum (Trichosurus vulpecula)." Piotte C.P., Marshall C.J., Hubbard M.J., Collet C., Grigor M.R. Biochim. Biophys. Acta 1336:235-242(1997) [PubMed: 9305795] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, MASS SPECTROMETRY. Tissue: Mammary gland.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U40664 Genomic DNA. Translation: AAB97109.1.
3D structure databases
HSSP	HSSP built from PDB template 1LZ6 based on UniProtKB P00695.
ModBase	Search...
Protein family/group databases
CAZy	GH22. Glycoside Hydrolase Family 22.
Phylogenomic databases
HOVERGEN	P51782.
Enzyme and pathway databases
BRENDA	3.2.1.17. 270554.
Family and domain databases
InterPro	IPR001916. Glyco_hydro_22. IPR019799. Glyco_hydro_22_CS. IPR000974. Glyco_hydro_22_lys. [Graphical view]
Pfam	PF00062. Lys. 1 hit. [Graphical view]
PRINTS	PR00137. LYSOZYME. PR00135. LYZLACT.
SMART	SM00263. LYZ1. 1 hit. [Graphical view]
PROSITE	PS00128. LACTALBUMIN_LYSOZYME_1. 1 hit. PS51348. LACTALBUMIN_LYSOZYME_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LYSC_TRIVU

Accession

Primary (citable) accession number: P51782

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)