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P51771

- ENLYS_BPP2

UniProt

P51771 - ENLYS_BPP2

Protein

Endolysin

Gene

K

Organism
Enterobacteria phage P2 (Bacteriophage P2)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
  1. Functioni

    Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer By similarity.By similarity

    Catalytic activityi

    Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei21 – 211By similarity

    GO - Molecular functioni

    1. lysozyme activity Source: UniProtKB-EC

    GO - Biological processi

    1. cell wall macromolecule catabolic process Source: InterPro
    2. cytolysis Source: UniProtKB-KW
    3. defense response to bacterium Source: UniProtKB-KW
    4. peptidoglycan catabolic process Source: InterPro
    5. viral release from host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    Antimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase

    Keywords - Biological processi

    Cytolysis, Host cell lysis by virus, Virus exit from host cell

    Protein family/group databases

    CAZyiGH104. Glycoside Hydrolase Family 104.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endolysin (EC:3.2.1.17)
    Alternative name(s):
    Lysis protein
    Lysozyme
    Muramidase
    Protein gpK
    Gene namesi
    Name:K
    OrganismiEnterobacteria phage P2 (Bacteriophage P2)
    Taxonomic identifieri10679 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaePeduovirinaeP2likevirus
    Virus hostiEnterobacteriaceae [TaxID: 543]
    ProteomesiUP000009092: Genome

    Subcellular locationi

    Host cytoplasm Curated
    Note: The endolysin is cytoplasmic, but can reach the periplasmic space with the help of the holins which disrupt the host cell membrane.By similarity

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 165165EndolysinPRO_0000218100Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP51771.
    SMRiP51771. Positions 7-164.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 24 family.Curated

    Family and domain databases

    InterProiIPR002196. Glyco_hydro_24.
    IPR023346. Lysozyme-like_dom.
    [Graphical view]
    PfamiPF00959. Phage_lysozyme. 1 hit.
    [Graphical view]
    SUPFAMiSSF53955. SSF53955. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P51771-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPVINTHQNI AAFLDMLAVS EGTANHPLTK NRGYDVIVTG LDGKPEIFTD    50
    YSDHPFAHGR PAKVFNRRGE KSTASGRYQQ LYLFWPHYRK QLALPDFSPL 100
    SQDRLAIQLI RERGALDDIR AGRIERAISR CRNIWASLPG AGYGQREHSL 150
    EKLVTVWRTA GGVPA 165
    Length:165
    Mass (Da):18,538
    Last modified:October 1, 1996 - v1
    Checksum:i82388A6A86C2B35E
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti40 – 401G → R in temperature-sensitive KTS60.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF063097 Genomic DNA. Translation: AAD03276.1.
    PIRiD55855.
    RefSeqiNP_046765.1. NC_001895.1.

    Genome annotation databases

    GeneIDi1261544.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF063097 Genomic DNA. Translation: AAD03276.1 .
    PIRi D55855.
    RefSeqi NP_046765.1. NC_001895.1.

    3D structure databases

    ProteinModelPortali P51771.
    SMRi P51771. Positions 7-164.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH104. Glycoside Hydrolase Family 104.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1261544.

    Family and domain databases

    InterProi IPR002196. Glyco_hydro_24.
    IPR023346. Lysozyme-like_dom.
    [Graphical view ]
    Pfami PF00959. Phage_lysozyme. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53955. SSF53955. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Functions involved in bacteriophage P2-induced host cell lysis and identification of a new tail gene."
      Ziermann R., Bartlett B., Calendar R., Christie G.E.
      J. Bacteriol. 176:4974-4984(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiENLYS_BPP2
    AccessioniPrimary (citable) accession number: P51771
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3