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Protein

Endolysin

Gene

K

Organism
Escherichia phage P2 (Bacteriophage P2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Endolysin with transglycosylase activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.UniRule annotation

Catalytic activityi

Endolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei21UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAntimicrobial, Bacteriolytic enzyme, Lyase
Biological processCytolysis, Host cell lysis by virus, Virus exit from host cell

Protein family/group databases

CAZyiGH104. Glycoside Hydrolase Family 104.

Names & Taxonomyi

Protein namesi
Recommended name:
EndolysinUniRule annotation (EC:4.2.2.n2UniRule annotation)
Alternative name(s):
Lysis proteinUniRule annotation
LysozymeUniRule annotation
Protein gpKCurated
TransglycosylaseUniRule annotation
Gene namesi
Name:K
OrganismiEscherichia phage P2 (Bacteriophage P2)
Taxonomic identifieri10679 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaePeduovirinaeP2virus
Virus hostiEnterobacteriaceae [TaxID: 543]
Proteomesi
  • UP000009092 Componenti: Genome

Subcellular locationi

  • Host cytoplasm UniRule annotation

  • Note: The endolysin is cytoplasmic, but can reach the periplasmic space with the help of the holins which disrupt the host cell membrane.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002181001 – 165EndolysinAdd BLAST165

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP51771.
SMRiP51771.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 24 family.UniRule annotation

Phylogenomic databases

OrthoDBiVOG090000W9.

Family and domain databases

HAMAPiMF_04109. ENDOLYSIN_LAMBDA. 1 hit.
InterProiView protein in InterPro
IPR034691. Endolysin_lambda_type.
IPR002196. Glyco_hydro_24.
IPR023346. Lysozyme-like_dom.
PfamiView protein in Pfam
PF00959. Phage_lysozyme. 1 hit.
SUPFAMiSSF53955. SSF53955. 1 hit.

Sequencei

Sequence statusi: Complete.

P51771-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVINTHQNI AAFLDMLAVS EGTANHPLTK NRGYDVIVTG LDGKPEIFTD
60 70 80 90 100
YSDHPFAHGR PAKVFNRRGE KSTASGRYQQ LYLFWPHYRK QLALPDFSPL
110 120 130 140 150
SQDRLAIQLI RERGALDDIR AGRIERAISR CRNIWASLPG AGYGQREHSL
160
EKLVTVWRTA GGVPA
Length:165
Mass (Da):18,538
Last modified:October 1, 1996 - v1
Checksum:i82388A6A86C2B35E
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti40G → R in temperature-sensitive KTS60. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF063097 Genomic DNA. Translation: AAD03276.1.
PIRiD55855.
RefSeqiNP_046765.1. NC_001895.1.

Genome annotation databases

GeneIDi1261544.
KEGGivg:1261544.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF063097 Genomic DNA. Translation: AAD03276.1.
PIRiD55855.
RefSeqiNP_046765.1. NC_001895.1.

3D structure databases

ProteinModelPortaliP51771.
SMRiP51771.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH104. Glycoside Hydrolase Family 104.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1261544.
KEGGivg:1261544.

Phylogenomic databases

OrthoDBiVOG090000W9.

Family and domain databases

HAMAPiMF_04109. ENDOLYSIN_LAMBDA. 1 hit.
InterProiView protein in InterPro
IPR034691. Endolysin_lambda_type.
IPR002196. Glyco_hydro_24.
IPR023346. Lysozyme-like_dom.
PfamiView protein in Pfam
PF00959. Phage_lysozyme. 1 hit.
SUPFAMiSSF53955. SSF53955. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiENLYS_BPP2
AccessioniPrimary (citable) accession number: P51771
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 12, 2017
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.