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P51740

- PPBI2_RAT

UniProt

P51740 - PPBI2_RAT

Protein

Intestinal-type alkaline phosphatase 2

Gene
N/A
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

    Cofactori

    Binds 1 magnesium ion.By similarity
    Binds 2 zinc ions.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi61 – 611MagnesiumBy similarity
    Metal bindingi61 – 611Zinc 1By similarity
    Active sitei111 – 1111Phosphoserine intermediatePROSITE-ProRule annotation
    Metal bindingi111 – 1111Zinc 1By similarity
    Metal bindingi174 – 1741MagnesiumBy similarity
    Metal bindingi330 – 3301MagnesiumBy similarity
    Metal bindingi335 – 3351Zinc 2By similarity
    Metal bindingi339 – 3391Zinc 2; via tele nitrogenBy similarity
    Metal bindingi376 – 3761Zinc 1By similarity
    Metal bindingi377 – 3771Zinc 1; via tele nitrogenBy similarity

    GO - Molecular functioni

    1. alkaline phosphatase activity Source: RGD
    2. magnesium ion binding Source: RGD
    3. zinc ion binding Source: RGD

    GO - Biological processi

    1. dephosphorylation Source: GOC
    2. phosphorus metabolic process Source: RGD

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Intestinal-type alkaline phosphatase 2 (EC:3.1.3.1)
    Short name:
    IAP-2
    Short name:
    Intestinal alkaline phosphatase 2
    Alternative name(s):
    Intestinal alkaline phosphatase II
    Short name:
    IAP-II
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. external side of plasma membrane Source: RGD
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 19191 PublicationAdd
    BLAST
    Chaini20 – 531512Intestinal-type alkaline phosphatase 2PRO_0000024043Add
    BLAST
    Propeptidei532 – 55120Removed in mature formSequence AnalysisPRO_0000024044Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi140 ↔ 202By similarity
    Glycosylationi141 – 1411N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi241 – 2411N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi426 – 4261N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi485 ↔ 492By similarity
    Glycosylationi509 – 5091N-linked (GlcNAc...)Sequence Analysis
    Lipidationi531 – 5311GPI-anchor amidated asparagineSequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiP51740.
    PRIDEiP51740.

    Expressioni

    Gene expression databases

    GenevestigatoriP51740.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000026294.

    Structurei

    3D structure databases

    ProteinModelPortaliP51740.
    SMRiP51740. Positions 20-498.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi511 – 52717Poly-ThrAdd
    BLAST

    Sequence similaritiesi

    Belongs to the alkaline phosphatase family.Curated

    Keywords - Domaini

    Signal, Transmembrane

    Phylogenomic databases

    eggNOGiCOG1785.
    HOVERGENiHBG007345.
    PhylomeDBiP51740.

    Family and domain databases

    Gene3Di3.40.720.10. 1 hit.
    InterProiIPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view]
    PfamiPF00245. Alk_phosphatase. 1 hit.
    [Graphical view]
    PRINTSiPR00113. ALKPHPHTASE.
    SMARTiSM00098. alkPPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF53649. SSF53649. 1 hit.
    PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P51740-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQGAWVLLLL GFRLQLSLSV IPVEEENPAF WTQKAADALN VAKKLQPIQT    50
    SAKNLIIFLG DGMGVATVTA TRILKGQLEG NLGPETPLAM DHFPYMALSK 100
    TYSVDRQVPD SASTATAYLC GVKTNYKTIG VSAAARFDQC NTTFGNEVLS 150
    VMYRAKKAGK SVGVGDHTRV QHASPAGTYV HTVTSNWYGD ADMPALPLQE 200
    GCKDIATQLI SNMDINVILG GGRKYMFPAG TPDPEYPNDV NETGTRLDGK 250
    NLVQEWLSKH QGSQYVWNRQ ELIQKSLDPS VTYLMGLFEP VDTKFEIQRD 300
    PLMDPSLKDM TEAALHVLSR NPKGFYLFVE GGRIDRGHHL GTAYLALTEA 350
    VMFDSAIERA SLQASEQDTL TIVTADHSHV FSFGGYTLRG TSIFGLAPLN 400
    ALDGKPYTSI LYGNGPGYVG TGERPNVTDA ESHDPSYQQQ AAVPVKSETT 450
    VGKDVAIFAR GPQAHLLHGV QEQNYIAHVM AFAGCLEPYT DCGLAPPADE 500
    NRPTTPVQNS TTTTTTTTTT TTTTTTTRVQ NSASSLGPAT APLAWHYWPR 550
    R 551
    Length:551
    Mass (Da):59,797
    Last modified:October 1, 1996 - v1
    Checksum:i486206A5A9A11C3B
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti29 – 291A → V.1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S66545 mRNA. Translation: AAB20378.2.
    PIRiB56888.
    S18408.
    UniGeneiRn.138122.

    Genome annotation databases

    UCSCiRGD:621650. rat.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S66545 mRNA. Translation: AAB20378.2 .
    PIRi B56888.
    S18408.
    UniGenei Rn.138122.

    3D structure databases

    ProteinModelPortali P51740.
    SMRi P51740. Positions 20-498.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000026294.

    Proteomic databases

    PaxDbi P51740.
    PRIDEi P51740.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:621650. rat.

    Phylogenomic databases

    eggNOGi COG1785.
    HOVERGENi HBG007345.
    PhylomeDBi P51740.

    Gene expression databases

    Genevestigatori P51740.

    Family and domain databases

    Gene3Di 3.40.720.10. 1 hit.
    InterProi IPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view ]
    Pfami PF00245. Alk_phosphatase. 1 hit.
    [Graphical view ]
    PRINTSi PR00113. ALKPHPHTASE.
    SMARTi SM00098. alkPPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53649. SSF53649. 1 hit.
    PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of a mRNA that encodes a putative intestinal alkaline phosphatase regulated by 1,25-dihydroxyvitamin D-3."
      Strom M., Krisinger J., Deluca H.F.
      Biochim. Biophys. Acta 1090:299-304(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The two mRNAs encoding rat intestinal alkaline phosphatase represent two distinct nucleotide sequences."
      Engle M.J., Aleprs D.H.
      Clin. Chem. 38:2506-2509(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL NUCLEOTIDE SEQUENCE.
      Strain: Sprague-Dawley.
    3. "Purification and characterization of phytase from rat intestinal mucosa."
      Yang W.-J., Matsuda Y., Sano S., Masutani H., Nakagawa H.
      Biochim. Biophys. Acta 1075:75-82(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 20-29, VARIANT VAL-29.
    4. "Two rat intestinal alkaline phosphatase isoforms with different carboxyl-terminal peptides are both membrane-bound by a glycan phosphatidylinositol linkage."
      Engle M.J., Mahmood A., Alpers D.H.
      J. Biol. Chem. 270:11935-11940(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: GPI-ANCHOR.

    Entry informationi

    Entry nameiPPBI2_RAT
    AccessioniPrimary (citable) accession number: P51740
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney). Rat has two genes for the intestinal isozyme.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3