Intestinal-type alkaline phosphatase 2 precursor

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P51740 (PPBI2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 96. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names	Recommended name: Intestinal-type alkaline phosphatase 2 Short name=IAP-2 Short name=Intestinal alkaline phosphatase 2 EC=3.1.3.1 Alternative name(s): Intestinal alkaline phosphatase II Short name=IAP-II
Organism	Rattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier	10116 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	551 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	A phosphate monoester + H₂O = an alcohol + phosphate.
Cofactor	Binds 1 magnesium ion By similarity. Binds 2 zinc ions By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor Ref.4.
Miscellaneous	In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney). Rat has two genes for the intestinal isozyme.
Sequence similarities	Belongs to the alkaline phosphatase family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Signal Transmembrane
Ligand	Magnesium Metal-binding Zinc
Molecular function	Hydrolase
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW external side of plasma membrane Inferred from direct assay Ref.4. Source: RGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	alkaline phosphatase activity Inferred from direct assay PubMed 15885097. Source: RGD magnesium ion binding Inferred from direct assay PubMed 15885097. Source: RGD zinc ion binding Inferred from direct assay PubMed 15885097. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

Ref.3

Chain

20 – 531

512

Intestinal-type alkaline phosphatase 2

PRO_0000024043

Propeptide

532 – 551

Removed in mature form Potential

PRO_0000024044

Regions

Compositional bias

511 – 527

Poly-Thr

Sites

Active site

111

Phosphoserine intermediate By similarity

Metal binding

Magnesium Potential

Metal binding

Zinc 2 Potential

Metal binding

330

Magnesium Potential

Metal binding

335

Zinc 1 Potential

Metal binding

339

Zinc 1 Potential

Metal binding

376

Zinc 2 Potential

Metal binding

377

Zinc 2 Potential

Amino acid modifications

Lipidation

531

GPI-anchor amidated asparagine Potential

Glycosylation

141

N-linked (GlcNAc...) Potential

Glycosylation

241

N-linked (GlcNAc...) Potential

Glycosylation

426

N-linked (GlcNAc...) Potential

Glycosylation

509

N-linked (GlcNAc...) Potential

Disulfide bond

140 ↔ 202

By similarity

Disulfide bond

485 ↔ 492

By similarity

Natural variations

Natural variant

A → V. Ref.3

Sequences

Sequence

Length

Mass (Da)

Tools

P51740 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 486206A5A9A11C3B
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FASTA

551

59,797

        10         20         30         40         50         60 
MQGAWVLLLL GFRLQLSLSV IPVEEENPAF WTQKAADALN VAKKLQPIQT SAKNLIIFLG 

        70         80         90        100        110        120 
DGMGVATVTA TRILKGQLEG NLGPETPLAM DHFPYMALSK TYSVDRQVPD SASTATAYLC 

       130        140        150        160        170        180 
GVKTNYKTIG VSAAARFDQC NTTFGNEVLS VMYRAKKAGK SVGVGDHTRV QHASPAGTYV 

       190        200        210        220        230        240 
HTVTSNWYGD ADMPALPLQE GCKDIATQLI SNMDINVILG GGRKYMFPAG TPDPEYPNDV 

       250        260        270        280        290        300 
NETGTRLDGK NLVQEWLSKH QGSQYVWNRQ ELIQKSLDPS VTYLMGLFEP VDTKFEIQRD 

       310        320        330        340        350        360 
PLMDPSLKDM TEAALHVLSR NPKGFYLFVE GGRIDRGHHL GTAYLALTEA VMFDSAIERA 

       370        380        390        400        410        420 
SLQASEQDTL TIVTADHSHV FSFGGYTLRG TSIFGLAPLN ALDGKPYTSI LYGNGPGYVG 

       430        440        450        460        470        480 
TGERPNVTDA ESHDPSYQQQ AAVPVKSETT VGKDVAIFAR GPQAHLLHGV QEQNYIAHVM 

       490        500        510        520        530        540 
AFAGCLEPYT DCGLAPPADE NRPTTPVQNS TTTTTTTTTT TTTTTTTRVQ NSASSLGPAT 

       550 
APLAWHYWPR R

« Hide

References

[1]	"Isolation of a mRNA that encodes a putative intestinal alkaline phosphatase regulated by 1,25-dihydroxyvitamin D-3." Strom M., Krisinger J., Deluca H.F. Biochim. Biophys. Acta 1090:299-304(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The two mRNAs encoding rat intestinal alkaline phosphatase represent two distinct nucleotide sequences." Engle M.J., Aleprs D.H. Clin. Chem. 38:2506-2509(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE. Strain: Sprague-Dawley.
[3]	"Purification and characterization of phytase from rat intestinal mucosa." Yang W.-J., Matsuda Y., Sano S., Masutani H., Nakagawa H. Biochim. Biophys. Acta 1075:75-82(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-29, VARIANT VAL-29.
[4]	"Two rat intestinal alkaline phosphatase isoforms with different carboxyl-terminal peptides are both membrane-bound by a glycan phosphatidylinositol linkage." Engle M.J., Mahmood A., Alpers D.H. J. Biol. Chem. 270:11935-11940(1995) [PubMed] [Europe PMC] [Abstract] Cited for: GPI-ANCHOR.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S66545 mRNA. Translation: AAB20378.2.
IPI	IPI00205280.
PIR	B56888. S18408.
UniGene	Rn.138122.
3D structure databases
ProteinModelPortal	P51740.
SMR	P51740. Positions 20-498.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000026294.
Proteomic databases
PaxDb	P51740.
PRIDE	P51740.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
UCSC	RGD:621650. rat.
Phylogenomic databases
eggNOG	COG1785.
HOVERGEN	HBG007345.
OrthoDB	EOG4GB765.
Gene expression databases
Genevestigator	P51740.
GermOnline	ENSRNOG00000019375. Rattus norvegicus.
Family and domain databases
Gene3D	3.40.720.10. 1 hit.
InterPro	IPR017849. Alkaline_Pase-like_a/b/a. IPR001952. Alkaline_phosphatase. IPR018299. Alkaline_phosphatase_AS. IPR017850. Alkaline_phosphatase_core. [Graphical view]
Pfam	PF00245. Alk_phosphatase. 1 hit. [Graphical view]
PRINTS	PR00113. ALKPHPHTASE.
SMART	SM00098. alkPPc. 1 hit. [Graphical view]
SUPFAM	SSF53649. Alkaline_phosphatase_core. 1 hit.
PROSITE	PS00123. ALKALINE_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPBI2_RAT

Accession

Primary (citable) accession number: P51740

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents