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Protein

Intestinal-type alkaline phosphatase 2

Gene
N/A
Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney). Rat has two genes for the intestinal isozyme.

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ ion.By similarity
  • Zn2+By similarityNote: Binds 2 Zn2+ ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi61MagnesiumBy similarity1
Metal bindingi61Zinc 1By similarity1
Active sitei111Phosphoserine intermediatePROSITE-ProRule annotation1
Metal bindingi111Zinc 1By similarity1
Metal bindingi174MagnesiumBy similarity1
Metal bindingi330MagnesiumBy similarity1
Metal bindingi335Zinc 2By similarity1
Metal bindingi339Zinc 2; via tele nitrogenBy similarity1
Metal bindingi376Zinc 1By similarity1
Metal bindingi377Zinc 1; via tele nitrogenBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase
LigandMagnesium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Intestinal-type alkaline phosphatase 2 (EC:3.1.3.1)
Short name:
IAP-2
Short name:
Intestinal alkaline phosphatase 2
Alternative name(s):
Intestinal alkaline phosphatase II
Short name:
IAP-II
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 191 PublicationAdd BLAST19
ChainiPRO_000002404320 – 531Intestinal-type alkaline phosphatase 2Add BLAST512
PropeptideiPRO_0000024044532 – 551Removed in mature formSequence analysisAdd BLAST20

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi140 ↔ 202By similarity
Glycosylationi141N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi241N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi426N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi485 ↔ 492By similarity
Glycosylationi509N-linked (GlcNAc...) asparagineSequence analysis1
Lipidationi531GPI-anchor amidated asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiP51740.
PRIDEiP51740.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000066949.

Structurei

3D structure databases

ProteinModelPortaliP51740.
SMRiP51740.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi511 – 527Poly-ThrAdd BLAST17

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal, Transmembrane

Phylogenomic databases

eggNOGiKOG4126. Eukaryota.
COG1785. LUCA.
HOVERGENiHBG007345.
PhylomeDBiP51740.

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiView protein in InterPro
IPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
PANTHERiPTHR11596. PTHR11596. 1 hit.
PfamiView protein in Pfam
PF00245. Alk_phosphatase. 1 hit.
PRINTSiPR00113. ALKPHPHTASE.
SMARTiView protein in SMART
SM00098. alkPPc. 1 hit.
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiView protein in PROSITE
PS00123. ALKALINE_PHOSPHATASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P51740-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQGAWVLLLL GFRLQLSLSV IPVEEENPAF WTQKAADALN VAKKLQPIQT
60 70 80 90 100
SAKNLIIFLG DGMGVATVTA TRILKGQLEG NLGPETPLAM DHFPYMALSK
110 120 130 140 150
TYSVDRQVPD SASTATAYLC GVKTNYKTIG VSAAARFDQC NTTFGNEVLS
160 170 180 190 200
VMYRAKKAGK SVGVGDHTRV QHASPAGTYV HTVTSNWYGD ADMPALPLQE
210 220 230 240 250
GCKDIATQLI SNMDINVILG GGRKYMFPAG TPDPEYPNDV NETGTRLDGK
260 270 280 290 300
NLVQEWLSKH QGSQYVWNRQ ELIQKSLDPS VTYLMGLFEP VDTKFEIQRD
310 320 330 340 350
PLMDPSLKDM TEAALHVLSR NPKGFYLFVE GGRIDRGHHL GTAYLALTEA
360 370 380 390 400
VMFDSAIERA SLQASEQDTL TIVTADHSHV FSFGGYTLRG TSIFGLAPLN
410 420 430 440 450
ALDGKPYTSI LYGNGPGYVG TGERPNVTDA ESHDPSYQQQ AAVPVKSETT
460 470 480 490 500
VGKDVAIFAR GPQAHLLHGV QEQNYIAHVM AFAGCLEPYT DCGLAPPADE
510 520 530 540 550
NRPTTPVQNS TTTTTTTTTT TTTTTTTRVQ NSASSLGPAT APLAWHYWPR

R
Length:551
Mass (Da):59,797
Last modified:October 1, 1996 - v1
Checksum:i486206A5A9A11C3B
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti29A → V1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S66545 mRNA. Translation: AAB20378.2.
PIRiB56888.
S18408.
UniGeneiRn.138122.

Genome annotation databases

UCSCiRGD:621650. rat.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPPBI2_RAT
AccessioniPrimary (citable) accession number: P51740
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 10, 2017
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families