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Protein

Intestinal-type alkaline phosphatase 2

Gene
N/A
Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ ion.By similarity
  • Zn2+By similarityNote: Binds 2 Zn2+ ions.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi61 – 611MagnesiumBy similarity
Metal bindingi61 – 611Zinc 1By similarity
Active sitei111 – 1111Phosphoserine intermediatePROSITE-ProRule annotation
Metal bindingi111 – 1111Zinc 1By similarity
Metal bindingi174 – 1741MagnesiumBy similarity
Metal bindingi330 – 3301MagnesiumBy similarity
Metal bindingi335 – 3351Zinc 2By similarity
Metal bindingi339 – 3391Zinc 2; via tele nitrogenBy similarity
Metal bindingi376 – 3761Zinc 1By similarity
Metal bindingi377 – 3771Zinc 1; via tele nitrogenBy similarity

GO - Molecular functioni

  1. alkaline phosphatase activity Source: RGD
  2. magnesium ion binding Source: RGD
  3. zinc ion binding Source: RGD

GO - Biological processi

  1. dephosphorylation Source: GOC
  2. phosphorus metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

SABIO-RKP51740.

Names & Taxonomyi

Protein namesi
Recommended name:
Intestinal-type alkaline phosphatase 2 (EC:3.1.3.1)
Short name:
IAP-2
Short name:
Intestinal alkaline phosphatase 2
Alternative name(s):
Intestinal alkaline phosphatase II
Short name:
IAP-II
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. external side of plasma membrane Source: RGD
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 19191 PublicationAdd
BLAST
Chaini20 – 531512Intestinal-type alkaline phosphatase 2PRO_0000024043Add
BLAST
Propeptidei532 – 55120Removed in mature formSequence AnalysisPRO_0000024044Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi140 ↔ 202By similarity
Glycosylationi141 – 1411N-linked (GlcNAc...)Sequence Analysis
Glycosylationi241 – 2411N-linked (GlcNAc...)Sequence Analysis
Glycosylationi426 – 4261N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi485 ↔ 492By similarity
Glycosylationi509 – 5091N-linked (GlcNAc...)Sequence Analysis
Lipidationi531 – 5311GPI-anchor amidated asparagineSequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Proteomic databases

PaxDbiP51740.
PRIDEiP51740.

Expressioni

Gene expression databases

GenevestigatoriP51740.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026294.

Structurei

3D structure databases

ProteinModelPortaliP51740.
SMRiP51740. Positions 20-498.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi511 – 52717Poly-ThrAdd
BLAST

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal, Transmembrane

Phylogenomic databases

eggNOGiCOG1785.
HOVERGENiHBG007345.
PhylomeDBiP51740.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P51740-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQGAWVLLLL GFRLQLSLSV IPVEEENPAF WTQKAADALN VAKKLQPIQT
60 70 80 90 100
SAKNLIIFLG DGMGVATVTA TRILKGQLEG NLGPETPLAM DHFPYMALSK
110 120 130 140 150
TYSVDRQVPD SASTATAYLC GVKTNYKTIG VSAAARFDQC NTTFGNEVLS
160 170 180 190 200
VMYRAKKAGK SVGVGDHTRV QHASPAGTYV HTVTSNWYGD ADMPALPLQE
210 220 230 240 250
GCKDIATQLI SNMDINVILG GGRKYMFPAG TPDPEYPNDV NETGTRLDGK
260 270 280 290 300
NLVQEWLSKH QGSQYVWNRQ ELIQKSLDPS VTYLMGLFEP VDTKFEIQRD
310 320 330 340 350
PLMDPSLKDM TEAALHVLSR NPKGFYLFVE GGRIDRGHHL GTAYLALTEA
360 370 380 390 400
VMFDSAIERA SLQASEQDTL TIVTADHSHV FSFGGYTLRG TSIFGLAPLN
410 420 430 440 450
ALDGKPYTSI LYGNGPGYVG TGERPNVTDA ESHDPSYQQQ AAVPVKSETT
460 470 480 490 500
VGKDVAIFAR GPQAHLLHGV QEQNYIAHVM AFAGCLEPYT DCGLAPPADE
510 520 530 540 550
NRPTTPVQNS TTTTTTTTTT TTTTTTTRVQ NSASSLGPAT APLAWHYWPR

R
Length:551
Mass (Da):59,797
Last modified:October 1, 1996 - v1
Checksum:i486206A5A9A11C3B
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti29 – 291A → V.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S66545 mRNA. Translation: AAB20378.2.
PIRiB56888.
S18408.
UniGeneiRn.138122.

Genome annotation databases

UCSCiRGD:621650. rat.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S66545 mRNA. Translation: AAB20378.2.
PIRiB56888.
S18408.
UniGeneiRn.138122.

3D structure databases

ProteinModelPortaliP51740.
SMRiP51740. Positions 20-498.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026294.

Proteomic databases

PaxDbiP51740.
PRIDEiP51740.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:621650. rat.

Phylogenomic databases

eggNOGiCOG1785.
HOVERGENiHBG007345.
PhylomeDBiP51740.

Enzyme and pathway databases

SABIO-RKP51740.

Gene expression databases

GenevestigatoriP51740.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation of a mRNA that encodes a putative intestinal alkaline phosphatase regulated by 1,25-dihydroxyvitamin D-3."
    Strom M., Krisinger J., Deluca H.F.
    Biochim. Biophys. Acta 1090:299-304(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The two mRNAs encoding rat intestinal alkaline phosphatase represent two distinct nucleotide sequences."
    Engle M.J., Aleprs D.H.
    Clin. Chem. 38:2506-2509(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL NUCLEOTIDE SEQUENCE.
    Strain: Sprague-Dawley.
  3. "Purification and characterization of phytase from rat intestinal mucosa."
    Yang W.-J., Matsuda Y., Sano S., Masutani H., Nakagawa H.
    Biochim. Biophys. Acta 1075:75-82(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 20-29, VARIANT VAL-29.
  4. "Two rat intestinal alkaline phosphatase isoforms with different carboxyl-terminal peptides are both membrane-bound by a glycan phosphatidylinositol linkage."
    Engle M.J., Mahmood A., Alpers D.H.
    J. Biol. Chem. 270:11935-11940(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: GPI-ANCHOR.

Entry informationi

Entry nameiPPBI2_RAT
AccessioniPrimary (citable) accession number: P51740
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 7, 2015
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney). Rat has two genes for the intestinal isozyme.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.