Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P51740 (PPBI2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Intestinal-type alkaline phosphatase 2

Short name=IAP-2
Short name=Intestinal alkaline phosphatase 2
EC=3.1.3.1
Alternative name(s):
Intestinal alkaline phosphatase II
Short name=IAP-II
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length551 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactor

Binds 1 magnesium ion By similarity.

Binds 2 zinc ions By similarity.

Subunit structure

Homodimer By similarity.

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor Ref.4.

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney). Rat has two genes for the intestinal isozyme.

Sequence similarities

Belongs to the alkaline phosphatase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Ref.3
Chain20 – 531512Intestinal-type alkaline phosphatase 2
PRO_0000024043
Propeptide532 – 55120Removed in mature form Potential
PRO_0000024044

Regions

Compositional bias511 – 52717Poly-Thr

Sites

Active site1111Phosphoserine intermediate By similarity
Metal binding611Magnesium By similarity
Metal binding611Zinc 1 By similarity
Metal binding1111Zinc 1 By similarity
Metal binding1741Magnesium By similarity
Metal binding3301Magnesium By similarity
Metal binding3351Zinc 2 By similarity
Metal binding3391Zinc 2; via tele nitrogen By similarity
Metal binding3761Zinc 1 By similarity
Metal binding3771Zinc 1; via tele nitrogen By similarity

Amino acid modifications

Lipidation5311GPI-anchor amidated asparagine Potential
Glycosylation1411N-linked (GlcNAc...) Potential
Glycosylation2411N-linked (GlcNAc...) Potential
Glycosylation4261N-linked (GlcNAc...) Potential
Glycosylation5091N-linked (GlcNAc...) Potential
Disulfide bond140 ↔ 202 By similarity
Disulfide bond485 ↔ 492 By similarity

Natural variations

Natural variant291A → V. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P51740 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 486206A5A9A11C3B

FASTA55159,797
        10         20         30         40         50         60 
MQGAWVLLLL GFRLQLSLSV IPVEEENPAF WTQKAADALN VAKKLQPIQT SAKNLIIFLG 

        70         80         90        100        110        120 
DGMGVATVTA TRILKGQLEG NLGPETPLAM DHFPYMALSK TYSVDRQVPD SASTATAYLC 

       130        140        150        160        170        180 
GVKTNYKTIG VSAAARFDQC NTTFGNEVLS VMYRAKKAGK SVGVGDHTRV QHASPAGTYV 

       190        200        210        220        230        240 
HTVTSNWYGD ADMPALPLQE GCKDIATQLI SNMDINVILG GGRKYMFPAG TPDPEYPNDV 

       250        260        270        280        290        300 
NETGTRLDGK NLVQEWLSKH QGSQYVWNRQ ELIQKSLDPS VTYLMGLFEP VDTKFEIQRD 

       310        320        330        340        350        360 
PLMDPSLKDM TEAALHVLSR NPKGFYLFVE GGRIDRGHHL GTAYLALTEA VMFDSAIERA 

       370        380        390        400        410        420 
SLQASEQDTL TIVTADHSHV FSFGGYTLRG TSIFGLAPLN ALDGKPYTSI LYGNGPGYVG 

       430        440        450        460        470        480 
TGERPNVTDA ESHDPSYQQQ AAVPVKSETT VGKDVAIFAR GPQAHLLHGV QEQNYIAHVM 

       490        500        510        520        530        540 
AFAGCLEPYT DCGLAPPADE NRPTTPVQNS TTTTTTTTTT TTTTTTTRVQ NSASSLGPAT 

       550 
APLAWHYWPR R 

« Hide

References

[1]"Isolation of a mRNA that encodes a putative intestinal alkaline phosphatase regulated by 1,25-dihydroxyvitamin D-3."
Strom M., Krisinger J., Deluca H.F.
Biochim. Biophys. Acta 1090:299-304(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The two mRNAs encoding rat intestinal alkaline phosphatase represent two distinct nucleotide sequences."
Engle M.J., Aleprs D.H.
Clin. Chem. 38:2506-2509(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL NUCLEOTIDE SEQUENCE.
Strain: Sprague-Dawley.
[3]"Purification and characterization of phytase from rat intestinal mucosa."
Yang W.-J., Matsuda Y., Sano S., Masutani H., Nakagawa H.
Biochim. Biophys. Acta 1075:75-82(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 20-29, VARIANT VAL-29.
[4]"Two rat intestinal alkaline phosphatase isoforms with different carboxyl-terminal peptides are both membrane-bound by a glycan phosphatidylinositol linkage."
Engle M.J., Mahmood A., Alpers D.H.
J. Biol. Chem. 270:11935-11940(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: GPI-ANCHOR.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S66545 mRNA. Translation: AAB20378.2.
PIRB56888.
S18408.
UniGeneRn.138122.

3D structure databases

ProteinModelPortalP51740.
SMRP51740. Positions 20-498.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000026294.

Proteomic databases

PaxDbP51740.
PRIDEP51740.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:621650. rat.

Phylogenomic databases

eggNOGCOG1785.
HOVERGENHBG007345.
PhylomeDBP51740.

Enzyme and pathway databases

SABIO-RKP51740.

Gene expression databases

GenevestigatorP51740.

Family and domain databases

Gene3D3.40.720.10. 1 hit.
InterProIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSPR00113. ALKPHPHTASE.
SMARTSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMSSF53649. SSF53649. 1 hit.
PROSITEPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePPBI2_RAT
AccessionPrimary (citable) accession number: P51740
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families