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P51740

- PPBI2_RAT

UniProt

P51740 - PPBI2_RAT

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Protein
Intestinal-type alkaline phosphatase 2
Gene
N/A
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Binds 1 magnesium ion By similarity.
Binds 2 zinc ions By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi61 – 611Magnesium By similarity
Metal bindingi61 – 611Zinc 1 By similarity
Active sitei111 – 1111Phosphoserine intermediate By similarity
Metal bindingi111 – 1111Zinc 1 By similarity
Metal bindingi174 – 1741Magnesium By similarity
Metal bindingi330 – 3301Magnesium By similarity
Metal bindingi335 – 3351Zinc 2 By similarity
Metal bindingi339 – 3391Zinc 2; via tele nitrogen By similarity
Metal bindingi376 – 3761Zinc 1 By similarity
Metal bindingi377 – 3771Zinc 1; via tele nitrogen By similarity

GO - Molecular functioni

  1. alkaline phosphatase activity Source: RGD
  2. magnesium ion binding Source: RGD
  3. zinc ion binding Source: RGD

GO - Biological processi

  1. dephosphorylation Source: GOC
  2. phosphorus metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Intestinal-type alkaline phosphatase 2 (EC:3.1.3.1)
Short name:
IAP-2
Short name:
Intestinal alkaline phosphatase 2
Alternative name(s):
Intestinal alkaline phosphatase II
Short name:
IAP-II
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Subcellular locationi

Cell membrane; Lipid-anchorGPI-anchor 1 Publication

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. external side of plasma membrane Source: RGD
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 19191 Publication
Add
BLAST
Chaini20 – 531512Intestinal-type alkaline phosphatase 2
PRO_0000024043Add
BLAST
Propeptidei532 – 55120Removed in mature form Reviewed prediction
PRO_0000024044Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi140 ↔ 202 By similarity
Glycosylationi141 – 1411N-linked (GlcNAc...) Reviewed prediction
Glycosylationi241 – 2411N-linked (GlcNAc...) Reviewed prediction
Glycosylationi426 – 4261N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi485 ↔ 492 By similarity
Glycosylationi509 – 5091N-linked (GlcNAc...) Reviewed prediction
Lipidationi531 – 5311GPI-anchor amidated asparagine Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Proteomic databases

PaxDbiP51740.
PRIDEiP51740.

Expressioni

Gene expression databases

GenevestigatoriP51740.

Interactioni

Subunit structurei

Homodimer By similarity.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026294.

Structurei

3D structure databases

ProteinModelPortaliP51740.
SMRiP51740. Positions 20-498.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi511 – 52717Poly-Thr
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane

Phylogenomic databases

eggNOGiCOG1785.
HOVERGENiHBG007345.
PhylomeDBiP51740.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P51740-1 [UniParc]FASTAAdd to Basket

« Hide

MQGAWVLLLL GFRLQLSLSV IPVEEENPAF WTQKAADALN VAKKLQPIQT    50
SAKNLIIFLG DGMGVATVTA TRILKGQLEG NLGPETPLAM DHFPYMALSK 100
TYSVDRQVPD SASTATAYLC GVKTNYKTIG VSAAARFDQC NTTFGNEVLS 150
VMYRAKKAGK SVGVGDHTRV QHASPAGTYV HTVTSNWYGD ADMPALPLQE 200
GCKDIATQLI SNMDINVILG GGRKYMFPAG TPDPEYPNDV NETGTRLDGK 250
NLVQEWLSKH QGSQYVWNRQ ELIQKSLDPS VTYLMGLFEP VDTKFEIQRD 300
PLMDPSLKDM TEAALHVLSR NPKGFYLFVE GGRIDRGHHL GTAYLALTEA 350
VMFDSAIERA SLQASEQDTL TIVTADHSHV FSFGGYTLRG TSIFGLAPLN 400
ALDGKPYTSI LYGNGPGYVG TGERPNVTDA ESHDPSYQQQ AAVPVKSETT 450
VGKDVAIFAR GPQAHLLHGV QEQNYIAHVM AFAGCLEPYT DCGLAPPADE 500
NRPTTPVQNS TTTTTTTTTT TTTTTTTRVQ NSASSLGPAT APLAWHYWPR 550
R 551
Length:551
Mass (Da):59,797
Last modified:October 1, 1996 - v1
Checksum:i486206A5A9A11C3B
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti29 – 291A → V.1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S66545 mRNA. Translation: AAB20378.2.
PIRiB56888.
S18408.
UniGeneiRn.138122.

Genome annotation databases

UCSCiRGD:621650. rat.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S66545 mRNA. Translation: AAB20378.2 .
PIRi B56888.
S18408.
UniGenei Rn.138122.

3D structure databases

ProteinModelPortali P51740.
SMRi P51740. Positions 20-498.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000026294.

Proteomic databases

PaxDbi P51740.
PRIDEi P51740.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:621650. rat.

Phylogenomic databases

eggNOGi COG1785.
HOVERGENi HBG007345.
PhylomeDBi P51740.

Gene expression databases

Genevestigatori P51740.

Family and domain databases

Gene3Di 3.40.720.10. 1 hit.
InterProi IPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view ]
Pfami PF00245. Alk_phosphatase. 1 hit.
[Graphical view ]
PRINTSi PR00113. ALKPHPHTASE.
SMARTi SM00098. alkPPc. 1 hit.
[Graphical view ]
SUPFAMi SSF53649. SSF53649. 1 hit.
PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Isolation of a mRNA that encodes a putative intestinal alkaline phosphatase regulated by 1,25-dihydroxyvitamin D-3."
    Strom M., Krisinger J., Deluca H.F.
    Biochim. Biophys. Acta 1090:299-304(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The two mRNAs encoding rat intestinal alkaline phosphatase represent two distinct nucleotide sequences."
    Engle M.J., Aleprs D.H.
    Clin. Chem. 38:2506-2509(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL NUCLEOTIDE SEQUENCE.
    Strain: Sprague-Dawley.
  3. "Purification and characterization of phytase from rat intestinal mucosa."
    Yang W.-J., Matsuda Y., Sano S., Masutani H., Nakagawa H.
    Biochim. Biophys. Acta 1075:75-82(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 20-29, VARIANT VAL-29.
  4. "Two rat intestinal alkaline phosphatase isoforms with different carboxyl-terminal peptides are both membrane-bound by a glycan phosphatidylinositol linkage."
    Engle M.J., Mahmood A., Alpers D.H.
    J. Biol. Chem. 270:11935-11940(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: GPI-ANCHOR.

Entry informationi

Entry nameiPPBI2_RAT
AccessioniPrimary (citable) accession number: P51740
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 9, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney). Rat has two genes for the intestinal isozyme.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi