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P51691

- ARS_PSEAE

UniProt

P51691 - ARS_PSEAE

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Protein

Arylsulfatase

Gene
atsA, PA0183
Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phenol sulfate + H2O = a phenol + sulfate.

Cofactori

Binds 1 calcium ion per subunit.

pH dependencei

Optimum pH is 8.9.

Temperature dependencei

Optimum temperature is 57 degrees Celsius. Incubation that exceeds 20 minutes above 50 degrees Celsius leads to enzyme inactivation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi13 – 131Calcium
Metal bindingi14 – 141Calcium
Metal bindingi51 – 511Calcium; via 3-oxoalanine
Active sitei115 – 1151
Metal bindingi317 – 3171Calcium
Metal bindingi318 – 3181Calcium

GO - Molecular functioni

  1. arylsulfatase activity Source: PseudoCAP
  2. metal ion binding Source: UniProtKB-KW
  3. phosphoric diester hydrolase activity Source: PseudoCAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Arylsulfatase (EC:3.1.6.1)
Short name:
AS
Alternative name(s):
Aryl-sulfate sulphohydrolase
Gene namesi
Name:atsA
Ordered Locus Names:PA0183
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438: Chromosome

Organism-specific databases

PseudoCAPiPA0183.

Subcellular locationi

Cytoplasm Reviewed prediction

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 536535ArylsulfatasePRO_0000192683Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei51 – 5113-oxoalanine (Cys)

Post-translational modificationi

The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity By similarity.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi208964.PA0183.

Structurei

Secondary structure

1
536
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 149
Helixi21 – 233
Helixi30 – 389
Beta strandi39 – 468
Helixi51 – 588
Helixi64 – 674
Helixi73 – 753
Turni78 – 825
Beta strandi87 – 893
Beta strandi92 – 943
Helixi97 – 1026
Turni103 – 1053
Beta strandi107 – 1137
Helixi120 – 1223
Turni124 – 1285
Beta strandi130 – 1356
Helixi155 – 1584
Beta strandi164 – 1663
Helixi180 – 19314
Beta strandi201 – 2066
Beta strandi211 – 2133
Helixi218 – 2214
Helixi222 – 2243
Turni225 – 2306
Helixi231 – 24515
Helixi265 – 2673
Helixi270 – 30334
Helixi307 – 3093
Beta strandi310 – 31910
Helixi325 – 3273
Helixi329 – 3313
Helixi335 – 3428
Helixi347 – 3493
Helixi360 – 3689
Beta strandi371 – 3733
Beta strandi377 – 3793
Helixi380 – 3834
Beta strandi387 – 3904
Beta strandi398 – 4014
Helixi407 – 4093
Helixi410 – 4189
Beta strandi425 – 4273
Helixi441 – 4444
Beta strandi447 – 4493
Beta strandi457 – 4626
Beta strandi465 – 4706
Beta strandi473 – 4775
Turni480 – 4823
Beta strandi485 – 4917
Turni492 – 4943
Turni503 – 5053
Helixi507 – 52418

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HDHX-ray1.30A/B1-536[»]
ProteinModelPortaliP51691.
SMRiP51691. Positions 3-527.

Miscellaneous databases

EvolutionaryTraceiP51691.

Family & Domainsi

Sequence similaritiesi

Belongs to the sulfatase family.

Phylogenomic databases

eggNOGiCOG3119.
HOGENOMiHOG000135353.
KOiK01130.
OMAiVPMRGKS.
OrthoDBiEOG61VZ37.
PhylomeDBiP51691.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR000917. Sulfatase.
IPR024607. Sulfatase_CS.
[Graphical view]
PfamiPF00884. Sulfatase. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00523. SULFATASE_1. 1 hit.
PS00149. SULFATASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P51691-1 [UniParc]FASTAAdd to Basket

« Hide

MSKRPNFLVI VADDLGFSDI GAFGGEIATP NLDALAIAGL RLTDFHTAST    50
CSPTRSMLLT GTDHHIAGIG TMAEALTPEL EGKPGYEGHL NERVVALPEL 100
LREAGYQTLM AGKWHLGLKP EQTPHARGFE RSFSLLPGAA NHYGFEPPYD 150
ESTPRILKGT PALYVEDERY LDTLPEGFYS SDAFGDKLLQ YLKERDQSRP 200
FFAYLPFSAP HWPLQAPREI VEKYRGRYDA GPEALRQERL ARLKELGLVE 250
ADVEAHPVLA LTREWEALED EERAKSARAM EVYAAMVERM DWNIGRVVDY 300
LRRQGELDNT FVLFMSDNGA EGALLEAFPK FGPDLLGFLD RHYDNSLENI 350
GRANSYVWYG PRWAQAATAP SRLYKAFTTQ GGIRVPALVR YPRLSRQGAI 400
SHAFATVMDV TPTLLDLAGV RHPGKRWRGR EIAEPRGRSW LGWLSGETEA 450
AHDENTVTGW ELFGMRAIRQ GDWKAVYLPA PVGPATWQLY DLARDPGEIH 500
DLADSQPGKL AELIEHWKRY VSETGVVEGA SPFLVR 536
Length:536
Mass (Da):59,946
Last modified:January 23, 2007 - v3
Checksum:iE926EC099E14EA63
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21S → D AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z48540 Genomic DNA. Translation: CAA88421.2.
AE004091 Genomic DNA. Translation: AAG03573.1.
PIRiD83622.
S69336.
RefSeqiNP_248873.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG03573; AAG03573; PA0183.
GeneIDi879288.
KEGGipae:PA0183.
PATRICi19834574. VBIPseAer58763_0189.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z48540 Genomic DNA. Translation: CAA88421.2 .
AE004091 Genomic DNA. Translation: AAG03573.1 .
PIRi D83622.
S69336.
RefSeqi NP_248873.1. NC_002516.2.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1HDH X-ray 1.30 A/B 1-536 [» ]
ProteinModelPortali P51691.
SMRi P51691. Positions 3-527.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 208964.PA0183.

Chemistry

BindingDBi P51691.
ChEMBLi CHEMBL5816.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG03573 ; AAG03573 ; PA0183 .
GeneIDi 879288.
KEGGi pae:PA0183.
PATRICi 19834574. VBIPseAer58763_0189.

Organism-specific databases

PseudoCAPi PA0183.

Phylogenomic databases

eggNOGi COG3119.
HOGENOMi HOG000135353.
KOi K01130.
OMAi VPMRGKS.
OrthoDBi EOG61VZ37.
PhylomeDBi P51691.

Miscellaneous databases

EvolutionaryTracei P51691.

Family and domain databases

Gene3Di 3.40.720.10. 1 hit.
InterProi IPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR000917. Sulfatase.
IPR024607. Sulfatase_CS.
[Graphical view ]
Pfami PF00884. Sulfatase. 1 hit.
[Graphical view ]
SUPFAMi SSF53649. SSF53649. 1 hit.
PROSITEi PS00523. SULFATASE_1. 1 hit.
PS00149. SULFATASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Purification and characterization of the arylsulfatase synthesized by Pseudomonas aeruginosa PAO during growth in sulfate-free medium and cloning of the arylsulfatase gene (atsA)."
    Beil S., Kehrli H., James P., Staudenmann W., Cook A.M., Leisinger T., Kertesz M.A.
    Eur. J. Biochem. 229:385-394(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  2. Kertesz M.A.
    Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  4. "1.3 A structure of arylsulfatase from Pseudomonas aeruginosa establishes the catalytic mechanism of sulfate ester cleavage in the sulfatase family."
    Boltes I., Czapinska H., Kahnert A., von Buelow R., Dierks T., Schmidt B., von Figura K., Kertesz M.A., Uson I.
    Structure 9:483-491(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.3 ANGSTROMS), OXOALANINE AT CYS-51.

Entry informationi

Entry nameiARS_PSEAE
AccessioniPrimary (citable) accession number: P51691
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 105 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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