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P51683 (CCR2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
C-C chemokine receptor type 2

Short name=C-C CKR-2
Short name=CC-CKR-2
Short name=CCR-2
Short name=CCR2
Alternative name(s):
JE/FIC receptor
MCP-1 receptor
CD_antigen=CD192
Gene names
Name:Ccr2
Synonyms:Cmkbr2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length373 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for the CCL2, CCL7 and CCL12 chemokines. Transduces a signal by increasing intracellular calcium ion levels. Ref.4

Subunit structure

Interacts with ARRB1 By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Detected in monocyte/macrophage cell lines, but not in nonhematopoietic cell lines.

Post-translational modification

Sulfation increases the affinity for both monomeric and dimeric CCL2 with stronger binding to the monomeric form. Binding of sulfated CCR2 to CCL2 promotes conversion of CCL2 from dimer to monomer By similarity.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processT-helper 17 cell chemotaxis

Inferred from direct assay PubMed 18586982. Source: BHF-UCL

angiogenesis

Traceable author statement PubMed 18334747. Source: BHF-UCL

apoptotic cell clearance

Inferred by curator PubMed 20691665. Source: BHF-UCL

blood vessel remodeling

Inferred from sequence or structural similarity. Source: BHF-UCL

cellular calcium ion homeostasis

Inferred from sequence or structural similarity. Source: BHF-UCL

cellular defense response

Inferred from mutant phenotype PubMed 9366570. Source: MGI

cellular homeostasis

Inferred from direct assay PubMed 18832716. Source: BHF-UCL

chemokine-mediated signaling pathway

Inferred from sequence or structural similarity. Source: BHF-UCL

hemopoiesis

Inferred from mutant phenotype PubMed 9366570. Source: MGI

humoral immune response

Inferred from mutant phenotype PubMed 10899907. Source: MGI

immune response

Inferred from mutant phenotype PubMed 10854218PubMed 10946288PubMed 10993920. Source: MGI

inflammatory response

Inferred from mutant phenotype PubMed 10946288. Source: MGI

negative regulation of angiogenesis

Inferred from mutant phenotype PubMed 14566334. Source: BHF-UCL

negative regulation of eosinophil degranulation

Inferred from mutant phenotype PubMed 14662900. Source: BHF-UCL

negative regulation of type 2 immune response

Inferred from mutant phenotype PubMed 14662900. Source: BHF-UCL

positive regulation of CD8-positive, alpha-beta T cell extravasation

Inferred from mutant phenotype PubMed 14662900. Source: BHF-UCL

positive regulation of T cell activation

Inferred from mutant phenotype PubMed 14662900. Source: BHF-UCL

positive regulation of T cell chemotaxis

Inferred from direct assay PubMed 18586982. Source: BHF-UCL

positive regulation of T-helper 1 cell differentiation

Inferred by curator PubMed 14662900. Source: BHF-UCL

positive regulation of T-helper 1 type immune response

Inferred from mutant phenotype PubMed 10657654. Source: BHF-UCL

positive regulation of alpha-beta T cell proliferation

Inferred from mutant phenotype PubMed 14662900. Source: BHF-UCL

positive regulation of astrocyte chemotaxis

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of hematopoietic stem cell migration

Inferred from mutant phenotype PubMed 20234092. Source: BHF-UCL

positive regulation of immune complex clearance by monocytes and macrophages

Inferred from mutant phenotype PubMed 14566334. Source: BHF-UCL

positive regulation of inflammatory response

Inferred from direct assay PubMed 20234092. Source: BHF-UCL

positive regulation of interferon-gamma production

Inferred from mutant phenotype PubMed 14662900. Source: BHF-UCL

positive regulation of interleukin-2 production

Inferred from mutant phenotype PubMed 14662900. Source: BHF-UCL

positive regulation of monocyte chemotaxis

Inferred from mutant phenotype PubMed 18832716. Source: BHF-UCL

positive regulation of monocyte extravasation

Inferred from mutant phenotype PubMed 14566334. Source: BHF-UCL

positive regulation of tumor necrosis factor biosynthetic process

Inferred from mutant phenotype PubMed 18832716. Source: BHF-UCL

regulation of cell migration

Inferred from mutant phenotype PubMed 10854218PubMed 10899907PubMed 10993920. Source: MGI

regulation of vascular endothelial growth factor production

Inferred from mutant phenotype PubMed 14566334. Source: BHF-UCL

sensory perception of pain

Inferred from mutant phenotype PubMed 12808141. Source: MGI

wound healing involved in inflammatory response

Non-traceable author statement PubMed 20234092. Source: BHF-UCL

   Cellular_componentcytosol

Inferred from sequence or structural similarity. Source: BHF-UCL

dendrite

Inferred from sequence or structural similarity. Source: BHF-UCL

external side of plasma membrane

Inferred from direct assay PubMed 23610400. Source: MGI

integral component of plasma membrane

Traceable author statement PubMed 20234092. Source: BHF-UCL

neuronal cell body

Inferred from sequence or structural similarity. Source: BHF-UCL

perikaryon

Inferred from sequence or structural similarity. Source: BHF-UCL

perinuclear region of cytoplasm

Inferred from sequence or structural similarity. Source: BHF-UCL

plasma membrane

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionC-C chemokine receptor activity

Inferred from direct assay Ref.1Ref.2. Source: MGI

chemokine (C-C motif) ligand 12 binding

Inferred from physical interaction Ref.4. Source: BHF-UCL

chemokine (C-C motif) ligand 2 binding

Inferred from physical interaction Ref.2. Source: BHF-UCL

chemokine (C-C motif) ligand 7 binding

Inferred from physical interaction Ref.2. Source: BHF-UCL

cytokine binding

Inferred from physical interaction Ref.1. Source: MGI

protein homodimerization activity

Inferred from sequence or structural similarity. Source: BHF-UCL

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 373373C-C chemokine receptor type 2
PRO_0000069234

Regions

Topological domain1 – 5555Extracellular Potential
Transmembrane56 – 8328Helical; Name=1; Potential
Topological domain84 – 9310Cytoplasmic Potential
Transmembrane94 – 11421Helical; Name=2; Potential
Topological domain115 – 12713Extracellular Potential
Transmembrane128 – 14922Helical; Name=3; Potential
Topological domain150 – 16617Cytoplasmic Potential
Transmembrane167 – 19125Helical; Name=4; Potential
Topological domain192 – 21928Extracellular Potential
Transmembrane220 – 23920Helical; Name=5; Potential
Topological domain240 – 25617Cytoplasmic Potential
Transmembrane257 – 28125Helical; Name=6; Potential
Topological domain282 – 29817Extracellular Potential
Transmembrane299 – 32224Helical; Name=7; Potential
Topological domain323 – 37351Cytoplasmic Potential

Amino acid modifications

Modified residue1521Phosphotyrosine; by JAK2 By similarity
Disulfide bond45 ↔ 290 By similarity
Disulfide bond126 ↔ 203 By similarity

Experimental info

Sequence conflict391Y → H in AAC52453. Ref.1
Sequence conflict1841A → G in AAC52453. Ref.1
Sequence conflict2641V → G in AAC52453. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P51683 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: FA012C10F4C9325A

FASTA37342,783
        10         20         30         40         50         60 
MEDNNMLPQF IHGILSTSHS LFTRSIQELD EGATTPYDYD DGEPCHKTSV KQIGAWILPP 

        70         80         90        100        110        120 
LYSLVFIFGF VGNMLVIIIL IGCKKLKSMT DIYLLNLAIS DLLFLLTLPF WAHYAANEWV 

       130        140        150        160        170        180 
FGNIMCKVFT GLYHIGYFGG IFFIILLTID RYLAIVHAVF ALKARTVTFG VITSVVTWVV 

       190        200        210        220        230        240 
AVFASLPGII FTKSKQDDHH YTCGPYFTQL WKNFQTIMRN ILSLILPLLV MVICYSGILH 

       250        260        270        280        290        300 
TLFRCRNEKK RHRAVRLIFA IMIVYFLFWT PYNIVLFLTT FQESLGMSNC VIDKHLDQAM 

       310        320        330        340        350        360 
QVTETLGMTH CCINPVIYAF VGEKFRRYLS IFFRKHIAKR LCKQCPVFYR ETADRVSSTF 

       370 
TPSTGEQEVS VGL 

« Hide

References

[1]"Molecular cloning and functional expression of murine JE (monocyte chemoattractant protein 1) and murine macrophage inflammatory protein 1alpha receptors: evidence for two closely linked C-C chemokine receptors on chromosome 9."
Boring L., Gosling J., Monteclaro F.S., Lusis A.J., Tsou C.-L., Charo I.F.
J. Biol. Chem. 271:7551-7558(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Cloning and functional expression of mCCR2, a murine receptor for the C-C chemokines JE and FIC."
Kurihara T., Bravo R.
J. Biol. Chem. 271:11603-11606(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
[3]"Mouse astrocytes respond to the chemokines MCP-1 and KC, but reverse transcriptase-polymerase chain reaction does not detect mRNA for the KC or new MCP-1 receptor."
Heesen M., Tanabe S., Berman M.A., Yoshizawa I., Luo Y., Kim R., Post T.W., Gerard C., Dorf M.E.
J. Neurosci. Res. 45:382-391(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"Murine monocyte chemoattractant protein (MCP)-5: a novel CC chemokine that is a structural and functional homologue of human MCP-1."
Sarafi M.N., Garcia-Zepeda E.A., MacLean J.A., Charo I.F., Luster A.D.
J. Exp. Med. 185:99-109(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS CCL12 RECEPTOR.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U47035 mRNA. Translation: AAC52453.1.
U51717 mRNA. Translation: AAC52557.1.
U56819 mRNA. Translation: AAC52784.1.
RefSeqNP_034045.1. NM_009915.2.
XP_006512489.1. XM_006512426.1.
XP_006512490.1. XM_006512427.1.
XP_006512491.1. XM_006512428.1.
UniGeneMm.6272.

3D structure databases

ProteinModelPortalP51683.
SMRP51683. Positions 44-334.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid198772. 3 interactions.

Chemistry

BindingDBP51683.
ChEMBLCHEMBL5412.
GuidetoPHARMACOLOGY59.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP51683.

Proteomic databases

PRIDEP51683.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000055918; ENSMUSP00000049909; ENSMUSG00000049103.
ENSMUST00000165984; ENSMUSP00000128734; ENSMUSG00000049103.
ENSMUST00000168841; ENSMUSP00000132453; ENSMUSG00000049103.
ENSMUST00000171719; ENSMUSP00000130112; ENSMUSG00000049103.
GeneID12772.
KEGGmmu:12772.
UCSCuc009shc.1. mouse.

Organism-specific databases

CTD729230.
MGIMGI:106185. Ccr2.

Phylogenomic databases

eggNOGNOG148353.
HOGENOMHOG000234122.
HOVERGENHBG106917.
InParanoidP51683.
KOK04177.
OMAGWKNFHT.
OrthoDBEOG738051.
PhylomeDBP51683.
TreeFamTF330966.

Gene expression databases

ArrayExpressP51683.
BgeeP51683.
CleanExMM_CCR2.
GenevestigatorP51683.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR002237. Chemokine_CCR2.
IPR000355. Chemokine_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERPTHR24227. PTHR24227. 1 hit.
PTHR24227:SF45. PTHR24227:SF45. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00657. CCCHEMOKINER.
PR01107. CHEMOKINER2.
PR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio282150.
PROP51683.
SOURCESearch...

Entry information

Entry nameCCR2_MOUSE
AccessionPrimary (citable) accession number: P51683
Secondary accession number(s): Q61172
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries