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P51683

- CCR2_MOUSE

UniProt

P51683 - CCR2_MOUSE

Protein

C-C chemokine receptor type 2

Gene

Ccr2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Receptor for the CCL2, CCL7 and CCL12 chemokines. Transduces a signal by increasing intracellular calcium ion levels.1 Publication

    GO - Molecular functioni

    1. C-C chemokine receptor activity Source: MGI
    2. chemokine (C-C motif) ligand 12 binding Source: BHF-UCL
    3. chemokine (C-C motif) ligand 2 binding Source: BHF-UCL
    4. chemokine (C-C motif) ligand 7 binding Source: BHF-UCL
    5. cytokine binding Source: MGI
    6. protein binding Source: MGI
    7. protein homodimerization activity Source: BHF-UCL

    GO - Biological processi

    1. angiogenesis Source: BHF-UCL
    2. apoptotic cell clearance Source: BHF-UCL
    3. blood vessel remodeling Source: BHF-UCL
    4. cellular calcium ion homeostasis Source: BHF-UCL
    5. cellular defense response Source: MGI
    6. cellular homeostasis Source: BHF-UCL
    7. chemokine-mediated signaling pathway Source: BHF-UCL
    8. hemopoiesis Source: MGI
    9. humoral immune response Source: MGI
    10. immune response Source: MGI
    11. inflammatory response Source: MGI
    12. monocyte chemotaxis Source: MGI
    13. negative regulation of angiogenesis Source: BHF-UCL
    14. negative regulation of eosinophil degranulation Source: BHF-UCL
    15. negative regulation of type 2 immune response Source: BHF-UCL
    16. positive regulation of alpha-beta T cell proliferation Source: BHF-UCL
    17. positive regulation of astrocyte chemotaxis Source: BHF-UCL
    18. positive regulation of CD8-positive, alpha-beta T cell extravasation Source: BHF-UCL
    19. positive regulation of hematopoietic stem cell migration Source: BHF-UCL
    20. positive regulation of immune complex clearance by monocytes and macrophages Source: BHF-UCL
    21. positive regulation of inflammatory response Source: BHF-UCL
    22. positive regulation of interferon-gamma production Source: BHF-UCL
    23. positive regulation of interleukin-2 production Source: BHF-UCL
    24. positive regulation of monocyte chemotaxis Source: BHF-UCL
    25. positive regulation of monocyte extravasation Source: BHF-UCL
    26. positive regulation of T cell activation Source: BHF-UCL
    27. positive regulation of T cell chemotaxis Source: BHF-UCL
    28. positive regulation of T-helper 1 cell differentiation Source: BHF-UCL
    29. positive regulation of T-helper 1 type immune response Source: BHF-UCL
    30. positive regulation of tumor necrosis factor biosynthetic process Source: BHF-UCL
    31. regulation of cell migration Source: MGI
    32. regulation of vascular endothelial growth factor production Source: BHF-UCL
    33. sensory perception of pain Source: MGI
    34. T-helper 17 cell chemotaxis Source: BHF-UCL
    35. wound healing involved in inflammatory response Source: BHF-UCL

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Enzyme and pathway databases

    ReactomeiREACT_199048. Beta defensins.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    C-C chemokine receptor type 2
    Short name:
    C-C CKR-2
    Short name:
    CC-CKR-2
    Short name:
    CCR-2
    Short name:
    CCR2
    Alternative name(s):
    JE/FIC receptor
    MCP-1 receptor
    CD_antigen: CD192
    Gene namesi
    Name:Ccr2
    Synonyms:Cmkbr2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:106185. Ccr2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: BHF-UCL
    2. dendrite Source: BHF-UCL
    3. external side of plasma membrane Source: MGI
    4. integral component of plasma membrane Source: BHF-UCL
    5. neuronal cell body Source: BHF-UCL
    6. perikaryon Source: BHF-UCL
    7. perinuclear region of cytoplasm Source: BHF-UCL
    8. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 373373C-C chemokine receptor type 2PRO_0000069234Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi45 ↔ 290PROSITE-ProRule annotation
    Disulfide bondi126 ↔ 203PROSITE-ProRule annotation
    Modified residuei152 – 1521Phosphotyrosine; by JAK2By similarity

    Post-translational modificationi

    Sulfation increases the affinity for both monomeric and dimeric CCL2 with stronger binding to the monomeric form. Binding of sulfated CCR2 to CCL2 promotes conversion of CCL2 from dimer to monomer By similarity.By similarity

    Keywords - PTMi

    Disulfide bond, Phosphoprotein

    Proteomic databases

    PRIDEiP51683.

    PTM databases

    PhosphoSiteiP51683.

    Expressioni

    Tissue specificityi

    Detected in monocyte/macrophage cell lines, but not in nonhematopoietic cell lines.

    Gene expression databases

    ArrayExpressiP51683.
    BgeeiP51683.
    CleanExiMM_CCR2.
    GenevestigatoriP51683.

    Interactioni

    Subunit structurei

    Interacts with ARRB1.By similarity

    Protein-protein interaction databases

    BioGridi198772. 3 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP51683.
    SMRiP51683. Positions 44-334.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 5555ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini84 – 9310CytoplasmicSequence Analysis
    Topological domaini115 – 12713ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini150 – 16617CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini192 – 21928ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini240 – 25617CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini282 – 29817ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini323 – 37351CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei56 – 8328Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei94 – 11421Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei128 – 14922Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei167 – 19125Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei220 – 23920Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei257 – 28125Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei299 – 32224Helical; Name=7Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG148353.
    HOGENOMiHOG000234122.
    HOVERGENiHBG106917.
    InParanoidiP51683.
    KOiK04177.
    OMAiGWKNFHT.
    OrthoDBiEOG738051.
    PhylomeDBiP51683.
    TreeFamiTF330966.

    Family and domain databases

    Gene3Di1.20.1070.10. 1 hit.
    InterProiIPR002237. Chemokine_CCR2.
    IPR000355. Chemokine_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view]
    PANTHERiPTHR24227. PTHR24227. 1 hit.
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00657. CCCHEMOKINER.
    PR01107. CHEMOKINER2.
    PR00237. GPCRRHODOPSN.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P51683-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEDNNMLPQF IHGILSTSHS LFTRSIQELD EGATTPYDYD DGEPCHKTSV    50
    KQIGAWILPP LYSLVFIFGF VGNMLVIIIL IGCKKLKSMT DIYLLNLAIS 100
    DLLFLLTLPF WAHYAANEWV FGNIMCKVFT GLYHIGYFGG IFFIILLTID 150
    RYLAIVHAVF ALKARTVTFG VITSVVTWVV AVFASLPGII FTKSKQDDHH 200
    YTCGPYFTQL WKNFQTIMRN ILSLILPLLV MVICYSGILH TLFRCRNEKK 250
    RHRAVRLIFA IMIVYFLFWT PYNIVLFLTT FQESLGMSNC VIDKHLDQAM 300
    QVTETLGMTH CCINPVIYAF VGEKFRRYLS IFFRKHIAKR LCKQCPVFYR 350
    ETADRVSSTF TPSTGEQEVS VGL 373
    Length:373
    Mass (Da):42,783
    Last modified:November 1, 1997 - v2
    Checksum:iFA012C10F4C9325A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti39 – 391Y → H in AAC52453. (PubMed:8631787)Curated
    Sequence conflicti184 – 1841A → G in AAC52453. (PubMed:8631787)Curated
    Sequence conflicti264 – 2641V → G in AAC52453. (PubMed:8631787)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U47035 mRNA. Translation: AAC52453.1.
    U51717 mRNA. Translation: AAC52557.1.
    U56819 mRNA. Translation: AAC52784.1.
    CCDSiCCDS23669.1.
    RefSeqiNP_034045.1. NM_009915.2.
    XP_006512489.1. XM_006512426.1.
    XP_006512490.1. XM_006512427.1.
    XP_006512491.1. XM_006512428.1.
    UniGeneiMm.6272.

    Genome annotation databases

    EnsembliENSMUST00000055918; ENSMUSP00000049909; ENSMUSG00000049103.
    ENSMUST00000165984; ENSMUSP00000128734; ENSMUSG00000049103.
    ENSMUST00000168841; ENSMUSP00000132453; ENSMUSG00000049103.
    ENSMUST00000171719; ENSMUSP00000130112; ENSMUSG00000049103.
    GeneIDi12772.
    KEGGimmu:12772.
    UCSCiuc009shc.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U47035 mRNA. Translation: AAC52453.1 .
    U51717 mRNA. Translation: AAC52557.1 .
    U56819 mRNA. Translation: AAC52784.1 .
    CCDSi CCDS23669.1.
    RefSeqi NP_034045.1. NM_009915.2.
    XP_006512489.1. XM_006512426.1.
    XP_006512490.1. XM_006512427.1.
    XP_006512491.1. XM_006512428.1.
    UniGenei Mm.6272.

    3D structure databases

    ProteinModelPortali P51683.
    SMRi P51683. Positions 44-334.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198772. 3 interactions.

    Chemistry

    BindingDBi P51683.
    ChEMBLi CHEMBL5412.
    GuidetoPHARMACOLOGYi 59.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P51683.

    Proteomic databases

    PRIDEi P51683.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000055918 ; ENSMUSP00000049909 ; ENSMUSG00000049103 .
    ENSMUST00000165984 ; ENSMUSP00000128734 ; ENSMUSG00000049103 .
    ENSMUST00000168841 ; ENSMUSP00000132453 ; ENSMUSG00000049103 .
    ENSMUST00000171719 ; ENSMUSP00000130112 ; ENSMUSG00000049103 .
    GeneIDi 12772.
    KEGGi mmu:12772.
    UCSCi uc009shc.1. mouse.

    Organism-specific databases

    CTDi 729230.
    MGIi MGI:106185. Ccr2.

    Phylogenomic databases

    eggNOGi NOG148353.
    HOGENOMi HOG000234122.
    HOVERGENi HBG106917.
    InParanoidi P51683.
    KOi K04177.
    OMAi GWKNFHT.
    OrthoDBi EOG738051.
    PhylomeDBi P51683.
    TreeFami TF330966.

    Enzyme and pathway databases

    Reactomei REACT_199048. Beta defensins.

    Miscellaneous databases

    NextBioi 282150.
    PROi P51683.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P51683.
    Bgeei P51683.
    CleanExi MM_CCR2.
    Genevestigatori P51683.

    Family and domain databases

    Gene3Di 1.20.1070.10. 1 hit.
    InterProi IPR002237. Chemokine_CCR2.
    IPR000355. Chemokine_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view ]
    PANTHERi PTHR24227. PTHR24227. 1 hit.
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00657. CCCHEMOKINER.
    PR01107. CHEMOKINER2.
    PR00237. GPCRRHODOPSN.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and functional expression of murine JE (monocyte chemoattractant protein 1) and murine macrophage inflammatory protein 1alpha receptors: evidence for two closely linked C-C chemokine receptors on chromosome 9."
      Boring L., Gosling J., Monteclaro F.S., Lusis A.J., Tsou C.-L., Charo I.F.
      J. Biol. Chem. 271:7551-7558(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Cloning and functional expression of mCCR2, a murine receptor for the C-C chemokines JE and FIC."
      Kurihara T., Bravo R.
      J. Biol. Chem. 271:11603-11606(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
    3. "Mouse astrocytes respond to the chemokines MCP-1 and KC, but reverse transcriptase-polymerase chain reaction does not detect mRNA for the KC or new MCP-1 receptor."
      Heesen M., Tanabe S., Berman M.A., Yoshizawa I., Luo Y., Kim R., Post T.W., Gerard C., Dorf M.E.
      J. Neurosci. Res. 45:382-391(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "Murine monocyte chemoattractant protein (MCP)-5: a novel CC chemokine that is a structural and functional homologue of human MCP-1."
      Sarafi M.N., Garcia-Zepeda E.A., MacLean J.A., Charo I.F., Luster A.D.
      J. Exp. Med. 185:99-109(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS CCL12 RECEPTOR.

    Entry informationi

    Entry nameiCCR2_MOUSE
    AccessioniPrimary (citable) accession number: P51683
    Secondary accession number(s): Q61172
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 119 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3