Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

C-C chemokine receptor type 2

Gene

Ccr2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for the CCL2, CCL7 and CCL12 chemokines. Transduces a signal by increasing intracellular calcium ion levels.1 Publication

GO - Molecular functioni

  1. C-C chemokine receptor activity Source: MGI
  2. chemokine (C-C motif) ligand 12 binding Source: BHF-UCL
  3. chemokine (C-C motif) ligand 2 binding Source: BHF-UCL
  4. chemokine (C-C motif) ligand 7 binding Source: BHF-UCL
  5. cytokine binding Source: MGI
  6. protein homodimerization activity Source: BHF-UCL

GO - Biological processi

  1. angiogenesis Source: BHF-UCL
  2. apoptotic cell clearance Source: BHF-UCL
  3. blood vessel remodeling Source: BHF-UCL
  4. cellular calcium ion homeostasis Source: BHF-UCL
  5. cellular defense response Source: MGI
  6. cellular homeostasis Source: BHF-UCL
  7. chemokine-mediated signaling pathway Source: BHF-UCL
  8. hemopoiesis Source: MGI
  9. humoral immune response Source: MGI
  10. immune response Source: MGI
  11. inflammatory response Source: MGI
  12. monocyte chemotaxis Source: MGI
  13. negative regulation of angiogenesis Source: BHF-UCL
  14. negative regulation of eosinophil degranulation Source: BHF-UCL
  15. negative regulation of type 2 immune response Source: BHF-UCL
  16. positive regulation of alpha-beta T cell proliferation Source: BHF-UCL
  17. positive regulation of astrocyte chemotaxis Source: BHF-UCL
  18. positive regulation of CD8-positive, alpha-beta T cell extravasation Source: BHF-UCL
  19. positive regulation of hematopoietic stem cell migration Source: BHF-UCL
  20. positive regulation of immune complex clearance by monocytes and macrophages Source: BHF-UCL
  21. positive regulation of inflammatory response Source: BHF-UCL
  22. positive regulation of interferon-gamma production Source: BHF-UCL
  23. positive regulation of interleukin-2 production Source: BHF-UCL
  24. positive regulation of monocyte chemotaxis Source: BHF-UCL
  25. positive regulation of monocyte extravasation Source: BHF-UCL
  26. positive regulation of T cell activation Source: BHF-UCL
  27. positive regulation of T cell chemotaxis Source: BHF-UCL
  28. positive regulation of T-helper 1 cell differentiation Source: BHF-UCL
  29. positive regulation of T-helper 1 type immune response Source: BHF-UCL
  30. positive regulation of tumor necrosis factor biosynthetic process Source: BHF-UCL
  31. regulation of cell migration Source: MGI
  32. regulation of vascular endothelial growth factor production Source: BHF-UCL
  33. sensory perception of pain Source: MGI
  34. T-helper 17 cell chemotaxis Source: BHF-UCL
  35. wound healing involved in inflammatory response Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_199048. Beta defensins.
REACT_230248. Chemokine receptors bind chemokines.
REACT_250376. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
C-C chemokine receptor type 2
Short name:
C-C CKR-2
Short name:
CC-CKR-2
Short name:
CCR-2
Short name:
CCR2
Alternative name(s):
JE/FIC receptor
MCP-1 receptor
CD_antigen: CD192
Gene namesi
Name:Ccr2
Synonyms:Cmkbr2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:106185. Ccr2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5555ExtracellularSequence AnalysisAdd
BLAST
Transmembranei56 – 8328Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini84 – 9310CytoplasmicSequence Analysis
Transmembranei94 – 11421Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini115 – 12713ExtracellularSequence AnalysisAdd
BLAST
Transmembranei128 – 14922Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini150 – 16617CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei167 – 19125Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini192 – 21928ExtracellularSequence AnalysisAdd
BLAST
Transmembranei220 – 23920Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini240 – 25617CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei257 – 28125Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini282 – 29817ExtracellularSequence AnalysisAdd
BLAST
Transmembranei299 – 32224Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini323 – 37351CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cytosol Source: BHF-UCL
  2. dendrite Source: BHF-UCL
  3. external side of plasma membrane Source: MGI
  4. integral component of plasma membrane Source: BHF-UCL
  5. neuronal cell body Source: BHF-UCL
  6. perikaryon Source: BHF-UCL
  7. perinuclear region of cytoplasm Source: BHF-UCL
  8. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 373373C-C chemokine receptor type 2PRO_0000069234Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi45 ↔ 290PROSITE-ProRule annotation
Disulfide bondi126 ↔ 203PROSITE-ProRule annotation
Modified residuei152 – 1521Phosphotyrosine; by JAK2By similarity

Post-translational modificationi

Sulfation increases the affinity for both monomeric and dimeric CCL2 with stronger binding to the monomeric form. Binding of sulfated CCR2 to CCL2 promotes conversion of CCL2 from dimer to monomer (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PRIDEiP51683.

PTM databases

PhosphoSiteiP51683.

Expressioni

Tissue specificityi

Detected in monocyte/macrophage cell lines, but not in nonhematopoietic cell lines.

Gene expression databases

BgeeiP51683.
CleanExiMM_CCR2.
ExpressionAtlasiP51683. baseline and differential.
GenevestigatoriP51683.

Interactioni

Subunit structurei

Interacts with ARRB1.By similarity

Protein-protein interaction databases

BioGridi198772. 3 interactions.

Structurei

3D structure databases

ProteinModelPortaliP51683.
SMRiP51683. Positions 44-334.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG148353.
HOGENOMiHOG000234122.
HOVERGENiHBG106917.
InParanoidiP51683.
KOiK04177.
OMAiGWKNFHT.
OrthoDBiEOG738051.
PhylomeDBiP51683.
TreeFamiTF330966.

Family and domain databases

InterProiIPR002237. Chemokine_CCR2.
IPR000355. Chemokine_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24227. PTHR24227. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00657. CCCHEMOKINER.
PR01107. CHEMOKINER2.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P51683-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEDNNMLPQF IHGILSTSHS LFTRSIQELD EGATTPYDYD DGEPCHKTSV
60 70 80 90 100
KQIGAWILPP LYSLVFIFGF VGNMLVIIIL IGCKKLKSMT DIYLLNLAIS
110 120 130 140 150
DLLFLLTLPF WAHYAANEWV FGNIMCKVFT GLYHIGYFGG IFFIILLTID
160 170 180 190 200
RYLAIVHAVF ALKARTVTFG VITSVVTWVV AVFASLPGII FTKSKQDDHH
210 220 230 240 250
YTCGPYFTQL WKNFQTIMRN ILSLILPLLV MVICYSGILH TLFRCRNEKK
260 270 280 290 300
RHRAVRLIFA IMIVYFLFWT PYNIVLFLTT FQESLGMSNC VIDKHLDQAM
310 320 330 340 350
QVTETLGMTH CCINPVIYAF VGEKFRRYLS IFFRKHIAKR LCKQCPVFYR
360 370
ETADRVSSTF TPSTGEQEVS VGL
Length:373
Mass (Da):42,783
Last modified:November 1, 1997 - v2
Checksum:iFA012C10F4C9325A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti39 – 391Y → H in AAC52453. (PubMed:8631787)Curated
Sequence conflicti184 – 1841A → G in AAC52453. (PubMed:8631787)Curated
Sequence conflicti264 – 2641V → G in AAC52453. (PubMed:8631787)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47035 mRNA. Translation: AAC52453.1.
U51717 mRNA. Translation: AAC52557.1.
U56819 mRNA. Translation: AAC52784.1.
CCDSiCCDS23669.1.
RefSeqiNP_034045.1. NM_009915.2.
XP_006512489.1. XM_006512426.1.
XP_006512490.1. XM_006512427.1.
XP_006512491.1. XM_006512428.1.
UniGeneiMm.6272.

Genome annotation databases

EnsembliENSMUST00000055918; ENSMUSP00000049909; ENSMUSG00000049103.
ENSMUST00000165984; ENSMUSP00000128734; ENSMUSG00000049103.
ENSMUST00000168841; ENSMUSP00000132453; ENSMUSG00000049103.
ENSMUST00000171719; ENSMUSP00000130112; ENSMUSG00000049103.
GeneIDi12772.
KEGGimmu:12772.
UCSCiuc009shc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47035 mRNA. Translation: AAC52453.1.
U51717 mRNA. Translation: AAC52557.1.
U56819 mRNA. Translation: AAC52784.1.
CCDSiCCDS23669.1.
RefSeqiNP_034045.1. NM_009915.2.
XP_006512489.1. XM_006512426.1.
XP_006512490.1. XM_006512427.1.
XP_006512491.1. XM_006512428.1.
UniGeneiMm.6272.

3D structure databases

ProteinModelPortaliP51683.
SMRiP51683. Positions 44-334.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198772. 3 interactions.

Chemistry

BindingDBiP51683.
ChEMBLiCHEMBL5412.
GuidetoPHARMACOLOGYi59.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP51683.

Proteomic databases

PRIDEiP51683.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000055918; ENSMUSP00000049909; ENSMUSG00000049103.
ENSMUST00000165984; ENSMUSP00000128734; ENSMUSG00000049103.
ENSMUST00000168841; ENSMUSP00000132453; ENSMUSG00000049103.
ENSMUST00000171719; ENSMUSP00000130112; ENSMUSG00000049103.
GeneIDi12772.
KEGGimmu:12772.
UCSCiuc009shc.1. mouse.

Organism-specific databases

CTDi729230.
MGIiMGI:106185. Ccr2.

Phylogenomic databases

eggNOGiNOG148353.
HOGENOMiHOG000234122.
HOVERGENiHBG106917.
InParanoidiP51683.
KOiK04177.
OMAiGWKNFHT.
OrthoDBiEOG738051.
PhylomeDBiP51683.
TreeFamiTF330966.

Enzyme and pathway databases

ReactomeiREACT_199048. Beta defensins.
REACT_230248. Chemokine receptors bind chemokines.
REACT_250376. G alpha (i) signalling events.

Miscellaneous databases

ChiTaRSiCcr2. mouse.
NextBioi282150.
PROiP51683.
SOURCEiSearch...

Gene expression databases

BgeeiP51683.
CleanExiMM_CCR2.
ExpressionAtlasiP51683. baseline and differential.
GenevestigatoriP51683.

Family and domain databases

InterProiIPR002237. Chemokine_CCR2.
IPR000355. Chemokine_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24227. PTHR24227. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00657. CCCHEMOKINER.
PR01107. CHEMOKINER2.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and functional expression of murine JE (monocyte chemoattractant protein 1) and murine macrophage inflammatory protein 1alpha receptors: evidence for two closely linked C-C chemokine receptors on chromosome 9."
    Boring L., Gosling J., Monteclaro F.S., Lusis A.J., Tsou C.-L., Charo I.F.
    J. Biol. Chem. 271:7551-7558(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning and functional expression of mCCR2, a murine receptor for the C-C chemokines JE and FIC."
    Kurihara T., Bravo R.
    J. Biol. Chem. 271:11603-11606(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  3. "Mouse astrocytes respond to the chemokines MCP-1 and KC, but reverse transcriptase-polymerase chain reaction does not detect mRNA for the KC or new MCP-1 receptor."
    Heesen M., Tanabe S., Berman M.A., Yoshizawa I., Luo Y., Kim R., Post T.W., Gerard C., Dorf M.E.
    J. Neurosci. Res. 45:382-391(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "Murine monocyte chemoattractant protein (MCP)-5: a novel CC chemokine that is a structural and functional homologue of human MCP-1."
    Sarafi M.N., Garcia-Zepeda E.A., MacLean J.A., Charo I.F., Luster A.D.
    J. Exp. Med. 185:99-109(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS CCL12 RECEPTOR.

Entry informationi

Entry nameiCCR2_MOUSE
AccessioniPrimary (citable) accession number: P51683
Secondary accession number(s): Q61172
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 1, 1997
Last modified: January 7, 2015
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.