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Protein

C-C chemokine receptor type 2

Gene

Ccr2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for the CCL2, CCL7 and CCL12 chemokines (PubMed:8996246). Receptor for the beta-defensin DEFB106A/DEFB106B (By similarity). Transduces a signal by increasing intracellular calcium ion levels (PubMed:8996246). Upon CCL2 ligation, mediates chemotaxis and migration induction through the activation of the PI3K cascade, the small G protein Rac and lamellipodium protrusion (By similarity).By similarity1 Publication

GO - Molecular functioni

  • C-C chemokine receptor activity Source: MGI
  • chemokine (C-C motif) ligand 12 binding Source: BHF-UCL
  • chemokine (C-C motif) ligand 2 binding Source: BHF-UCL
  • chemokine (C-C motif) ligand 7 binding Source: BHF-UCL
  • cytokine binding Source: MGI
  • protein homodimerization activity Source: BHF-UCL

GO - Biological processi

  • angiogenesis Source: BHF-UCL
  • apoptotic cell clearance Source: BHF-UCL
  • blood vessel remodeling Source: BHF-UCL
  • cellular calcium ion homeostasis Source: BHF-UCL
  • cellular defense response Source: MGI
  • cellular homeostasis Source: BHF-UCL
  • chemokine-mediated signaling pathway Source: BHF-UCL
  • hemopoiesis Source: MGI
  • homeostasis of number of cells within a tissue Source: MGI
  • humoral immune response Source: MGI
  • immune response Source: MGI
  • inflammatory response Source: MGI
  • leukocyte adhesion to vascular endothelial cell Source: MGI
  • monocyte chemotaxis Source: MGI
  • negative regulation of angiogenesis Source: BHF-UCL
  • negative regulation of eosinophil degranulation Source: BHF-UCL
  • negative regulation of type 2 immune response Source: BHF-UCL
  • neutrophil clearance Source: MGI
  • positive regulation of alpha-beta T cell proliferation Source: BHF-UCL
  • positive regulation of astrocyte chemotaxis Source: BHF-UCL
  • positive regulation of CD8-positive, alpha-beta T cell extravasation Source: BHF-UCL
  • positive regulation of hematopoietic stem cell migration Source: BHF-UCL
  • positive regulation of immune complex clearance by monocytes and macrophages Source: BHF-UCL
  • positive regulation of inflammatory response Source: BHF-UCL
  • positive regulation of interferon-gamma production Source: BHF-UCL
  • positive regulation of interleukin-2 production Source: BHF-UCL
  • positive regulation of leukocyte tethering or rolling Source: MGI
  • positive regulation of monocyte chemotaxis Source: BHF-UCL
  • positive regulation of monocyte extravasation Source: BHF-UCL
  • positive regulation of T cell activation Source: BHF-UCL
  • positive regulation of T cell chemotaxis Source: BHF-UCL
  • positive regulation of T-helper 1 cell differentiation Source: BHF-UCL
  • positive regulation of T-helper 1 type immune response Source: BHF-UCL
  • positive regulation of tumor necrosis factor biosynthetic process Source: BHF-UCL
  • regulation of cell migration Source: MGI
  • regulation of vascular endothelial growth factor production Source: BHF-UCL
  • sensory perception of pain Source: MGI
  • T-helper 17 cell chemotaxis Source: BHF-UCL
  • wound healing involved in inflammatory response Source: BHF-UCL

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-MMU-1461957. Beta defensins.
R-MMU-380108. Chemokine receptors bind chemokines.
R-MMU-418594. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
C-C chemokine receptor type 2
Short name:
C-C CKR-2
Short name:
CC-CKR-2
Short name:
CCR-2
Short name:
CCR2
Alternative name(s):
JE/FIC receptor
MCP-1 receptor
CD_antigen: CD192
Gene namesi
Name:Ccr2
Synonyms:Cmkbr2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:106185. Ccr2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 55ExtracellularSequence analysisAdd BLAST55
Transmembranei56 – 83Helical; Name=1Sequence analysisAdd BLAST28
Topological domaini84 – 93CytoplasmicSequence analysis10
Transmembranei94 – 114Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini115 – 127ExtracellularSequence analysisAdd BLAST13
Transmembranei128 – 149Helical; Name=3Sequence analysisAdd BLAST22
Topological domaini150 – 166CytoplasmicSequence analysisAdd BLAST17
Transmembranei167 – 191Helical; Name=4Sequence analysisAdd BLAST25
Topological domaini192 – 219ExtracellularSequence analysisAdd BLAST28
Transmembranei220 – 239Helical; Name=5Sequence analysisAdd BLAST20
Topological domaini240 – 256CytoplasmicSequence analysisAdd BLAST17
Transmembranei257 – 281Helical; Name=6Sequence analysisAdd BLAST25
Topological domaini282 – 298ExtracellularSequence analysisAdd BLAST17
Transmembranei299 – 322Helical; Name=7Sequence analysisAdd BLAST24
Topological domaini323 – 373CytoplasmicSequence analysisAdd BLAST51

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5412.
GuidetoPHARMACOLOGYi59.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000692341 – 373C-C chemokine receptor type 2Add BLAST373

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi126 ↔ 203PROSITE-ProRule annotation
Modified residuei152Phosphotyrosine; by JAK2By similarity1

Post-translational modificationi

Sulfation increases the affinity for both monomeric and dimeric CCL2 with stronger binding to the monomeric form. Binding of sulfated CCR2 to CCL2 promotes conversion of CCL2 from dimer to monomer.By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

EPDiP51683.
PaxDbiP51683.
PRIDEiP51683.

PTM databases

iPTMnetiP51683.
PhosphoSitePlusiP51683.

Expressioni

Tissue specificityi

Detected in monocyte/macrophage cell lines, but not in nonhematopoietic cell lines.

Gene expression databases

BgeeiENSMUSG00000049103.
CleanExiMM_CCR2.
ExpressionAtlasiP51683. baseline and differential.
GenevisibleiP51683. MM.

Interactioni

Subunit structurei

Interacts with ARRB1. Interacts (via extracellular N-terminal region) with beta-defensin DEFB106A/DEFB106B; this interaction may preferentially require specific tyrosine sulfation on CCR2. Interact with NUP85; the interaction is required for CCR2 clusters formation on the cell membrane and CCR2 signaling (By similarity).By similarity

GO - Molecular functioni

  • chemokine (C-C motif) ligand 12 binding Source: BHF-UCL
  • chemokine (C-C motif) ligand 2 binding Source: BHF-UCL
  • chemokine (C-C motif) ligand 7 binding Source: BHF-UCL
  • cytokine binding Source: MGI
  • protein homodimerization activity Source: BHF-UCL

Protein-protein interaction databases

BioGridi198772. 3 interactors.
STRINGi10090.ENSMUSP00000049909.

Chemistry databases

BindingDBiP51683.

Structurei

3D structure databases

ProteinModelPortaliP51683.
SMRiP51683.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118785.
HOGENOMiHOG000234122.
HOVERGENiHBG106917.
InParanoidiP51683.
KOiK04177.
OMAiCPVFYRE.
OrthoDBiEOG091G0B7A.
PhylomeDBiP51683.
TreeFamiTF330966.

Family and domain databases

InterProiView protein in InterPro
IPR002237. Chemokine_CCR2.
IPR000355. Chemokine_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
PfamiView protein in Pfam
PF00001. 7tm_1. 1 hit.
PRINTSiPR00657. CCCHEMOKINER.
PR01107. CHEMOKINER2.
PR00237. GPCRRHODOPSN.
PROSITEiView protein in PROSITE
PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P51683-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDNNMLPQF IHGILSTSHS LFTRSIQELD EGATTPYDYD DGEPCHKTSV
60 70 80 90 100
KQIGAWILPP LYSLVFIFGF VGNMLVIIIL IGCKKLKSMT DIYLLNLAIS
110 120 130 140 150
DLLFLLTLPF WAHYAANEWV FGNIMCKVFT GLYHIGYFGG IFFIILLTID
160 170 180 190 200
RYLAIVHAVF ALKARTVTFG VITSVVTWVV AVFASLPGII FTKSKQDDHH
210 220 230 240 250
YTCGPYFTQL WKNFQTIMRN ILSLILPLLV MVICYSGILH TLFRCRNEKK
260 270 280 290 300
RHRAVRLIFA IMIVYFLFWT PYNIVLFLTT FQESLGMSNC VIDKHLDQAM
310 320 330 340 350
QVTETLGMTH CCINPVIYAF VGEKFRRYLS IFFRKHIAKR LCKQCPVFYR
360 370
ETADRVSSTF TPSTGEQEVS VGL
Length:373
Mass (Da):42,783
Last modified:November 1, 1997 - v2
Checksum:iFA012C10F4C9325A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti39Y → H in AAC52453 (PubMed:8631787).Curated1
Sequence conflicti184A → G in AAC52453 (PubMed:8631787).Curated1
Sequence conflicti264V → G in AAC52453 (PubMed:8631787).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47035 mRNA. Translation: AAC52453.1.
U51717 mRNA. Translation: AAC52557.1.
U56819 mRNA. Translation: AAC52784.1.
CCDSiCCDS23669.1.
RefSeqiNP_034045.1. NM_009915.2.
XP_006512491.1. XM_006512428.2.
XP_011241365.1. XM_011243063.1.
XP_011241366.1. XM_011243064.2.
UniGeneiMm.6272.

Genome annotation databases

EnsembliENSMUST00000055918; ENSMUSP00000049909; ENSMUSG00000049103.
ENSMUST00000165984; ENSMUSP00000128734; ENSMUSG00000049103.
ENSMUST00000168841; ENSMUSP00000132453; ENSMUSG00000049103.
ENSMUST00000171719; ENSMUSP00000130112; ENSMUSG00000049103.
GeneIDi12772.
KEGGimmu:12772.
UCSCiuc009shc.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiCCR2_MOUSE
AccessioniPrimary (citable) accession number: P51683
Secondary accession number(s): Q61172
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 1, 1997
Last modified: November 22, 2017
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families