Reviewed, UniProtKB/Swiss-Prot P51682 (CCR5_MOUSE)

Names and origin

Protein names

Recommended name:
    C-C chemokine receptor type 5
      Short name=C-C CKR-5
      Short name=CC-CKR-5
      Short name=CCR-5
Alternative name(s):
    MIP-1 alpha receptor
    CD_antigen=CD195

Gene names

Name:	Ccr5
Synonyms:	Cmkbr5

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Receptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation By similarity.
Subunit structure	Interacts with PRAF2. Efficient ligand binding to CCL3/MIP-1alpha and CCR4/MIP-1beta requires sulfation, O-glycosylation and sialic acid modifications. Glycosylation on Ser-6 is required for efficient binding of CCL4. Interacts with ADRBK1 By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Post-translational modification	Sulfated on at least 2 of the N-terminal tyrosines. Sulfation is required for efficient binding of the chemokines, CCL3 and CCL4 By similarity. O-glycosylated, but not N-glycosylated. Ser-6 appears to be the major site. Also sialylated glycans present which contribute to chemokine binding By similarity. Palmitoylation in the C-terminal is important for cell surface expression By similarity. Phosphorylation on serine residues in the C-terminal is stimulated by binding CC chemokines especially by APO-RANTES By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate Phosphoprotein Sulfation
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	external side of plasma membrane Inferred from direct assay. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	C-C chemokine receptor activity Ref.1 Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	C-C chemokine receptor type 5		PRO_0000069269
Regions
	Topological domain	1 – 32	32	Extracellular Potential
	Transmembrane	33 – 60	28	1 Potential
	Topological domain	61 – 70	10	Cytoplasmic Potential
	Transmembrane	71 – 91	21	2 Potential
	Topological domain	92 – 104	13	Extracellular Potential
	Transmembrane	105 – 126	22	3 Potential
	Topological domain	127 – 143	17	Cytoplasmic Potential
	Transmembrane	144 – 168	25	4 Potential
	Topological domain	169 – 200	32	Extracellular Potential
	Transmembrane	201 – 220	20	5 Potential
	Topological domain	221 – 237	17	Cytoplasmic Potential
	Transmembrane	238 – 262	25	6 Potential
	Topological domain	263 – 279	17	Extracellular Potential
	Transmembrane	280 – 303	24	7 Potential
	Topological domain	304 – 354	51	Cytoplasmic Potential
Amino acid modifications
	Modified residue	10	1	Sulfotyrosine Potential
	Modified residue	12	1	Sulfotyrosine Potential
	Modified residue	16	1	Sulfotyrosine Potential
	Modified residue	338	1	Phosphoserine; by BARK1 By similarity
	Modified residue	339	1	Phosphoserine; by BARK1 By similarity
	Modified residue	344	1	Phosphoserine; by BARK1 By similarity
	Modified residue	351	1	Phosphoserine; by BARK1 By similarity
	Lipidation	323	1	S-palmitoyl cysteine By similarity
	Lipidation	326	1	S-palmitoyl cysteine By similarity
	Glycosylation	6	1	O-linked (GalNAc...) By similarity
	Disulfide bond	103 ↔ 180		By similarity
Natural variations
	Natural variant	11	1	I → S
	Natural variant	62	1	K → R
	Natural variant	66	1	V → M
	Natural variant	97	1	I → V
	Natural variant	109	1	V → L
	Natural variant	156	1	V → A
	Natural variant	160	1	F → S
	Natural variant	185	1	P → L
	Natural variant	213	1	I → V
	Natural variant	318	1	I → M
	Natural variant	337	1	V → A
Experimental info
	Sequence conflict	3	1	F → L in CAA63867. Ref.2
	Sequence conflict	80	1	L → F in CAA63867. Ref.2
	Sequence conflict	145	1	N → I in AAB71183. Ref.5
	Sequence conflict	190	1	H → Y in AAB37273. Ref.3
	Sequence conflict	208	1	P → S in AAC52454. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P51682-1 [UniParc]. Last modified July 15, 1998. Version 2. Checksum: B4A6B942E88F9CF0	FASTA	354	40,863
	10 20 30 40 50 60 MDFQGSVPTY IYDIDYGMSA PCQKINVKQI AAQLLPPLYS LVFIFGFVGN MMVFLILISC 70 80 90 100 110 120 KKLKSVTDIY LLNLAISDLL FLLTLPFWAH YAANEWIFGN IMCKVFTGVY HIGYFGGIFF 130 140 150 160 170 180 IILLTIDRYL AIVHAVFALK VRTVNFGVIT SVVTWVVAVF ASLPEIIFTR SQKEGFHYTC 190 200 210 220 230 240 SPHFPHTQYH FWKSFQTLKM VILSLILPLL VMIICYSGIL HTLFRCRNEK KRHRAVRLIF 250 260 270 280 290 300 AIMIVYFLFW TPYNIVLLLT TFQEFFGLNN CSSSNRLDQA MQATETLGMT HCCLNPVIYA 310 320 330 340 350 FVGEKFRSYL SVFFRKHIVK RFCKRCSIFQ QDNPDRVSSV YTRSTGEHEV STGL

References

[1]	"Molecular cloning and functional expression of murine JE (monocyte chemoattractant protein 1) and murine macrophage inflammatory protein 1alpha receptors: evidence for two closely linked C-C chemokine receptors on chromosome 9." Boring L., Gosling J., Monteclaro F.S., Lusis A.J., Tsou C.-L., Charo I.F. J. Biol. Chem. 271:7551-7558(1996) [PubMed: 8631787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: 129/SvJ. Tissue: Spleen.
[2]	"Cloning and characterization of a novel murine macrophage inflammatory protein-1 alpha receptor." Meyer A., Coyle A.J., Proudfoot A.E.I., Wells T.N.C., Power C.A. J. Biol. Chem. 271:14445-14451(1996) [PubMed: 8662890] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6 X CBA. Tissue: Thymus.
[3]	Kuziel W.A., Beck M.A., Dawson T.C., Maeda N. Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Ola.
[4]	"Polymorphisms in the CCR5 genes of African green monkeys and mice implicate specific amino acids in infections by simian and human immunodeficiency viruses." Kuhmann S.E., Platt E.J., Kozak S.L., Kabat D. J. Virol. 71:8642-8656(1997) [PubMed: 9343222] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C57BL/6 and NIH Swiss. Tissue: Kidney, Liver and Spleen.
[5]	"Two distinct CCR5 domains can mediate coreceptor usage by human immunodeficiency virus type 1." Doranz B.J., Lu Z.H., Rucker J., Zhang T.Y., Sharron M., Cen Y.H., Wang Z.X., Guo H.H., Du J.G., Accavitti M.A., Doms R.W., Peiper S.C. J. Virol. 71:6305-6314(1997) [PubMed: 9261347] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129.
[6]	Guo B., Kuno K., Harada A., Matsushima K. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U47036 mRNA. Translation: AAC52454.1. X94151 mRNA. Translation: CAA63867.1. U68565 Genomic DNA. Translation: AAB37273.1. U83327 Genomic DNA. Translation: AAC53386.1. AF022990 Genomic DNA. Translation: AAC53389.1. AF019772 Genomic DNA. Translation: AAB71183.1. D83648 mRNA. Translation: BAA12024.1.
IPI	IPI00312494.
UniGene	Mm.14302
3D structure databases
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P51682.
Genome annotation databases
Ensembl	ENSMUSG00000079227. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:107182. Ccr5.
Phylogenomic databases
HOGENOM	P51682.
HOVERGEN	P51682.
Gene expression databases
ArrayExpress	P51682.
Bgee	P51682.
CleanEx	MM_CCR5.
GermOnline	ENSMUSG00000073993. Mus musculus. ENSMUSG00000075434. Mus musculus.
Family and domain databases
InterPro	IPR000276. 7TM_GPCR_Rhodpsn. IPR002240. CC_5_rcpt. IPR000355. Chmkine_rcpt. IPR017452. GPCR_Rhodpsn_supfam. [Graphical view]
PANTHER	PTHR19264:SF204. CC_5_rcpt. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00657. CCCHEMOKINER. PR01110. CHEMOKINER5. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

CCR5_MOUSE

Accession

Primary (citable) accession number: P51682
Secondary accession number(s): O35313 , O35891, P97308, P97405, Q61867

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	July 15, 1998
Last modified:	June 16, 2009
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)