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P51682 (CCR5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
C-C chemokine receptor type 5

Short name=C-C CKR-5
Short name=CC-CKR-5
Short name=CCR-5
Alternative name(s):
MIP-1 alpha receptor
CD_antigen=CD195
Gene names
Name:Ccr5
Synonyms:Cmkbr5
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length354 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Receptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation By similarity.

Subunit structure

Interacts with PRAF2. Efficient ligand binding to CCL3/MIP-1alpha and CCL4/MIP-1beta requires sulfation, O-glycosylation and sialic acid modifications. Glycosylation on Ser-6 is required for efficient binding of CCL4. Interacts with ADRBK1. Interacts with ARRB1 and ARRB2 By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Post-translational modification

Sulfated on at least 2 of the N-terminal tyrosines. Sulfation is required for efficient binding of the chemokines, CCL3 and CCL4 By similarity.

O-glycosylated, but not N-glycosylated. Ser-6 appears to be the major site. Also sialylated glycans present which contribute to chemokine binding By similarity.

Palmitoylation in the C-terminal is important for cell surface expression By similarity.

Phosphorylation on serine residues in the C-terminal is stimulated by binding CC chemokines especially by APO-RANTES By similarity.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 354354C-C chemokine receptor type 5
PRO_0000069269

Regions

Topological domain1 – 3232Extracellular Potential
Transmembrane33 – 6028Helical; Name=1; Potential
Topological domain61 – 7010Cytoplasmic Potential
Transmembrane71 – 9121Helical; Name=2; Potential
Topological domain92 – 10413Extracellular Potential
Transmembrane105 – 12622Helical; Name=3; Potential
Topological domain127 – 14317Cytoplasmic Potential
Transmembrane144 – 16825Helical; Name=4; Potential
Topological domain169 – 20032Extracellular Potential
Transmembrane201 – 22020Helical; Name=5; Potential
Topological domain221 – 23717Cytoplasmic Potential
Transmembrane238 – 26225Helical; Name=6; Potential
Topological domain263 – 27917Extracellular Potential
Transmembrane280 – 30324Helical; Name=7; Potential
Topological domain304 – 35451Cytoplasmic Potential

Amino acid modifications

Modified residue101Sulfotyrosine Potential
Modified residue121Sulfotyrosine Potential
Modified residue161Sulfotyrosine Potential
Modified residue3381Phosphoserine; by BARK1 By similarity
Modified residue3391Phosphoserine; by BARK1 By similarity
Modified residue3441Phosphoserine; by BARK1 By similarity
Modified residue3511Phosphoserine; by BARK1 By similarity
Lipidation3231S-palmitoyl cysteine By similarity
Lipidation3261S-palmitoyl cysteine By similarity
Glycosylation61O-linked (GalNAc...) By similarity
Disulfide bond103 ↔ 180 By similarity

Natural variations

Natural variant111S → I.
Natural variant971V → I.
Natural variant1091L → V.
Natural variant1561A → V.
Natural variant2131V → I.
Natural variant3181M → I.
Natural variant3371A → V.

Experimental info

Sequence conflict31F → L in CAA63867. Ref.2
Sequence conflict621K → R in AAC53386. Ref.4
Sequence conflict661V → M in AAC53386. Ref.4
Sequence conflict801L → F in CAA63867. Ref.2
Sequence conflict1451N → I in AAB71183. Ref.5
Sequence conflict1601F → S in AAC53386. Ref.4
Sequence conflict1851P → L in AAC53386. Ref.4
Sequence conflict1901H → Y in AAB37273. Ref.3
Sequence conflict2081P → S in AAC52454. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P51682 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: D91F50EC9C956795

FASTA35440,785
        10         20         30         40         50         60 
MDFQGSVPTY SYDIDYGMSA PCQKINVKQI AAQLLPPLYS LVFIFGFVGN MMVFLILISC 

        70         80         90        100        110        120 
KKLKSVTDIY LLNLAISDLL FLLTLPFWAH YAANEWVFGN IMCKVFTGLY HIGYFGGIFF 

       130        140        150        160        170        180 
IILLTIDRYL AIVHAVFALK VRTVNFGVIT SVVTWAVAVF ASLPEIIFTR SQKEGFHYTC 

       190        200        210        220        230        240 
SPHFPHTQYH FWKSFQTLKM VILSLILPLL VMVICYSGIL HTLFRCRNEK KRHRAVRLIF 

       250        260        270        280        290        300 
AIMIVYFLFW TPYNIVLLLT TFQEFFGLNN CSSSNRLDQA MQATETLGMT HCCLNPVIYA 

       310        320        330        340        350 
FVGEKFRSYL SVFFRKHMVK RFCKRCSIFQ QDNPDRASSV YTRSTGEHEV STGL 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and functional expression of murine JE (monocyte chemoattractant protein 1) and murine macrophage inflammatory protein 1alpha receptors: evidence for two closely linked C-C chemokine receptors on chromosome 9."
Boring L., Gosling J., Monteclaro F.S., Lusis A.J., Tsou C.-L., Charo I.F.
J. Biol. Chem. 271:7551-7558(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: 129/SvJ.
Tissue: Spleen.
[2]"Cloning and characterization of a novel murine macrophage inflammatory protein-1 alpha receptor."
Meyer A., Coyle A.J., Proudfoot A.E.I., Wells T.N.C., Power C.A.
J. Biol. Chem. 271:14445-14451(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6 X CBA.
Tissue: Thymus.
[3]Kuziel W.A., Beck M.A., Dawson T.C., Maeda N.
Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/Ola.
[4]"Polymorphisms in the CCR5 genes of African green monkeys and mice implicate specific amino acids in infections by simian and human immunodeficiency viruses."
Kuhmann S.E., Platt E.J., Kozak S.L., Kabat D.
J. Virol. 71:8642-8656(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: C57BL/6 and NIH Swiss.
Tissue: Kidney, Liver and Spleen.
[5]"Two distinct CCR5 domains can mediate coreceptor usage by human immunodeficiency virus type 1."
Doranz B.J., Lu Z.H., Rucker J., Zhang T.Y., Sharron M., Cen Y.H., Wang Z.X., Guo H.H., Du J.G., Accavitti M.A., Doms R.W., Peiper S.C.
J. Virol. 71:6305-6314(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129.
[6]Guo B., Kuno K., Harada A., Matsushima K.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[7]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J and NOD.
Tissue: Spinal ganglion.
[8]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U47036 mRNA. Translation: AAC52454.1.
X94151 mRNA. Translation: CAA63867.1.
U68565 Genomic DNA. Translation: AAB37273.1.
U83327 Genomic DNA. Translation: AAC53386.1.
AF022990 Genomic DNA. Translation: AAC53389.1.
AF019772 Genomic DNA. Translation: AAB71183.1.
D83648 mRNA. Translation: BAA12024.1.
AK141906 mRNA. Translation: BAE24879.1.
AK154595 mRNA. Translation: BAE32698.1.
AK155628 mRNA. Translation: BAE33354.1.
AK155867 mRNA. Translation: BAE33471.1.
CH466671 Genomic DNA. Translation: EDL37177.1.
BC103574 mRNA. Translation: AAI03575.1.
BC103586 mRNA. Translation: AAI03587.1.
BC103587 mRNA. Translation: AAI03588.1.
RefSeqNP_034047.2. NM_009917.5.
UniGeneMm.14302.

3D structure databases

ProteinModelPortalP51682.
SMRP51682. Positions 21-315.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBP51682.
ChEMBLCHEMBL3676.
GuidetoPHARMACOLOGY62.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP51682.

Proteomic databases

PRIDEP51682.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000111442; ENSMUSP00000107069; ENSMUSG00000079227.
GeneID12774.
KEGGmmu:12774.
UCSCuc009shd.2. mouse.

Organism-specific databases

CTD1234.
MGIMGI:107182. Ccr5.

Phylogenomic databases

eggNOGNOG148353.
GeneTreeENSGT00700000104407.
HOVERGENHBG106917.
InParanoidQ3ZAZ8.
KOK04180.
OMAIIFTRSQ.
OrthoDBEOG738051.
TreeFamTF330966.

Gene expression databases

ArrayExpressP51682.
BgeeP51682.
CleanExMM_CCR5.
GenevestigatorP51682.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR002240. Chemokine_CCR5.
IPR000355. Chemokine_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERPTHR24227. PTHR24227. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00657. CCCHEMOKINER.
PR01110. CHEMOKINER5.
PR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio282158.
PROP51682.
SOURCESearch...

Entry information

Entry nameCCR5_MOUSE
AccessionPrimary (citable) accession number: P51682
Secondary accession number(s): O35313 expand/collapse secondary AC list , O35891, P97308, P97405, Q3ZAZ8, Q61867
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries