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Protein

C-C chemokine receptor type 4

Gene

Ccr4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

High affinity receptor for the C-C type chemokines CCL17/TARC and CCL22/MDC. The activity of this receptor is mediated by G(i) proteins which activate a phosphatidylinositol-calcium second messenger system. Could play a role in lipopolysaccharide (LPS)-induced endotoxic shock. In the CNS, could mediate hippocampal-neuron survival.1 Publication

GO - Molecular functioni

  • C-C chemokine receptor activity Source: MGI

GO - Biological processi

  • chemokine-mediated signaling pathway Source: GOC
  • chemotaxis Source: MGI
  • immune response Source: InterPro
  • inflammatory response Source: MGI
  • neuron migration Source: MGI
  • tolerance induction Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_288802. Chemokine receptors bind chemokines.
REACT_331048. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
C-C chemokine receptor type 4
Short name:
C-C CKR-4
Short name:
CC-CKR-4
Short name:
CCR-4
Short name:
CCR4
Alternative name(s):
CD_antigen: CD194
Gene namesi
Name:Ccr4
Synonyms:Cmkbr4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:107824. Ccr4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3939ExtracellularSequence AnalysisAdd
BLAST
Transmembranei40 – 6728Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini68 – 7710CytoplasmicSequence Analysis
Transmembranei78 – 9821Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini99 – 11113ExtracellularSequence AnalysisAdd
BLAST
Transmembranei112 – 13322Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini134 – 15017CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei151 – 17525Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini176 – 20631ExtracellularSequence AnalysisAdd
BLAST
Transmembranei207 – 22620Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini227 – 24216CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei243 – 26725Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini268 – 28417ExtracellularSequence AnalysisAdd
BLAST
Transmembranei285 – 30824Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini309 – 36052CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • external side of plasma membrane Source: MGI
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 360360C-C chemokine receptor type 4PRO_0000069246Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi2 – 21N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi110 ↔ 187PROSITE-ProRule annotation
Glycosylationi183 – 1831N-linked (GlcNAc...)Sequence Analysis
Glycosylationi194 – 1941N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

In natural killer cells, CCL22 binding induces phosphorylation on yet undefined Ser/Thr residues, most probably by beta-adrenergic receptor kinases 1 and 2.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiP51680.

Expressioni

Tissue specificityi

Expressed in the thymus, macrophages and T- and B-cells.

Developmental stagei

Low expression at 7.5 dpc and 12.5 dpc in the yolk sac.

Gene expression databases

BgeeiP51680.
CleanExiMM_CCR4.
GenevisibleiP51680. MM.

Interactioni

Protein-protein interaction databases

MINTiMINT-4996815.
STRINGi10090.ENSMUSP00000062677.

Structurei

3D structure databases

ProteinModelPortaliP51680.
SMRiP51680. Positions 11-325.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi343 – 3464Poly-Ser

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG148353.
GeneTreeiENSGT00760000118785.
HOGENOMiHOG000234122.
HOVERGENiHBG106917.
InParanoidiP51680.
KOiK04179.
OMAiKFRKYIV.
OrthoDBiEOG738051.
TreeFamiTF330966.

Family and domain databases

InterProiIPR002239. Chemokine_CCR4.
IPR000355. Chemokine_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24227. PTHR24227. 1 hit.
PTHR24227:SF12. PTHR24227:SF12. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00657. CCCHEMOKINER.
PR01109. CHEMOKINER4.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P51680-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNATEVTDTT QDETVYNSYY FYESMPKPCT KEGIKAFGEV FLPPLYSLVF
60 70 80 90 100
LLGLFGNSVV VLVLFKYKRL KSMTDVYLLN LAISDLLFVL SLPFWGYYAA
110 120 130 140 150
DQWVFGLGLC KIVSWMYLVG FYSGIFFIML MSIDRYLAIV HAVFSLKART
160 170 180 190 200
LTYGVITSLI TWSVAVFASL PGLLFSTCYT EHNHTYCKTQ YSVNSTTWKV
210 220 230 240 250
LSSLEINVLG LLIPLGIMLF CYSMIIRTLQ HCKNEKKNRA VRMIFAVVVL
260 270 280 290 300
FLGFWTPYNV VLFLETLVEL EVLQDCTLER YLDYAIQATE TLAFIHCCLN
310 320 330 340 350
PVIYFFLGEK FRKYITQLFR TCRGPLVLCK HCDFLQVYSA DMSSSSYTQS
360
TVDHDFRDAL
Length:360
Mass (Da):41,408
Last modified:July 27, 2011 - v2
Checksum:i8FA760951BE2002B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41T → I in AAA92582 (PubMed:9192769).Curated
Sequence conflicti145 – 1451S → T in AAA92582 (PubMed:9192769).Curated
Sequence conflicti181 – 1811E → Q in AAA92582 (PubMed:9192769).Curated
Sequence conflicti205 – 2051E → D in AAA92582 (PubMed:9192769).Curated
Sequence conflicti221 – 2211C → W in CAA62372 (PubMed:8573157).Curated
Sequence conflicti241 – 2411V → L in AAA92582 (PubMed:9192769).Curated
Sequence conflicti246 – 2461A → G in CAA62372 (PubMed:8573157).Curated
Sequence conflicti293 – 2931A → G in CAA62372 (PubMed:8573157).Curated
Sequence conflicti311 – 3111F → S in AAA92582 (PubMed:9192769).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X90862 mRNA. Translation: CAA62372.1.
U15208 mRNA. Translation: AAA92582.1.
CH466587 Genomic DNA. Translation: EDL09307.1.
CH466587 Genomic DNA. Translation: EDL09308.1.
BC117041 mRNA. Translation: AAI17042.1.
BC119171 mRNA. Translation: AAI19172.1.
CCDSiCCDS23594.1.
PIRiJC4587.
RefSeqiNP_034046.2. NM_009916.2.
XP_006511996.1. XM_006511933.2.
XP_006511997.1. XM_006511934.2.
XP_011241235.1. XM_011242933.1.
UniGeneiMm.1337.

Genome annotation databases

EnsembliENSMUST00000054414; ENSMUSP00000062677; ENSMUSG00000047898.
GeneIDi12773.
KEGGimmu:12773.
UCSCiuc009rxo.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X90862 mRNA. Translation: CAA62372.1.
U15208 mRNA. Translation: AAA92582.1.
CH466587 Genomic DNA. Translation: EDL09307.1.
CH466587 Genomic DNA. Translation: EDL09308.1.
BC117041 mRNA. Translation: AAI17042.1.
BC119171 mRNA. Translation: AAI19172.1.
CCDSiCCDS23594.1.
PIRiJC4587.
RefSeqiNP_034046.2. NM_009916.2.
XP_006511996.1. XM_006511933.2.
XP_006511997.1. XM_006511934.2.
XP_011241235.1. XM_011242933.1.
UniGeneiMm.1337.

3D structure databases

ProteinModelPortaliP51680.
SMRiP51680. Positions 11-325.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4996815.
STRINGi10090.ENSMUSP00000062677.

Chemistry

ChEMBLiCHEMBL5410.
GuidetoPHARMACOLOGYi61.

Protein family/group databases

GPCRDBiSearch...

Proteomic databases

PRIDEiP51680.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000054414; ENSMUSP00000062677; ENSMUSG00000047898.
GeneIDi12773.
KEGGimmu:12773.
UCSCiuc009rxo.2. mouse.

Organism-specific databases

CTDi1233.
MGIiMGI:107824. Ccr4.

Phylogenomic databases

eggNOGiNOG148353.
GeneTreeiENSGT00760000118785.
HOGENOMiHOG000234122.
HOVERGENiHBG106917.
InParanoidiP51680.
KOiK04179.
OMAiKFRKYIV.
OrthoDBiEOG738051.
TreeFamiTF330966.

Enzyme and pathway databases

ReactomeiREACT_288802. Chemokine receptors bind chemokines.
REACT_331048. G alpha (i) signalling events.

Miscellaneous databases

NextBioi282154.
PROiP51680.
SOURCEiSearch...

Gene expression databases

BgeeiP51680.
CleanExiMM_CCR4.
GenevisibleiP51680. MM.

Family and domain databases

InterProiIPR002239. Chemokine_CCR4.
IPR000355. Chemokine_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24227. PTHR24227. 1 hit.
PTHR24227:SF12. PTHR24227:SF12. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00657. CCCHEMOKINER.
PR01109. CHEMOKINER4.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of murine CC CKR-4 and high affinity binding of chemokines to murine and human CC CKR-4."
    Hoogewerf A.J., Black D., Proudfoot A.E.I., Wells T.N.C., Power C.A.
    Biochem. Biophys. Res. Commun. 218:337-343(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6 X CBA.
    Tissue: Thymus.
  2. "Molecular cloning and characterization of a cDNA, CHEMR1, encoding a chemokine receptor with a homology to the human C-C chemokine receptor, CCR-4."
    Youn B.-S., Kim S.-H., Lyu M.S., Kozak C.A., Taub D.D., Kwon B.S.
    Blood 89:4448-4460(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Cytotoxic T-cell.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Thymus.
  5. "A key role for CC chemokine receptor 4 in lipopolysaccharide-induced endotoxic shock."
    Buser R., Conquet F., Proudfoot A.E.I., Wells T.N.C., Power C.A.
    J. Exp. Med. 191:1755-1764(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiCCR4_MOUSE
AccessioniPrimary (citable) accession number: P51680
Secondary accession number(s): Q14A03
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 27, 2011
Last modified: July 22, 2015
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.