Reviewed, UniProtKB/Swiss-Prot P51680 (CCR4_MOUSE)

Names and origin

Protein names

Recommended name:
    C-C chemokine receptor type 4
      Short name=C-C CKR-4
      Short name=CC-CKR-4
      Short name=CCR-4
      Short name=CCR4
Alternative name(s):
    CD_antigen=CD194

Gene names

Name:	Ccr4
Synonyms:	Cmkbr4

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	High affinity receptor for the C-C type chemokines CCL17/TARC and CCL22/MDC. The activity of this receptor is mediated by G(i) proteins which activate a phosphatidylinositol-calcium second messenger system. Could play a role in lipopolysaccharide (LPS)-induced endotoxic shock. In the CNS, could mediate hippocampal-neuron survival. Ref.3
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Expressed in the thymus, macrophages and T- and B-cells.
Developmental stage	Low expression at 7.5 dpc and 12.5 dpc in the yolk sac.
Post-translational modification	In natural killer cells, CCL22 binding induces phosphorylation on yet undefined Ser/Thr residues, most probably by beta-adrenergic receptor kinases 1 and 2 By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW chemotaxis Ref.3 Inferred from mutant phenotype. Source: MGI inflammatory response Ref.3 Inferred from mutant phenotype. Source: MGI neuron migration Inferred from mutant phenotype. Source: MGI tolerance induction Inferred from mutant phenotype. Source: MGI
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	C-C chemokine receptor activity Ref.3 Inferred from mutant phenotype. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 360	360	C-C chemokine receptor type 4		PRO_0000069246
Regions
	Topological domain	1 – 39	39	Extracellular Potential
	Transmembrane	40 – 67	28	1 Potential
	Topological domain	68 – 77	10	Cytoplasmic Potential
	Transmembrane	78 – 98	21	2 Potential
	Topological domain	99 – 111	13	Extracellular Potential
	Transmembrane	112 – 133	22	3 Potential
	Topological domain	134 – 150	17	Cytoplasmic Potential
	Transmembrane	151 – 175	25	4 Potential
	Topological domain	176 – 206	31	Extracellular Potential
	Transmembrane	207 – 226	20	5 Potential
	Topological domain	227 – 242	16	Cytoplasmic Potential
	Transmembrane	243 – 267	25	6 Potential
	Topological domain	268 – 284	17	Extracellular Potential
	Transmembrane	285 – 308	24	7 Potential
	Topological domain	309 – 360	52	Cytoplasmic Potential
	Compositional bias	343 – 346	4	Poly-Ser
Amino acid modifications
	Glycosylation	2	1	N-linked (GlcNAc...) Potential
	Glycosylation	183	1	N-linked (GlcNAc...) Potential
	Glycosylation	194	1	N-linked (GlcNAc...) Potential
	Disulfide bond	110 ↔ 187		By similarity
Experimental info
	Sequence conflict	4	1	T → I in AAA92582. Ref.2
	Sequence conflict	145	1	S → T in AAA92582. Ref.2
	Sequence conflict	181	1	E → Q in AAA92582. Ref.2
	Sequence conflict	205	1	E → D in AAA92582. Ref.2
	Sequence conflict	221	1	W → C in AAA92582. Ref.2
	Sequence conflict	241	1	V → L in AAA92582. Ref.2
	Sequence conflict	246	1	G → A in AAA92582. Ref.2
	Sequence conflict	293	1	G → A in AAA92582. Ref.2
	Sequence conflict	311	1	F → S in AAA92582. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P51680-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 97BDB8C96D259AE3	FASTA	360	41,463
	10 20 30 40 50 60 MNATEVTDTT QDETVYNSYY FYESMPKPCT KEGIKAFGEV FLPPLYSLVF LLGLFGNSVV 70 80 90 100 110 120 VLVLFKYKRL KSMTDVYLLN LAISDLLFVL SLPFWGYYAA DQWVFGLGLC KIVSWMYLVG 130 140 150 160 170 180 FYSGIFFIML MSIDRYLAIV HAVFSLKART LTYGVITSLI TWSVAVFASL PGLLFSTCYT 190 200 210 220 230 240 EHNHTYCKTQ YSVNSTTWKV LSSLEINVLG LLIPLGIMLF WYSMIIRTLQ HCKNEKKNRA 250 260 270 280 290 300 VRMIFGVVVL FLGFWTPYNV VLFLETLVEL EVLQDCTLER YLDYAIQATE TLGFIHCCLN 310 320 330 340 350 360 PVIYFFLGEK FRKYITQLFR TCRGPLVLCK HCDFLQVYSA DMSSSSYTQS TVDHDFRDAL

References

[1]	"Molecular cloning of murine CC CKR-4 and high affinity binding of chemokines to murine and human CC CKR-4." Hoogewerf A.J., Black D., Proudfoot A.E.I., Wells T.N.C., Power C.A. Biochem. Biophys. Res. Commun. 218:337-343(1996) [PubMed: 8573157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6 X CBA. Tissue: Thymus.
[2]	"Molecular cloning and characterization of a cDNA, CHEMR1, encoding a chemokine receptor with a homology to the human C-C chemokine receptor, CCR-4." Youn B.-S., Kim S.-H., Lyu M.S., Kozak C.A., Taub D.D., Kwon B.S. Blood 89:4448-4460(1997) [PubMed: 9192769] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6. Tissue: Cytotoxic T-cell.
[3]	"A key role for CC chemokine receptor 4 in lipopolysaccharide-induced endotoxic shock." Buser R., Conquet F., Proudfoot A.E.I., Wells T.N.C., Power C.A. J. Exp. Med. 191:1755-1764(2000) [PubMed: 10811868] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X90862 mRNA. Translation: CAA62372.1. U15208 mRNA. Translation: AAA92582.1.
IPI	IPI00125251.
PIR	JC4587.
UniGene	Mm.1337
3D structure databases
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Genome annotation databases
Ensembl	ENSMUSG00000047898. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:107824. Ccr4.
Phylogenomic databases
HOGENOM	P51680.
HOVERGEN	P51680.
Gene expression databases
ArrayExpress	P51680.
Bgee	P51680.
CleanEx	MM_CCR4.
GermOnline	ENSMUSG00000047898. Mus musculus.
Family and domain databases
InterPro	IPR000276. 7TM_GPCR_Rhodpsn. IPR002239. CC_4_rcpt. IPR000355. Chmkine_rcpt. IPR017452. GPCR_Rhodpsn_supfam. [Graphical view]
PANTHER	PTHR19264:SF203. CC_4_rcpt. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00657. CCCHEMOKINER. PR01109. CHEMOKINER4. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

CCR4_MOUSE

Accession

Primary (citable) accession number: P51680

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)