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P51677 (CCR3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 136. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
C-C chemokine receptor type 3

Short name=C-C CKR-3
Short name=CC-CKR-3
Short name=CCR-3
Short name=CCR3
Short name=CKR3
Alternative name(s):
Eosinophil eotaxin receptor
CD_antigen=CD193
Gene names
Name:CCR3
Synonyms:CMKBR3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length355 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for a C-C type chemokine. Binds to eotaxin, eotaxin-3, MCP-3, MCP-4, RANTES and MIP-1 delta. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.

Subunit structure

Binds to HIV-1 Tat. Ref.13

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

In eosinophils as well as trace amounts in neutrophils and monocytes.

Miscellaneous

Overexpression of CCR3 together with its ligands appears to be a characteristic of ulcerative colitis (UC). The production of CCR3 ligands by human colonic epithelial cells suggests further that the epithelium can play a role in modulating pathological T-cell-mediated mucosal inflammation (Ref.14).

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
   Biological processHost-virus interaction
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processadenylate cyclase-modulating G-protein coupled receptor signaling pathway

Traceable author statement PubMed 10201960. Source: ProtInc

cell adhesion

Traceable author statement PubMed 10201960. Source: ProtInc

cellular defense response

Traceable author statement PubMed 10713092. Source: ProtInc

chemokine-mediated signaling pathway

Traceable author statement PubMed 10415069. Source: GOC

chemotaxis

Traceable author statement PubMed 10201960. Source: ProtInc

inflammatory response

Traceable author statement PubMed 9302298. Source: ProtInc

positive regulation of angiogenesis

Inferred from mutant phenotype PubMed 19525930. Source: BHF-UCL

positive regulation of cytosolic calcium ion concentration

Traceable author statement PubMed 9302298. Source: ProtInc

positive regulation of endothelial cell proliferation

Inferred from electronic annotation. Source: Ensembl

viral process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentintegral component of plasma membrane

Traceable author statement PubMed 9302298. Source: ProtInc

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functionC-C chemokine receptor activity

Inferred from electronic annotation. Source: Ensembl

chemokine receptor activity

Traceable author statement PubMed 10415069. Source: ProtInc

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CCL11P516712EBI-6625120,EBI-727357

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P51677-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P51677-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MPFGIRMLLRAHKPGSSRRSEM
Note: Gene prediction based on EST data.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 355355C-C chemokine receptor type 3
PRO_0000069239

Regions

Topological domain1 – 3434Extracellular Potential
Transmembrane35 – 6228Helical; Name=1; Potential
Topological domain63 – 7210Cytoplasmic Potential
Transmembrane73 – 9321Helical; Name=2; Potential
Topological domain94 – 10714Extracellular Potential
Transmembrane108 – 12922Helical; Name=3; Potential
Topological domain130 – 14617Cytoplasmic Potential
Transmembrane147 – 17125Helical; Name=4; Potential
Topological domain172 – 20332Extracellular Potential
Transmembrane204 – 22320Helical; Name=5; Potential
Topological domain224 – 23916Cytoplasmic Potential
Transmembrane240 – 26425Helical; Name=6; Potential
Topological domain265 – 28117Extracellular Potential
Transmembrane282 – 30524Helical; Name=7; Potential
Topological domain306 – 35550Cytoplasmic Potential

Amino acid modifications

Disulfide bond106 ↔ 183 By similarity

Natural variations

Alternative sequence11M → MPFGIRMLLRAHKPGSSRRS EM in isoform 2.
VSP_046704
Natural variant211G → D.
Corresponds to variant rs4987125 [ dbSNP | Ensembl ].
VAR_020067
Natural variant391P → L.
Corresponds to variant rs5742906 [ dbSNP | Ensembl ].
VAR_049382
Natural variant2181C → S Polymorphism found in about 7% of the population; may show reduced activity. Ref.6
VAR_010668

Experimental info

Sequence conflict81 – 822LL → QG in AAL85630. Ref.7
Sequence conflict2051I → V in BAG53863. Ref.10
Sequence conflict2761S → T in AAB09726. Ref.4
Sequence conflict2761S → T in BAA86964. Ref.6
Sequence conflict2771K → R in BAG53863. Ref.10
Sequence conflict3491P → R in BAG53863. Ref.10

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: E95DCD7A6C643874

FASTA35541,044
        10         20         30         40         50         60 
MTTSLDTVET FGTTSYYDDV GLLCEKADTR ALMAQFVPPL YSLVFTVGLL GNVVVVMILI 

        70         80         90        100        110        120 
KYRRLRIMTN IYLLNLAISD LLFLVTLPFW IHYVRGHNWV FGHGMCKLLS GFYHTGLYSE 

       130        140        150        160        170        180 
IFFIILLTID RYLAIVHAVF ALRARTVTFG VITSIVTWGL AVLAALPEFI FYETEELFEE 

       190        200        210        220        230        240 
TLCSALYPED TVYSWRHFHT LRMTIFCLVL PLLVMAICYT GIIKTLLRCP SKKKYKAIRL 

       250        260        270        280        290        300 
IFVIMAVFFI FWTPYNVAIL LSSYQSILFG NDCERSKHLD LVMLVTEVIA YSHCCMNPVI 

       310        320        330        340        350 
YAFVGERFRK YLRHFFHRHL LMHLGRYIPF LPSEKLERTS SVSPSTAEPE LSIVF 

« Hide

Isoform 2 [UniParc].

Checksum: 422D094FF3497FE4
Show »

FASTA37643,453

References

« Hide 'large scale' references
[1]"Cloning and functional expression of a human eosinophil CC chemokine receptor."
Combadiere C., Ahuja S.K., Murphy P.M.
J. Biol. Chem. 270:16491-16494(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Monocyte.
[2]Erratum
Combadiere C., Ahuja S.K., Murphy P.M.
J. Biol. Chem. 270:30235-30235(1995)
[3]"Cloning, expression, and characterization of the human eosinophil eotaxin receptor."
Daugherty B.L., Siciliano S.J., Demartino J.A., Malkowitz L., Sirotina A., Springer M.S.
J. Exp. Med. 183:2349-2354(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
[4]"Molecular cloning and characterization of a human eotaxin receptor expressed selectively on eosinophils."
Ponath P.D., Qin S., Post T.W., Wang J., Wu L., Gerard N.P., Newman W., Gerard C., Mackay C.R.
J. Exp. Med. 183:2437-2448(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
[5]"Partial resistance to infection by syncytium-inducing primary HIV-1 in exposed uninfected individuals homozygous for CCR5 32bp deletion."
Xiao L., Weiss S., Qari S., Rudolph D., Hodge T., Lal R.
Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[6]"New variations of human CC-chemokine receptors CCR3 and CCR4."
Kato H., Tsuchiya N., Izumi S., Miyamasu M., Nakajima T., Kawasaki H., Hirai K., Tokunaga K.
Genes Immun. 1:97-104(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1), VARIANT SER-218.
[7]"Transcription regulation of human chemokine receptor CCR3: evidence for a rare TATA-less promoter structure conserved between Drosophila and humans."
Vijh S., Dayhoff D.E., Wang C.E., Imam Z., Ehrenberg P.K., Michael N.L.
Genomics 80:86-95(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
[8]"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
Kopatz S.A., Aronstam R.S., Sharma S.V.
Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
[9]Livingston R.J., Shaffer T., McFarland I., Nguyen C.P., Stanaway I.B., Rajkumar N., Johnson E.J., da Ponte S.H., Willa H., Ahearn M.O., Bertucci C., Acklestad J., Carroll A., Swanson J., Gildersleeve H.I., Nickerson D.A.
Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
[10]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Synovium.
[11]"The DNA sequence, annotation and analysis of human chromosome 3."
Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. expand/collapse author list , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[12]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Blood.
[13]"HIV-1 Tat protein mimicry of chemokines."
Albini A., Ferrini S., Benelli R., Sforzini S., Giunciuglio D., Aluigi M.G., Proudfoot A.E.I., Alouani S., Wells T.N.C., Mariani G., Rabin R.L., Farber J.M., Noonan D.M.
Proc. Natl. Acad. Sci. U.S.A. 95:13153-13158(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HIV-1 TAT.
[14]"Increased expression of chemokine receptor CCR3 and its ligands in ulcerative colitis: the role of colonic epithelial cells in in vitro studies."
Manousou P., Kolios G., Valatas V., Drygiannakis I., Bourikas L., Pyrovolaki K., Koutroubakis I., Papadaki H.A., Kouroumalis E.
Clin. Exp. Immunol. 162:337-347(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: OVEREXPRESSION.
+Additional computationally mapped references.

Web resources

Wikipedia

CC chemokine receptors entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U28694 mRNA. Translation: AAC50469.1.
U51241 Genomic DNA. Translation: AAB16831.1.
U49727 Genomic DNA. Translation: AAB09726.1.
AF026535 mRNA. Translation: AAB82589.1.
AB023887 Genomic DNA. Translation: BAA86964.1.
AF262301 mRNA. Translation: AAL85630.1.
AF247361 Genomic DNA. Translation: AAL85154.1.
AY221092 Genomic DNA. Translation: AAO65970.2.
EF064760 Genomic DNA. Translation: ABK41943.1.
AK123050 mRNA. Translation: BAG53863.1.
AC104439 Genomic DNA. No translation available.
BC110297 mRNA. Translation: AAI10298.1.
BC130318 mRNA. Translation: AAI30319.1.
BC130320 mRNA. Translation: AAI30321.1.
PIRG02436.
RefSeqNP_001828.1. NM_001837.3.
NP_847898.1. NM_178328.1.
NP_847899.1. NM_178329.2.
UniGeneHs.506190.

3D structure databases

ProteinModelPortalP51677.
SMRP51677. Positions 24-317.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107637. 7 interactions.
DIPDIP-5846N.
IntActP51677. 1 interaction.
MINTMINT-105239.
STRING9606.ENSP00000350003.

Chemistry

BindingDBP51677.
ChEMBLCHEMBL3473.
GuidetoPHARMACOLOGY60.

Protein family/group databases

GPCRDBSearch...

Polymorphism databases

DMDM1705892.

Proteomic databases

PaxDbP51677.
PRIDEP51677.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000357422; ENSP00000350003; ENSG00000183625. [P51677-1]
ENST00000395940; ENSP00000379271; ENSG00000183625. [P51677-1]
ENST00000395942; ENSP00000379273; ENSG00000183625. [P51677-1]
ENST00000541018; ENSP00000440097; ENSG00000183625. [P51677-1]
ENST00000545097; ENSP00000441600; ENSG00000183625. [P51677-2]
GeneID1232.
KEGGhsa:1232.
UCSCuc003cpg.2. human. [P51677-1]

Organism-specific databases

CTD1232.
GeneCardsGC03P046227.
HGNCHGNC:1604. CCR3.
HPACAB003795.
MIM601268. gene.
neXtProtNX_P51677.
PharmGKBPA26168.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG148353.
HOGENOMHOG000234122.
HOVERGENHBG106917.
InParanoidP51677.
KOK04178.
OMAYIPFLPS.
OrthoDBEOG738051.
PhylomeDBP51677.
TreeFamTF330966.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

ArrayExpressP51677.
BgeeP51677.
CleanExHS_CCR3.
GenevestigatorP51677.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR002238. Chemokine_CCR3.
IPR000355. Chemokine_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERPTHR24227. PTHR24227. 1 hit.
PTHR24227:SF13. PTHR24227:SF13. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00657. CCCHEMOKINER.
PR01108. CHEMOKINER3.
PR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi1232.
NextBio5025.
PROP51677.
SOURCESearch...

Entry information

Entry nameCCR3_HUMAN
AccessionPrimary (citable) accession number: P51677
Secondary accession number(s): B3KVQ1 expand/collapse secondary AC list , F5GWL6, Q15748, Q2YDB9, Q86WD2, Q8TDP6, Q9ULY8
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries