P51671 (CCL11_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Eotaxin

Alternative name(s):
C-C motif chemokine 11
Eosinophil chemotactic protein
Small-inducible cytokine A11

Gene names

Name:	CCL11
Synonyms:	SCYA11

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	97 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3.
Subcellular location	Secreted.
Induction	By TNF, IL1A/interleukin-1 alpha and IFNG/IFN-gamma.
Post-translational modification	O-linked glycan consists of a Gal-GalNAc disaccharide which is mofified with up to 2 sialic acid residues.
Sequence similarities	Belongs to the intercrine beta (chemokine CC) family.

Ontologies

Keywords
Biological process	Chemotaxis Inflammatory response
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Cytokine
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	ERK1 and ERK2 cascade Inferred from electronic annotation. Source: Compara actin filament organization Inferred from electronic annotation. Source: Compara branching involved in mammary gland duct morphogenesis Inferred from electronic annotation. Source: Compara cell adhesion Traceable author statement PubMed 10201960. Source: ProtInc cellular calcium ion homeostasis Traceable author statement PubMed 10415069. Source: ProtInc chronic inflammatory response Inferred from electronic annotation. Source: Compara cytoskeleton organization Inferred from direct assay PubMed 10072545. Source: UniProtKB eosinophil chemotaxis Inferred from direct assay PubMed 10072545. Source: UniProtKB immune response Inferred from electronic annotation. Source: InterPro inflammatory response Traceable author statement PubMed 10708591. Source: ProtInc mammary duct terminal end bud growth Inferred from electronic annotation. Source: Compara mast cell chemotaxis Inferred from electronic annotation. Source: Compara positive regulation of Rac GTPase activity Inferred from direct assay PubMed 19525930. Source: BHF-UCL positive regulation of actin filament polymerization Inferred from direct assay PubMed 19525930. Source: BHF-UCL positive regulation of angiogenesis Inferred from electronic annotation. Source: Compara positive regulation of cell migration Inferred from direct assay PubMed 19525930. Source: BHF-UCL positive regulation of endothelial cell proliferation Inferred from direct assay PubMed 19525930. Source: BHF-UCL protein phosphorylation Traceable author statement PubMed 10706854. Source: ProtInc regulation of cell shape Inferred from direct assay PubMed 10072545. Source: UniProtKB response to interleukin-4 Inferred from electronic annotation. Source: Compara response to radiation Traceable author statement PubMed 10708591. Source: ProtInc response to virus Traceable author statement PubMed 9558100. Source: ProtInc signal transduction Traceable author statement PubMed 10706854. Source: ProtInc
Cellular_component	extracellular region Inferred from direct assay PubMed 20041150. Source: BHF-UCL extracellular space Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	chemokine activity Inferred from direct assay PubMed 10072545. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 23

23

Chain

24 – 97

74

Eotaxin

PRO_0000005195

Amino acid modifications

Glycosylation

94

1

O-linked (GalNAc...) Ref.8

Disulfide bond

32 ↔ 57

Disulfide bond

33 ↔ 73

Natural variations

Natural variant

7

1

L → P in clone 34.

VAR_001634

Natural variant

23

1

A → T in clone 53.
Corresponds to variant rs1129844 [ dbSNP | Ensembl ].

VAR_001635

Natural variant

51

1

R → S in clone 34.

VAR_001636

Natural variant

79

1

K → R in clone 53.

VAR_001637

Natural variant

86

1

K → T.
Corresponds to variant rs34262946 [ dbSNP | Ensembl ].

VAR_048705

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

97

Helix Strand Turn

Details...

Beta strand

26 – 29

4

Beta strand

46 – 52

7

Beta strand

55 – 59

5

Beta strand

61 – 66

6

Beta strand

71 – 74

4

Helix

79 – 91

13

Sequences

	Sequence		Length	Mass (Da)
	P51671 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: B433C30FDA4C71A7	FASTA	97	10,732
	10 20 30 40 50 60 MKVSAALLWL LLIAAAFSPQ GLAGPASVPT TCCFNLANRK IPLQRLESYR RITSGKCPQK 70 80 90 AVIFKTKLAK DICADPKKKW VQDSMKYLDQ KSPTPKP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human eotaxin is a specific chemoattractant for eosinophil cells and provides a new mechanism to explain tissue eosinophilia." Garcia-Zepeda E.A., Rothenberg M.E., Ownbey T.R., Leder P., Luster A.D. Nat. Med. 2:449-456(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning of the human eosinophil chemoattractant, eotaxin. Expression, receptor binding, and functional properties suggest a mechanism for the selective recruitment of eosinophils." Ponath P.D., Qin S., Ringler D.J., Clark-Lewis I., Wang J., Kassam N., Smith H., Shi X., Gonzalo J.A., Newman W., Gutierrez-Ramos J.-C., Mackay C.R. J. Clin. Invest. 97:604-612(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Molecular cloning of human eotaxin, an eosinophil-selective CC chemokine, and identification of a specific eosinophil eotaxin receptor, CC chemokine receptor 3." Kitaura M., Nakajima T., Imai T., Harada S., Combadiere C., Tiffany H.L., Murphy P.M., Yoshie O. J. Biol. Chem. 271:7725-7730(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Small intestine.
[4]	"Human dermal fibroblasts express eotaxin: molecular cloning, mRNA expression, and identification of eotaxin sequence variants." Bartels J., Schlueter C., Richter E., Noso N., Kulke R., Christophers E., Schroeder J.-M. Biochem. Biophys. Res. Commun. 225:1045-1051(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 60-65 AND 75-88, VARIANTS. Tissue: Foreskin.
[5]	"Genomic organization, complete sequence, and chromosomal location of the gene for human eotaxin (SCYA11), an eosinophil-specific CC chemokine." Garcia-Zepeda E.A., Rothenberg M.E., Weremowicz S., Sarafi M.N., Morton C.C., Luster A.D. Genomics 41:471-476(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Placenta.
[6]	"Genomic organization, sequence, and transcriptional regulation of the human eotaxin gene." Hein H., Schlueter C., Kulke R., Christophers E., Schroeder J.-M., Bartels J. Biochem. Biophys. Res. Commun. 237:537-542(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Lung.
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[8]	"Delayed production of biologically active O-glycosylated forms of human eotaxin by tumor-necrosis-factor-alpha-stimulated dermal fibroblasts." Noso N., Bartels J., Mallet A.I., Mochizuki M., Christophers E., Schroeder J.-M. Eur. J. Biochem. 253:114-122(1998) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION AT THR-94. Tissue: Blood.
[9]	"Solution structure of eotaxin, a chemokine that selectively recruits eosinophils in allergic inflammation." Crump M.P., Rajarathnam K., Kim K.S., Clark-Lewis I., Sykes B.D. J. Biol. Chem. 273:22471-22479(1998) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Web resources

R&D Systems' cytokine source book: CCL11

Wikipedia

CCL11 entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U46573 mRNA. Translation: AAA98957.1.
U34780 Genomic DNA. Translation: AAC50369.1.
D49372 mRNA. Translation: BAA08370.1.
Z69291 mRNA. Translation: CAA93258.1.
Z75668 mRNA. Translation: CAA99997.1.
Z75669 mRNA. Translation: CAA99998.1.
U46572 Genomic DNA. Translation: AAC51297.1.
Z92709 Genomic DNA. Translation: CAB07027.1.
BC017850 mRNA. Translation: AAH17850.1.

IPI

IPI00019945.

PIR

JC4912.

RefSeq

NP_002977.1. NM_002986.2.

UniGene

Hs.54460.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EOT	NMR	-	A	24-97	[»]
2EOT	NMR	-	A	24-97	[»]

ProteinModelPortal

P51671.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-5858N.

IntAct

P51671. 1 interaction.

MINT

MINT-103325.

STRING

9606.ENSP00000302234.

PTM databases

PhosphoSite

P51671.

Polymorphism databases

DMDM

1706661.

Proteomic databases

PaxDb

P51671.

PRIDE

P51671.

Protocols and materials databases

DNASU

6356.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000305869; ENSP00000302234; ENSG00000172156.

GeneID

6356.

KEGG

hsa:6356.

UCSC

uc002hia.1. human.

Organism-specific databases

CTD

6356.

GeneCards

GC17P032612.

HGNC

HGNC:10610. CCL11.

MIM

601156. gene.

neXtProt

NX_P51671.

PharmGKB

PA35543.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG46290.

HOGENOM

HOG000036686.

HOVERGEN

HBG017871.

InParanoid

P51671.

KO

K16597.

OMA

GSKCPQK.

OrthoDB

EOG45DWR1.

PhylomeDB

P51671.

Enzyme and pathway databases

Pathway_Interaction_DB

il4_2pathway. IL4-mediated signaling events.

Reactome

REACT_111102. Signal Transduction.

Gene expression databases

ArrayExpress

P51671.

Bgee

P51671.

CleanEx

HS_CCL11.

Genevestigator

P51671.

GermOnline

ENSG00000172156. Homo sapiens.

Family and domain databases

InterPro

IPR000827. Chemokine_CC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]

Pfam

PF00048. IL8. 1 hit.
[Graphical view]

SMART

SM00199. SCY. 1 hit.
[Graphical view]

SUPFAM

SSF54117. Chemokine_IL8. 1 hit.

PROSITE

PS00472. SMALL_CYTOKINES_CC. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P51671.

GenomeRNAi

6356.

NextBio

24688.

PMAP-CutDB

P51671.

SOURCE

Search...

Entry information

Entry name

CCL11_HUMAN

Accession

Primary (citable) accession number: P51671
Secondary accession number(s): P50877, Q92490, Q92491

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 131 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.