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P51667

- MLRV_MOUSE

UniProt

P51667 - MLRV_MOUSE

Protein

Myosin regulatory light chain 2, ventricular/cardiac muscle isoform

Gene

Myl2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 3 (03 Apr 2007)
      Previous versions | rss
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    Functioni

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi37 – 4812Add
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: MGI

    GO - Biological processi

    1. cardiac muscle contraction Source: MGI
    2. cardiac myofibril assembly Source: MGI
    3. heart contraction Source: MGI
    4. heart development Source: MGI
    5. heart morphogenesis Source: MGI
    6. muscle cell fate specification Source: MGI
    7. muscle fiber development Source: MGI
    8. negative regulation of cell growth Source: Ensembl
    9. post-embryonic development Source: MGI
    10. ventricular cardiac muscle tissue morphogenesis Source: Ensembl

    Keywords - Molecular functioni

    Motor protein, Muscle protein, Myosin

    Keywords - Ligandi

    Calcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Myosin regulatory light chain 2, ventricular/cardiac muscle isoform
    Short name:
    MLC-2
    Short name:
    MLC-2v
    Gene namesi
    Name:Myl2
    Synonyms:Mylpc
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:97272. Myl2.

    Subcellular locationi

    CytoplasmmyofibrilsarcomereA band By similarity

    GO - Cellular componenti

    1. A band Source: UniProtKB-SubCell
    2. myofibril Source: MGI
    3. myosin complex Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 166165Myosin regulatory light chain 2, ventricular/cardiac muscle isoformPRO_0000198728Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N,N,N-trimethylalanine1 Publication

    Post-translational modificationi

    N-terminus is methylated by METTL11A/NTM1.1 Publication
    Phosphorylated by MYLK3.By similarity

    Keywords - PTMi

    Methylation, Phosphoprotein

    Proteomic databases

    MaxQBiP51667.
    PaxDbiP51667.
    PRIDEiP51667.

    PTM databases

    PhosphoSiteiP51667.

    Expressioni

    Gene expression databases

    BgeeiP51667.
    GenevestigatoriP51667.

    Interactioni

    Subunit structurei

    Myosin is a hexamer of 2 heavy chains and 4 light chains. Interacts with MYOC By similarity.By similarity

    Protein-protein interaction databases

    IntActiP51667. 3 interactions.
    MINTiMINT-4130491.

    Structurei

    3D structure databases

    ProteinModelPortaliP51667.
    SMRiP51667. Positions 16-165.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini24 – 5936EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini94 – 12936EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini130 – 16536EF-hand 3PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 3 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5126.
    GeneTreeiENSGT00730000110764.
    HOGENOMiHOG000233018.
    HOVERGENiHBG012180.
    KOiK10351.
    OMAiTQMLTTQ.
    OrthoDBiEOG7992RX.
    PhylomeDBiP51667.
    TreeFamiTF314218.

    Family and domain databases

    Gene3Di1.10.238.10. 2 hits.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    [Graphical view]
    PfamiPF00036. EF-hand_1. 1 hit.
    [Graphical view]
    SMARTiSM00054. EFh. 3 hits.
    [Graphical view]
    PROSITEiPS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P51667-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAPKKAKKRI EGGSSNVFSM FEQTQIQEFK EAFTIMDQNR DGFIDKNDLR    50
    DTFAALGRVN VKNEEIDEMI KEAPGPINFT VFLTMFGEKL KGADPEETIL 100
    NAFKVFDPEG KGSLKADYVR EMLTTQAERF SKEEIDQMFA AFPPDVTGNL 150
    DYKNLVHIIT HGEEKD 166
    Length:166
    Mass (Da):18,864
    Last modified:April 3, 2007 - v3
    Checksum:iEA25C1FAA18E2EA8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti4 – 52KK → LF in AAA39796. (PubMed:1379240)Curated
    Sequence conflicti14 – 141S → T in AAA39796. (PubMed:1379240)Curated
    Sequence conflicti128 – 1281E → G in AAH61144. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M91602 mRNA. Translation: AAA39796.1.
    AK002367 mRNA. Translation: BAB22045.1.
    AK146674 mRNA. Translation: BAE27350.1.
    BC061144 mRNA. Translation: AAH61144.1.
    CCDSiCCDS39252.1.
    PIRiA42858.
    RefSeqiNP_034991.3. NM_010861.3.
    UniGeneiMm.1529.

    Genome annotation databases

    EnsembliENSMUST00000014080; ENSMUSP00000014080; ENSMUSG00000013936.
    ENSMUST00000111751; ENSMUSP00000107380; ENSMUSG00000013936.
    GeneIDi17906.
    KEGGimmu:17906.
    UCSCiuc008zkp.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M91602 mRNA. Translation: AAA39796.1 .
    AK002367 mRNA. Translation: BAB22045.1 .
    AK146674 mRNA. Translation: BAE27350.1 .
    BC061144 mRNA. Translation: AAH61144.1 .
    CCDSi CCDS39252.1.
    PIRi A42858.
    RefSeqi NP_034991.3. NM_010861.3.
    UniGenei Mm.1529.

    3D structure databases

    ProteinModelPortali P51667.
    SMRi P51667. Positions 16-165.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P51667. 3 interactions.
    MINTi MINT-4130491.

    PTM databases

    PhosphoSitei P51667.

    Proteomic databases

    MaxQBi P51667.
    PaxDbi P51667.
    PRIDEi P51667.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000014080 ; ENSMUSP00000014080 ; ENSMUSG00000013936 .
    ENSMUST00000111751 ; ENSMUSP00000107380 ; ENSMUSG00000013936 .
    GeneIDi 17906.
    KEGGi mmu:17906.
    UCSCi uc008zkp.1. mouse.

    Organism-specific databases

    CTDi 4633.
    MGIi MGI:97272. Myl2.

    Phylogenomic databases

    eggNOGi COG5126.
    GeneTreei ENSGT00730000110764.
    HOGENOMi HOG000233018.
    HOVERGENi HBG012180.
    KOi K10351.
    OMAi TQMLTTQ.
    OrthoDBi EOG7992RX.
    PhylomeDBi P51667.
    TreeFami TF314218.

    Miscellaneous databases

    ChiTaRSi MYL2. mouse.
    NextBioi 292733.
    PROi P51667.
    SOURCEi Search...

    Gene expression databases

    Bgeei P51667.
    Genevestigatori P51667.

    Family and domain databases

    Gene3Di 1.10.238.10. 2 hits.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    [Graphical view ]
    Pfami PF00036. EF-hand_1. 1 hit.
    [Graphical view ]
    SMARTi SM00054. EFh. 3 hits.
    [Graphical view ]
    PROSITEi PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Myosin light chain-2 luciferase transgenic mice reveal distinct regulatory programs for cardiac and skeletal muscle-specific expression of a single contractile protein gene."
      Lee K.J., Ross R.S., Rockman H.A., Harris A.N., O'Brien T.X., Bilsen M., Shubeita H.E., Kandolf R., Brem G., Price J., Evans S.M., Zhu H., Franz W.M., Chien K.R.
      J. Biol. Chem. 267:15875-15885(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Heart muscle.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Embryonic heart and Kidney.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. "NRMT is an alpha-N-methyltransferase that methylates RCC1 and retinoblastoma protein."
      Tooley C.E., Petkowski J.J., Muratore-Schroeder T.L., Balsbaugh J.L., Shabanowitz J., Sabat M., Minor W., Hunt D.F., Macara I.G.
      Nature 466:1125-1128(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: CLEAVAGE OF INITIATOR METHIONINE, METHYLATION AT ALA-2.

    Entry informationi

    Entry nameiMLRV_MOUSE
    AccessioniPrimary (citable) accession number: P51667
    Secondary accession number(s): Q6P8P4, Q9QVP3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: April 3, 2007
    Last modified: October 1, 2014
    This is version 119 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This chain binds calcium.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3