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P51667 (MLRV_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Myosin regulatory light chain 2, ventricular/cardiac muscle isoform

Short name=MLC-2
Short name=MLC-2v
Gene names
Name:Myl2
Synonyms:Mylpc
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length166 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Myosin is a hexamer of 2 heavy chains and 4 light chains. Interacts with MYOC By similarity.

Subcellular location

CytoplasmmyofibrilsarcomereA band By similarity.

Post-translational modification

N-terminus is methylated by METTL11A/NTM1. Ref.4

Phosphorylated by MYLK3 By similarity.

Miscellaneous

This chain binds calcium.

Sequence similarities

Contains 3 EF-hand domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.4
Chain2 – 166165Myosin regulatory light chain 2, ventricular/cardiac muscle isoform
PRO_0000198728

Regions

Domain24 – 5936EF-hand 1
Domain94 – 12936EF-hand 2
Domain130 – 16536EF-hand 3
Calcium binding37 – 4812

Amino acid modifications

Modified residue21N,N,N-trimethylalanine Ref.4

Experimental info

Sequence conflict4 – 52KK → LF in AAA39796. Ref.1
Sequence conflict141S → T in AAA39796. Ref.1
Sequence conflict1281E → G in AAH61144. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P51667 [UniParc].

Last modified April 3, 2007. Version 3.
Checksum: EA25C1FAA18E2EA8

FASTA16618,864
        10         20         30         40         50         60 
MAPKKAKKRI EGGSSNVFSM FEQTQIQEFK EAFTIMDQNR DGFIDKNDLR DTFAALGRVN 

        70         80         90        100        110        120 
VKNEEIDEMI KEAPGPINFT VFLTMFGEKL KGADPEETIL NAFKVFDPEG KGSLKADYVR 

       130        140        150        160 
EMLTTQAERF SKEEIDQMFA AFPPDVTGNL DYKNLVHIIT HGEEKD 

« Hide

References

« Hide 'large scale' references
[1]"Myosin light chain-2 luciferase transgenic mice reveal distinct regulatory programs for cardiac and skeletal muscle-specific expression of a single contractile protein gene."
Lee K.J., Ross R.S., Rockman H.A., Harris A.N., O'Brien T.X., Bilsen M., Shubeita H.E., Kandolf R., Brem G., Price J., Evans S.M., Zhu H., Franz W.M., Chien K.R.
J. Biol. Chem. 267:15875-15885(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Heart muscle.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Embryonic heart and Kidney.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"NRMT is an alpha-N-methyltransferase that methylates RCC1 and retinoblastoma protein."
Tooley C.E., Petkowski J.J., Muratore-Schroeder T.L., Balsbaugh J.L., Shabanowitz J., Sabat M., Minor W., Hunt D.F., Macara I.G.
Nature 466:1125-1128(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: CLEAVAGE OF INITIATOR METHIONINE, METHYLATION AT ALA-2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M91602 mRNA. Translation: AAA39796.1.
AK002367 mRNA. Translation: BAB22045.1.
AK146674 mRNA. Translation: BAE27350.1.
BC061144 mRNA. Translation: AAH61144.1.
CCDSCCDS39252.1.
PIRA42858.
RefSeqNP_034991.3. NM_010861.3.
UniGeneMm.1529.

3D structure databases

ProteinModelPortalP51667.
SMRP51667. Positions 16-165.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP51667. 3 interactions.
MINTMINT-4130491.

PTM databases

PhosphoSiteP51667.

Proteomic databases

MaxQBP51667.
PaxDbP51667.
PRIDEP51667.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000014080; ENSMUSP00000014080; ENSMUSG00000013936.
ENSMUST00000111751; ENSMUSP00000107380; ENSMUSG00000013936.
GeneID17906.
KEGGmmu:17906.
UCSCuc008zkp.1. mouse.

Organism-specific databases

CTD4633.
MGIMGI:97272. Myl2.

Phylogenomic databases

eggNOGCOG5126.
GeneTreeENSGT00730000110764.
HOGENOMHOG000233018.
HOVERGENHBG012180.
KOK10351.
OMATQMLTTQ.
OrthoDBEOG7992RX.
PhylomeDBP51667.
TreeFamTF314218.

Gene expression databases

BgeeP51667.
GenevestigatorP51667.

Family and domain databases

Gene3D1.10.238.10. 2 hits.
InterProIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamPF00036. EF-hand_1. 1 hit.
[Graphical view]
SMARTSM00054. EFh. 3 hits.
[Graphical view]
PROSITEPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSMYL2. mouse.
NextBio292733.
PROP51667.
SOURCESearch...

Entry information

Entry nameMLRV_MOUSE
AccessionPrimary (citable) accession number: P51667
Secondary accession number(s): Q6P8P4, Q9QVP3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: April 3, 2007
Last modified: July 9, 2014
This is version 118 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot