P51667 (MLRV_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Myosin regulatory light chain 2, ventricular/cardiac muscle isoform
Short name=MLC-2
Short name=MLC-2v

Gene names

Name:	Myl2
Synonyms:	Mylpc

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	166 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Myosin is a hexamer of 2 heavy chains and 4 light chains.
Subcellular location	Cytoplasm › myofibril › sarcomere › A band By similarity.
Post-translational modification	N-terminus is methylated by METTL11A/NTM1. Ref.4 Phosphorylated by MYLK3 By similarity.
Miscellaneous	This chain binds calcium.
Sequence similarities	Contains 3 EF-hand domains.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Repeat
Ligand	Calcium Metal-binding
Molecular function	Motor protein Muscle protein Myosin
PTM	Methylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cardiac muscle contraction Inferred from genetic interaction PubMed 15308581. Source: MGI cardiac myofibril assembly Inferred from mutant phenotype PubMed 9422794. Source: MGI heart morphogenesis Inferred from genetic interaction PubMed 15308581. Source: MGI muscle cell fate specification Inferred from mutant phenotype Ref.1. Source: MGI muscle fiber development Inferred from genetic interaction PubMed 15308581. Source: MGI negative regulation of cell growth Inferred from electronic annotation. Source: Compara post-embryonic development Inferred from genetic interaction PubMed 15308581. Source: MGI ventricular cardiac muscle tissue morphogenesis Inferred from electronic annotation. Source: Compara
Cellular_component	myofibril Inferred from direct assay PubMed 11356630. Source: MGI myosin complex Inferred from electronic annotation. Source: UniProtKB-KW sarcomere Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium ion binding Inferred from sequence orthology PubMed 11102452. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4
	Chain	2 – 166	165	Myosin regulatory light chain 2, ventricular/cardiac muscle isoform		PRO_0000198728
Regions
	Domain	24 – 59	36	EF-hand 1
	Domain	94 – 129	36	EF-hand 2
	Domain	130 – 165	36	EF-hand 3
	Calcium binding	37 – 48	12
Amino acid modifications
	Modified residue	2	1	N,N,N-trimethylalanine Ref.4
Experimental info
	Sequence conflict	4 – 5	2	KK → LF in AAA39796. Ref.1
	Sequence conflict	14	1	S → T in AAA39796. Ref.1
	Sequence conflict	128	1	E → G in AAH61144. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P51667 [UniParc]. Last modified April 3, 2007. Version 3. Checksum: EA25C1FAA18E2EA8	FASTA	166	18,864
	10 20 30 40 50 60 MAPKKAKKRI EGGSSNVFSM FEQTQIQEFK EAFTIMDQNR DGFIDKNDLR DTFAALGRVN 70 80 90 100 110 120 VKNEEIDEMI KEAPGPINFT VFLTMFGEKL KGADPEETIL NAFKVFDPEG KGSLKADYVR 130 140 150 160 EMLTTQAERF SKEEIDQMFA AFPPDVTGNL DYKNLVHIIT HGEEKD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Myosin light chain-2 luciferase transgenic mice reveal distinct regulatory programs for cardiac and skeletal muscle-specific expression of a single contractile protein gene." Lee K.J., Ross R.S., Rockman H.A., Harris A.N., O'Brien T.X., Bilsen M., Shubeita H.E., Kandolf R., Brem G., Price J., Evans S.M., Zhu H., Franz W.M., Chien K.R. J. Biol. Chem. 267:15875-15885(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Heart muscle.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Embryonic heart and Kidney.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"NRMT is an alpha-N-methyltransferase that methylates RCC1 and retinoblastoma protein." Tooley C.E., Petkowski J.J., Muratore-Schroeder T.L., Balsbaugh J.L., Shabanowitz J., Sabat M., Minor W., Hunt D.F., Macara I.G. Nature 466:1125-1128(2010) [PubMed] [Europe PMC] [Abstract] Cited for: CLEAVAGE OF INITIATOR METHIONINE, METHYLATION AT ALA-2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M91602 mRNA. Translation: AAA39796.1. AK002367 mRNA. Translation: BAB22045.1. AK146674 mRNA. Translation: BAE27350.1. BC061144 mRNA. Translation: AAH61144.1.
IPI	IPI00555015.
PIR	A42858.
RefSeq	NP_034991.3. NM_010861.3.
UniGene	Mm.1529.
3D structure databases
ProteinModelPortal	P51667.
SMR	P51667. Positions 16-165.
ModBase	Search...
Protein-protein interaction databases
IntAct	P51667. 1 interaction.
PTM databases
PhosphoSite	P51667.
Proteomic databases
PaxDb	P51667.
PRIDE	P51667.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000014080; ENSMUSP00000014080; ENSMUSG00000013936. ENSMUST00000111751; ENSMUSP00000107380; ENSMUSG00000013936.
GeneID	17906.
KEGG	mmu:17906.
UCSC	uc008zkp.1. mouse.
Organism-specific databases
CTD	4633.
MGI	MGI:97272. Myl2.
Phylogenomic databases
eggNOG	COG5126.
GeneTree	ENSGT00590000082808.
HOGENOM	HOG000233018.
HOVERGEN	HBG012180.
KO	K10351.
OMA	MTQGERF.
OrthoDB	EOG4JT06P.
Gene expression databases
ArrayExpress	P51667.
Bgee	P51667.
Genevestigator	P51667.
GermOnline	ENSMUSG00000013936. Mus musculus.
Family and domain databases
Gene3D	1.10.238.10. 2 hits.
InterPro	IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. [Graphical view]
Pfam	PF00036. efhand. 1 hit. [Graphical view]
SMART	SM00054. EFh. 3 hits. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 1 hit. PS50222. EF_HAND_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	MYL2. mouse.
NextBio	292733.
SOURCE	Search...

Entry information

Entry name

MLRV_MOUSE

Accession

Primary (citable) accession number: P51667
Secondary accession number(s): Q6P8P4, Q9QVP3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	April 3, 2007
Last modified:	May 1, 2013
This is version 108 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program