Reviewed, UniProtKB/Swiss-Prot P51665 (PSD7_HUMAN)

Names and origin

Protein names

Recommended name:
    26S proteasome non-ATPase regulatory subunit 7
Alternative name(s):
    26S proteasome regulatory subunit rpn8
    26S proteasome regulatory subunit S12
    Proteasome subunit p40
    Mov34 protein homolog

Gene names

Name:	PSMD7
Synonyms:	MOV34L

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
Sequence similarities	Belongs to the peptidase M67A family. Contains 1 MPN (JAB/Mov34) domain.
Sequence caution	The sequence AAH00338.1 differs from that shown. Reason: Miscellaneous discrepancy. Contaminating sequence. Potential poly-A sequence.

Ontologies

Keywords
Cellular component	Proteasome
PTM	Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	3D-structure
Gene Ontology (GO)
Biological process	anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Inferred from Experiment. Source: Reactome negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle Inferred from Experiment. Source: Reactome positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle Inferred from Experiment. Source: Reactome
Cellular component	cytosol Inferred from electronic annotation. Source: UniProtKB-KW proteasome regulatory particle Ref.1 Traceable author statement. Source: ProtInc
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PAAF1	Q9BRP4	1	EBI-357659,EBI-1056358
PSMC4	P43686	1	EBI-357659,EBI-743997
PSMD10	O75832	1	EBI-357659,EBI-752185
PSMD6	Q15008	1	EBI-357659,EBI-359701

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.3

Chain

2 – 324

323

26S proteasome non-ATPase regulatory subunit 7

PRO_0000213943

Regions

Domain

4 – 118

115

MPN

Compositional bias

286 – 324

39

Glu/Lys-rich

Amino acid modifications

Modified residue

186

1

Phosphothreonine Ref.3

Cross-link

180

Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.4

Experimental info

Sequence conflict

27

1

G → V in BAA08780. Ref.1

Sequence conflict

144 – 145

2

VH → DQ in BAA08780. Ref.1

Sequence conflict

216

1

A → G in BAA08780. Ref.1

Secondary structure

1

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324

Helix Strand Turn

Details...

Beta strand

7 – 11

5

Helix

13 – 28

16

Beta strand

36 – 56

21

Beta strand

58 – 60

3

Beta strand

67 – 70

4

Helix

72 – 83

12

Beta strand

86 – 88

3

Beta strand

90 – 96

7

Helix

105 – 112

8

Turn

113 – 115

3

Beta strand

120 – 124

5

Beta strand

134 – 143

10

Beta strand

152 – 164

13

Helix

166 – 178

13

Sequences

	Sequence		Length	Mass (Da)
	P51665-1 [UniParc]. Last modified May 10, 2002. Version 2. Checksum: 3F7B343996B102B7	FASTA	324	37,025
	10 20 30 40 50 60 MPELAVQKVV VHPLVLLSVV DHFNRIGKVG NQKRVVGVLL GSWQKKVLDV SNSFAVPFDE 70 80 90 100 110 120 DDKDDSVWFL DHDYLENMYG MFKKVNARER IVGWYHTGPK LHKNDIAINE LMKRYCPNSV 130 140 150 160 170 180 LVIIDVKPKD LGLPTEAYIS VEEVHDDGTP TSKTFEHVTS EIGAEEAEEV GVEHLLRDIK 190 200 210 220 230 240 DTTVGTLSQR ITNQVHGLKG LNSKLLDIRS YLEKVATGKL PINHQIIYQL QDVFNLLPDV 250 260 270 280 290 300 SLQEFVKAFY LKTNDQMVVV YLASLIRSVV ALHNLINNKI ANRDAEKKEG QEKEESKKDR 310 320 KEDKEKDKDK EKSDVKKEEK KEKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning of p40, a regulatory subunit of the human 26S proteasome, and a homolog of the Mov-34 gene product." Tsurumi C., DeMartino G.N., Slaughter C., Shimbara N., Tanaka K. Biochem. Biophys. Res. Commun. 210:600-608(1995) [PubMed: 7755639] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung and Skin.
[3]	"Mass spectrometric characterization of the affinity-purified human 26S proteasome complex." Wang X., Chen C.-F., Baker P.R., Chen P.-L., Kaiser P., Huang L. Biochemistry 46:3553-3565(2007) [PubMed: 17323924] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-186, CLEAVAGE OF INITIATOR METHIONINE, MASS SPECTROMETRY.
[4]	"Quantitative analysis of global ubiquitination in HeLa cells by mass spectrometry." Meierhofer D., Wang X., Huang L., Kaiser P. J. Proteome Res. 7:4566-4576(2008) [PubMed: 18781797] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-180, MASS SPECTROMETRY.
[5]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

D50063 mRNA. Translation: BAA08780.1.
BC000338 mRNA. Translation: AAH00338.1. Sequence problems.
BC012606 mRNA. Translation: AAH12606.1.

IPI

IPI00019927.

PIR

JC4154.
S65491.

RefSeq

NP_002802.2.

UniGene

Hs.440604

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2O95	X-ray	1.95	A/B	1-186	[»]
2O96	X-ray	3.00	A/B	1-177	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:27572N.

IntAct

P51665. 10 interactions.

Protein family/group databases

MEROPS

M67.973.

PTM databases

PhosphoSite

P51665.

Proteomic databases

PeptideAtlas

P51665.

PRIDE

P51665.

Genome annotation databases

Ensembl

ENSG00000103035. Homo sapiens. [Contig view]

GeneID

5713.

KEGG

hsa:5713.

Organism-specific databases

GeneCards

GC16P072888.

H-InvDB

HIX0013230.

HGNC

HGNC:9565. PSMD7.

MIM

157970. gene.

PharmGKB

PA33911.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

P51665.

HOVERGEN

P51665.

OMA

P51665. MLVVYLA.

Enzyme and pathway databases

Reactome

REACT_11045. Signaling by Wnt.
REACT_13. Metabolism of amino acids.
REACT_13635. Regulation of activated PAK-2p34 by proteasome mediated degradation.
REACT_152. Cell Cycle, Mitotic.
REACT_1538. Cell Cycle Checkpoints.
REACT_383. DNA Replication.
REACT_578. Apoptosis.
REACT_6185. HIV Infection.
REACT_6850. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
REACT_9035. APC/C:Cdh1-mediated degradation of Skp2.

Gene expression databases

ArrayExpress

P51665.

Bgee

P51665.

CleanEx

HS_PSMD7.

GermOnline

ENSG00000103035. Homo sapiens.

Family and domain databases

InterPro

IPR000555. Mov34_MPN_PAD1.
[Graphical view]

Pfam

PF01398. Mov34. 1 hit.
[Graphical view]

ProDom

PD363422. Mov34-1. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00232. JAB_MPN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

22194.

SOURCE

Search...

Entry information

Entry name

PSD7_HUMAN

Accession

Primary (citable) accession number: P51665
Secondary accession number(s): Q6PKI2, Q96E97

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	May 10, 2002
Last modified:	June 16, 2009
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)