ID   C11B1_SHEEP             Reviewed;         503 AA.
AC   P51663; O19181;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1998, sequence version 2.
DT   26-MAY-2009, entry version 57.
DE   RecName: Full=Cytochrome P450 11B1, mitochondrial;
DE   AltName: Full=CYPXIB1;
DE   AltName: Full=P450C11;
DE   AltName: Full=Steroid 11-beta-hydroxylase;
DE            EC=1.14.15.4;
DE   Flags: Precursor;
GN   Name=CYP11B1;
OS   Ovis aries (Sheep).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia;
OC   Pecora; Bovidae; Caprinae; Ovis.
OX   NCBI_TaxID=9940;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Merino; TISSUE=Adrenal cortex;
RX   MEDLINE=95092784; PubMed=7999785; DOI=10.1016/0167-4781(94)00190-E;
RA   Boon W.C., Roche P.J., Hammond V.E., Jeyaseelan K., Crawford R.J.,
RA   Coghlan J.P.;
RT   "Cloning and expression analysis of a cytochrome P-450(11 beta) cDNA
RT   in sheep.";
RL   Biochim. Biophys. Acta 1260:109-112(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC   TISSUE=Adrenal gland, and Liver;
RX   PubMed=10728821;
RA   Anwar A., Coghlan J.P., Jeyaseelan K.;
RT   "Structure of an ovine CYP11B1 gene.";
RL   DNA Seq. 8:357-374(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-77.
RX   MEDLINE=95102523; PubMed=7804143;
RA   Anwar A., Jeyaseelan K., Coghlan J.P.;
RT   "Molecular cloning and characterization of the ovine CYP11B1
RT   promoter.";
RL   Biochem. Mol. Biol. Int. 33:1169-1178(1994).
CC   -!- FUNCTION: Has steroid 11-beta-hydroxylase activity. In addition to
CC       this activity, the 18 or 19-hydroxylation of steroids and the
CC       aromatization of androstendione to estrone have also been ascribed
CC       to cytochrome P450 XIB.
CC   -!- CATALYTIC ACTIVITY: A steroid + reduced adrenal ferredoxin + O(2)
CC       = an 11-beta-hydroxysteroid + oxidized adrenal ferredoxin + H(2)O.
CC   -!- COFACTOR: Heme group (By similarity).
CC   -!- SUBCELLULAR LOCATION: Mitochondrion membrane.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family.
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DR   EMBL; L34337; AAA62308.1; -; mRNA.
DR   EMBL; L47569; AAA81576.1; -; Genomic_DNA.
DR   EMBL; U78478; AAB64249.1; -; Genomic_DNA.
DR   EMBL; U78477; AAB64248.1; -; mRNA.
DR   EMBL; L28716; AAA83384.1; -; Genomic_DNA.
DR   PIR; S52085; S52085.
DR   RefSeq; NP_001068568.1; -.
DR   UniGene; Oar.397; -.
DR   HSSP; P00189; 1SCC.
DR   GeneID; 767576; -.
DR   HOVERGEN; P51663; -.
DR   BRENDA; 1.14.15.4; 271.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0004507; F:steroid 11-beta-monooxygenase activity; IEA:EC.
DR   GO; GO:0006700; P:C21-steroid hormone biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017973; Cyt_P450_C.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   Gene3D; G3DSA:1.10.630.10; Cyt_P450; 1.
DR   PANTHER; PTHR19383; Cyt_P450; 1.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Heme; Iron; Membrane; Metal-binding; Mitochondrion; Monooxygenase;
KW   Oxidoreductase; Steroidogenesis; Transit peptide.
FT   TRANSIT       1     24       Mitochondrion.
FT   CHAIN        25    503       Cytochrome P450 11B1, mitochondrial.
FT                                /FTId=PRO_0000003606.
FT   METAL       450    450       Iron (heme axial ligand) (By similarity).
FT   CONFLICT     99     99       R -> S (in Ref. 2; AAB64248/AAB64249).
FT   CONFLICT    189    189       A -> G (in Ref. 1; AAA62308).
FT   CONFLICT    439    439       P -> R (in Ref. 1; AAA62308).
FT   CONFLICT    500    500       R -> G (in Ref. 2; AAB64248/AAB64249).
SQ   SEQUENCE   503 AA;  57784 MW;  968F2DF6EE7D595B CRC64;
     MALWAKARVW MAGPWLSLHR ARPLGTRASA APKAVLPFEA MPRCPGNKWM RVLQIWKEQG
     SENMHLDMHQ TFQELGPIFR YDVGGRHMVF VMLPEDVERL QQAESLHPQR MLLEPWLAYR
     QARGHKCGVF LLNGPQWRLD RLRLNPDVLS LPALQKYTPL VDGVARDFSQ TLKARVLQNA
     RGSLTLDIAP SVFRYTIEAS TLVLYGERLG LLTQQPNPDS LNFIHALEAM FKSTVQLMFV
     PRRLSRWTSS SMWREHFEAW DYIFQYANRA IQRIYQELAL GHPWHYSGIV AELLMRADMT
     LDTIKANTID LTAGSVDTTA FPLLMTLFEL ARNPEVQQAL RQESLVAEAR ISENPQRATT
     ELPLLRAALK ETLRLYPVGI TLERQVSSDL VLQNYHIPAG TLVKVLLYSL GRNPAVFARP
     ESYHPQRWLD RQGSGSRFPH LAFGFGMRQC LGRRVAEVEM LLLLHHVLKN FLVETLAQED
     IKMVYRFILM PSTLPLFTFR AIQ
//