Reviewed, UniProtKB/Swiss-Prot P51663 (C11B1_SHEEP)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 11B1, mitochondrial
Alternative name(s):
    CYPXIB1
    P450C11
    Steroid 11-beta-hydroxylase
    EC=1.14.15.4

Gene names

Name:

CYP11B1

Organism

Ovis aries (Sheep)

Taxonomic identifier

9940 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Has steroid 11-beta-hydroxylase activity. In addition to this activity, the 18 or 19-hydroxylation of steroids and the aromatization of androstendione to estrone have also been ascribed to cytochrome P450 XIB.
Catalytic activity	A steroid + reduced adrenal ferredoxin + O2 = an 11-beta-hydroxysteroid + oxidized adrenal ferredoxin + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Mitochondrion membrane.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Steroidogenesis
Cellular component	Membrane Mitochondrion
Domain	Transit peptide
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro steroid 11-beta-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 24	24	Mitochondrion
	Chain	25 – 503	479	Cytochrome P450 11B1, mitochondrial		PRO_0000003606
Sites
	Metal binding	450	1	Iron (heme axial ligand) By similarity
Experimental info
	Sequence conflict	99	1	R → S in AAB64248. Ref.2
	Sequence conflict	99	1	R → S in AAB64249. Ref.2
	Sequence conflict	189	1	A → G in AAA62308. Ref.1
	Sequence conflict	439	1	P → R in AAA62308. Ref.1
	Sequence conflict	500	1	R → G in AAB64248. Ref.2
	Sequence conflict	500	1	R → G in AAB64249. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P51663-1 [UniParc]. Last modified July 15, 1998. Version 2. Checksum: 968F2DF6EE7D595B	FASTA	503	57,784
	10 20 30 40 50 60 MALWAKARVW MAGPWLSLHR ARPLGTRASA APKAVLPFEA MPRCPGNKWM RVLQIWKEQG 70 80 90 100 110 120 SENMHLDMHQ TFQELGPIFR YDVGGRHMVF VMLPEDVERL QQAESLHPQR MLLEPWLAYR 130 140 150 160 170 180 QARGHKCGVF LLNGPQWRLD RLRLNPDVLS LPALQKYTPL VDGVARDFSQ TLKARVLQNA 190 200 210 220 230 240 RGSLTLDIAP SVFRYTIEAS TLVLYGERLG LLTQQPNPDS LNFIHALEAM FKSTVQLMFV 250 260 270 280 290 300 PRRLSRWTSS SMWREHFEAW DYIFQYANRA IQRIYQELAL GHPWHYSGIV AELLMRADMT 310 320 330 340 350 360 LDTIKANTID LTAGSVDTTA FPLLMTLFEL ARNPEVQQAL RQESLVAEAR ISENPQRATT 370 380 390 400 410 420 ELPLLRAALK ETLRLYPVGI TLERQVSSDL VLQNYHIPAG TLVKVLLYSL GRNPAVFARP 430 440 450 460 470 480 ESYHPQRWLD RQGSGSRFPH LAFGFGMRQC LGRRVAEVEM LLLLHHVLKN FLVETLAQED 490 500 IKMVYRFILM PSTLPLFTFR AIQ

References

[1]	"Cloning and expression analysis of a cytochrome P-450(11 beta) cDNA in sheep." Boon W.C., Roche P.J., Hammond V.E., Jeyaseelan K., Crawford R.J., Coghlan J.P. Biochim. Biophys. Acta 1260:109-112(1995) [PubMed: 7999785] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Merino. Tissue: Adrenal cortex.
[2]	"Structure of an ovine CYP11B1 gene." Anwar A., Coghlan J.P., Jeyaseelan K. DNA Seq. 8:357-374(1998) [PubMed: 10728821] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Tissue: Adrenal gland and Liver.
[3]	"Molecular cloning and characterization of the ovine CYP11B1 promoter." Anwar A., Jeyaseelan K., Coghlan J.P. Biochem. Mol. Biol. Int. 33:1169-1178(1994) [PubMed: 7804143] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-77.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L34337 mRNA. Translation: AAA62308.1. L47569 Genomic DNA. Translation: AAA81576.1. U78478 Genomic DNA. Translation: AAB64249.1. U78477 mRNA. Translation: AAB64248.1. L28716 Genomic DNA. Translation: AAA83384.1.
PIR	S52085.
RefSeq	NP_001068568.1.
UniGene	Oar.397
3D structure databases
HSSP	HSSP built from PDB template 1SCC based on UniProtKB P00189.
ModBase	Search...
Genome annotation databases
GeneID	767576.
Organism-specific databases
CTD	767576.
Phylogenomic databases
HOVERGEN	P51663.
Enzyme and pathway databases
BRENDA	1.14.15.4. 271.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C11B1_SHEEP

Accession

Primary (citable) accession number: P51663
Secondary accession number(s): O19181

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	July 15, 1998
Last modified:	October 13, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)