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P51663 (C11B1_SHEEP) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 11B1, mitochondrial
Alternative name(s):
CYPXIB1
Cytochrome P450C11
Steroid 11-beta-hydroxylase
EC=1.14.15.4
Gene names
Name:CYP11B1
OrganismOvis aries (Sheep) [Complete proteome]
Taxonomic identifier9940 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis

Protein attributes

Sequence length503 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Has steroid 11-beta-hydroxylase activity. In addition to this activity, the 18 or 19-hydroxylation of steroids and the aromatization of androstendione to estrone have also been ascribed to cytochrome P450 XIB.

Catalytic activity

A steroid + reduced adrenodoxin + O2 = an 11-beta-hydroxysteroid + oxidized adrenodoxin + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Mitochondrion membrane.

Sequence similarities

Belongs to the cytochrome P450 family.

Ontologies

Keywords
   Biological processSteroidogenesis
   Cellular componentMembrane
Mitochondrion
   DomainTransit peptide
   LigandHeme
Iron
Metal-binding
   Molecular functionMonooxygenase
Oxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processsteroid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentmitochondrial membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionheme binding

Inferred from electronic annotation. Source: InterPro

iron ion binding

Inferred from electronic annotation. Source: InterPro

steroid 11-beta-monooxygenase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2424Mitochondrion
Chain25 – 503479Cytochrome P450 11B1, mitochondrial
PRO_0000003606

Sites

Metal binding4501Iron (heme axial ligand) By similarity

Experimental info

Sequence conflict991R → S in AAB64248. Ref.2
Sequence conflict991R → S in AAB64249. Ref.2
Sequence conflict1891A → G in AAA62308. Ref.1
Sequence conflict4391P → R in AAA62308. Ref.1
Sequence conflict5001R → G in AAB64248. Ref.2
Sequence conflict5001R → G in AAB64249. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P51663 [UniParc].

Last modified July 15, 1998. Version 2.
Checksum: 968F2DF6EE7D595B

FASTA50357,784
        10         20         30         40         50         60 
MALWAKARVW MAGPWLSLHR ARPLGTRASA APKAVLPFEA MPRCPGNKWM RVLQIWKEQG 

        70         80         90        100        110        120 
SENMHLDMHQ TFQELGPIFR YDVGGRHMVF VMLPEDVERL QQAESLHPQR MLLEPWLAYR 

       130        140        150        160        170        180 
QARGHKCGVF LLNGPQWRLD RLRLNPDVLS LPALQKYTPL VDGVARDFSQ TLKARVLQNA 

       190        200        210        220        230        240 
RGSLTLDIAP SVFRYTIEAS TLVLYGERLG LLTQQPNPDS LNFIHALEAM FKSTVQLMFV 

       250        260        270        280        290        300 
PRRLSRWTSS SMWREHFEAW DYIFQYANRA IQRIYQELAL GHPWHYSGIV AELLMRADMT 

       310        320        330        340        350        360 
LDTIKANTID LTAGSVDTTA FPLLMTLFEL ARNPEVQQAL RQESLVAEAR ISENPQRATT 

       370        380        390        400        410        420 
ELPLLRAALK ETLRLYPVGI TLERQVSSDL VLQNYHIPAG TLVKVLLYSL GRNPAVFARP 

       430        440        450        460        470        480 
ESYHPQRWLD RQGSGSRFPH LAFGFGMRQC LGRRVAEVEM LLLLHHVLKN FLVETLAQED 

       490        500 
IKMVYRFILM PSTLPLFTFR AIQ 

« Hide

References

[1]"Cloning and expression analysis of a cytochrome P-450(11 beta) cDNA in sheep."
Boon W.C., Roche P.J., Hammond V.E., Jeyaseelan K., Crawford R.J., Coghlan J.P.
Biochim. Biophys. Acta 1260:109-112(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Merino.
Tissue: Adrenal cortex.
[2]"Structure of an ovine CYP11B1 gene."
Anwar A., Coghlan J.P., Jeyaseelan K.
DNA Seq. 8:357-374(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Tissue: Adrenal gland and Liver.
[3]"Molecular cloning and characterization of the ovine CYP11B1 promoter."
Anwar A., Jeyaseelan K., Coghlan J.P.
Biochem. Mol. Biol. Int. 33:1169-1178(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-77.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L34337 mRNA. Translation: AAA62308.1.
L47569 Genomic DNA. Translation: AAA81576.1.
U78478 Genomic DNA. Translation: AAB64249.1.
U78477 mRNA. Translation: AAB64248.1.
L28716 Genomic DNA. Translation: AAA83384.1.
PIRS52085.
RefSeqNP_001068568.1. NM_001075100.2.
UniGeneOar.397.

3D structure databases

ProteinModelPortalP51663.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID767576.

Organism-specific databases

CTD1584.

Phylogenomic databases

HOVERGENHBG051098.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameC11B1_SHEEP
AccessionPrimary (citable) accession number: P51663
Secondary accession number(s): O19181
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 15, 1998
Last modified: February 19, 2014
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families