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P51661 (DHI2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Corticosteroid 11-beta-dehydrogenase isozyme 2

EC=1.1.1.-
Alternative name(s):
11-beta-hydroxysteroid dehydrogenase type 2
Short name=11-DH2
Short name=11-beta-HSD2
NAD-dependent 11-beta-hydroxysteroid dehydrogenase
Gene names
Name:Hsd11b2
Synonyms:Hsd11k
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length386 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids.

Catalytic activity

An 11-beta-hydroxysteroid + NAD+ = an 11-oxosteroid + NADH.

Enzyme regulation

Inhibited by glycyrrhetinic acid, carbenoloxone and 11-alpha-OH-progesterone By similarity.

Subunit structure

Interacts with ligand-free cytoplasmic NR3C2 By similarity.

Subcellular location

Microsome. Endoplasmic reticulum Potential.

Tissue specificity

Highly expressed in kidney. Also found in colon and small intestine. Not expressed in the adrenal gland.

Sequence similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Sequence caution

The sequence CAA62219.1 differs from that shown. Reason: Frameshift at position 383.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 386386Corticosteroid 11-beta-dehydrogenase isozyme 2
PRO_0000054628

Regions

Nucleotide binding82 – 11130NAD By similarity

Sites

Active site2321Proton acceptor By similarity
Binding site2191Substrate By similarity

Experimental info

Sequence conflict491Q → L in AAC60711. Ref.1
Sequence conflict491Q → L in CAA62218. Ref.1
Sequence conflict521L → M in AAC60711. Ref.1
Sequence conflict521L → M in CAA62218. Ref.1
Sequence conflict1781E → G in AAC60711. Ref.1
Sequence conflict1781E → G in CAA62218. Ref.1
Sequence conflict2161T → P in AAC60711. Ref.1
Sequence conflict2161T → P in CAA62218. Ref.1
Sequence conflict2651F → S in AAC60711. Ref.1
Sequence conflict2651F → S in CAA62218. Ref.1
Sequence conflict2681D → A in AAC60711. Ref.1
Sequence conflict2681D → A in CAA62218. Ref.1
Sequence conflict2971E → A in AAC60711. Ref.1
Sequence conflict2971E → A in CAA62218. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P51661 [UniParc].

Last modified April 30, 2003. Version 2.
Checksum: 687FD253568487AB

FASTA38642,187
        10         20         30         40         50         60 
MERWPWPSGG AWLLVAARAL LQLLRSDLRL GRPLLAALAL LAALDWLCQR LLPPPAALVV 

        70         80         90        100        110        120 
LAGAGWIALS RLARPPRLPV ATRAVLITGC DTGFGKETAK KLDAMGFTVL ATVLDLNSPG 

       130        140        150        160        170        180 
ALELRDLCSP RLKLLQMDLT KAEDISRVLE ITKAHTASTG LWGLVNNAGL NIVVADVELS 

       190        200        210        220        230        240 
PVATFRKCME VNFFGALELT KGLLPLLRHS RGRIVTVGSP AGDMPYPCLA AYGTSKAAIA 

       250        260        270        280        290        300 
LLMDTFGCEL LPWGIKVSII KPGCFKTDAV TNVNLWEKRK QLLLANIPRE LLQAYGEDYI 

       310        320        330        340        350        360 
EHVHGQFLNS LRMALPDLSP VVDAIIDALL AAQPRSRYYP GRGLGLMYFI HHYLPEGLRR 

       370        380 
CFLQNFFINH LLPRALRPGQ HGPAPA 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of the mouse 11 beta-hydroxysteroid dehydrogenase type 2 gene: tissue specific expression and localization in distal convoluted tubules and collecting ducts of the kidney."
Cole T.J.
Endocrinology 136:4693-4696(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/Sv.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S79554 expand/collapse EMBL AC list , S79550, S79551, S79552, S79553 Genomic DNA. Translation: AAC60711.1. Frameshift.
X90646 Genomic DNA. Translation: CAA62218.1.
X90647 Genomic DNA. Translation: CAA62219.1. Frameshift.
BC014753 mRNA. Translation: AAH14753.1.
CCDSCCDS40460.1.
PIRS60188.
RefSeqNP_032315.2. NM_008289.2.
UniGeneMm.5079.

3D structure databases

ProteinModelPortalP51661.
SMRP51661. Positions 81-339.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP51661. 3 interactions.
MINTMINT-4093038.

Chemistry

BindingDBP51661.
ChEMBLCHEMBL3490.

PTM databases

PhosphoSiteP51661.

Proteomic databases

MaxQBP51661.
PaxDbP51661.
PRIDEP51661.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000034363; ENSMUSP00000034363; ENSMUSG00000031891.
GeneID15484.
KEGGmmu:15484.
UCSCuc009nde.1. mouse.

Organism-specific databases

CTD3291.
MGIMGI:104720. Hsd11b2.

Phylogenomic databases

eggNOGCOG1028.
GeneTreeENSGT00650000093222.
HOVERGENHBG005482.
InParanoidP51661.
KOK00071.
OMACFKTESV.
OrthoDBEOG7FXZZX.
PhylomeDBP51661.
TreeFamTF325617.

Enzyme and pathway databases

BRENDA1.1.1.146. 3474.

Gene expression databases

ArrayExpressP51661.
BgeeP51661.
CleanExMM_HSD11B2.
GenevestigatorP51661.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSPR00081. GDHRDH.
PROSITEPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio288342.
PROP51661.
SOURCESearch...

Entry information

Entry nameDHI2_MOUSE
AccessionPrimary (citable) accession number: P51661
Secondary accession number(s): Q91WK3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: April 30, 2003
Last modified: July 9, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot