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P51661

- DHI2_MOUSE

UniProt

P51661 - DHI2_MOUSE

Protein

Corticosteroid 11-beta-dehydrogenase isozyme 2

Gene

Hsd11b2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 2 (30 Apr 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids.

    Catalytic activityi

    An 11-beta-hydroxysteroid + NAD+ = an 11-oxosteroid + NADH.

    Enzyme regulationi

    Inhibited by glycyrrhetinic acid, carbenoloxone and 11-alpha-OH-progesterone.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei219 – 2191SubstrateBy similarity
    Active sitei232 – 2321Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi82 – 11130NADBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. 11-beta-hydroxysteroid dehydrogenase [NAD(P)] activity Source: Ensembl
    2. NAD binding Source: Ensembl
    3. steroid binding Source: Ensembl

    GO - Biological processi

    1. female pregnancy Source: Ensembl
    2. glucocorticoid metabolic process Source: Ensembl
    3. regulation of blood volume by renal aldosterone Source: Ensembl
    4. response to drug Source: Ensembl
    5. response to food Source: Ensembl
    6. response to glucocorticoid Source: Ensembl
    7. response to hypoxia Source: Ensembl
    8. response to insulin Source: Ensembl

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BRENDAi1.1.1.146. 3474.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Corticosteroid 11-beta-dehydrogenase isozyme 2 (EC:1.1.1.-)
    Alternative name(s):
    11-beta-hydroxysteroid dehydrogenase type 2
    Short name:
    11-DH2
    Short name:
    11-beta-HSD2
    NAD-dependent 11-beta-hydroxysteroid dehydrogenase
    Gene namesi
    Name:Hsd11b2
    Synonyms:Hsd11k
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:104720. Hsd11b2.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 386386Corticosteroid 11-beta-dehydrogenase isozyme 2PRO_0000054628Add
    BLAST

    Proteomic databases

    MaxQBiP51661.
    PaxDbiP51661.
    PRIDEiP51661.

    PTM databases

    PhosphoSiteiP51661.

    Expressioni

    Tissue specificityi

    Highly expressed in kidney. Also found in colon and small intestine. Not expressed in the adrenal gland.

    Gene expression databases

    ArrayExpressiP51661.
    BgeeiP51661.
    CleanExiMM_HSD11B2.
    GenevestigatoriP51661.

    Interactioni

    Subunit structurei

    Interacts with ligand-free cytoplasmic NR3C2.By similarity

    Protein-protein interaction databases

    IntActiP51661. 3 interactions.
    MINTiMINT-4093038.

    Structurei

    3D structure databases

    ProteinModelPortaliP51661.
    SMRiP51661. Positions 81-339.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1028.
    GeneTreeiENSGT00650000093222.
    HOVERGENiHBG005482.
    InParanoidiP51661.
    KOiK00071.
    OMAiCFKTESV.
    OrthoDBiEOG7FXZZX.
    PhylomeDBiP51661.
    TreeFamiTF325617.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view]
    PfamiPF00106. adh_short. 1 hit.
    [Graphical view]
    PRINTSiPR00081. GDHRDH.
    PROSITEiPS00061. ADH_SHORT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P51661-1 [UniParc]FASTAAdd to Basket

    « Hide

    MERWPWPSGG AWLLVAARAL LQLLRSDLRL GRPLLAALAL LAALDWLCQR    50
    LLPPPAALVV LAGAGWIALS RLARPPRLPV ATRAVLITGC DTGFGKETAK 100
    KLDAMGFTVL ATVLDLNSPG ALELRDLCSP RLKLLQMDLT KAEDISRVLE 150
    ITKAHTASTG LWGLVNNAGL NIVVADVELS PVATFRKCME VNFFGALELT 200
    KGLLPLLRHS RGRIVTVGSP AGDMPYPCLA AYGTSKAAIA LLMDTFGCEL 250
    LPWGIKVSII KPGCFKTDAV TNVNLWEKRK QLLLANIPRE LLQAYGEDYI 300
    EHVHGQFLNS LRMALPDLSP VVDAIIDALL AAQPRSRYYP GRGLGLMYFI 350
    HHYLPEGLRR CFLQNFFINH LLPRALRPGQ HGPAPA 386
    Length:386
    Mass (Da):42,187
    Last modified:April 30, 2003 - v2
    Checksum:i687FD253568487AB
    GO

    Sequence cautioni

    The sequence CAA62219.1 differs from that shown. Reason: Frameshift at position 383.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti49 – 491Q → L in AAC60711. (PubMed:7664690)Curated
    Sequence conflicti49 – 491Q → L in CAA62218. (PubMed:7664690)Curated
    Sequence conflicti52 – 521L → M in AAC60711. (PubMed:7664690)Curated
    Sequence conflicti52 – 521L → M in CAA62218. (PubMed:7664690)Curated
    Sequence conflicti178 – 1781E → G in AAC60711. (PubMed:7664690)Curated
    Sequence conflicti178 – 1781E → G in CAA62218. (PubMed:7664690)Curated
    Sequence conflicti216 – 2161T → P in AAC60711. (PubMed:7664690)Curated
    Sequence conflicti216 – 2161T → P in CAA62218. (PubMed:7664690)Curated
    Sequence conflicti265 – 2651F → S in AAC60711. (PubMed:7664690)Curated
    Sequence conflicti265 – 2651F → S in CAA62218. (PubMed:7664690)Curated
    Sequence conflicti268 – 2681D → A in AAC60711. (PubMed:7664690)Curated
    Sequence conflicti268 – 2681D → A in CAA62218. (PubMed:7664690)Curated
    Sequence conflicti297 – 2971E → A in AAC60711. (PubMed:7664690)Curated
    Sequence conflicti297 – 2971E → A in CAA62218. (PubMed:7664690)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S79554
    , S79550, S79551, S79552, S79553 Genomic DNA. Translation: AAC60711.1. Frameshift.
    X90646 Genomic DNA. Translation: CAA62218.1.
    X90647 Genomic DNA. Translation: CAA62219.1. Frameshift.
    BC014753 mRNA. Translation: AAH14753.1.
    CCDSiCCDS40460.1.
    PIRiS60188.
    RefSeqiNP_032315.2. NM_008289.2.
    UniGeneiMm.5079.

    Genome annotation databases

    EnsembliENSMUST00000034363; ENSMUSP00000034363; ENSMUSG00000031891.
    GeneIDi15484.
    KEGGimmu:15484.
    UCSCiuc009nde.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S79554
    , S79550 , S79551 , S79552 , S79553 Genomic DNA. Translation: AAC60711.1 . Frameshift.
    X90646 Genomic DNA. Translation: CAA62218.1 .
    X90647 Genomic DNA. Translation: CAA62219.1 . Frameshift.
    BC014753 mRNA. Translation: AAH14753.1 .
    CCDSi CCDS40460.1.
    PIRi S60188.
    RefSeqi NP_032315.2. NM_008289.2.
    UniGenei Mm.5079.

    3D structure databases

    ProteinModelPortali P51661.
    SMRi P51661. Positions 81-339.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P51661. 3 interactions.
    MINTi MINT-4093038.

    Chemistry

    BindingDBi P51661.
    ChEMBLi CHEMBL3490.

    PTM databases

    PhosphoSitei P51661.

    Proteomic databases

    MaxQBi P51661.
    PaxDbi P51661.
    PRIDEi P51661.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000034363 ; ENSMUSP00000034363 ; ENSMUSG00000031891 .
    GeneIDi 15484.
    KEGGi mmu:15484.
    UCSCi uc009nde.1. mouse.

    Organism-specific databases

    CTDi 3291.
    MGIi MGI:104720. Hsd11b2.

    Phylogenomic databases

    eggNOGi COG1028.
    GeneTreei ENSGT00650000093222.
    HOVERGENi HBG005482.
    InParanoidi P51661.
    KOi K00071.
    OMAi CFKTESV.
    OrthoDBi EOG7FXZZX.
    PhylomeDBi P51661.
    TreeFami TF325617.

    Enzyme and pathway databases

    BRENDAi 1.1.1.146. 3474.

    Miscellaneous databases

    NextBioi 288342.
    PROi P51661.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P51661.
    Bgeei P51661.
    CleanExi MM_HSD11B2.
    Genevestigatori P51661.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view ]
    Pfami PF00106. adh_short. 1 hit.
    [Graphical view ]
    PRINTSi PR00081. GDHRDH.
    PROSITEi PS00061. ADH_SHORT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of the mouse 11 beta-hydroxysteroid dehydrogenase type 2 gene: tissue specific expression and localization in distal convoluted tubules and collecting ducts of the kidney."
      Cole T.J.
      Endocrinology 136:4693-4696(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/Sv.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.

    Entry informationi

    Entry nameiDHI2_MOUSE
    AccessioniPrimary (citable) accession number: P51661
    Secondary accession number(s): Q91WK3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: April 30, 2003
    Last modified: October 1, 2014
    This is version 116 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3