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Protein

Corticosteroid 11-beta-dehydrogenase isozyme 2

Gene

Hsd11b2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids.

Catalytic activityi

An 11-beta-hydroxysteroid + NAD+ = an 11-oxosteroid + NADH.

Enzyme regulationi

Inhibited by glycyrrhetinic acid, carbenoloxone and 11-alpha-OH-progesterone.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei219 – 2191SubstrateBy similarity
Active sitei232 – 2321Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi82 – 11130NADBy similarityAdd
BLAST

GO - Molecular functioni

  1. 11-beta-hydroxysteroid dehydrogenase [NAD(P)] activity Source: Ensembl
  2. NAD binding Source: Ensembl
  3. steroid binding Source: Ensembl

GO - Biological processi

  1. female pregnancy Source: MGI
  2. glucocorticoid metabolic process Source: Ensembl
  3. regulation of blood volume by renal aldosterone Source: Ensembl
  4. response to drug Source: Ensembl
  5. response to food Source: Ensembl
  6. response to glucocorticoid Source: Ensembl
  7. response to hypoxia Source: Ensembl
  8. response to insulin Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BRENDAi1.1.1.146. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Corticosteroid 11-beta-dehydrogenase isozyme 2 (EC:1.1.1.-)
Alternative name(s):
11-beta-hydroxysteroid dehydrogenase type 2
Short name:
11-DH2
Short name:
11-beta-HSD2
NAD-dependent 11-beta-hydroxysteroid dehydrogenase
Gene namesi
Name:Hsd11b2
Synonyms:Hsd11k
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:104720. Hsd11b2.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 386386Corticosteroid 11-beta-dehydrogenase isozyme 2PRO_0000054628Add
BLAST

Proteomic databases

MaxQBiP51661.
PaxDbiP51661.
PRIDEiP51661.

PTM databases

PhosphoSiteiP51661.

Expressioni

Tissue specificityi

Highly expressed in kidney. Also found in colon and small intestine. Not expressed in the adrenal gland.

Gene expression databases

BgeeiP51661.
CleanExiMM_HSD11B2.
ExpressionAtlasiP51661. baseline and differential.
GenevestigatoriP51661.

Interactioni

Subunit structurei

Interacts with ligand-free cytoplasmic NR3C2.By similarity

Protein-protein interaction databases

IntActiP51661. 3 interactions.
MINTiMINT-4093038.

Structurei

3D structure databases

ProteinModelPortaliP51661.
SMRiP51661. Positions 81-339.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1028.
GeneTreeiENSGT00770000120485.
HOVERGENiHBG005482.
InParanoidiP51661.
KOiK00071.
OMAiNAGHNEV.
OrthoDBiEOG7FXZZX.
PhylomeDBiP51661.
TreeFamiTF325617.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P51661-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MERWPWPSGG AWLLVAARAL LQLLRSDLRL GRPLLAALAL LAALDWLCQR
60 70 80 90 100
LLPPPAALVV LAGAGWIALS RLARPPRLPV ATRAVLITGC DTGFGKETAK
110 120 130 140 150
KLDAMGFTVL ATVLDLNSPG ALELRDLCSP RLKLLQMDLT KAEDISRVLE
160 170 180 190 200
ITKAHTASTG LWGLVNNAGL NIVVADVELS PVATFRKCME VNFFGALELT
210 220 230 240 250
KGLLPLLRHS RGRIVTVGSP AGDMPYPCLA AYGTSKAAIA LLMDTFGCEL
260 270 280 290 300
LPWGIKVSII KPGCFKTDAV TNVNLWEKRK QLLLANIPRE LLQAYGEDYI
310 320 330 340 350
EHVHGQFLNS LRMALPDLSP VVDAIIDALL AAQPRSRYYP GRGLGLMYFI
360 370 380
HHYLPEGLRR CFLQNFFINH LLPRALRPGQ HGPAPA
Length:386
Mass (Da):42,187
Last modified:April 30, 2003 - v2
Checksum:i687FD253568487AB
GO

Sequence cautioni

The sequence CAA62219.1 differs from that shown. Reason: Frameshift at position 383. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti49 – 491Q → L in AAC60711. (PubMed:7664690)Curated
Sequence conflicti49 – 491Q → L in CAA62218. (PubMed:7664690)Curated
Sequence conflicti52 – 521L → M in AAC60711. (PubMed:7664690)Curated
Sequence conflicti52 – 521L → M in CAA62218. (PubMed:7664690)Curated
Sequence conflicti178 – 1781E → G in AAC60711. (PubMed:7664690)Curated
Sequence conflicti178 – 1781E → G in CAA62218. (PubMed:7664690)Curated
Sequence conflicti216 – 2161T → P in AAC60711. (PubMed:7664690)Curated
Sequence conflicti216 – 2161T → P in CAA62218. (PubMed:7664690)Curated
Sequence conflicti265 – 2651F → S in AAC60711. (PubMed:7664690)Curated
Sequence conflicti265 – 2651F → S in CAA62218. (PubMed:7664690)Curated
Sequence conflicti268 – 2681D → A in AAC60711. (PubMed:7664690)Curated
Sequence conflicti268 – 2681D → A in CAA62218. (PubMed:7664690)Curated
Sequence conflicti297 – 2971E → A in AAC60711. (PubMed:7664690)Curated
Sequence conflicti297 – 2971E → A in CAA62218. (PubMed:7664690)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S79554
, S79550, S79551, S79552, S79553 Genomic DNA. Translation: AAC60711.1. Frameshift.
X90646 Genomic DNA. Translation: CAA62218.1.
X90647 Genomic DNA. Translation: CAA62219.1. Frameshift.
BC014753 mRNA. Translation: AAH14753.1.
CCDSiCCDS40460.1.
PIRiS60188.
RefSeqiNP_032315.2. NM_008289.2.
UniGeneiMm.5079.

Genome annotation databases

EnsembliENSMUST00000034363; ENSMUSP00000034363; ENSMUSG00000031891.
GeneIDi15484.
KEGGimmu:15484.
UCSCiuc009nde.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S79554
, S79550, S79551, S79552, S79553 Genomic DNA. Translation: AAC60711.1. Frameshift.
X90646 Genomic DNA. Translation: CAA62218.1.
X90647 Genomic DNA. Translation: CAA62219.1. Frameshift.
BC014753 mRNA. Translation: AAH14753.1.
CCDSiCCDS40460.1.
PIRiS60188.
RefSeqiNP_032315.2. NM_008289.2.
UniGeneiMm.5079.

3D structure databases

ProteinModelPortaliP51661.
SMRiP51661. Positions 81-339.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP51661. 3 interactions.
MINTiMINT-4093038.

Chemistry

BindingDBiP51661.
ChEMBLiCHEMBL3490.

PTM databases

PhosphoSiteiP51661.

Proteomic databases

MaxQBiP51661.
PaxDbiP51661.
PRIDEiP51661.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034363; ENSMUSP00000034363; ENSMUSG00000031891.
GeneIDi15484.
KEGGimmu:15484.
UCSCiuc009nde.1. mouse.

Organism-specific databases

CTDi3291.
MGIiMGI:104720. Hsd11b2.

Phylogenomic databases

eggNOGiCOG1028.
GeneTreeiENSGT00770000120485.
HOVERGENiHBG005482.
InParanoidiP51661.
KOiK00071.
OMAiNAGHNEV.
OrthoDBiEOG7FXZZX.
PhylomeDBiP51661.
TreeFamiTF325617.

Enzyme and pathway databases

BRENDAi1.1.1.146. 3474.

Miscellaneous databases

NextBioi288342.
PROiP51661.
SOURCEiSearch...

Gene expression databases

BgeeiP51661.
CleanExiMM_HSD11B2.
ExpressionAtlasiP51661. baseline and differential.
GenevestigatoriP51661.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of the mouse 11 beta-hydroxysteroid dehydrogenase type 2 gene: tissue specific expression and localization in distal convoluted tubules and collecting ducts of the kidney."
    Cole T.J.
    Endocrinology 136:4693-4696(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/Sv.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiDHI2_MOUSE
AccessioniPrimary (citable) accession number: P51661
Secondary accession number(s): Q91WK3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: April 30, 2003
Last modified: January 7, 2015
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.