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P51661

- DHI2_MOUSE

UniProt

P51661 - DHI2_MOUSE

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Protein

Corticosteroid 11-beta-dehydrogenase isozyme 2

Gene

Hsd11b2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids.

Catalytic activityi

An 11-beta-hydroxysteroid + NAD+ = an 11-oxosteroid + NADH.

Enzyme regulationi

Inhibited by glycyrrhetinic acid, carbenoloxone and 11-alpha-OH-progesterone.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei219 – 2191SubstrateBy similarity
Active sitei232 – 2321Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi82 – 11130NADBy similarityAdd
BLAST

GO - Molecular functioni

  1. 11-beta-hydroxysteroid dehydrogenase [NAD(P)] activity Source: Ensembl
  2. NAD binding Source: Ensembl
  3. steroid binding Source: Ensembl

GO - Biological processi

  1. female pregnancy Source: Ensembl
  2. glucocorticoid metabolic process Source: Ensembl
  3. regulation of blood volume by renal aldosterone Source: Ensembl
  4. response to drug Source: Ensembl
  5. response to food Source: Ensembl
  6. response to glucocorticoid Source: Ensembl
  7. response to hypoxia Source: Ensembl
  8. response to insulin Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BRENDAi1.1.1.146. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Corticosteroid 11-beta-dehydrogenase isozyme 2 (EC:1.1.1.-)
Alternative name(s):
11-beta-hydroxysteroid dehydrogenase type 2
Short name:
11-DH2
Short name:
11-beta-HSD2
NAD-dependent 11-beta-hydroxysteroid dehydrogenase
Gene namesi
Name:Hsd11b2
Synonyms:Hsd11k
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:104720. Hsd11b2.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 386386Corticosteroid 11-beta-dehydrogenase isozyme 2PRO_0000054628Add
BLAST

Proteomic databases

MaxQBiP51661.
PaxDbiP51661.
PRIDEiP51661.

PTM databases

PhosphoSiteiP51661.

Expressioni

Tissue specificityi

Highly expressed in kidney. Also found in colon and small intestine. Not expressed in the adrenal gland.

Gene expression databases

BgeeiP51661.
CleanExiMM_HSD11B2.
ExpressionAtlasiP51661. baseline and differential.
GenevestigatoriP51661.

Interactioni

Subunit structurei

Interacts with ligand-free cytoplasmic NR3C2.By similarity

Protein-protein interaction databases

IntActiP51661. 3 interactions.
MINTiMINT-4093038.

Structurei

3D structure databases

ProteinModelPortaliP51661.
SMRiP51661. Positions 81-339.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1028.
GeneTreeiENSGT00770000120485.
HOVERGENiHBG005482.
InParanoidiP51661.
KOiK00071.
OMAiCFKTESV.
OrthoDBiEOG7FXZZX.
PhylomeDBiP51661.
TreeFamiTF325617.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P51661-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MERWPWPSGG AWLLVAARAL LQLLRSDLRL GRPLLAALAL LAALDWLCQR
60 70 80 90 100
LLPPPAALVV LAGAGWIALS RLARPPRLPV ATRAVLITGC DTGFGKETAK
110 120 130 140 150
KLDAMGFTVL ATVLDLNSPG ALELRDLCSP RLKLLQMDLT KAEDISRVLE
160 170 180 190 200
ITKAHTASTG LWGLVNNAGL NIVVADVELS PVATFRKCME VNFFGALELT
210 220 230 240 250
KGLLPLLRHS RGRIVTVGSP AGDMPYPCLA AYGTSKAAIA LLMDTFGCEL
260 270 280 290 300
LPWGIKVSII KPGCFKTDAV TNVNLWEKRK QLLLANIPRE LLQAYGEDYI
310 320 330 340 350
EHVHGQFLNS LRMALPDLSP VVDAIIDALL AAQPRSRYYP GRGLGLMYFI
360 370 380
HHYLPEGLRR CFLQNFFINH LLPRALRPGQ HGPAPA
Length:386
Mass (Da):42,187
Last modified:April 30, 2003 - v2
Checksum:i687FD253568487AB
GO

Sequence cautioni

The sequence CAA62219.1 differs from that shown. Reason: Frameshift at position 383. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti49 – 491Q → L in AAC60711. (PubMed:7664690)Curated
Sequence conflicti49 – 491Q → L in CAA62218. (PubMed:7664690)Curated
Sequence conflicti52 – 521L → M in AAC60711. (PubMed:7664690)Curated
Sequence conflicti52 – 521L → M in CAA62218. (PubMed:7664690)Curated
Sequence conflicti178 – 1781E → G in AAC60711. (PubMed:7664690)Curated
Sequence conflicti178 – 1781E → G in CAA62218. (PubMed:7664690)Curated
Sequence conflicti216 – 2161T → P in AAC60711. (PubMed:7664690)Curated
Sequence conflicti216 – 2161T → P in CAA62218. (PubMed:7664690)Curated
Sequence conflicti265 – 2651F → S in AAC60711. (PubMed:7664690)Curated
Sequence conflicti265 – 2651F → S in CAA62218. (PubMed:7664690)Curated
Sequence conflicti268 – 2681D → A in AAC60711. (PubMed:7664690)Curated
Sequence conflicti268 – 2681D → A in CAA62218. (PubMed:7664690)Curated
Sequence conflicti297 – 2971E → A in AAC60711. (PubMed:7664690)Curated
Sequence conflicti297 – 2971E → A in CAA62218. (PubMed:7664690)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S79554
, S79550, S79551, S79552, S79553 Genomic DNA. Translation: AAC60711.1. Frameshift.
X90646 Genomic DNA. Translation: CAA62218.1.
X90647 Genomic DNA. Translation: CAA62219.1. Frameshift.
BC014753 mRNA. Translation: AAH14753.1.
CCDSiCCDS40460.1.
PIRiS60188.
RefSeqiNP_032315.2. NM_008289.2.
UniGeneiMm.5079.

Genome annotation databases

EnsembliENSMUST00000034363; ENSMUSP00000034363; ENSMUSG00000031891.
GeneIDi15484.
KEGGimmu:15484.
UCSCiuc009nde.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S79554
, S79550 , S79551 , S79552 , S79553 Genomic DNA. Translation: AAC60711.1 . Frameshift.
X90646 Genomic DNA. Translation: CAA62218.1 .
X90647 Genomic DNA. Translation: CAA62219.1 . Frameshift.
BC014753 mRNA. Translation: AAH14753.1 .
CCDSi CCDS40460.1.
PIRi S60188.
RefSeqi NP_032315.2. NM_008289.2.
UniGenei Mm.5079.

3D structure databases

ProteinModelPortali P51661.
SMRi P51661. Positions 81-339.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P51661. 3 interactions.
MINTi MINT-4093038.

Chemistry

BindingDBi P51661.
ChEMBLi CHEMBL3490.

PTM databases

PhosphoSitei P51661.

Proteomic databases

MaxQBi P51661.
PaxDbi P51661.
PRIDEi P51661.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000034363 ; ENSMUSP00000034363 ; ENSMUSG00000031891 .
GeneIDi 15484.
KEGGi mmu:15484.
UCSCi uc009nde.1. mouse.

Organism-specific databases

CTDi 3291.
MGIi MGI:104720. Hsd11b2.

Phylogenomic databases

eggNOGi COG1028.
GeneTreei ENSGT00770000120485.
HOVERGENi HBG005482.
InParanoidi P51661.
KOi K00071.
OMAi CFKTESV.
OrthoDBi EOG7FXZZX.
PhylomeDBi P51661.
TreeFami TF325617.

Enzyme and pathway databases

BRENDAi 1.1.1.146. 3474.

Miscellaneous databases

NextBioi 288342.
PROi P51661.
SOURCEi Search...

Gene expression databases

Bgeei P51661.
CleanExi MM_HSD11B2.
ExpressionAtlasi P51661. baseline and differential.
Genevestigatori P51661.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view ]
Pfami PF00106. adh_short. 1 hit.
[Graphical view ]
PRINTSi PR00081. GDHRDH.
PROSITEi PS00061. ADH_SHORT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of the mouse 11 beta-hydroxysteroid dehydrogenase type 2 gene: tissue specific expression and localization in distal convoluted tubules and collecting ducts of the kidney."
    Cole T.J.
    Endocrinology 136:4693-4696(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/Sv.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiDHI2_MOUSE
AccessioniPrimary (citable) accession number: P51661
Secondary accession number(s): Q91WK3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: April 30, 2003
Last modified: November 26, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3