Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P51660

- DHB4_MOUSE

UniProt

P51660 - DHB4_MOUSE

Protein

Peroxisomal multifunctional enzyme type 2

Gene

Hsd17b4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Bifunctional enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids. Catalyzes the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids By similarity.By similarity

    Catalytic activityi

    (R)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.
    (24R,25R)-3-alpha,7-alpha,12-alpha,24-tetrahydroxy-5-beta-cholestanoyl-CoA = (24E)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA + H2O.
    (3R)-3-hydroxyacyl-CoA = (2E)-2-enoyl-CoA + H2O.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei21 – 211NAD; via amide nitrogenBy similarity
    Binding sitei40 – 401NADBy similarity
    Binding sitei99 – 991NAD; via carbonyl oxygenBy similarity
    Binding sitei151 – 1511SubstrateBy similarity
    Active sitei164 – 1641Proton acceptorPROSITE-ProRule annotation
    Binding sitei434 – 4341(3R)-3-hydroxydecanoyl-CoABy similarity
    Binding sitei532 – 5321(3R)-3-hydroxydecanoyl-CoA; via amide nitrogenBy similarity
    Binding sitei562 – 5621(3R)-3-hydroxydecanoyl-CoA; via carbonyl oxygenBy similarity
    Binding sitei705 – 7051SubstrateBy similarity
    Binding sitei723 – 7231SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi13 – 3725NADBy similarityAdd
    BLAST
    Nucleotide bindingi75 – 762NADBy similarity
    Nucleotide bindingi164 – 1685NADBy similarity
    Nucleotide bindingi196 – 1994NADBy similarity

    GO - Molecular functioni

    1. 17-beta-hydroxysteroid dehydrogenase (NAD+) activity Source: Ensembl
    2. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA hydratase activity Source: UniProtKB-EC
    3. 3-hydroxyacyl-CoA dehydrogenase activity Source: Ensembl
    4. isomerase activity Source: UniProtKB-KW
    5. long-chain-enoyl-CoA hydratase activity Source: Ensembl

    GO - Biological processi

    1. androgen metabolic process Source: Ensembl
    2. estrogen metabolic process Source: Ensembl
    3. fatty acid beta-oxidation Source: MGI
    4. medium-chain fatty-acyl-CoA metabolic process Source: Ensembl
    5. Sertoli cell development Source: MGI
    6. very long-chain fatty acid metabolic process Source: MGI
    7. very long-chain fatty-acyl-CoA metabolic process Source: Ensembl

    Keywords - Molecular functioni

    Isomerase, Lyase, Oxidoreductase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    ReactomeiREACT_196541. alpha-linolenic acid (ALA) metabolism.
    REACT_203193. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
    UniPathwayiUPA00659.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Peroxisomal multifunctional enzyme type 2
    Short name:
    MFE-2
    Alternative name(s):
    17-beta-hydroxysteroid dehydrogenase 4
    Short name:
    17-beta-HSD 4
    D-bifunctional protein
    Short name:
    DBP
    Multifunctional protein 2
    Short name:
    MPF-2
    Cleaved into the following 2 chains:
    Alternative name(s):
    3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase
    Gene namesi
    Name:Hsd17b4
    Synonyms:Edh17b4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 18

    Organism-specific databases

    MGIiMGI:105089. Hsd17b4.

    Subcellular locationi

    Peroxisome By similarity

    GO - Cellular componenti

    1. mitochondrion Source: MGI
    2. peroxisomal membrane Source: Ensembl
    3. peroxisome Source: MGI

    Keywords - Cellular componenti

    Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 735735Peroxisomal multifunctional enzyme type 2PRO_0000054584Add
    BLAST
    Chaini1 – 311311(3R)-hydroxyacyl-CoA dehydrogenasePRO_0000400084Add
    BLAST
    Chaini312 – 735424Enoyl-CoA hydratase 2PRO_0000400085Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei46 – 461N6-acetyllysine; alternate1 Publication
    Modified residuei46 – 461N6-succinyllysine; alternate1 Publication
    Modified residuei57 – 571N6-succinyllysine1 Publication
    Modified residuei68 – 681N6-succinyllysine1 Publication
    Modified residuei84 – 841N6-succinyllysine1 Publication
    Modified residuei275 – 2751N6-succinyllysine1 Publication
    Modified residuei304 – 3041PhosphoserineBy similarity
    Modified residuei308 – 3081Phosphoserine1 Publication
    Modified residuei355 – 3551N6-succinyllysine1 Publication
    Modified residuei423 – 4231N6-succinyllysine1 Publication
    Modified residuei564 – 5641N6-acetyllysine1 Publication
    Modified residuei578 – 5781N6-succinyllysine1 Publication
    Modified residuei662 – 6621N6-succinyllysine1 Publication
    Modified residuei668 – 6681N6-acetyllysineBy similarity
    Modified residuei706 – 7061N6-acetyllysine1 Publication
    Modified residuei724 – 7241N6-succinyllysine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP51660.
    PaxDbiP51660.
    PRIDEiP51660.

    PTM databases

    PhosphoSiteiP51660.

    Expressioni

    Tissue specificityi

    Present in many tissues with highest concentrations in liver and kidney.

    Gene expression databases

    BgeeiP51660.
    CleanExiMM_HSD17B4.
    GenevestigatoriP51660.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    BioGridi200435. 1 interaction.
    IntActiP51660. 5 interactions.
    MINTiMINT-2512164.

    Structurei

    3D structure databases

    ProteinModelPortaliP51660.
    SMRiP51660. Positions 3-303, 322-604, 621-735.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini483 – 599117MaoC-likeAdd
    BLAST
    Domaini623 – 735113SCP2Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 305305(3R)-hydroxyacyl-CoA dehydrogenaseAdd
    BLAST
    Regioni321 – 621301Enoyl-CoA hydratase 2Add
    BLAST
    Regioni405 – 4062(3R)-3-hydroxydecanoyl-CoA bindingBy similarity
    Regioni509 – 5146(3R)-3-hydroxydecanoyl-CoA bindingBy similarity

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi733 – 7353Microbody targeting signalSequence Analysis

    Sequence similaritiesi

    Contains 1 MaoC-like domain.Curated
    Contains 1 SCP2 domain.Curated

    Phylogenomic databases

    eggNOGiCOG1028.
    GeneTreeiENSGT00530000062928.
    HOGENOMiHOG000170895.
    HOVERGENiHBG002174.
    InParanoidiP51660.
    KOiK12405.
    OMAiTETIMPP.
    OrthoDBiEOG7RBZ7V.
    PhylomeDBiP51660.
    TreeFamiTF105656.

    Family and domain databases

    Gene3Di3.10.129.10. 2 hits.
    3.30.1050.10. 1 hit.
    3.40.50.720. 1 hit.
    InterProiIPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR029069. HotDog_dom.
    IPR002539. MaoC_dom.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    IPR003033. SCP2_sterol-bd_dom.
    [Graphical view]
    PfamiPF00106. adh_short. 1 hit.
    PF01575. MaoC_dehydratas. 1 hit.
    PF02036. SCP2. 1 hit.
    [Graphical view]
    PRINTSiPR00081. GDHRDH.
    PR00080. SDRFAMILY.
    SUPFAMiSSF54637. SSF54637. 2 hits.
    SSF55718. SSF55718. 1 hit.
    PROSITEiPS00061. ADH_SHORT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P51660-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASPLRFDGR VVLVTGAGGG LGRAYALAFA ERGALVIVND LGGDFKGIGK    50
    GSSAADKVVA EIRRKGGKAV ANYDSVEAGE KLVKTALDTF GRIDVVVNNA 100
    GILRDRSFSR ISDEDWDIIH RVHLRGSFQV TRAAWDHMKK QNYGRILMTS 150
    SASGIYGNFG QANYSAAKLG ILGLCNTLAI EGRKNNIHCN TIAPNAGSRM 200
    TETVLPEDLV EALKPEYVAP LVLWLCHESC EENGGLFEVG AGWIGKLRWE 250
    RTLGAIVRKR NQPMTPEAVR DNWEKICDFS NASKPQTIQE STGGIVEVLH 300
    KVDSEGISPN RTSHAAPAAT SGFVGAVGHK LPSFSSSYTE LQSIMYALGV 350
    GASVKNPKDL KFVYEGSADF SCLPTFGVIV AQKSMMNGGL AEVPGLSFNF 400
    AKALHGEQYL ELYKPLPRSG ELKCEAVIAD ILDKGSGVVI VMDVYSYSGK 450
    ELICYNQFSV FVVGSGGFGG KRTSEKLKAA VAVPNRPPDA VLRDATSLNQ 500
    AALYRLSGDW NPLHIDPDFA SVAGFEKPIL HGLCTFGFSA RHVLQQFADN 550
    DVSRFKAIKV RFAKPVYPGQ TLQTEMWKEG NRIHFQTKVH ETGDVVISNA 600
    YVDLVPASGV STQTPSEGGE LQSALVFGEI GRRLKSVGRE VVKKANAVFE 650
    WHITKGGTVA AKWTIDLKSG SGEVYQGPAK GSADVTIIIS DEDFMEVVFG 700
    KLDPQKAFFS GRLKARGNIM LSQKLQMILK DYAKL 735
    Length:735
    Mass (Da):79,482
    Last modified:January 23, 2007 - v3
    Checksum:iAD7804FE93EB9BA8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti17 – 171A → P in CAA62015. (PubMed:8547180)Curated
    Sequence conflicti417 – 4171P → L in CAA62015. (PubMed:8547180)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X89998 mRNA. Translation: CAA62015.1.
    AK004866 mRNA. Translation: BAB23627.1.
    AK088381 mRNA. Translation: BAC40317.1.
    AK166801 mRNA. Translation: BAE39029.1.
    AK167060 mRNA. Translation: BAE39222.1.
    AK169077 mRNA. Translation: BAE40862.1.
    BC022175 mRNA. Translation: AAH22175.1.
    CCDSiCCDS29242.1.
    RefSeqiNP_032318.2. NM_008292.4.
    UniGeneiMm.277857.

    Genome annotation databases

    EnsembliENSMUST00000025385; ENSMUSP00000025385; ENSMUSG00000024507.
    GeneIDi15488.
    KEGGimmu:15488.
    UCSCiuc008eww.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X89998 mRNA. Translation: CAA62015.1 .
    AK004866 mRNA. Translation: BAB23627.1 .
    AK088381 mRNA. Translation: BAC40317.1 .
    AK166801 mRNA. Translation: BAE39029.1 .
    AK167060 mRNA. Translation: BAE39222.1 .
    AK169077 mRNA. Translation: BAE40862.1 .
    BC022175 mRNA. Translation: AAH22175.1 .
    CCDSi CCDS29242.1.
    RefSeqi NP_032318.2. NM_008292.4.
    UniGenei Mm.277857.

    3D structure databases

    ProteinModelPortali P51660.
    SMRi P51660. Positions 3-303, 322-604, 621-735.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200435. 1 interaction.
    IntActi P51660. 5 interactions.
    MINTi MINT-2512164.

    PTM databases

    PhosphoSitei P51660.

    Proteomic databases

    MaxQBi P51660.
    PaxDbi P51660.
    PRIDEi P51660.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000025385 ; ENSMUSP00000025385 ; ENSMUSG00000024507 .
    GeneIDi 15488.
    KEGGi mmu:15488.
    UCSCi uc008eww.2. mouse.

    Organism-specific databases

    CTDi 3295.
    MGIi MGI:105089. Hsd17b4.

    Phylogenomic databases

    eggNOGi COG1028.
    GeneTreei ENSGT00530000062928.
    HOGENOMi HOG000170895.
    HOVERGENi HBG002174.
    InParanoidi P51660.
    KOi K12405.
    OMAi TETIMPP.
    OrthoDBi EOG7RBZ7V.
    PhylomeDBi P51660.
    TreeFami TF105656.

    Enzyme and pathway databases

    UniPathwayi UPA00659 .
    Reactomei REACT_196541. alpha-linolenic acid (ALA) metabolism.
    REACT_203193. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.

    Miscellaneous databases

    ChiTaRSi HSD17B4. mouse.
    NextBioi 288358.
    PROi P51660.
    SOURCEi Search...

    Gene expression databases

    Bgeei P51660.
    CleanExi MM_HSD17B4.
    Genevestigatori P51660.

    Family and domain databases

    Gene3Di 3.10.129.10. 2 hits.
    3.30.1050.10. 1 hit.
    3.40.50.720. 1 hit.
    InterProi IPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR029069. HotDog_dom.
    IPR002539. MaoC_dom.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    IPR003033. SCP2_sterol-bd_dom.
    [Graphical view ]
    Pfami PF00106. adh_short. 1 hit.
    PF01575. MaoC_dehydratas. 1 hit.
    PF02036. SCP2. 1 hit.
    [Graphical view ]
    PRINTSi PR00081. GDHRDH.
    PR00080. SDRFAMILY.
    SUPFAMi SSF54637. SSF54637. 2 hits.
    SSF55718. SSF55718. 1 hit.
    PROSITEi PS00061. ADH_SHORT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
      Tissue: Amnion, Liver and Thymus.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Colon.
    4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-308, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    5. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-46; LYS-57; LYS-68; LYS-84; LYS-275; LYS-355; LYS-423; LYS-578; LYS-662 AND LYS-724, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    6. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
      Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
      Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-46; LYS-564 AND LYS-706, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiDHB4_MOUSE
    AccessioniPrimary (citable) accession number: P51660
    Secondary accession number(s): Q9DBM3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 128 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The protein is found both as a full length peptide and in a cleaved version.By similarity

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3