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Protein

Peroxisomal multifunctional enzyme type 2

Gene

Hsd17b4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Bifunctional enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids. Catalyzes the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids (By similarity).By similarity

Catalytic activityi

(R)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.
(24R,25R)-3-alpha,7-alpha,12-alpha,24-tetrahydroxy-5-beta-cholestanoyl-CoA = (24E)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA + H2O.
(3R)-3-hydroxyacyl-CoA = (2E)-2-enoyl-CoA + H2O.

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei21NAD; via amide nitrogenBy similarity1
Binding sitei40NADBy similarity1
Binding sitei99NAD; via carbonyl oxygenBy similarity1
Binding sitei151SubstrateBy similarity1
Active sitei164Proton acceptorPROSITE-ProRule annotation1
Binding sitei434(3R)-3-hydroxydecanoyl-CoABy similarity1
Binding sitei532(3R)-3-hydroxydecanoyl-CoA; via amide nitrogenBy similarity1
Binding sitei562(3R)-3-hydroxydecanoyl-CoA; via carbonyl oxygenBy similarity1
Binding sitei705SubstrateBy similarity1
Binding sitei723SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 37NADBy similarityAdd BLAST25
Nucleotide bindingi75 – 76NADBy similarity2
Nucleotide bindingi164 – 168NADBy similarity5
Nucleotide bindingi196 – 199NADBy similarity4

GO - Molecular functioni

GO - Biological processi

  • androgen metabolic process Source: MGI
  • estrogen metabolic process Source: MGI
  • fatty acid beta-oxidation Source: MGI
  • medium-chain fatty-acyl-CoA metabolic process Source: MGI
  • osteoblast differentiation Source: MGI
  • Sertoli cell development Source: MGI
  • very long-chain fatty acid metabolic process Source: MGI
  • very long-chain fatty-acyl-CoA metabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase, Oxidoreductase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiR-MMU-193368. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
R-MMU-2046106. alpha-linolenic acid (ALA) metabolism.
R-MMU-389887. Beta-oxidation of pristanoyl-CoA.
R-MMU-390247. Beta-oxidation of very long chain fatty acids.
UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal multifunctional enzyme type 2
Short name:
MFE-2
Alternative name(s):
17-beta-hydroxysteroid dehydrogenase 4
Short name:
17-beta-HSD 4
D-bifunctional protein
Short name:
DBP
Multifunctional protein 2
Short name:
MPF-2
Cleaved into the following 2 chains:
Alternative name(s):
3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase
Gene namesi
Name:Hsd17b4
Synonyms:Edh17b4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:105089. Hsd17b4.

Subcellular locationi

GO - Cellular componenti

  • intracellular membrane-bounded organelle Source: MGI
  • membrane Source: MGI
  • mitochondrion Source: MGI
  • peroxisomal membrane Source: MGI
  • peroxisome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3621031.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000545841 – 735Peroxisomal multifunctional enzyme type 2Add BLAST735
ChainiPRO_00004000841 – 311(3R)-hydroxyacyl-CoA dehydrogenaseAdd BLAST311
ChainiPRO_0000400085312 – 735Enoyl-CoA hydratase 2Add BLAST424

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei46N6-acetyllysine; alternateCombined sources1
Modified residuei46N6-succinyllysine; alternateCombined sources1
Modified residuei52PhosphoserineBy similarity1
Modified residuei57N6-succinyllysineCombined sources1
Modified residuei68N6-succinyllysineCombined sources1
Modified residuei84N6-succinyllysineCombined sources1
Modified residuei265PhosphothreonineBy similarity1
Modified residuei275N6-succinyllysineCombined sources1
Modified residuei304PhosphoserineBy similarity1
Modified residuei308PhosphoserineCombined sources1
Modified residuei355N6-succinyllysineCombined sources1
Modified residuei423N6-succinyllysineCombined sources1
Modified residuei564N6-acetyllysineCombined sources1
Modified residuei578N6-succinyllysineCombined sources1
Modified residuei662N6-succinyllysineCombined sources1
Modified residuei668N6-acetyllysineBy similarity1
Modified residuei706N6-acetyllysineCombined sources1
Modified residuei724N6-succinyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP51660.
MaxQBiP51660.
PaxDbiP51660.
PeptideAtlasiP51660.
PRIDEiP51660.

PTM databases

iPTMnetiP51660.
PhosphoSitePlusiP51660.
SwissPalmiP51660.

Expressioni

Tissue specificityi

Present in many tissues with highest concentrations in liver and kidney.

Gene expression databases

BgeeiENSMUSG00000024507.
CleanExiMM_HSD17B4.
GenevisibleiP51660. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200435. 1 interactor.
IntActiP51660. 5 interactors.
MINTiMINT-2512164.
STRINGi10090.ENSMUSP00000025385.

Structurei

3D structure databases

ProteinModelPortaliP51660.
SMRiP51660.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini483 – 599MaoC-likeAdd BLAST117
Domaini623 – 735SCP2Add BLAST113

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 305(3R)-hydroxyacyl-CoA dehydrogenaseAdd BLAST305
Regioni321 – 621Enoyl-CoA hydratase 2Add BLAST301
Regioni405 – 406(3R)-3-hydroxydecanoyl-CoA bindingBy similarity2
Regioni509 – 514(3R)-3-hydroxydecanoyl-CoA bindingBy similarity6

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi733 – 735Microbody targeting signalSequence analysis3

Sequence similaritiesi

Contains 1 MaoC-like domain.Curated
Contains 1 SCP2 domain.Curated

Phylogenomic databases

eggNOGiKOG1206. Eukaryota.
COG2030. LUCA.
GeneTreeiENSGT00530000062928.
HOGENOMiHOG000170895.
HOVERGENiHBG002174.
InParanoidiP51660.
KOiK12405.
OMAiTVMPEDL.
OrthoDBiEOG091G02S9.
PhylomeDBiP51660.
TreeFamiTF105656.

Family and domain databases

Gene3Di3.10.129.10. 2 hits.
3.30.1050.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR029069. HotDog_dom.
IPR002539. MaoC_dom.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR003033. SCP2_sterol-bd_dom.
IPR002347. SDR_fam.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
PF01575. MaoC_dehydratas. 1 hit.
PF02036. SCP2. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF54637. SSF54637. 2 hits.
SSF55718. SSF55718. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P51660-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASPLRFDGR VVLVTGAGGG LGRAYALAFA ERGALVIVND LGGDFKGIGK
60 70 80 90 100
GSSAADKVVA EIRRKGGKAV ANYDSVEAGE KLVKTALDTF GRIDVVVNNA
110 120 130 140 150
GILRDRSFSR ISDEDWDIIH RVHLRGSFQV TRAAWDHMKK QNYGRILMTS
160 170 180 190 200
SASGIYGNFG QANYSAAKLG ILGLCNTLAI EGRKNNIHCN TIAPNAGSRM
210 220 230 240 250
TETVLPEDLV EALKPEYVAP LVLWLCHESC EENGGLFEVG AGWIGKLRWE
260 270 280 290 300
RTLGAIVRKR NQPMTPEAVR DNWEKICDFS NASKPQTIQE STGGIVEVLH
310 320 330 340 350
KVDSEGISPN RTSHAAPAAT SGFVGAVGHK LPSFSSSYTE LQSIMYALGV
360 370 380 390 400
GASVKNPKDL KFVYEGSADF SCLPTFGVIV AQKSMMNGGL AEVPGLSFNF
410 420 430 440 450
AKALHGEQYL ELYKPLPRSG ELKCEAVIAD ILDKGSGVVI VMDVYSYSGK
460 470 480 490 500
ELICYNQFSV FVVGSGGFGG KRTSEKLKAA VAVPNRPPDA VLRDATSLNQ
510 520 530 540 550
AALYRLSGDW NPLHIDPDFA SVAGFEKPIL HGLCTFGFSA RHVLQQFADN
560 570 580 590 600
DVSRFKAIKV RFAKPVYPGQ TLQTEMWKEG NRIHFQTKVH ETGDVVISNA
610 620 630 640 650
YVDLVPASGV STQTPSEGGE LQSALVFGEI GRRLKSVGRE VVKKANAVFE
660 670 680 690 700
WHITKGGTVA AKWTIDLKSG SGEVYQGPAK GSADVTIIIS DEDFMEVVFG
710 720 730
KLDPQKAFFS GRLKARGNIM LSQKLQMILK DYAKL
Length:735
Mass (Da):79,482
Last modified:January 23, 2007 - v3
Checksum:iAD7804FE93EB9BA8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17A → P in CAA62015 (PubMed:8547180).Curated1
Sequence conflicti417P → L in CAA62015 (PubMed:8547180).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89998 mRNA. Translation: CAA62015.1.
AK004866 mRNA. Translation: BAB23627.1.
AK088381 mRNA. Translation: BAC40317.1.
AK166801 mRNA. Translation: BAE39029.1.
AK167060 mRNA. Translation: BAE39222.1.
AK169077 mRNA. Translation: BAE40862.1.
BC022175 mRNA. Translation: AAH22175.1.
CCDSiCCDS29242.1.
RefSeqiNP_032318.2. NM_008292.4.
UniGeneiMm.277857.

Genome annotation databases

EnsembliENSMUST00000025385; ENSMUSP00000025385; ENSMUSG00000024507.
GeneIDi15488.
KEGGimmu:15488.
UCSCiuc008eww.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89998 mRNA. Translation: CAA62015.1.
AK004866 mRNA. Translation: BAB23627.1.
AK088381 mRNA. Translation: BAC40317.1.
AK166801 mRNA. Translation: BAE39029.1.
AK167060 mRNA. Translation: BAE39222.1.
AK169077 mRNA. Translation: BAE40862.1.
BC022175 mRNA. Translation: AAH22175.1.
CCDSiCCDS29242.1.
RefSeqiNP_032318.2. NM_008292.4.
UniGeneiMm.277857.

3D structure databases

ProteinModelPortaliP51660.
SMRiP51660.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200435. 1 interactor.
IntActiP51660. 5 interactors.
MINTiMINT-2512164.
STRINGi10090.ENSMUSP00000025385.

Chemistry databases

ChEMBLiCHEMBL3621031.

PTM databases

iPTMnetiP51660.
PhosphoSitePlusiP51660.
SwissPalmiP51660.

Proteomic databases

EPDiP51660.
MaxQBiP51660.
PaxDbiP51660.
PeptideAtlasiP51660.
PRIDEiP51660.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025385; ENSMUSP00000025385; ENSMUSG00000024507.
GeneIDi15488.
KEGGimmu:15488.
UCSCiuc008eww.2. mouse.

Organism-specific databases

CTDi3295.
MGIiMGI:105089. Hsd17b4.

Phylogenomic databases

eggNOGiKOG1206. Eukaryota.
COG2030. LUCA.
GeneTreeiENSGT00530000062928.
HOGENOMiHOG000170895.
HOVERGENiHBG002174.
InParanoidiP51660.
KOiK12405.
OMAiTVMPEDL.
OrthoDBiEOG091G02S9.
PhylomeDBiP51660.
TreeFamiTF105656.

Enzyme and pathway databases

UniPathwayiUPA00659.
ReactomeiR-MMU-193368. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
R-MMU-2046106. alpha-linolenic acid (ALA) metabolism.
R-MMU-389887. Beta-oxidation of pristanoyl-CoA.
R-MMU-390247. Beta-oxidation of very long chain fatty acids.

Miscellaneous databases

ChiTaRSiHsd17b4. mouse.
PROiP51660.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024507.
CleanExiMM_HSD17B4.
GenevisibleiP51660. MM.

Family and domain databases

Gene3Di3.10.129.10. 2 hits.
3.30.1050.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR029069. HotDog_dom.
IPR002539. MaoC_dom.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR003033. SCP2_sterol-bd_dom.
IPR002347. SDR_fam.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
PF01575. MaoC_dehydratas. 1 hit.
PF02036. SCP2. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF54637. SSF54637. 2 hits.
SSF55718. SSF55718. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDHB4_MOUSE
AccessioniPrimary (citable) accession number: P51660
Secondary accession number(s): Q9DBM3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 149 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The protein is found both as a full length peptide and in a cleaved version.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.