Reviewed, UniProtKB/Swiss-Prot P51658 (DHB2_MOUSE)

Names and origin

Protein names

Recommended name:
    Estradiol 17-beta-dehydrogenase 2
    EC=1.1.1.62
Alternative name(s):
    Testosterone 17-beta-dehydrogenase
    EC=1.1.1.63
    17-beta-hydroxysteroid dehydrogenase 2
      Short name=17-beta-HSD 2

Gene names

Name:	Hsd17b2
Synonyms:	Edh17b2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH By similarity.
Catalytic activity	Estradiol-17-beta + NAD(P)+ = estrone + NAD(P)H. Testosterone + NAD+ = androst-4-ene-3,17-dione + NADH.
Subcellular location	Membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis
Cellular component	Membrane
Domain	Signal-anchor Transmembrane
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro estradiol 17-beta-dehydrogenase activity Inferred from electronic annotation. Source: EC testosterone 17-beta-dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 381	381	Estradiol 17-beta-dehydrogenase 2		PRO_0000054571
Regions
	Transmembrane	4 – 24	21	Signal-anchor for type II membrane protein Potential
	Nucleotide binding	83 – 112	30	NAD By similarity
Sites
	Active site	233	1	Proton acceptor By similarity
	Binding site	220	1	Substrate By similarity
Experimental info
	Sequence conflict	36 – 37	2	QA → RP in CAA64982. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P51658-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 74A62797947E6086	FASTA	381	41,836
	10 20 30 40 50 60 MSPFASESAW LCLAAAAVLG GTLLCGCRSG RQLRSQAVCL AGLWGGACLL SLSLLCTLFL 70 80 90 100 110 120 LSVACFLLLY MSSSDQDLLP VDQKAVLVTG ADSGFGHGLA KHLDKLGFTV FAGVLDKEGP 130 140 150 160 170 180 GAEELRKHCS ERLSVLQMDV TKPEQIKDAH SKVTEKIQDK GLWAVVNNAG VFHLPIDGEL 190 200 210 220 230 240 IPMSIYRKCM AVNFFGTVEV TKAFLPLLRK SKGRLVNVSS MGGTVPLQMT SAYAATKAAL 250 260 270 280 290 300 TMFSTIIRQE LDKWGVKVVT IKPGGFKTNI TGSQDIWDKM EKEILDHFSK DIQENYGQDY 310 320 330 340 350 360 VHTQKLIIPT LKERSNPDIT PVLRDIQHAI SARNPSSFYY PGRMAYLWVC LAAYCPTSLL 370 380 DYVIKKGFYP QPTPRALRTV H

References

[1]	"Cloning of mouse 17beta-hydroxysteroid dehydrogenase type 2, and analysing expression of the mRNAs for types 1, 2, 3, 4 and 5 in mouse embryos and adult tissues." Mustonen M. Biochem. J. 325:199-205(1997) [PubMed: 9224647] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Liver.
[2]	Stoffel W., Weiss B. Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-358. Strain: BALB/c.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y09517 mRNA. Translation: CAA70706.1. X95685 mRNA. Translation: CAA64982.1.
IPI	IPI00125076.
RefSeq	NP_032316.2.
UniGene	Mm.276466
3D structure databases
HSSP	HSSP built from PDB template 1FDS based on UniProtKB P14061.
ModBase	Search...
Proteomic databases
PRIDE	P51658.
Genome annotation databases
Ensembl	ENSMUSG00000031844. Mus musculus. [Contig view]
GeneID	15486.
KEGG	mmu:15486.
Organism-specific databases
MGI	MGI:1096386. Hsd17b2.
Phylogenomic databases
HOGENOM	P51658.
HOVERGEN	P51658.
OMA	P51658. QEDYGQD.
Enzyme and pathway databases
BRENDA	1.1.1.62. 244. 1.1.1.63. 244.
Gene expression databases
ArrayExpress	P51658.
Bgee	P51658.
CleanEx	MM_HSD17B2.
GermOnline	ENSMUSG00000031844. Mus musculus.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	288350.
SOURCE	Search...

Entry information

Entry name

DHB2_MOUSE

Accession

Primary (citable) accession number: P51658
Secondary accession number(s): O08898

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)