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Protein

Estradiol 17-beta-dehydrogenase 2

Gene

Hsd17b2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH (By similarity).By similarity

Catalytic activityi

17-beta-estradiol + NAD(P)+ = estrone + NAD(P)H.
Testosterone + NAD+ = androstenedione + NADH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei220 – 2201SubstrateBy similarity
Active sitei233 – 2331Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi83 – 11230NADBy similarityAdd
BLAST

GO - Molecular functioni

  1. estradiol 17-beta-dehydrogenase activity Source: MGI
  2. testosterone dehydrogenase (NAD+) activity Source: MGI

GO - Biological processi

  1. in utero embryonic development Source: MGI
  2. placenta development Source: MGI
  3. response to retinoic acid Source: MGI
  4. steroid biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Estradiol 17-beta-dehydrogenase 2 (EC:1.1.1.62)
Alternative name(s):
17-beta-hydroxysteroid dehydrogenase type 2
Short name:
17-beta-HSD 2
Testosterone 17-beta-dehydrogenase (EC:1.1.1.239)
Gene namesi
Name:Hsd17b2
Synonyms:Edh17b2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:1096386. Hsd17b2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei4 – 2421Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 381381Estradiol 17-beta-dehydrogenase 2PRO_0000054571Add
BLAST

Proteomic databases

MaxQBiP51658.
PaxDbiP51658.
PRIDEiP51658.

PTM databases

PhosphoSiteiP51658.

Expressioni

Gene expression databases

BgeeiP51658.
CleanExiMM_HSD17B2.
ExpressionAtlasiP51658. baseline and differential.
GenevestigatoriP51658.

Interactioni

Protein-protein interaction databases

MINTiMINT-1864098.
STRINGi10090.ENSMUSP00000034304.

Structurei

3D structure databases

ProteinModelPortaliP51658.
SMRiP51658. Positions 83-324.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1028.
GeneTreeiENSGT00770000120485.
HOVERGENiHBG005482.
InParanoidiP51658.
KOiK13368.
OMAiQEDYGQD.
OrthoDBiEOG7FXZZX.
PhylomeDBiP51658.
TreeFamiTF325617.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P51658-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPFASESAW LCLAAAAVLG GTLLCGCRSG RQLRSQAVCL AGLWGGACLL
60 70 80 90 100
SLSLLCTLFL LSVACFLLLY MSSSDQDLLP VDQKAVLVTG ADSGFGHGLA
110 120 130 140 150
KHLDKLGFTV FAGVLDKEGP GAEELRKHCS ERLSVLQMDV TKPEQIKDAH
160 170 180 190 200
SKVTEKIQDK GLWAVVNNAG VFHLPIDGEL IPMSIYRKCM AVNFFGTVEV
210 220 230 240 250
TKAFLPLLRK SKGRLVNVSS MGGTVPLQMT SAYAATKAAL TMFSTIIRQE
260 270 280 290 300
LDKWGVKVVT IKPGGFKTNI TGSQDIWDKM EKEILDHFSK DIQENYGQDY
310 320 330 340 350
VHTQKLIIPT LKERSNPDIT PVLRDIQHAI SARNPSSFYY PGRMAYLWVC
360 370 380
LAAYCPTSLL DYVIKKGFYP QPTPRALRTV H
Length:381
Mass (Da):41,836
Last modified:November 1, 1997 - v2
Checksum:i74A62797947E6086
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti36 – 372QA → RP in CAA64982 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09517 mRNA. Translation: CAA70706.1.
X95685 mRNA. Translation: CAA64982.1.
CCDSiCCDS40491.1.
RefSeqiNP_032316.2. NM_008290.2.
UniGeneiMm.276466.

Genome annotation databases

EnsembliENSMUST00000034304; ENSMUSP00000034304; ENSMUSG00000031844.
GeneIDi15486.
KEGGimmu:15486.
UCSCiuc009npf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09517 mRNA. Translation: CAA70706.1.
X95685 mRNA. Translation: CAA64982.1.
CCDSiCCDS40491.1.
RefSeqiNP_032316.2. NM_008290.2.
UniGeneiMm.276466.

3D structure databases

ProteinModelPortaliP51658.
SMRiP51658. Positions 83-324.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1864098.
STRINGi10090.ENSMUSP00000034304.

Chemistry

ChEMBLiCHEMBL1914270.

PTM databases

PhosphoSiteiP51658.

Proteomic databases

MaxQBiP51658.
PaxDbiP51658.
PRIDEiP51658.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034304; ENSMUSP00000034304; ENSMUSG00000031844.
GeneIDi15486.
KEGGimmu:15486.
UCSCiuc009npf.1. mouse.

Organism-specific databases

CTDi3294.
MGIiMGI:1096386. Hsd17b2.

Phylogenomic databases

eggNOGiCOG1028.
GeneTreeiENSGT00770000120485.
HOVERGENiHBG005482.
InParanoidiP51658.
KOiK13368.
OMAiQEDYGQD.
OrthoDBiEOG7FXZZX.
PhylomeDBiP51658.
TreeFamiTF325617.

Miscellaneous databases

NextBioi288350.
PROiP51658.
SOURCEiSearch...

Gene expression databases

BgeeiP51658.
CleanExiMM_HSD17B2.
ExpressionAtlasiP51658. baseline and differential.
GenevestigatoriP51658.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning of mouse 17beta-hydroxysteroid dehydrogenase type 2, and analysing expression of the mRNAs for types 1, 2, 3, 4 and 5 in mouse embryos and adult tissues."
    Mustonen M.
    Biochem. J. 325:199-205(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Liver.
  2. Stoffel W., Weiss B.
    Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-358.
    Strain: BALB/c.

Entry informationi

Entry nameiDHB2_MOUSE
AccessioniPrimary (citable) accession number: P51658
Secondary accession number(s): O08898
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 1, 1997
Last modified: February 4, 2015
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.