ID   DHB1_RAT                Reviewed;         344 AA.
AC   P51657;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   16-JUN-2009, entry version 68.
DE   RecName: Full=Estradiol 17-beta-dehydrogenase 1;
DE            EC=1.1.1.62;
DE   AltName: Full=17-beta-hydroxysteroid dehydrogenase type 1;
DE            Short=17-beta-HSD 1;
GN   Name=Hsd17b1; Synonyms=Edh17b1;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND CATALYTIC ACTIVITY.
RC   STRAIN=Sprague-Dawley; TISSUE=Ovary;
RX   MEDLINE=95009707; PubMed=7925110; DOI=10.1210/en.135.4.1477;
RA   Ghersevich S., Nokelainen P., Poutanen M., Orava M.,
RA   Autio-Harmainen H., Rajaniemi H., Vihko R.;
RT   "Rat 17 beta-hydroxysteroid dehydrogenase type 1: primary structure
RT   and regulation of enzyme expression in rat ovary by diethylstilbestrol
RT   and gonadotropins in vivo.";
RL   Endocrinology 135:1477-1487(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Sprague-Dawley; TISSUE=Kidney;
RX   MEDLINE=96198748; PubMed=8612487; DOI=10.1210/en.137.5.1572;
RA   Akinola L.A., Poutanen M., Vihko R.;
RT   "Cloning of rat 17 beta-hydroxysteroid dehydrogenase type 2 and
RT   characterization of tissue distribution and catalytic activity of rat
RT   type 1 and type 2 enzymes.";
RL   Endocrinology 137:1572-1579(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Sprague-Dawley; TISSUE=Testis;
RX   MEDLINE=98201618; PubMed=9524272; DOI=10.1016/S0378-1119(97)00669-0;
RA   Akinola L.A., Poutanen M., Peltoketo H., Vihko R., Vihko P.;
RT   "Characterization of rat 17 beta-hydroxysteroid dehydrogenase type 1
RT   gene and mRNA transcripts.";
RL   Gene 208:229-238(1998).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Ovary;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Favors the reduction of estrogens and androgens. Uses
CC       preferentially NADH.
CC   -!- CATALYTIC ACTIVITY: Estradiol-17-beta + NAD(P)(+) = estrone +
CC       NAD(P)H.
CC   -!- PATHWAY: Steroid biosynthesis; estrogen biosynthesis.
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases
CC       (SDR) family.
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DR   EMBL; X78811; CAA55389.1; -; mRNA.
DR   EMBL; X97754; CAA66349.1; -; mRNA.
DR   EMBL; X98038; CAA66657.1; -; Genomic_DNA.
DR   EMBL; BC086365; AAH86365.1; -; mRNA.
DR   IPI; IPI00204305; -.
DR   PIR; S57901; S57901.
DR   RefSeq; NP_036983.1; -.
DR   UniGene; Rn.10594; -.
DR   HSSP; P14061; 1FDW.
DR   SMR; P51657; 4-284.
DR   PRIDE; P51657; -.
DR   Ensembl; ENSRNOG00000019830; Rattus norvegicus.
DR   GeneID; 25322; -.
DR   KEGG; rno:25322; -.
DR   RGD; 2836; Hsd17b1.
DR   HOVERGEN; P51657; -.
DR   BRENDA; 1.1.1.62; 248.
DR   NextBio; 606169; -.
DR   ArrayExpress; P51657; -.
DR   GermOnline; ENSRNOG00000019830; Rattus norvegicus.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0004303; F:estradiol 17-beta-dehydrogenase activity; TAS:RGD.
DR   GO; GO:0006703; P:estrogen biosynthetic process; NAS:RGD.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR011348; 17beta_DH.
DR   InterPro; IPR002198; DH_sc/Rdtase_SDR.
DR   InterPro; IPR002347; Glc/ribitol_DH.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR19410:SF47; 17beta_DH; 1.
DR   PANTHER; PTHR19410; ADH_short_C2; 1.
DR   Pfam; PF00106; adh_short; 1.
DR   PIRSF; PIRSF000095; 17beta-HSD; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Lipid synthesis; NAD; Oxidoreductase; Steroid biosynthesis.
FT   CHAIN         1    344       Estradiol 17-beta-dehydrogenase 1.
FT                                /FTId=PRO_0000054569.
FT   NP_BIND       3     32       NAD (By similarity).
FT   ACT_SITE    156    156       Proton acceptor (By similarity).
FT   BINDING     143    143       Substrate (By similarity).
SQ   SEQUENCE   344 AA;  36967 MW;  CB5FC139FBB3995E CRC64;
     MDSTVVLITG CSSGIGLHLA VRLASDRSQS FKVYATLRDL KSQGPLLEAA RAQGCPPGSL
     EILELDVRDS ESVAAARACV TEGRVDVLVC NAGRGLFGPL EAHELNAVGA VLDVNVLGTI
     RMLQAFLPDM KRRHSGRVLV TASVGGLMGL PFHEVYCASK FALEGLCESL AILLPLFGVH
     VSLIECGAVH TAFHEKLEGG PGGALERADA QTRHLFAHYQ RGYEQALSEA QDPEEVTELF
     LTAMRAPQPA LRYFSTNRFL PLARMRTEDP SGSSYVEAMH REAFSDLQVQ EGAKAGAQVS
     GDPDTPPRAL ICLPECAIPR VTAELGWSAS DKPGQNKSCY QQKI
//