Reviewed, UniProtKB/Swiss-Prot P51657 (DHB1_RAT)

Names and origin

Protein names

Recommended name:
    Estradiol 17-beta-dehydrogenase 1
    EC=1.1.1.62
Alternative name(s):
    17-beta-hydroxysteroid dehydrogenase type 1
      Short name=17-beta-HSD 1

Gene names

Name:	Hsd17b1
Synonyms:	Edh17b1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Favors the reduction of estrogens and androgens. Uses preferentially NADH.
Catalytic activity	Estradiol-17-beta + NAD(P)+ = estrone + NAD(P)H. Ref.1
Pathway	Steroid biosynthesis; estrogen biosynthesis.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	estrogen biosynthetic process Ref.2 Non-traceable author statement. Source: RGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	binding Inferred from electronic annotation. Source: InterPro estradiol 17-beta-dehydrogenase activity Ref.2 Traceable author statement. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Estradiol 17-beta-dehydrogenase 1		PRO_0000054569
Regions
	Nucleotide binding	3 – 32	30	NAD By similarity
Sites
	Active site	156	1	Proton acceptor By similarity
	Binding site	143	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P51657-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: CB5FC139FBB3995E	FASTA	344	36,967
	10 20 30 40 50 60 MDSTVVLITG CSSGIGLHLA VRLASDRSQS FKVYATLRDL KSQGPLLEAA RAQGCPPGSL 70 80 90 100 110 120 EILELDVRDS ESVAAARACV TEGRVDVLVC NAGRGLFGPL EAHELNAVGA VLDVNVLGTI 130 140 150 160 170 180 RMLQAFLPDM KRRHSGRVLV TASVGGLMGL PFHEVYCASK FALEGLCESL AILLPLFGVH 190 200 210 220 230 240 VSLIECGAVH TAFHEKLEGG PGGALERADA QTRHLFAHYQ RGYEQALSEA QDPEEVTELF 250 260 270 280 290 300 LTAMRAPQPA LRYFSTNRFL PLARMRTEDP SGSSYVEAMH REAFSDLQVQ EGAKAGAQVS 310 320 330 340 GDPDTPPRAL ICLPECAIPR VTAELGWSAS DKPGQNKSCY QQKI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rat 17 beta-hydroxysteroid dehydrogenase type 1: primary structure and regulation of enzyme expression in rat ovary by diethylstilbestrol and gonadotropins in vivo." Ghersevich S., Nokelainen P., Poutanen M., Orava M., Autio-Harmainen H., Rajaniemi H., Vihko R. Endocrinology 135:1477-1487(1994) [PubMed: 7925110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY. Strain: Sprague-Dawley. Tissue: Ovary.
[2]	"Cloning of rat 17 beta-hydroxysteroid dehydrogenase type 2 and characterization of tissue distribution and catalytic activity of rat type 1 and type 2 enzymes." Akinola L.A., Poutanen M., Vihko R. Endocrinology 137:1572-1579(1996) [PubMed: 8612487] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Kidney.
[3]	"Characterization of rat 17 beta-hydroxysteroid dehydrogenase type 1 gene and mRNA transcripts." Akinola L.A., Poutanen M., Peltoketo H., Vihko R., Vihko P. Gene 208:229-238(1998) [PubMed: 9524272] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sprague-Dawley. Tissue: Testis.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X78811 mRNA. Translation: CAA55389.1. X97754 mRNA. Translation: CAA66349.1. X98038 Genomic DNA. Translation: CAA66657.1. BC086365 mRNA. Translation: AAH86365.1.
IPI	IPI00204305.
PIR	S57901.
RefSeq	NP_036983.1.
UniGene	Rn.10594
3D structure databases
HSSP	HSSP built from PDB template 1FDW based on UniProtKB P14061.
SMR	P51657. Positions 4-284.
ModBase	Search...
Proteomic databases
PRIDE	P51657.
Genome annotation databases
Ensembl	ENSRNOG00000019830. Rattus norvegicus. [Contig view]
GeneID	25322.
KEGG	rno:25322.
Organism-specific databases
RGD	2836. Hsd17b1.
Phylogenomic databases
HOVERGEN	P51657.
Enzyme and pathway databases
BRENDA	1.1.1.62. 248.
Gene expression databases
ArrayExpress	P51657.
GermOnline	ENSRNOG00000019830. Rattus norvegicus.
Family and domain databases
InterPro	IPR011348. 17beta_DH. IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410:SF47. 17beta_DH. 1 hit. PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PIRSF	PIRSF000095. 17beta-HSD. 1 hit.
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	606169.

Entry information

Entry name

DHB1_RAT

Accession

Primary (citable) accession number: P51657

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)